,, 1 3,,, 70 90, 2 3, 2 4, 1 2,,,, 2,, M. sympodialis,, IgE, Kieffer 55, 19, 19 IgE,, IgE RAST 2/3. RAST, 3 IgE 1,000 3, M. furfur/ Pit
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1 Jpn. J. Med. Mycol. Vol. 48, , 2007 ISSN ,,,,,,, Malassezia furfur, Malassezia pachydermatis, Malassezia sympodialis, Malassezia globosa, Malassezia obtusa, Malassezia restricta Malassezia slooffiae, Malassezia dermatis, Malassezia yamatoensis, Malassezia japonica, Malassezia nana ,,,, 1, 20 30,,,, 2 50,,,, PAS, 5,,.,, 3,,,,.,,,,,,,,, 2,, Hemacolor,, PAS, 3,,,,,,
2 ,, 1 3,,, 70 90, 2 3, 2 4, 1 2,,,, 2,, M. sympodialis,, IgE, Kieffer 55, 19, 19 IgE,, IgE RAST 2/3. RAST, 3 IgE 1,000 3, M. furfur/ Pityrosporum orbiculare Candida albicans IgE,.
3 Jpn. J. Med. Mycol. Vol. 48 No. 4, Malassezia I Malassezia, Malassezia, 10, Malassezia, II Malassezia pachydermatis LNA, Fig m,,, Fig. 2 Malassezia globosa, III Malassezia, 1846 Eichstedt 1853 Robin Microsporon furfur, 1889 Baillon Malassezia., 1904 Sabouraud Pityrosporum, Malassezia, Pityrosporum Malassezia Pityrosporum, Malassezia, Malassezia furfur heterogeneity M. furfur, Gue ho 1 DNA- DNA rrna, 5,, 4, 2002, 2003, 2004 Malassezia dermatis 2, Malassezia japonica 3 Malassezia yamatoensis , Hirai 5 Malassezia nana 11 Fig , Malassezia Fig. 1 mlna Malassezia 32 C 5 Fig. 2 Malassezia 10 m
4 Fig. 3 Malassezia D1/D2 26S rdna 600 bp NJ M. furfur , 3 4 Table 1. Malassezia
5 Jpn. J. Med. Mycol. Vol. 48 No. 4, IV Malassezia, Cryptococcus Trichosporon, class hymenomycetes, Malassezia class ustilaginomycetes Fig. 3, rdna rrna ITS Internal Transcribed Spacer D1/D2 26S rdna 99, Malassezia ITS, D1/D2 26S rdna Fig. 4 V Malassezia, Tween, Dixon LNA CBS Centraalbureau voor Schimmelcultures modified LNA, Malassezia 6. Malassezia, API Tween, Table 1. Malassezia VI 1. Malassezia,, Malassezia 1996, M. furfur, Malassezia,,, 7. OpSite, DNA rrna IGS intergenic spacer ITS PCR, 30 PCR Fig. 4 Malassezia rrna ITS, internal transcribed spacer; IGS, intergenic spacer., bp, M. globosa CBS 7966 Fig. 5 Malassezia PCR primer nested PCR Fig. 6 Malassezia M. globosa M. restricta M. g, M. globosa ; M. r, M. restricta.
6 VII, KTZ ITZ Malassezia g/ml 12, , 14. KTZ ITZ, Malassezia MIC, Fig. 7 Malassezia globosa IGS1 DNA AD, HS,,,, M. globosa M. restricta,, Fig , real-time PCR, M. globosa, M. restricta Fig. 6, Malassezia IgE, 2. M. globosa M. restricta rrna IGS, CT n, GT n SSR, short sequence repeat,, Fig. 7 10, Gue ho E, Midgley G, Guillot J: Antonie Van Leeuwenhoek 69: , Sugita T, Takashima M, Shinoda T, Suto H, Unno T, Tsuboi R, Ogawa H, Nishikawa A: J Clin Microbiol 40: , Sugita T, Takashima M, Kodama M, Tsuboi R, Nishikawa A: J Clin Microbiol 41: , Sugita T, Tajima M, Takashima M, Amaya M, Saito M, Tsuboi R, Nishikawa A: Microbiol Immunol 48: , Hirai A, Kano R, Makimura K, Duarte ER, Hamdan JS, Lachance MA, Yamaguchi H, Hasegawa A: Int J Syst Evol Microbiol 54: , Kaneko T, Makimura K, Onozaki M, Ueda K, Yamada Y, Nishiyama Y, Yamaguchi H: Med Mycol 43: , Sugita T, Suto H, Unno T, Tsuboi R, Ogawa H, Shinoda T, Nishikawa A: J Clin Microbiol 39: , Morishita N, Sei Y, Sugita T: Mycopathologia 161: 61 65, Tajima M, Sugita T, Nishikawa A, Tsuboi R: submitted 10 Sugita T, Kodama M, Saito M, Ito T, Kato Y, Tsuboi R, Nishikawa A: J Clin Microbiol 41: , Sugita T, Tajima M, Amaya M, Tsuboi R, Nishikawa A: Microbiol Immunol 48: , Sugita T, Tajima M, Ito T, Saito M, Tsuboi R, Nishikawa A: J Clin Microbiol 43: , Sugita T, Tsubuku H, Tajima M, Tsuboi R, Nishikawa A: Microbiol Immunol 50: , Sugita T, Tajima M, Tsubuku H, Tsuboi R, Nishikawa A: Antimicrob Agents Chemother 50: , 2006.
7 Jpn. J. Med. Mycol. Vol. 48 No. 4, ,,,,,,,,, 1,3 - -D-. 1,3 - -D-, 1,3 - -D-.,,,,,,, B,, 3 5,,,,,, 2002, 2005, 2006 B,, 60 70, 30 40,,,
8 ,,,,,,, 1.,,,,,,,, 1 2 MHC II 2 2.,,, HIV,,, 1, 2., 2.,,,,,.,,,,, 3.,. 4.,,,,,,,,,, MHC II 4., 4., CD4+T MHC
9 Jpn. J. Med. Mycol. Vol. 48 No. 4, II,, CD4+T HIV, HIV, gp120 CD4, CD4, CD4+T. MHC II, Ia L3/T4+, CD4+T,, 1., 4., 1996, Highly Active Antiretroviral Therapy HAART CD4+ 1., HAART,,, HIV, Th-1,. 5., 2).,,, 6., 2 lobular consolidation discrete nodule 2,,,,,,,,, 1,500/cm,, 2.,, 7. 1 Shibuya K, Hirata A, Omuta J, Sugamata M, Katori S, Saito N, Murata N, Morita A, Takahashi K, Hasegawa C, Mitsuda A, Hatori T, Nonaka H: Granuloma and cryptococcosis. J Infect Chemother 11: , Shibuya K, Ando T, Hasegawa C, Wakayama M, Hamatani S, Hatori T, Nagayama T, Nonaka H: Pathophysiology of pulmonary aspergillosis. J Infect Chemother 10: , Simon MR, Houser WL, Smith KA, Long PM: Esophageal candidiasis as a complication of inhaled corticosteroids. Ann Allergy Asthma Immunol 79: , Shibuya K, Coulson WF, Wolman JS, Wakayama M, Ando T, Oharaseki T, Takahashi K, Naoe S: Histopathology of cryptococcosis and other fungal infections in patients with acquired immunodeficiency syndrome. Int J Infect Dis 5: 78 85, Paris S, Wysong D, Debeaupuis J, Shibuya K, Philippe B, Diamondo R, Latge J: Catalases of Aspergillus fumigatus. Infect Immun 71: , Aikawa N, Kohno S, Shibuya K, Takesue Y, Maesawa S, Yoshida M, Tokimatsu I: Current status of diagnosis and tretment of invasive fungal infections in Japan: the influence of the new Japanese guidelines. J Infect Chemother 11: , Shibuya K, Paris S, Ando T, Nakayama H, Hatori T, Latge J: Catalases of Aspergillus fumigatus and inflammation in aspergillosis. Jpn J Med Mycol 47: , 2006.
10 Actinomycetes,, Bergey s Manual of Determinative Bacteriology,,, Actinomyces, Nocardia 1. Actinomyces actinomycosis, Nocardia nocardiosis, Nocardia, Rhodococcus, Tsukamurella, Gordonia 2,, Mycobacterium,, Corynebacterium, Mycobacterium, Nocardia,,,, RNA,, 1, Mycobacterium, Nocardia, Rhodococcus, Gordonia Tsukamurella Actinomadura, Rothia Nocardiopsis, Nocardia, Nocardia,,,., Nocardia Actinomyces 2,., Nocardia, Nocardia,,, 1 2.,, Nocardia, Nocardia, Ziehl-Neelsen, 0.5 2,,,, Nocardia,,,,, Nocardia Nocardia, 16S-rRNA,, Nocardia, Nocardia,, 60, ,., Nocardia farcinica Ishikawa et al, PNAS, ,
11 Jpn. J. Med. Mycol. Vol. 48 No. 4, Knac N. paucivorans DSM T (AF430041) 998 N. brevicatena DSM T (AF430040) N. gamkensis CZH20 T (AB187522) N. exalbida IFM 0803 T (AB187522) 99 N. pseudobrasiliensis DSM T (AF430042) N. crassostreae JCM T (AF430049) N. uniformis DSM T (AF430044) 142 N. otitidiscaviarum ATCC T (X80599) 167 N. seriolae JCM 3360 T (Z36925) N. niigatensis IFM T (AB092563) N. neocaledoniensis SBHR OA6 T (AY282603) N. asteroides ATCC T (AY505492) N. abscessus JCM T (AF218292) N. cyriacigeorgica DSM T (AF430027) N. flavorosea JCM 3332T (AF430048) N. testacea IFM 0937 T (AB192415) N. sienata IFM T (AB121770) N. pigrifrangens 7031 T (AF219974) N. carnea DSM T (AF430035) N. vinacea MK F1 T (AB024312) N. pseudovaccinii DSM T (AF430046) 722 N. anaemiae IFM 0323 T (AB162801) N. miyunensis 117 T (AY639901) 460 N. nova N. jiangxiensis JCM T (AY639902) 6044T (AF430028) N. vermiculata IFM 0391 T (AB126873) 416 N. N. elegans vaccinii IMMIB DSM T (AF430045) N-402T (AJ854057) N. yamanashiensis IFM 0265 T (AB092561) N. inohanensis IFM 0092 T (AB092560) N. jejuensis N3-2 T (AY964666) N. concava IFM 0354 T (AB126880) N. cerradoensis Y9 T (AF060790) 940 N. N. aobensis veterana IFM DSM 0372T (AB126876) T (AF430055) N. kruczakiae MB2876 T (AY441974) 85 N. africana DSM T (AY089701) N.terpenica IFM 0706 T (AB201298) N. mexicana DSM T (AY555577) N. xishanensis CGMCC T (AY333115) N. polyresistens YIM T (AY626158) N. lijiangensis YIM T (AY ) N. thailandica IFM T (AB126874) N. caishijiensis F829 T (AF459443) N. ignorata DSM T (AY191254) N. fluminea DSM T (AF430053) N. salmonicida JCM4826 T (Z46750) N. soli DSM T (AF430051) N. cummidelens DSM T (AF430052) N. ninae DSM T (DQ235687) N. alba YIM T (AY222321) N. transvalensis DSM T (AF430047) N. harenae WS-26 T (DQ ) N. takedensis MS1-3 T (AB158277) N. tenerifensis GW T (AJ556157) N. brasiliensis ATCC T (X80591) N. puris IMMIB R-145 T (AJ508748) N. shimofusensis IFM T (AB108775) N. higoensis IFM T (AB108778) N. farcinica ATCC 3318 T (X80595) N. asiatica IFM 0245 T (AB092566) N. arthritidis IFM T (AB108781) N. pneumoniae IFM 0784 T (AB108780) N. beijingensis AS T (AF154129) N. araoensis IFM 0575 T (AB108779) R. opacus DSM T (X80630) 1. Nocardia
12 Nocardia Nocardia, Actinomadura 4, Nocaridipsis Rothia 1 Family Actinomycetaceae Rainey et al 1997, Actinomyces, Mobiluncus, Archanobacterium Actinobaculum, Actinomyces Actiomyces,,,,, sulfur granule, Actinomyces israelii,,. 2,,, 16S-rRNA,, Actinomyces israelii, Propionibacterium propionicus,,, 1 Iida S, Kageyama A, Yazawa K, Uchiyama N, Toyohara T, Chohnabayashi N, Suzuki S, Nomura F, Kroppenstedt RM, Mikami Y: Nocardia exalbida sp. nov., isolated from Japanese patients with nocardiosis. Int J Syst Evol Microbiol 56: , Kageyama A, Iida S, Yazawa K, Kudo T, Suzuki S, Koga T, Saito H, Inagawa H, Wada A, Kroppenstedt RM, Mikami Y: Gordonia araii sp. nov. and Gordonia effusa sp. nov. isolated from patients in Japan. Int J Syst Evol Microbiol 56: , Ishikawa J, Yamashita A, Mikami Y, Hoshino Y, Kurita H, Hotta K, Shiba T, Hattori M: The complete genome sequence of Nocardia farcinica IFM Proc Natl Acad Sci USA 101: , Hanafy A, Ito J, Iida S, Kang Y, Kogure T, Yazawa K, Takashi Yaguchi T, Mikami Y: Majority of Actinomadura clinical isolates from sputa or bronchoalveolar lavage fluid in Japan belongs to the cluster of Actinomadura cremea and Actinomadura nitritigenes, and the description of Actinomadura chibensis sp. nov. Mycopathologia 164: , 2006.
13 Jpn. J. Med. Mycol. Vol. 48 No. 4, I Sporothrix schenckii,,., S. schenckii,,,,,,.,,,,,,.,,,,,,,, ,,,,,, PAS,,, asteroid body, KOH, PAS,,,, 25 C, 5,, DNA,,,,,,,,,, ,,, S. schenckii 39 C, 1,,,,. II,,,
14 ,,,, Fonsecaea pedrosoi, Exophiala jeanselmei, E. dermatitidis, Phialophora verrucoza, Cladosporium trichoides,,,,,,,,,,,, host, E. jeanselmei KOH, sclerotic cell HE sclerotic cell. F. pedrosoi Cladosporium, Rhinocladiella, Phialophora,,,,.,,, mg/ 60,,,,,
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MALDI-TOF MS 微生物迅速同定スペクトル ライブラリーの提供 MALDI-TOF MS 微生物迅速同定スペクトル ライブラリーの提供 NITE では MALDI-TOF MS を用いたより確実な微生物迅速同定法を開発しています マトリックス支援レーザー脱離イオン化飛行時間型質量分析装置 (MALDI-TOF MS) を用いた微生物の迅速同定技術は 遺伝子解析に比べて少量のサンプルで 迅速かつ簡便で
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