Table l. Composition of artificial medium solution used for eggs from raibow trout. Constitueflt Concentralion (mmole)
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1 Journal of the Tokyo UDiversity of Fisheries, Vol. 79, No. l, p- L2l-126, March 1992 The cryopreservation of orsanic tissues in living condition has been studied by maoy researcbers. However, no treattuent wich has beetr proposed in available literature is successeful to preserve fish eggs alive. In order to perfolm the cryopreservatioo of 6sh eggs, it is oecessary to reveal the oplimum concentrations of cryoprot ctabts and their efleclive applicatioo procedur s. Dimethylsulphoxide (DMSO), a vely popular and effective cryoprotectant, was chosed id this study. The mortality of rainbow trout eggs (eyed stage) which were imeerscd id solutions of valious DMSO codcenlratiods at looc was.elated to the time of exposure, The concentration of DMSO io the esgs was calculated for various permeability co. efficient of DMSO thlough the egg membratre. The.esults suggested that the toleraoce of the eggs to DMSO depetrded upon odly its coocentlation io the eggs and the mortality of eggs became significadt whed the codcentmtiod of DMSO increased Dear [mol/l]. The appropliate permeability coemcient explaining the expelimental rcsults was 2.0x10{-8.0x10-6[cm/sec] in this study.
2 Table l. Composition of artificial medium solution used for eggs from raibow trout. Constitueflt Concentralion (mmole)
3 a0 100 Tlm.(l,lln.) Thc chatrge of mo.tality at various codc.nrado$ of DMSO.
4 t-4. oxto-t(cm,/r.c)
5 C-r-4 (mo t/t)
6
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