B M B B B B B B

C C C C

CO 2Na N N H N N H N N Cl HN N Cl N N H N N N c C NH 2.HCl HO HO OH Me Me Me CO 2H N N NH 2 N Me Me

C C C C C C C

M M M M

Movie

BM BM BM

80M 80M

Golgi ER Adam17 Fringe Notch Signaling Signal sending cell S2 cleavage NUMB DVL G O-FUT Nucleus G JA G/Serrat e DLL G G G Notch Notch G Notch Notch SKIP HDAC SMRT CSL CTBP SHARP CIR Furin NEXT Deltex Hedgehog Signaling Ligand degradation or recycling PSEN1/2 APH1 S3 cleavage S1 cleavage Transcription Factors Phosphatases JA G/Serrat e G G DLL NECD γ-secretase complex N ICD NIC D HATs MAML CSL SKIP Hes 1/3/5 Hey1/2 MAG CNPase ER/Golgi NCSTN PEN2 BOC CDON PKA CK1 GSK3β P P SuFu P Gli 1/2/3 βtrcp Gli 1/2/3 FBXW11 Gli 3 Txn repressor Gli 3 HhN HhN HhN Patched SMO Off-State Cos2 Fu SuFu Gli 1/2/3 Gli 1/2 Txn activator Gli 1/2 CTBP DKK1 Wnt Signaling CER1 P NKD DVL FRAT Akt GSK3β P PP2A Axin APC β-catenin CK 1 P P P SOX17 LRP5/6 Wnt Wnt FZD WIF FRP ICAT Expression Expression Expression PreTα Myc p21 MASH-1 Gli 1/2 Hedgehog Dispatchted Ligands, Growth Factors, Etc. Cellular Proteins Wnt Gli1 CycD1 Myc Stem Cell Marker Antibody Packs #NBP1-42823 #NBP1-42824 #NBP1-42825 #NBP1-42826 Stem Cell Lines #NBP1-49595 #NBP1-41162 #NBA1-19349 #NBP1-96751 Pluripotent Stem Cell Transcription Factor Antibody Pack Essential ipsc Factor Antibody Pack Human ESC Marker Antibody Pack Neural Stem Cell Marker Antibody Pack CL-01 Human B Cell Line v6.5 Mouse Embryonic Stem Cell Line DGCR8 Knockout Mouse Embryonic Stem Cell Line Dicer Knockout Mouse Embryonic Stem Cell Line Stem Cell Growth Support #U0019 Stematrix #NBP1-78301 Laminin Protein for Neurite Growth Substratum On-State Patched Igf2 Cyclin E BMPs Self-Renewal PYGO BCL9 β-catenin TCF/LEF NLK P CTBP β-catenin Cell-Fate Determination BMP/Activin/Nodal Signaling FST Lefty CHRD β-catenin BMP Noggin Activin Nodal BMP Smad1/5/8 P Smad2/3 P Smad6/7 SMURF1 SMURF2 P P Smad1/5/8 Smad2/3 FBXW11 Smad4 Smad4 Ub Ub Ub Ub Ub Proteosomal degradation Myc Axin CyclinD1 DKK1 TGF-1 CD44 WISP1 MMP-7 BMPR1 BMPR2 P Smad1/5/8 Smad4 CBP Membrane Receptors Signal Transducers Expression Type I R Type II R P Smad2/3 Smad4 CBP Kinases Txn Regulators Expression Proteases Stem Cell Antibodies NB110-56924 beta Catenin NBP1-71663 CD31/PECAM1 NBP1-78292 Notch1 NBP1-19751 BMP2 NB600-1071 CD34 NB110-56923 Sonic Hedgehog 14-3-3 gamma Angiopoietin 1 beta Catenin BMP CD31/PECAM1 CD34 c-myc CXCR4 DKK EGF EPH Receptor B3 FGF GFAP IGF Jagged1 Musashi 1 N Cadherin Nestin Noggin Notch OCT4 PI3K PTEN SDF1 SFRP SMAD4 Sonic Hedgehog SOX2 SSEA1 SSEA3 STRO-1 TCF4 TGF alpha TGF beta TIE2 TRA-1-60 (R) TRA-1-81 VEGF WIF1 Wnt

E

Movie

CELLENDES Cell Environment Design

D D D D D D D D D D

A A

HO OH OH O OH O HN O OH OH

c c

1 New Feature 1 1 New Feature Amp ptac-1 2,734 bp lacz T T ORI T7 EcoRI SacI KpnI SmaI XhoI BamHI XbaI Sal I Pst I SphI HindIII Amp ptac-2 2,786 bp lacz T T ORI T7 NotI EcoRI SacI KpnI SmaI XhoI BamHI XbaI Sal I Pst I SphI HindIII NotI SP6 ptakn-2 2,739 bp Kan lacz T T ORI T7 NotI EcoRI SacI KpnI SmaI XhoI BamHI XbaI Sal I Pst I SphI HindIII NotI SP6

M

M

26 3 7 26 3 31 26 3 31 26 3 31 26 3 31 26 3 31 ZYMO RESEARCH total RNA Direct-zol 20% OFF 26 4 30 Kirkegaard & Perry Laboratories ELISA OZ Biosciences DreamFect Stem ZYMO RESEARCH DNA Worthington Biochemical Corporation STEMzyme p.10

A A A A A NOTE M D BC c A E