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1 Chapter 8
2 TEL GHSGlobal Harmonized System WEBwww.lifetechnologies.com/cartagena/ TEL MSDSMaterial Safety Data Sheet MSDSwww.lifetechnologies.com TEL DNAcDNA Life Technologies, Carlsbad, CA USA TEL i
3 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
4 Cell Biology Catalog () CellTrace SYTOX Green nucleic acid stain CellTrace (AM) AM C3100MP20x50 μg CellTrace calcein probes Jurkat 1.0 μm CellTrace AM CellTrace AM Ex/Em 8 CellTrace calcein blue AM 360/ μg C , CellTrace calcein green AM 494/ μg C , CellTrace calcein red-orange AM 577/ μg C ,
5 CellTracker GST CellTracker CellTracker CMFDA Leptohalysis scottijoan M. Bernhard(Wadsworth Center, New York State Department of Health) 24 CellTracker CellTrace CellTracker 1, 2 1. J Cell Biol 101, 610 (1985); 2. J Cell Biol 103, 2649 (1986). Ex/Em Thiol-reactive long-term cell tracers CellTracker Blue CMAC 353/466 5 mg C , , 14.2 CellTracker Blue CMF2HC 371/464 5 mg C , , 14.2 CellTracker Green CMFDA 492/517 1 mg C , , 15.2, 15.6, 20.2 CellTracker Green CMFDA 492/ μg C , , 15.2, 15.6, 20.2 CellTracker Orange CMTMR 541/565 1 mg C , CellTracker Orange CMRA 548/ μg C , CellTracker Red CMTPX 577/ μg C , CellTracker Violet BMQC 415/ μg C , Amine-reactive long-term cell tracers CFSE (CFDA-SE) 492/ mg C , , 15.4, 20.2 Vybrant CFDA SE Cell Tracer Kit 492/517 1 kit V , , 15.4 CellTrace CFSE Cell Proliferation Kit* 492/517 1 kit C , , 15.4 CellTrace Oregon Green 488 carboxylic acid diacetate, succinimidyl ester 492/ μg C , , 15.4 SNARF -1 carboxylic acid, acetate, succinimidyl ester 576/ μg S , , 15.4, 20.4 CellTrace Far Red DDAO-SE 646/ μg C , * Optimized for tracing via flow cytometry. 210 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
6 Cell Biology Catalog (C18)DiIDiD DiODiODiIDiDVybrant DiIDiODiD CM-DiIDiI DiICM-DiI Vybrant CM-DiINeuroTrace CM-DiI FAST DiI FAST DiO 1 FAST DiI 2 4 FAST DiO 5DiIDiO(C18 : 0)(C18 : 2) DiIDiO50% 1. Trends Neurosci 12, 333 (1989); 2. Neuroreport 8, 3965 (1997); 3. Biophys J 70, 988 (1996); 4. J Cell Biol 134, 1427 (1996); 5. Neuron 17, 91 (1996). NeuroTrace NeuroTrace DiODiICM-DiIDiDDiODiICM-DiIDiD NeuroTrace DiODiIDiD 50 80% DiI(DiIC 18 (3)- )DiD(DiIC 18 (5)- ) Ariel Agmon(West Virginia University) DilC(3)Dil Mount Holyoke Rachel Fink
7 Ex/Em Product Notes DiI and analogs DiIC18(3) DiI 549/565 Red-orange fluorescent lipophilic probe; widely used as a neuronal tracer 100 mg D282 54, , 13.4, 14.4 DiIC18(3) DiI 549/565 Large-crystal form of DiI to facilitate direct application of crystals to membranes 25 mg D , , 14.4 DiIC16(3) 549/565 Shorter-chain DiI analog that may incorporate into membranes more easily than DiI 100 mg D384 54, , 13.4, 14.4 DiIC12(3) 549/565 Shorter-chain DiI analog that may incorporate into membranes more easily than DiI 100 mg D383 54, , 14.4 FAST DiI oil 549/564 Unsaturated DiI analog that reportedly migrates ~50% faster than DiI within membranes 5 mg D , , 14.4 FAST DiI Solid 549/564 Solid form to facilitate direct application of crystals to membranes 5 mg D , , 14.4 Δ9-DiI oil 549/564 Unsaturated DiI analog that may migrate faster than DiI within membranes 25 mg D , , 14.4 CellTracker CM-DiI 553/570 Chloromethylated DiI analog; potentially retained after fixation μg and permeabilization C , , 14.4 CellTracker CM-DiI 553/570 Chloromethylated DiI analog; potentially retained after fixation and permeabilization 1 mg C , , 14.4 SP-DiIC18(3) 556/573 Anionic DiI analog with enhanced solubility in culture medium 5 mg D , , 14.4 DiIC18(3)-DS 555/570 Anionic DiI analog with enhanced solubility in culture medium 5 mg D , , 14.4 DiD and analogs DiIC18(5) oil DiD 644/665 Useful in autofluorescent samples and as a second tracer in combination with DiI 25 mg D307 36, , 14.4 DiIC18(5) DiD 644/663 Solid form to facilitate direct application of crystals to membranes 10 mg D , , 14.4 DiIC18(5)-DS 650/670 Anionic DiD analog with enhanced solubility in culture medium 5 mg D , , 14.4 DiR DiIC18(7) DiR 748/780 Absorption and emission in the near-infrared region, where many tissues are optically transparent 10 mg D , , 14.4 DiO and analogs DiOC18(3) DiO 484/501 Widely used as a second tracer in combination with DiI 100 mg D275 54, , 14.4 DiOC16(3) 484/501 Shorter-chain DiO analog that may incorporate into membranes more easily than DiO 25 mg D , , 14.4 FAST DiO Solid 484/499 Unsaturated DiO analog that reportedly migrates ~50% faster than DiO within membranes 5 mg D , , 14.4 SP-DiOC18(3) 497/513 Anionic DiO analog with enhanced water solubility 5 mg D , , 14.4 DiA and analogs 4-Di-16-ASP DiA 480/540* Useful as a second tracer with DiI 25 mg D , , 14.4 FAST DiA Solid 480/540* Solid form to facilitate direct application of crystals to membranes 5 mg D , , Di-10-ASP 480/540* Shorter-chain DiA analog that may incorporate into membranes more easily than DiA 25 mg D291 46, , 14.4 NeuroTrace tissue labeling pastes NeuroTrace DiI Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 549/565 the tip of a needle 500 mg N , NeuroTrace CM-DiI Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 549/565 the tip of a needle 100 mg N , NeuroTrace DiO Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 484/501 the tip of a needle 500 mg N , NeuroTrace Multicolor Contains 500 mg of DiO, DiI, and DiD labeling pastes for Tissue-Labeling Kit multiple multicolor tracing 1 kit N , * Optimized for tracing via flow cytometry. 212 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
8 Cell Biology Catalog 1 Alexa Fluor (8%)200 mm KClAlexa Fluor - 3 (DAB)(photoconvert) mm 3 (--L-) 4 5 Alexa Fluor 1. Methods Cell Biol 29, 153 (1989); 2. J Neurosci Methods 41, 45 (1992); 3. J Neurosci Methods 33, 207 (1990); 4. Nature 377, 734 (1995); 5. J Neurosci 14, 4338 (1994). 8 CH101 Aplysia californica (J. Neurophysiol 66, 316 (1991)) David Kleinfeld (AT & T Bell Laboratories) Brian Salzberg (University of Pennsylvania School of Medicine) Alexa Fluor 568()Alexa Fluor 488 ()Alexa Fluor 488Alexa Fluor 568 ()3 Michiel van Wyk(University of Queensland, Australia) 213
9 Ex/Em Hydrazide derivatives (fixable) Alexa Fluor /445 5 mg A , , 14.3 Alexa Fluor /517 1 mg A , , 14.3 Alexa Fluor /567 1 mg A20501MP 36, , 14.3 Alexa Fluor /599 1 mg A , , 14.3 Alexa Fluor / μl* A , Alexa Fluor /613 1 mg A , , 14.3 Alexa Fluor / μl* A , Alexa Fluor /643 1 mg A , , 14.3 Alexa Fluor /666 1 mg A , , 14.3 Lucifer yellow CH (lithium salt) 428/ mg L453 19, , 14.3, 16.1 Lucifer yellow CH (lithium salt) 428/ μl* L , Lucifer yellow CH (potassium salt) 428/ mg L , Biocytin derivatives (fixable) Alexa Fluor / μg A , , 14.3 Alexa Fluor / μg A , , 14.3 Alexa Fluor / μg A , , 14.3 Biocytin NA 100 mg B , , 14.3 Other polar tracers (nonfixable) Aminostilbamadine 361/ mg A , hydroxystilbamidine 345/ mg H , , 14.3 Sulforhodamine / mg S359 20, , 16.1 True blue chloride 375/403 5 mg T , * For microinjection. -(-D-1,6-) 1 3 in vivo Vybrant Alexa Fluor 546 4, 5 Net charge FITC Alexa Fluor 488 Alexa Fluor 488 Alexa Fluor B 1. Nature 344, 431 (1990); 2. Science 252, 569 (1991); 3. Development 108, 581 (1990); 4. Brain Res 526, 127 (1990); 5. Brain Res Bull 25, 139 (1990). 214 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
10 Cell Biology Catalog 32 Xenopus laevis31a1 10,000 MW2B1 3C3Texas RED 10,000 MWCascade Blue 10,000 MW1 10 Marie Vodicka Fixable Common Name 3,000 MW dextran conjugates 3000 MW Texas Red Gambusia affinis Bipinnate inclinator muscles Texas Red University of Nebraska Medical CenterE. Rosa-MolinarCreightonBernd Fritzsch Alexa Fluor 488 No 2 mg D , Alexa Fluor 680 No 2 mg D , Amino Yes 100 mg D , Biotin Yes BDA mg D , , 14.5 Cascade Blue Yes 10 mg D , Fluorescein No 10 mg D , , 20.4 Fluorescein Yes 10 mg D , Fluorescein and biotin Yes micro-emerald 5 mg D , , 14.5 Rhodamine Green No 5 mg D , Tetramethylrhodamine No 10 mg D , Tetramethylrhodamine Yes 10 mg D , Tetramethylrhodamine and biotin Yes micro-ruby 5 mg D , , 14.5 Texas Red No 10 mg D , Texas Red Yes 10 mg D , ,000 MW dextran conjugates Alexa Fluor 488 Yes 5 mg D , Alexa Fluor 546 Yes 5 mg D , Alexa Fluor 555 Yes 5 mg D , Alexa Fluor 555 Yes 5 mg D , Alexa Fluor 568 Yes 5 mg D , Alexa Fluor 647 Yes 2 mg D , Alexa Fluor 680 Yes 5 mg D , Amino Yes 1 g D ,
11 Fixable Common Name Biotin Yes BDA-10, mg D , , 14.5 Biotin Yes NeuroTrace BDA-10,000 Neuronal Tracer Kit kit N , BODIPY FL Yes 5 mg D , Cascade Blue Yes 25 mg D , Fluorescein No 25 mg D , , 20.4 Fluorescein Yes fluoro-emerald 25 mg D , Fluorescein and biotin Yes mini-emerald 10 mg D , , 14.5 Lucifer yellow Yes 25 mg D , Oregon Green 488 No 5 mg D , , 20.4 Oregon Green 488 Yes 5 mg D , Rhodamine B No 25 mg D , Rhodamine Green Yes 10 mg D , Tetramethylrhodamine No 25 mg D , Tetramethylrhodamine Yes 25 mg D , Tetramethylrhodamine Yes fluoro-ruby 25 mg D , Tetramethylrhodamine and biotin Yes mini-ruby 10 mg D , , 14.5 Texas Red No 25 mg D , Texas Red Yes 25 mg D , ,000 MW dextran conjugates Amino Yes 1 g D , Fluorescein No 25 mg D , , 20.4 Fluorescein Yes 25 mg D , Tetramethylrhodamine No 25 mg D , Texas Red No 25 mg D , ,000 MW dextran conjugates Amino Yes 1 g D , Biotin Yes BDA-70, mg D , , 14.5 Fluorescein No 25 mg D , , 20.4 Fluorescein Yes 25 mg D , Oregon Green 488 No 5 mg D , , 20.4 Oregon Green 488 Yes 5 mg D , Oregon Green 514 No 5 mg D , , 20.4 Rhodamine B No 25 mg D , Tetramethylrhodamine No 25 mg D , Tetramethylrhodamine Yes 25 mg D , Texas Red No 25 mg D , Texas Red Yes 25 mg D , ,000 MW dextran conjugates Amino Yes 100 mg D , Biotin Yes BDA-500, mg D , , 14.5 Fluorescein Yes 10 mg D , ,000,000 MW dextran conjugates Fluorescein Yes 10 mg D , Tetramethylrhodamine Yes 10 mg D , Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
12 Cell Biology Catalog BCTBCTB BAAlexa Fluor Bovine serum albumin (BSA) conjugates Alexa Fluor mg A , Alexa Fluor mg A , Alexa Fluor mg A , Alexa Fluor mg A , Alexa Fluor mg A , BODIPY FL 5 mg A , DNP 5 mg A , Fluorescein 5 mg A , Tetramethylrhodamine 5 mg A , Texas Red 5 mg A , Ovalbumin conjugates Alexa Fluor mg O , Alexa Fluor mg O , Alexa Fluor mg O , Alexa Fluor mg O , Fluorescein 5 mg O , Texas Red 5 mg O , Cholera toxin subunit B conjugates Alexa Fluor μg C , , 14.7, μg C , , 14.7, 16.1 Alexa Fluor μg C , , 14.7, μg C , , 14.7, 16.1 Alexa Fluor μg C , , 14.7, μg C , , 14.7, 16.1 Alexa Fluor μg C , , 14.7, 16.1 Biotin-XX 100 μg C , , 7.7, 14.7, 16.1 Horseradish peroxidase 100 μg C , , 14.7, 16.1 Other protein conjugates Protein Histone H1 (calf thymus) Alexa Fluor mg H , , 16.1 Parvalbumin (codfish) Alexa Fluor mg P , Trypsin inhibitor (soybean) Alexa Fluor mg T , , 16.1 SAIVI injectable contrast reagents Bovine serum albumin Alexa Fluor ml S , Human serum transferrin Alexa Fluor ml S , Alexa Fluor ml S ,
13 NeuroTrace Franz Nissl100 -O O 5 FluoroMyelin NeuroTrace 530/615 DAPI DAPIBrainStain MBPLoyezSchmued's 20 HuC/HuD embryonic lethal abnormal visual(elav) RNAHuDHuCHel-N1 HuR FluoroMyelin Green FluoroMyelin RedNeuroTrace DAPI NeuroTrace fluorescent Nissl stains Color Ex/Em Blue 435/455 1 ml N , , 12.5, 14.3 Green 500/525 1 ml N , , 12.5, 14.3 Yellow 515/535 1 ml N , , 12.5, 14.3 Red 530/615 1 ml N , , 12.5, 14.3 Deep red 640/660 1 ml N , , 12.5, 14.3 FluoroMyelin myelin stains Green 479/598 1 ml F , Red 558/654 1 ml F , BrainStain Imaging Kit Contents Contains FluoroMyelin Green, NeuroTrace 530/615 red Nissl stain, and DAPI B , Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s)
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