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- ありさ いざわ
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1
2 COLAK Mehmed
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9 GH IGF-1
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13 GH GH
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21 Kawashima et al., J Reprod Dev, 52 (2006)
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26 1 Kawashima et al., J Dairy Sci, 90 (2007)
27 Kawashima et al., J Dairy Sci, 90 (2007)
28 Kawashima et al., J Dairy Sci, 90 (2007)
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33 P<0.001 vs Kawashima et al., Reproduction, 133 (2007)
34 P<0.05 P< P<0.01 P< Kawashima et al., Reproduction, 133 (2007)
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37 P< vs P< vs Kawashima et al., J Reprod Dev, 53 (2007)
38 P< vs. Kawashima et al., J Reprod Dev, 53 (2007)
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40 IGF (Insulin-like growth factor), IGF-I IGF-II (Type I II) (1-6)
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42
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44 E2
45 Kawashima et al., Reproduction, 133 (2007)
46 P< Kawashima et al., Reproduction, 133 (2007)
47 0.8 n=5 0.8 n= E Kawashima et al., Reproduction, 133 (2007)
48
49
50 Exp.1. ; (1) 1 1 cm 2 mm Aloka SSD cm 11
51 Exp.2. LH ; (1) GnRH 1 cm Pre GnRH GnRH 0.5 h 1 h 2 h 6 h 12 h 24 h 30 h 48 h Acosta et al., Reproduction, 125 (2003)
52 Exp.2. LH ; (1) 1 cm -30 h -24 h -18 h -6 h 0 h LH 6 h 12 h 24 h Acosta et al., Reproduction, 125 (2003)
53 Exp.2. LH ; (1) 42
54 GH
55 LH (E2) FSH LH FSH LH
56 LH LH P4 P4 P4 P4
57
58 FSH LH E2 P4 GH IGF-1?? 5
59 LH >8 mm 1 P4 2 PGF2α 2 42 h 1 LH P4 P4 39
60 PGF2α 42h 3 6
61 VS. (P4 E2) LH GH IGF
62 12 mean SEM F1 F2 F3 F4 12 mean SEM (mm) (mm) Day 1 F1 F2, F3, F4 (P<0.05) Day 1 F1, F2 F3, F4 (P<0.05) 41
63 1 P4 (ng/ml) P4 mean SEM *: P<0.05 **: P<0.01 ***: P<0.001 Between two groups * * * * ** *** E2 (pg/ml) E2 mean SEM *: P<0.05 Between two groups * * * * FSH (ng/ml) FSH mean SEM 2 42
64 LH 1 F1 F1 F2 F3 F2 GH 51
65 mrna expression (x10 4 ) mean SEM a,b: P<0.05 a LH x b y c z mrna expresion (x10 4 ) mean SEM a,b: P<0.05 x,y: P<0.05 a x 0 GH b y b xy
66 1 IGF mean SEM a,b: P<0.05 a 1.5 mean SEM IGF-1 (ng/ml) b b b ab ab ab ab Insulin (ng/ml) Day 2 GH???? 47
67 GH LH IGF-1 2 F1 F2 F1 F2 E2 LH P4 P4 LH 2 P4 E2 LH GH GH IGF
68 : : EIA : P4 GH IGF-1
69 P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) ab a ab ab ab ab ab b a cd bc ab bcd cd cd d a ab bc ab cd de e de P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) a b b b b b ab 4 ab 3 abc 2 1 d cd b b a abc bcd Kawashima et al., J Reprod Dev, 53 (2007)
70 E2 LH GH P4?? IGF-1
71 GH LH E2 P4?? LH GH IGF-1 5
72
73 Mehmet ÇOLAK
74
75 Growth hormone GH Insulin? Steroids IGF-1 Insulin-like growth factor-1
76 Control (n=3) P4 (n=4) 2 CIDR BIOPSY SLAUGHTERHOUSE EB inj IM (1 mg) BIOPSY SLAUGHTERHOUSE E2 (n=3) BIOPSY SLAUGHTERHOUSE Serial Blood Collection 15 min, 16 h Daily Blood Collection
77
78 Analysis of Plasma E2, P4, GH, IGF-I and Insulin Hormone analyses were performed by EIA Analysis of GHR, IGFR-I and IR genes Quantitative PCR method using BioRad
79 P4 (ng/ml) *** *** *** *** *** *** *** Mean S.E.M. ***:P< E2 (pg/ml) *** *** DAY
80 Control GH (ng/ml) P4 group E2 group Time (h)
81 (ng/ml/min) a b c Mean S.E.M. a, b, c: P<0.05 (ng/ml) a b a PULSAR program 0 (16 h) b a a Control E2 P4
82 140 Control P4 E2 * Mean S.E.M. *:P< IGF-I (ng/ml) Before After Before After Before After
83 Total GH-R P=0.055 mrna ( 10-7 ) Insulin-R mrna IGF-R1 mrna ( 10-6 ) b a a Control E2 P4 b a a Mean S.E.M. a, b: P<0.05
84 Growth hormone GH E2 IGF-I GHR IGFR-I IR
85 Estradiol+Progesterone Eimeria bovis * * * * * * * * Percentage Feed Intake Day Heath et al, Am J Vet Res (1997)
86 Estrogen+Proegesterone, but not GH *a a *a *a *a Weight Gain (kg) Con EP GH PF C EP\C GH\C * a Heath et al, Am J Vet Res (1997)
87
88 350 Duration (min) * * * *,* * C: control E: endotoxin S: Synovex S (P/E) C/E Saliva S/E Saliva C/E Pant S/E Pant C/E Cough S/E Cough McMahon et al, J. Endocrinol., (1998)
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93 Growth hormone GH E2 IGF-I GHR
94 Growth hormone GH E2 P4 GHR IGF-I GHIGF-I E2/P4
95 E/P
96 IGF IGF-1 IGF-1
97
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100 Thank you for your attention!!
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(1.2) T D = 0 T = D = 30 kn 1.2 (1.4) 2F W = 0 F = W/2 = 300 kn/2 = 150 kn 1.3 (1.9) R = W 1 + W 2 = = 1100 N. (1.9) W 2 b W 1 a = 0
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19 1 19 19 3 8 1 19 1 61 2 479 1965 64 1237 148 1272 58 183 X 1 X 2 12 2 15 A B 5 18 B 29 X 1 12 10 31 A 1 58 Y B 14 1 25 3 31 1 5 5 15 Y B 1 232 Y B 1 4235 14 11 8 5350 2409 X 1 15 10 10 B Y Y 2 X 1 X
10 2 2 10 6.5 78 1 65 / 30 / - 2 -
- 1 - 10 2 2 10 6.5 78 1 65 / 30 / - 2 - 3 3 30 8 4 8 6 11 14 45 14 7 8 1-3 - 4 1 () 20 4 9 4 9 3 9 4 PR 4 3-4 - - 5 - PR 15 4 PR 7 8 4 9 10-6 - 9 10 9 10 4 9 10 3 9 10 9 9 9 10 PR 1-7 - PR - 8 - 30 100-9
A comparison of abdominal versus vaginal hysterectomy for leiomyoma and adenomyosis Kenji ARAHORI, Hisasi KATAYAMA, Suminori NIOKA Department of Obstetrics and Gnecology, National Maizuru Hospital,Kyoto,
56cm 1 15 1960 2 8 2 2 1 2008 1992 2 1992 2 3562mm 3773mm 2 1980 1991 2008 2007 2003 5 2 3 2003 2005 2008 2010 2005 2008 2012 2010 2012 4 7 4 5 2 1975 1994 8 2008 NPO 2 2010 3 2013 2016 3 2008 2009 14
エッセー
5.13................................... 3 5.14........................................ 9 5.15.................................. 12 5.16................................ 15 5.17..................... 18 5.18...................................
02
Q A Ax Pb Ni Cd Hg Al Q A Q A Q A 1 2 3 2 0 1 2 3 4 5 6 7 0 1 2 3 4 5 6 7 8 0 0 1 2 3 4 1 0 1 2 Q A ng/ml 4500 D H E A 4000 3500 3000 2500 2000 DHEA-S - S 1500 1000 500 0 10 15 20 25 30 35 40 45
...J......1803.QX
5 7 9 11 13 15 17 19 21 23 45-1111 48-2314 1 I II 100,000 80,000 60,000 40,000 20,000 0 272,437 80,348 82,207 81,393 82,293 83,696 84,028 82,232 248,983 80,411 4,615 4,757 248,434 248,688 76,708 6,299