培養歯髄細胞を骨欠損部に自家移植することによる骨治 Title 癒に与える影響 Author(s) 松岡, 海地 Journal, (): - URL http://hdl.handle.net/10130/28 Right Posted at the Institutional Resources for Unique Colle Available from http://ir.tdc.ac.jp/

2 P6 P P8 P9 P11 ODF-RANK P16

P23 Tissue Engineering P24 3

P28 P29 P30 P32 P33 P34 P36 4

5

GTR 6

(1)(2)(3) (1)(2)(3)(4)(5) 7

(1)(2)(3)(4)(5) (1)(2)(3)(4)(5) (1)(6)(7) 8

X X (1) (8)(9) 9

(8)(9)(10) 10

11 (8) (11) H-thymidine H-thymidine H-thymidine H-thymidine (8)(10)(11)(12)(13) Cbfa-1 SOX9 MyoD PPAR

Cbfa-1 (7) (7) (undifferentiated mesenchymal cell) (7) 12

13 (7) D in vitro (14) Bone morphogenetic protein(bmp)

basic fibroblast growth factor(bfgf) PGs, transforming growth factor- (TGF-), platelet-derived growth factor(pdgf), insulin-like growth factor(igf) TGF- IGF BMP, PGs, IGF ALP Cbfa1 Cbfa1 BMP BMPs, TGF-, IGF-I lining cell (15) K L 14

(16) Rodan Martin D RGD H H ph4 K K (16) ODF-RANK ODF-RANK ODF-RANK M-CSF M-CSF (c-fms) D, PGE2, PTH, IL-1, IL-11 ODF ODF RANK (16) 15

lining cell PTH IL-11 lining cell ODF ODF-RANK lining cell (16) ODF-RANK BMP,IGF TGF- (16) 16

(17) mm (17) (17) 17

(17) (17) (dental-pulp complex) m 18

(hydrodynamic theory) (17) odontoblast layer cell-free zone Weil s layer cell-rich zone Raschkow s nerve plexus µ µ µ 19

(17) subodontoblast plexus Ad Aß Ad Aß 17 dentin bridge 20

21 (17) A B (8) (8)(9) (Odontoblast differetiation)

TGF- BMP2 IGF (13) (13) 22

NCPs NPCs DPPAG1 Dmp1 DSP DPP DPP DPP DPP AG1 DSP DSPmRNA BPS NCPs OPN (13) Snell major histocompatibility complex;mhc (18)(19)(20)(21)(22) F1 23

MHC first set rejection second set rejection MHC (23) Tissue Engineering embryonic stem celles somatic adult stem cell ES (24) 1990 adult stem cell G0 24

10 TA transit amplifying cell (24) (embryonic stem cell)es ES ES ES teratoma ES ES ES ES ES (therapeutic cloning) (24) (24) TGF-ßFGF 25

WNT (24) Tissue Engineering FGF BMP,TGF-ß,IGF-1 BMP, TGF-ß Tissue Engineering 26

27

GTR(25) (26) (25) (27) 17 BMPs, TGF-ß (24) 28

g Sprague-Dawley ml/bw mm Dish(Falcon, USA) a-minimal essential medium (a-mem,gibco, USA) Fetal bovine serum(fbs, GIBCO, USA)ascorbic acid (50 µl/ml, Wako, Japan) gentamicin (SIGMA, USA) mm SM01060 a b c 29

H-E Proliferating Cell Nuclear Antigen (PCNA) VENTANA Ventana HX System Discovery PCNADAKO H-E 3 (Fig.1, a, b, c) 7 (Fig.2, a, b) 14 (Fig.3, a, b) 3 (Fig.1, d, e, f) 7 (Fig.2, c, d) 14 (Fig.3, c, d) 30

HE 3 7 14 PCNA 3 (Fig.4, a, b, c) 7 (Fig.5, a, b, c) 14 (Fig.6, a, b, c) 3 (Fig.4, d, e, f) 7 (Fig.5, d, e, f) 14 Fig.6, d, e, f PCNA 3 7 14 31

BMP, IGF TGF-ß 16BMP TGF-ß IGF PCNA BMP TGF-ß (24) 32

33

1. P20~35 2.. In: 1999b:62-63 3. 1999 70-75 4. Bryant WM. Wound healing.clin Symp.1977;29 (3):1-36 5. Hupp JR. wound repair.in: Peterson LJ, Ellis?EHupp JR Tucker MR.eds:Contemporary Oral and Maxillofacial Surgery. St. Louis : osby.1998:57-68 P75P~79 P71 P11211~6 P162P~172 10. Ten CateA.ROral Histology. Development, Structure, and Function (2 nd ed.).mosby, St.Louis,1985,14618~2 H-thymidine autoradiography 12. The Quintessence,5(1):143~0,1986 13. Trowbrige,H.O.: Pulp histology and physiology. In Pathways of the Pulp (3 rd ed.).s.cohen R.C.Burns (eds), Mosby,St.Louis,1984,3233~78 14. Ten Cate P167~169 WILLIAM T.BUTLERandHELENA RITCHIE 173 16. P11,12 P183~188 P267~274 P14~17 20. Gill Tj,Kunz HW,MisraDN,et al:the major histocompatibility complex of rat,transplantation43:773-785,1987 21. Stepkowski SM,Ito T:Frequency of alloantigen-specfic T cytotoxic cell in high-and low-responder recipients of class? MHC-disparate heart allografts,transplantation 50:112-119,1990 22. Yamamoto S,Ito,Nakata S,et al:the rejection mechanism of rat pancreaticoduodenal allografts with a class? MHCdisparity,Transplantation 57:1217-1222,1994 34

P451~454 P18P26 25. Smith AJ, Lesot H (2001). Induction and regulation of crown dentinogenesis: embryonic events as a template for dental tissue repair? Cri Rev Oral Biol Med 12:425-437. 26. Melcher.AH: On the repair potential of periodontal tissues. J. Periodontol. 47:256~260.1976 27. Hammarstrom,L.,Heiji,LGestrelius,S.: Periodontal regeneration in a buccal dehiscence model in monkeys after application of enamel matrix proteins.j.clin.periodontol.,24:669~677,1997. 28. Dahlin,C.,Linde,A.,Gottlow J.,Nyman,S.:Healing of bone defects by guided tissue regeneration. Plast.Reconstr.Surg.,81 :672~676,1988 35

a d b e c f (Fig.1) 3 HE a, b, c (d, e, f) a, b,c (d, e, f) 36

a c b d (Fig.2) HE a, b c, d (a, b) (c, d) 37

a c b d (Fig.3) 14 HE a, b c, d (a, b) (c,) 38

a d b e c f (Fig.4) PCNA a, b, c d, e, f (a, b, c) ( d, e, f) 39

a d b e c f (Fig.5) PCNA a, b, c d, e, f ( a, b, c) ( d, e, f) 40

a d b e c f (Fig.6) PCNA a, b, c d, e, f a, b, c (d, e, f) 41