Gibco Gibco
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- こうき こうだ
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1 PSC PSC
2 Gibco Gibco
3 Contents Stem cell introduction Lipofectamine 3000 Transfection Reagent 7 Lipofectamine MessengerMAX Transfection Reagent 7 8 CRISPR 9 PSC PSC 10 Essential 8 10 Essential 8 Flex 11 PSC 11 PSC 12 PSC 12 TaqMan hpsc Scorecard Panel PSC Neural Induction Medium 15 PSC Dopaminergic Neuron Differentiation Kit 16 PSC Cardiomyocyte Differentiation Kit 17 PSC Definitive Endoderm Induction Kit Ordering information Ordering information 22-23
4 PSC ipsc PSC Gibco 1. ADSC MSC Gibco StemPro Human Adipose- Derived Stem Cells R Gibco StemPro BM Mesenchymal Stem Cells A15652 CD34+ PBMC Gibco StemPro Human Adipose- Derived Stem Cell Gibco StemPro MSC SFM XenoFree Gibco StemPro CD34+ Cell Kit A14059 Kit A R ADSC ADSC MSC CD34+ CytoTune PBMC T NSC N/A N/A Gibco StemPro Neural Stem Cells Gibco Human Dermal A15654 Fibroblasts, Adult C0135C Gibco StemPro-34 SFM PBMC Gibco CTS Gibco StemPro OpTmizer T Cell NSC SFM SFM A A T NSC Gibco Essential 8 Medium A
5 PSC ESC ipsc 2. Episomal ipsc Reprogramming Vectors* Epi5 Episomal ipsc Reprogramming Vectors** ipsc ipsc % % + Lin28 Neon Transfection System Neon Transfection System Neon Transfection System Lipofectamine 3000 Transfection Reqgent A14703 A15960 *Cellular Dynamics International **ipscira 7 RevitaCell Supplement PSC A B Alkaline Phosphatase Live Stain Invitrogen Alkaline Phosphatase Live Stain PSC AP Gibco RevitaCell Supplement HDFn C0045C CytoTune -ips 2.0 Sendai Reprogramming Kit MOI rhvtn-n RevitaCell Supplement (A) (B) 24 Essential 8 Medium PSC PSC Stem cell introduction PSC Ordering information 5
6 Invitrogen Lipofectamine 3. DNA mrna RNAi Protein Co-delivery Lipofectamine 3000 Transfection Reagent Lipofectamine RNAiMAX Transfection Reagent Lipofectamine MessengerMAX Transfection Reagent Neon Transfection System CRISPR Lipofectamine 3000 Transfection Reagent Lipofectamine MessengerMAX Transfection Reagent Lipofectamine CRISPRMAX Cas9 Transfection Reagent Not yet tested Not yet tested Lipofectamine 2000 DNA sirna DNA sirna co-transfection DNA Lipofectamine 3000 DNA sirna DNA sirna co-transfection sirna Lipofectamine RNAiMAX sirna microrna mrna Lipofectamine MessengerMAX mrna Cas9 Protein Lipofectamine CRISPRMAX Cas9 Protein Neon Transfection System DNA sirna microrna 0.1 ml 0.1 ml 0.1 ml 0.1 ml - L LMRNA001 CMAX
7 10 Lipofectamine 3000 Transfection Reagent Invitrogen Lipofectamine 3000 Transfection Reagent 3 1 DNA RNA ipsc Lipofectamine 3000 Neon Day 14 Day 17 BJ HDFn HDFa Lipofectamine MessengerMAX Transfection Reagent Invitrogen Lipofectamine MessengerMAX Transfection ReagentDNA mrna CRISPR10 A DNA:1.0 μg Lipofectamine 3000: SSEA4+/GFP+:42% GFP MFI: Lipofectamine 3000 H9 ESC (A) ipsc (B) SSEA4 SSEA4 Lipofectamine MessengerMAX mrna Reagent DNA mrna DNA:1.3 μg Lipofectamine 3000: SSEA4+/GFP+:69% GFP MFI: Lipofectamine 3000 Lipofectamine 3000 Neon Transfection SystemEpi5 BJ HDFn HDFa ipsc ipsc H9 ESC hnsc mesc 4. Lipofectamine MessengerMAX Gibco ips H9 ES ES DNA mrna Lipofectamine MessengerMAX mrna ng GFP mrna DNA 1250ng GFP DNA 24 GFP B Stem cell introduction PSC Ordering information 7
8 Clustered regularly interspaced short palindromic repeat CRISPR transcription activator-like TAL DNA CRISPR CRISPR TAL DNA 2 DSB 4 DNAHDR homology directed repair non-homologous end-joining NHEJ HDRDNA NHEJ DSB 4 GeneArt CRISPR all-in-one plasmid Cas9 grna GeneArt CRISPR mrna GeneArt Cas9 Protein GeneArt CRISPR lentiviral libraries CRISPR grna GeneArt TAL effectors TAL IP TALEN grna PAM NGG PAM NGG PAM NGG PAM NGG DNA RNA-DNA RNA-DNA RNA-DNA RNA-DNA -DNA 8
9 CRISPR CRISPR-Cas9 CRISPR-Cas9 CRISPR-Cas9 RNA RNA Cas9 5 CRISPR CRISPR-Cas9 all-in-one expression plasmid Cas9 mrna Cas9 proteincrispr lentiviral library service CRISPR-Cas9 GeneArt CRISPR vector with OFP reporter GeneArt CRISPR vector with CD4 reporter GeneArt CRISPR all-in-one plasmid Lipofectamine 3000 Transfection Reagent Control ipscs grna + Cas protein IVT grna Cas9 mrna GeneArt CRISPR mrna Lipofectamine MessengerMAX Transfection Reagent Control H9 ESCs grna + Cas protein Cleavage % Invitrogen GeneArt Cas9/gRNA RNP Gibco Human ips H9 ESGeneArt Cas9/ grna RNP Invitrogen GeneArt Genomic Cleavage Detection GCD GeneArt Genomic Cleavage Detection Kit GeneArt Genomic Cleavage Selection Kit GeneArt protein Lipofectamine CRISPRMAX Cas9 Transfection Reagent IVT grna Cas9 protein GeneArt Precision grna Synthesis Kit RNA Invitrogen Lipofectamine CRISPRMAX 55500ng Invitrogen GeneArt Platinum Cas9 Nuclease ips 24 Gibco Opti-MEM ( ) GCD Stem cell introduction PSC Ordering information 9
10 PSC PSC Gibco KnockOut Serum Replacement KSR Gibco Essential 8 Gibco PSC 5. PSC KnockOut Serum Replacement Gibco StemPro hesc SFM A Mouse ICR Inacivated Embryonic Fibroblasts A24903 PSC Essential 8 A Essential 8 Flex A Gibco Geltrex hesc-qualified Gibco Vitronectin VTN-N Ready-To-Use Reduced Growth Recombinant Human Protein, Factor Basement Membran Matrix Truncated A A14700 Gibco Collagenase IV Gibco StemPro EZPassage Disposable Stem Cell Passaging Tool Collagenase IV StemPro EZPassage Disposable Stem Cell Passaging Tool Gibco Versene Solution CTS KnockOut SR XenoFree Medium Gibco CTS CELLstart Substrate A Gibco CTS TrypLE Select Enzyme A EZPassage Disposable Stem Cell Passaging Tool Essential 8 Essential 8 James Thomsons Essential 8 PSC 8 ipsc BSA Essential 8 PSC PSC Essential 8 PSC Essential 8 ipsc Essential 8 + RevitaCell Supplement Essential 8 Flex PSC Essential 8 Adaptation Kit PSC 10
11 PSC Essential 8 Flex Gibco Essential 8 Flex FGF2 PSC 2 E8 Essential 8 Essential 8 7 Day 1 Day 2 Day 3 Day 4 Essential 8 Flex Day 1 Day 2 Day 4 Day 5 Day 5 PSC Day 6 Day 7 ECM Gibco 6. ESC ipsc Gibco Geltrex LDEV-Free hescqualifi ed Reduced Growth Factor Basement Membrane Matrix A Geltrex hesc-qualifi ed Readyto-Use Reduced Growth Factor Basement Membrane Matrix A FGF Essential 8 Flex h 7. PSC FGF2 PSC Essential 8 Flex FGF2 Essential 8 Vitronectin (VTN-N) rhlaminin-521 Recombinant Human Protein, A29249 Truncated A14700 StemPro hesc SFM Essential 8 EHS StemPro hesc SFM Essential 8 EHS Essential 8 Essential 8 Stem cell introduction PSC Ordering information 11
12 PSC Gibco RevitaCell SupplementPSC 7. Gibco PSC Cryopreservation Kit PSC Gibco Synth-a-Freeze Cryopreservation Medium ipsc ESC PBMC ipsc MSC NSC Gibco Recovery Cell Culture Freezing Medium CHO-S CHO-K1 HEK 293 Jurkat NIH 3T3 FBS Ready-to-use RevitaCell Supplement CTS N/A Gibco CTS Synth-a-Freeze N/A Cryopreservation Medium A A A PSC ipsc Alkaline Phosphatase Live Stain Applied Biosystems TaqMan hpsc Scorecard Panel 8. Alkaline Phosphatase Live Stain A14353 PSC ordering information 22 ESC ipsc Applied Biosystems TaqMan human stem cell pluripotency array PSC TaqMan hpsc Scorecard Panel 13 ordering information 22 PSC PSC 20 PSC EVOS FLoid Cell Imager N/A N/A 12
![TaqMan hpsc Scorecard Panel TaqMan hpsc Scorecard PanelqPCR hpsc ScorecardAlexander Meissner [1] [2] 93 www.thermofisher.](/docs-images/88/116157713/images/13-0.jpg "com/scorecard Algorithm sco re 5 4 3 2 1 0-1 -2-3 -4 Self-renewal Ectoderm Mesoderm Endoderm H9-ESC-...50-Day0 H9-EB-Day2 H9-EB-Day4 H9-EB-Day7 8.")
13 TaqMan hpsc Scorecard Panel TaqMan hpsc Scorecard PanelqPCR hpsc ScorecardAlexander Meissner [1] [2] 93 Algorithm sco re Self-renewal Ectoderm Mesoderm Endoderm H9-ESC Day0 H9-EB-Day2 H9-EB-Day4 H9-EB-Day7 8. hpsc Scorecard Analysis Software Invitrogen Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit 4 OCT4 SOX2 SSEA4 TRA-1-60 DNA Invitrogen 3-Germ Layer Immunocytochemistry Kit 3 III - EB1_Proto3_ eds 10. H9-EB-Day7 H9-ESC Day0 Selfrenewal Ectoderm Mesoderm Endoderm Selfrenewal Ectoderm Mesoderm Endoderm 9. ICC Stem cell introduction PSC Ordering information 13
14 Gibco 9 Gibco PSC Neural Induction Medium A Geltrex LDEV-Free hescqualified Reduced Growth Factor Basement Membrane Matrix A Gibco PSC Dopaminergic Neuron Dif ferentiation Kit A Vitronectin (VTN-N) Recombinant Human Protein, Truncated A14700 Laminin Mouse Protein, Natural Gibco PSC Cardiomyocyte Differentiation Kit A Geltrex LDEV-Free hescqualified Reduced Growth Factor Basement Membrane Matrix A Gibco PSC Definitive Endoderm Induction Kit A27654SA Vitronectin (VTN-N) Recombinant Human Protein, Truncated A X supplement/500 ml basal, serum-free Serum-free Ready-to-use, xeno-free Ready-to-use, xeno-free 14
15 7 PSC PSC Neural Induction Medium Gibco PSC Neural Induction Medium 7 EB NSC 12PSC Neural Induction Medium NSCNSC 13 Gibco PSC Neural Induction Medium Oct4 Nestin Sox1 Sox % Human NSC Immunocytochemistry Kit hpsc NSC Invitrogen Human Neural Stem Cell Immunocytochemistry Kit4 nestin PAX6 SOX1 SOX2 DNA Day hpsc 12. Gibco PSC Neural Induction Medium EB 13.Gibco PSC Neural Induction Medium NSC NSC NSC Day NSC Stem cell introduction PSC Ordering information 15
16 ips PSC Dopaminergic Neuron Differentiation Kit Gibco PSC Dopaminergic Neuron Differentiation Kit (PSCs) PSC Dopaminergic Neuron Differentiation Kit A TH B FOXA2 C TH and FOXA2 14. Dopaminergic Neuron Maturation Medium( )14 Invitrogen Human Dopaminergic Neuron Immunocytochemistry Kit( A29515) THFOXA2 A. TH ( ) B. FOXA2 ( ) Invitrogen NucBlue (DAPI DNA ) ( ) C. TH FOXA2 ( ) 0 10 / Essential % specification 2 2 Day16 / Day20 2 Day Essential Human Dopaminergic Neuron Immunocytochemistry Kit Invitrogen Human Dopaminergic Neuron Immunocytochemistry Kit 3 OTX2 FoxA2 (TH) DNA 16
17 8 PSC Cardiomyocyte Differentiation Kit Gibco PSC Cardiomyocyte Differentiation Kit 8 3 PSC 3 1X % TNNT2+ cells Beat period (s) 100% 80% 60% 40% 20% % H9 hesc ipsc (CytoTune/Sendai) PSC lines Time (s) Gibco Human Episomal ipsc Human Cardiomyocyte Immunocytochemistry Kit Invitrogen Human Cardiomyocyte Immunocytochemistry Kit 2 NKX2.5 TNNT2/ ctnt DNA Fibroblast-derived control HCM (R663H MYH7) CD71 + -derived control HCM (R145W TNNI3) 16. Gibco TrypLE Gibco Geltrex Essential 8 3 PSC Cardiomyocyte Differentiation Kit2 TNNT2+ 17.PSC Cardiomyocyte Differentation Kit ips Maestro Multielectrode Array (MEA) (Axion Biosystems) 1 Stem cell introduction PSC Ordering information 17
18 2 PSC Defi nitive Endoderm Induction Kit Gibco PSC Defi nitive Endoderm Induction Kit CXCR4+/PDGFR 19 1X PSC 90 SOX17 FOXA220 H9-ESC Human Episomal ipsc Line CytoTune-iPS 2.0 Sendai-generated line CytoTune-iPS 1.0 Sendai-generated line 18.PSC Definitive Endoderm Induction Kit CytoTune hesc ipsc 90 CXCR4+/PDGFR PSC Definitive Endoderm Induction Kit hpsc A B Day S hpsc hpsc 1 2 Day PSC Definitive Endoderm Induction Kit SOX17 FOXA2 Oct4 20.PSC Definitive Endoderm Induction Kit hesc 3 SOX17 FOXA2 Oct4DAPI 18
19 Gibco Gibco ( 95%) <0.1 ng GibcoGibco Fibroblast Growth FactorBasic bfgf, FGF-basic, FGF 2 FGF-basicES Epidermal Growth Factor EGF EGFin vivo Granulocyte Macrophage Colony-Stimulating Factor GM-CSF GM-CSF Activin A Activin A Tumor Necrosis Factor TNF Gibco TNF TNF Vascular Endothelial Cell Growth Factor VEGF VEGF Stem cell introduction PSC Ordering information 19
20 Invitrogen Qdot Invitrogen Alexa Fluor nvitrogen Alexa Fluor Invitrogen TaqMan PreAmp Cells-to-C T Kit Applied Biosystems Protein Expression Invitrogen RNA Invitrogen DNA Alkaline Phosphatase Live Stain Invitrogen cell health assays Applied Biosystems TaqMan Invitrogen Novex Invitrogen Novex Invitrogen Novex ELISA Invitrogen Bolt qrt-pcr Applied Biosystems TaqMan Applied Biosystems TaqMan mirna Assays Applied Biosystems TaqMan SNP Assays Applied Biosystems TaqMan CNV Assays Applied Biosystems AuthentiFiler PCR Amplification Kit Ion Ampliseq ChIP ICC IF IHC ELISA Invitrogen 40,
21 EVOS Invitrogen EVOS sher.com/evos Countess II Invitrogen Countess II 10 Countess FL sher.com/countess StepOnePlus PCR Applied Biosystems StepOnePlus PCR TaqMan hpsc Scorecard Panel sher.com/steponeplus QuantStudio ( PCR/ PCR) Applied Biosystems QuantStudio PCR PCR sher.com/quantstudio Neon Transfection System Invitrogen Neon Transfection System sher.com/neon Attune NxT Invitrogen Attune NxT Invitrogen Attune Autosampler Attune NxT sher.com/attune Ion S5 / Ion S5 XL 15 NGS 3 200bp bp 4 sher.com/ions5 Ion PGM Ion AmpliSeq FFPE sher.com/pgm Stem cell introduction PSC Ordering information 21
22 Ordering information StemPro Human Adipose-Derived Stem Cells R ,000 StemPro Human Adipose-Derived Stem Cell Kit R ,800 StemPro BM Mesenchymal Stem Cells A x 10 6 cells 87,600 StemPro MSC SFM XenoFree A ,300 StemPro CD34+ Cell Kit A x 10 6 cells/ml 151,200 StemPro-34 SFM ml 31,300 Gibco CTS OpTmizer T Cell Expansion SFM A ,000 ml 16,400 CTS Immune Cell Serum Replacement A ml 22,000 StemPro Neural Stem Cells A x 10 6 cells 78,400 StemPro NSC SFM A ,000 Human Dermal Fibroblasts, Adult C0135C 1 53,700 Epi5 Episomal ipsc Reprogramming Kit A ,000 Episomal ipsc Reprogramming Vectors A tube 90,400 Lipofectamine 3000 Transfection Reagent L ml 9,900 L ml 70,800 Lipofectamine MessengerMAX Transfection Reagent LMRNA ml 10,400 LMRNA ml 72,600 Neon Transfection System Starter Pack MPK5000S 1 1,164,000 Neon Transfection System MPK ,000,000 Lipofectamine CRISPRMAX Cas9 Transfection Reagent CMAX ml 10,800 CMAX ml 71,800 Lipofectamine RNAiMAX Transfection Reagent ml 72,700 Invivofectamine 3.0 Reagent IVF ml 158,400 GeneArt CRISPR Nuclease mrna A μg 84,000 GeneArt CRISPR Nuclease Vector with CD4 Enrichment Kit A ,200 GeneArt CRISPR Nuclease Vector with OFP Reporter Kit A ,200 GeneArt CRISPR T7 Strings DNA GeneArt CRISPR U6 Strings DNA GeneArt Genomic Cleavage Detection Kit A ,100 GeneArt Genomic Cleavage Selection Kit A ,100 GeneArt Platinum Cas9 Nuclease B μg 43,000 B μg 85,000 B μg 180,000 GeneArt Precision grna Synthesis Kit A ,000 GeneArt TALs PSC Essential 8 Flex Medium A ml 31,000 Essential 8 Medium A ml 24,500 RevitaCell Supplement A ml 14,300 Essential 8 Adaptation Kit A ,900 Collagenase IV g 38,700 StemPro EZPassage Disposable Stem Cell Passaging Tool ,200 StemPro hesc SFM A ,200 Gibco Versene Solution ml 3,200 Essential 6 Medium A ml 19,200 KnockOut Serum Replacement ml 14, ml 52,800 CTS KnockOut SR XenoFree Medium ml 18,600 CTS TrypLE Select Enzyme A ml 4,500 Vitronectin (VTN-N) Recombinant Human Protein, Truncated A ml 6,000 rhlaminin-521 A μg 15,800 Gibco Mouse (ICR) Inactivated Embryonic Fibroblasts A ml 3,300 Geltrex hesc-qualified Ready-To-Use Reduced Growth Factor Basement Membrane Matrix A ml 5,900 Geltrex LDEV-Free hesc-qualified Reduced Growth Factor Basement Membrane Matrix A ml 5,200 CTS CELLstart Substrate A ml 38,200 PSC Cryopreservation Kit A ml 21,900 CTS Synth-a-Freeze Cryopreservation Medium A ml 11,700 Recovery Cell Culture Freezing Medium ml 12,600 RevitaCell Supplement A ml 14,300 Synth-a-Freeze Cryopreservation Medium A ml 13,100 22
23 PSC Neural Induction Medium A ml 58,800 PSC Dopaminergic Neuron Differentiation Kit A ,000 PSC Cardiomyocyte Differentiation Kit A ,800 PSC Definitive Endoderm Induction Kit A27654-SA 1 85,300 bfgf Recombinant Human Protein μg 51,300 EGF Recombinant Human Protein PHG μg 14,200 GM-CSF Recombinant Human Protein PHC μg 37,700 Activin A Recombinant Human Protein PHC μg 66,800 TNF Recombinant Human Protein PHC μg 21,200 VEGF Recombinant Human Protein PHC μg 27,000 TaqMan hpsc Scorecard Kit, Fast 96-well A x 96 well 96,000 TaqMan hpsc Scorecard Panel, Fast 96-well A x 96 well 70,500 TaqMan hpsc Scorecard Kit, 384-well A x 384 well 89,000 TaqMan hpsc Scorecard Panel, 384-well A x 384 well 50,600 Alkaline Phosphatase Live Stain A μl 32,800 TaqMan Human Stem Cell Pluripotency Array cards 227,000 AlexaFluor 555 Tra-1-60 Live Cell Imaging Kit A ,700 AlexaFluor 594 Tra-1-60 Live Cell Imaging Kit A ,700 AlexaFluor 488 CD44 Live Cell Imaging Kit A ,700 AlexaFluor 488 Tra-1-60 Live Cell Imaging Kit A ,700 PSC Immunocytochemistry Kit (OCT4, SSEA4) A ,100 PSC Immunocytochemistry Kit (SOX2, TRA-1-60) A ,100 Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit A ,400 3-Germ Layer Immunocytochemistry Kit A ,400 Human Neural Stem Cell Immunocytochemistry Kit A ,800 Human Dopaminergic Neuron Immunocytochemistry Kit A ,400 Human Cardiomyocyte Immunocytochemistry Kit A ,000 c-myc Antibody MA μg 56,500 DNMT3b Antibody μg 61,000 KLF4 Antibody μg 61,000 LIN28 Antibody MA μg 56,500 NANOG Antibody MA μg 61,000 OCT4 Antibody A μl 51,000 SALL4 Antibody μg 61,000 SOX 2 Antibody μg 71,400 SSEA-1 Antibody MA μg 56,500 SSEA-3 Antibody MA μg 56,500 SSEA-4 Antibody MA μg 56,500 SSEA-5 Antibody MA μg 61,000 TRA-1-60 Antibody μg 61,000 TRA-1-81 Antibody μg 61,000 Gibco Gibco cgmp* CTS cgmp* cgmp* 10L10,000L DPM 8,000kg Advanced Granulation Technology AGT 50kg6,000kg Gibco Stem cell introduction PSC Ordering information *cgmp CFR Title 21, part210 current Good Manufacturing PracticeGMP 23
24 1. Bock C, Kiskinis E, Verstappen G et al. (2011) Reference Maps of human ES and ips cell variation enable high-throughput characterization of pluripotent cell lines. Cell 144(3): Tsankov AM, Akopian V, Pop R et al. (2015) A qpcr Scorecard quantifies the differentiation potential of human pluripotent stem cells. Nat Biotechnol 33(11): For Research Use only. Not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. CytoTune is a trademark of DNAVEC Corporation. Essential 6 and Essential 8 are trademarks of Cellular Dynamics International, Inc. TaqMan is a trademark of Roche Molecular Systems, Inc., used under permission and license. Axion Biosystems and Maestro are trademarks of Axion Biosystems GIB070-A1608TY jptech@thermofisher.com TEL FAX TEL FAX facebook.com/thermofisherjapan
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