A13 C A139 A137 A131 A138 V CV FC/SMA 2.51/2.5 MM FF 4 4 5 5 6 A11971 A136 ND A132 1 mm ND1 % 1 %2 6 7 7 A133-9 15 mm 8 A134-9 A135 A135-9 A1127 A1858 A176 A1859 C A11213 C A9865 45V R T 11 15 mm XY XZ FC 1 mm 25mmC C C CC 9 1 1 11 12, 13 14 15 16 16 2

C A11214 A1126 45C 17 18 2 19 19 19 2 2 21 22 22 23 24 V A13-1 H172/H1721 H119/H1191 H1722/H1723 H1192/H1193 H11526 H1176/H1256 C C A9865 H1682 H1189 H12386 H1799 V A13-2 H7826/H7827/H7828 H12775 V C A139 H7421/H7422 C C* H11461/H11462/H8259 * C 3

A13-1 A13-2 8 mm 12 mm 18 g H172/H1721/H1722/H1723/H119 H1191/H1192/H1193/H11526/H1176 A13-1 H1256/H1682/H1189/H12386 H1799 A13-2 H7826/H7827/H7828/H12775 A13-1 A13-2 38.2 ± 1. 38.2 ± 1. 23. ±.1 22. ±.1 18. ±.1 26. ±.1 15. ±.1 1.8 ±.1 15. ±.1 8 4 27. ±.1 18. ±.1 15.5 ±.1 15. ±.1 1.8 ±.1 4 M2 L12.5 2 M3 3 M4 5 TPMOA5JA 4 M2 L12 2 M3 4 M4 5.5 8 4 TPMOA85JA A139 C 8 mm 27 g 4 C 38.2 ± 1. 22. ±.1 32 22. ±.1 16.5 ±.2 3.5 ±.1 1.4 C 1 mm 2. CC 3. 4 4 M2 L16 TACCA287JA 4

A137 TACCB94JB 1 8 A137-1 22. ±.1 7. ±.1 (8) 4.6 ±.1 (%) 6 4 22. ±.1 ( 16) 2 A137 / A137-1 A137-1 / A137-11 2 3 4 5 6 7 8 (nm) L A137/-1 : L=9. A137-1/-11: L=13.5 1 µm NA=.2 1 mm 4.9 mm 15 mm 5.4 mm SMA FC TACCA27JB TACCB95JB 1 8 A131 8 mm C 4 nm7 nm 2 4 nm7 nm 36. ±.2 3. ±.2 9. ±.1 43 g ( 16) (%) 6 4 C 2 44.4 ±.3 (8) 44.4 ±.3 8 (8) TACCA271JA 2 ( 16) 3 4 5 6 7 8 (nm) TACCC127JA 5

A138-1 A138-2 8 mm 8 mm 1 g 25 g A138-1 A138-2 A138-1 9. ±.1 5. ±.1 A138-2 22. ±.1 16 22. ±.1 38.2 ± 1. 22. ±.1 (8) (8) TACCA284JA 22. ±.1 19. ±.2 TACCA285JA + 12.5 mm A11971 8 mm 1 mm 56 mm (H) 2 S2.5 26 g A13-1 H1799-xxx 2 4.5 38.2 ± 1. 21 22 22 85 43. ±.2 21 25. ±.1 14 ± 1 2 A (25) 28.1 19.5 (1) A' 2 56 8.1 25. ±.1 8.1 32 18. ±.1 8.1 16 18. ±.1 15. ±.1 5. ±.1 6 2 M3 L16 3 A A' TPMOA86JA A133-9 A137-xx A11971 6

A136 8 mm 48 g 24. ±.1 38.2 ± 1. 22. ±.1 22. ±.1 21. ±.1 ( 16) 16. + -.15 49. ±.2 12. ±.1 2 16. ±.1 3. ±.1 (8) 9. ±.1 TACCA288JA A132-1 A132-11 ND 1 % 1 % 4 nm7 nm 8 mm 25 g 2. ±.1 (8) 2 9. ±.1 8 TACCB87JA 6 22. ±.1 (%) 4 2 A132-1 A132-11 38.2 ± 1. 22. ±.1 ( 16) TACCA272JA 3 4 5 6 7 (nm) 7

A133-9 8 mm 12.7 mm1/215 mm15 mm 24 g 1.5 mm5 mm 2. ±.1 (8) 2 9. ±.1 22. ±.1 38.2 ± 1. 22. ±.1 ( 16) TACCA272JA A133-9 A133-9 ND A132-xx PMT A13-1 A137-xx A137-xx A13-1 TPMOC211JB 8

A134-9 8 mm : 25 mm26 mm (W) 35 mm38 mm (H) :.9 mm1.1 mm1 mm S2. 139 g 38.2 ± 1. 36 ( 16) 26. ±.5 9. ±.1 2 3.2 26. ±.5 27. ±.5 45 (8) ( 16) 9. ±.1 35. ±.5 (8) 62. ±.1 TPMOA98JA A133-9 A13-1 A13-1 A137-xx A134-9 9

A135 8 mm 4 nm7 nm 63 g 11. ±.1 2 26. ±.5 22. ±.1 TACCB89JC 1 ( 16) 9. ±.1 (%) 8 6 4 P 3. ±.2 (8) 38.2 ± 1. 9. ±.1 ( 16) 2 S 13. ±.1 22. ±.1 37. ±.2 (8) TACCA273JA 4 45 5 55 6 65 7 (nm) A135-9 8 mm 2 S.89, S1.5 15 mm 15 mm 15 mm 55 g 2 38.2 ± 1. 22. 9. ±.1 26. ( 16) 8 13. 17 ±.1 15 15 15 9. ±.1 22. ±.1 17 ±.1 15. ±.4 11. ±.1 2. ±.1 4 M2 L8 (39) (8) (26) ( 16) 22. 2. ±.1 4 M3 6 TPMOA68JA 1

A1127 XY : 16 mm ±1.2.25 mm : 1.58 mm 1.6 mm S2.5 18 g 15 mm TPMOB233JA 1 C 2 M3 3 15. ±.1 M4 5 5. 11.5 ± 1.5 Y : ±1.2 5. 11.5 ± 1.5 12. (27.526 ±.3) (8.) 17.746 (.22) 17.526 1. X : 1.2 12. (25) 5. (18) 43. ±.2 45. 29. C 4.6 ( 16) 8 3. 22.95 5. 2 4.5 6. 2 M3 L16 : 16 : 1.6 TPMOA7JA (%) 6 4 2 3 4 5 6 7 8 (nm) A133-9 A13-1 A1127 C 11

X Z C A1858 ±6.5 mm ±3 mm 2 * P.14 M2.32 P.76 * 45 mm 4 kg 11 5 81 135 2 C (38) 16 245 14 TPMOA49JA 4 6 24 25 24 25 1 9 mm 9 mm 1 mm 2 6 mm 6 mm 18 mm 3 6 mm 6 mm 25 mm 2 1 1 127 8 8 (38) 2538 (173) 16173 TPMOA66JA 12

13 *

A176 A176 XYZ ±1 mm 3 mm FC M2.32 P.76 3 mm35 mm 45 mm S3. 42 g C TPMOC29JA A176 69 43 TPMOC21JA 45 7 32 52 2 M3 4 TPMOA51JA 1. 2 2. 2 Y TPMOC223JA X Z 3. M2.32 P.76 4. 21 XYZ 1 14

A1859 (%) TPMOB251JA 1 8 6 4 2 3 4 5 6 7 8 9 1 (nm) C M3 C A1859-1 A1859 A1859-1 1 mm 15 mm 24 mm C S1.5 4 nm7 nm 1/3 1/2 C M3 C 13 g 25 g 32 3 42 4 4 4 93 (92 94) 17.526 TPMOA52JA A1859 / A1859-1 TPMOC224JA 4 4 96 (95 97) 17.526 TPMOA53JA 15

A11213 A11213-1 C 2 A11213 A11213-1 2 mm S1.5 24 mm26 mm 25 mm25.4 mm 5 mmmax. 2 mm5 mm 29 g 3 g C A9865 C A11213-xx 35 C 4 2 4 3 M3 L4 2 C TPMOA54JA C A139 35 C A9865 C A11213-1 5 2 4 3 M3 L4 TPMOA55JA A137-xx A9865 8 mm 7 g 4 3.2 4 2.1 C 16 H172/H1721/H1722/H1723/H119/H1191/H1192 H1193/H11526/H1176/H1256/H1682/H1189/H12386 H1799 14 14 3 5. 9 7..5 TPMOA56JA

A11214 2 mm 25 mm26 mm (W) 35 mm38 mm (H).9 mm1.1 mm 1 mm S2. 84 g C5 C5 2 3.2 26. ±.5 C5 26. ±.5 18 2 4 36 TPMOA57JB C A11213 C A9865 C A9865 A137-xx C A11214 C A139 C A11213 C A9865 C A11214 C A139 A137-xx 17

: 25 mm 25.4 mm A1126 8 mm 9 1 5 2 55 g : 1 mm 8 mm 19 29 S1.5 34. 22. 38.2 ± 1. 34. 22. (9 ) (23 ± 1) 5 37. ±.3 (8) : 25.4 : 1 8 9. ±.1 ( 16) 22. 32 2. ±.1 2 17 22. 4 M2 L8 24 2 M3 3 TACCA69JA 18

A137 A13-1 PMT A133-9 LED TPMOC213JB A131 A13-1 PMT L1 L2 LED NA=.5.98 mm 5 mm1 L1, L2 L1 L2 5 mm 8 % 1 % 9 mm 59 % 59 % 25 mm 28 % 13 % 35 mm 2 % 7 % TPMOC214JA C C A11213-1 C A9865 17.526 C A9865 17.526 PMT PMT L* 7 1 L* 7 25 * L: PMT TPMOC216JA 19

A176 A1858 C A11213 A1859-1 A1127 A13-1 PMT C A11213 C (A1859-1 C A11214 A133-9 TPMOC217JB A1859 EM-CCD * RMSC 2 TPMOC215JA

A114-35-11 L9588-3 C A11213-1 EM-CCD A1859-1 C A11213 C A11214 A1858 C A11213 * C SX-6 25 mm TPMOC218JB C A1859-1 CCD A1859-1 LED A1858 TPMOC219JA 21

C A131 A131 PMT A1858 A133-9 A13-1 TPMOC255JB A176 C A11213 A1858 C A131 A138-2 A137-1 C A11214 A131 * * TPMOC221JA 22

A1858 A114-35-11 L9588-3 C A11213-1 A1859-1 EM-CCD C A11213 C A11213 C A11213 C A11214 C A9865 PMT C A11213-1 C A11213 A176 * C SX-6 C A18581 TPMOC222JB 23

633 nm 532 nm A134-9 DM 66 TACCB9JA 1 9 8 7 A133-9 575/3 nm A133-9 7/6 nm (%) 6 5 4 Cy3 Alexa 546 Cy5 Alexa 647 TACCC128JB 3 2 1 4 5 6 7 8 (nm) TACCB91JA 1 9 8 45 nm A134-9 DM 55 A133-9 46/6 nm A133-9 53/4 nm (%) 7 6 5 4 3 2 1 DAPI Hoechst CASY FITC 4 5 6 7 8 (nm) TACCC129JB TACCB92JA 1 9 488 nm A133-9 5 nmlp A134-9 DM 565 A133-9 53/4 nm A134-9 DM 6 A133-9 575/3 nm A134-9 DM 66 A133-9 63/6 nm A133-9 7/6 nm (%) 8 7 6 5 4 3 2 FITC GFP PE CASY Texas Red PI Cy5 7AAD 1 4 5 6 7 8 TACCC13JB (nm) 24

FWHM 428 65 465 26 522 35 66 3 nm nm TACCB114JA 1 1 TACCB115JA 1.2 TACCB12JA.8.8 1.6.4.6.4.8.6.4.2.2.2 35 4 45 5 55 6 65 7 75 35 4 45 5 55 6 65 7 75 6 12 18 24 3 (nm) (nm) (h) TACCB116JA 1 TACCB119JA 1 12 TACCB121JA 11.5.8.8 11.6.4.6.4 (%) 1.5 1 99.5.2.2 99 98.5 35 4 45 5 55 6 65 7 75 35 4 45 5 55 6 65 7 75 98 5 1 15 2 25 3 35 4 45 5 (nm) (nm) ( C) 24 L11494-xxx 8.5 2 M2 12 A138-1 14 12 ON OFF ON 14 7 65 A13-1 SR41 TACCA311JA 25

* : P.6 (V/nW) TPMOB213JA 1.1.1 H1799-11 H1799-12 H1799-1 H1799-2 / * * * 14. ±.2 8-1 -11-2 -12 19 nm / 32 nm 11 nm +5 V +5.5 V +4.5 V.1 V/nA / DC 1 Hz 1 V/nA / DC 1 Hz.1 V/nA / DC 1 khz 8 mm +5 C +5 C -2 C +5 C S1337-11BQ S1337-11BR S1337-11BQ S1337-11BR H1799-2/-12 /.1 V/nA / DC1 Hz.1 2 3 4 5 6 7 8 9 1 11 25. ±.5 11 4 M2 (nm) 18. ±.5 GND : AWG26 (+5 V) : AWG26 : RG-174/U 5. ±.5 45 ± 2 TPMOA48JA 26

A 488 nm 632 nm 5 mm 21.5 mm 4 mm 2 mm 8 Lp/mm 4 Hz B 7 % Al Af488 nm / 532 nm / 632 nm3 B A F E DAQ X Y B C D G H PC X USB, PCI 1 Y 1 PMT DAQ (DAQ) 1/4 A B C D E F G H C C1516 A139 A138-1 A1126 A137 A138-2 A135-9 A13-1 1 1 1 1 1 1 1 1 27

TPMO158J7 FEB. 219 IP (2)