Key words:fatty acid,plant oil,staphylococcus aureus,skin care, atopic dermatitis
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1 Key words:fatty acid,plant oil,staphylococcus aureus,skin care, atopic dermatitis
2 growth was monitored at 660 nm with a biophotorecorder. Table 1.Relative inhibitory effects of fatty acids,plant oils, squalane and liquid paraffin on growth of Staphylococcus aureus FDA 209 P Minimal effective concentration of samples which showed an inhibitory effect on the growth of S.aureus FDA 209 P. S.aureus FDA 209 P was inoculated into free BHI broth medium and broth media containing 40ƒÊg of one of the sample materials per ml.delays in growth time were calculated from the growth curve. NI:Not inhibitory at the concentrations tested.
3 (A) (B) (C) (D) Fig.2.Inhibitory effects of plant oils on the growth of Staphylococcus aureus FDA 209 P. (A),camellia oil;(b),purified camellia oil;(c),olive oil;(d), jojoba oil. S.aureus FDA 209 P was inoculated into BHI broth medium containing no oil(0)or broth media containing 2.5,5,10,20,40 or 80(+,,, œ,, )ƒêg of oil per ml.cell growth was monitored at 660nm with a biophotorecorder.
4 Fig.3.Inhibitory effects of squalane and liquid paraffin on the growth of Staphylococcus aureus FDA 209 P. S.aureus FDA 209 P was inoculated into BHI broth medium containing no oil( )or broth media containing 10,20,40 or 80(, œ,, ),ug of oil per ml.(a),squalane;(b),liquid paraffin.cell growth was monitored at 660 nm with a biophotorecorder.
5 Fig.4 Relative inhibitory effects of fatty acids and oils on the growth of Staphylococcus aureus FDA 209 P. Fatty acids and oils at concentrations ranging from 1.25 to 80ug/ml were used to determine the ID 50 for fatty acids and oils.symbols:a,linoleic acid;,oleic acid;,camellia oil;,purified camellia oil; œ,olive oil. 10) Kabara J J, Swieczkowski D M, Conley A J, Truant J P: Fatty acids and derivates as antimicrobial agents. Antimicrob Agents Chemother 2: 23 ` 28, ) Willet N P, Morse G E: Long-chain fatty acid inhibition of Streptococcus agalactiae in a chemically defined medium. J Bacteriol 91: 2245 `2250, ) Coonrod J D: Role of surfactant free fatty acids in antimicrobial defenses. Eur J Respir Dis 153: 209 `214, ) Japan Society of Chemotherapy, Chemotherapy 29: 76-79, ) Stillman M A, Maibach H I, Shalita A R: Relative irritancy of free fatty acids of different chan lenght, Contact Dermatitis 1: 65-69, 1975
6 VOL.44 NO.10 Inhibitory effects of fatty acids purified camellia oil and olive oil on the growth of Staphylococcus aureus Taketoshi Arai,Hajime Hamashima and Masanori Sasatsu Department of Microbiology,Showa College of Pharmaceutical Sciences Machida,Tokyo 194,Japan Linoleic acid,oleic acid,camellia oil,purified camellia oil,olive oil,jojoba oil,squalane and liquid paraffin were examined for inhibitory activity against Staphylococcus aureus FDA 209 P. Standard solutions of oils at 80ug/ml were serially diluted two-fold with additional broth.an overnight culture was added to each dilution to give approximately 1.0 ~107 colony-forming units per ml(cfulml).the inhibitory activity of samples on growth was monitored turbidimetrically.the results showed that linoleic acid,oleic acid and camellia oil had strong inhibitory activity against S.aureus FDA 209 P.Both purified camellia oil and olive oil showed relatively low inhibitory activity against S.aureus FDA 209 P.The 50% inhibtory dose(id 50)of purified camellia oil was compared with that of other fatty acids and oils.a comparison of the ID 50 of purified camellia oil with those of other fatty acids and oils revealed that purified camellia oil exerted stronger inhibitory activity than that of olive oil.jojoba oil,squalane and liquid paraffin showed. no inhibitory activity against S.aureus FDA 209 P at the concentrations tested.the results suggest the purified camellia oil and olive oil are potentially useful skin care products for skin affected by atopic dermatitis.
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32 2008 pp. 93 106 1 Received October 30, 2008 The purpose of this study is to examine the effects of aerobics training class on weight loss for female students in HOKURIKU UNIVERSITY. Seventy four female
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11-19 2012 1 2 3 30 2 Key words acupuncture insulated needle cervical sympathetick trunk thermography blood flow of the nasal skin Received September 12, 2011; Accepted November 1, 2011 I 1 2 1954 3 564-0034
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