! One Point 1 20% 1 1 2 2 3 20% 4 5 20ml ÄKTAexplorer 10S, ÄKTAexplorer 10XT, ÄKTApurifier 10, ÄKTApurifier UPC 10 2µm 1 ml/min 0.35 MPa 2M NaOH 18-1120-94 2 ÄKTAexplorer, ÄKTApurifier ÄKTAexplorer 100, ÄKTApurifier 100, ÄKTApurifier UPC 100 18-1027-11 ÄKTA Sample Fiber UV Sample Fiber UV 10% Decon 90, Deconex 11, RBS 25 20 1M NaOH30% 2-23
! UV UV-900 Check Lamp Intensity 210mm 85% 300nm 95% Check Lamp Run Time 200h ÄKTAexplorer, ÄKTApurifier Check Check Lamp Intensity 20% CheckCheck Lamp Run Time 4,000 2,000 4,000 2,000 Q A 18-1132-75 Check valve Q P-950 A Q A Q 280 nm254 nm A TEL 03-5331-9336 Q ph A ph electrode, round tip, UPC-90018-1111-26 Q UV UV A1 mau UV UV A2 UV-900 UV Q HiLoad A ÄKTA PEEK Union M6 female/1/16female 18-1123-94 SRTC-2 19-2143-01 SRTC-2 M6 female/m6 female Union 1/16female / M6 male 18-1112-57 Q HiPrep A ÄKTAexplorer 10S ÄKTApurifier HiPrep Back pressure 0.75 mmdelay Volume Q PC No System DiskWindows A USB 24
Q A P-900 2 Q 5 Waste A 5 Q 1 ml/min 0.35 MPa A p.23 Q Frac-900Frac-920 A 19-6067-02 Q Superloop A1 O-ring O-ring A2 Superloop ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.21 p.36 p.36 ÄKTAexplorer, ÄKTApurifier 25
ÄKTAFPLC ÄKTAFPLC 1 2 1 PumpSeal 1 1 Injection Valve Accumulator Seal Frac-950 1 FracTube Sensor Frac-900/920 1 UV Filter 1 UV Filter 1 Fraction UV P-920 Pump Seal FLOW FLOW Conductivity Cell Conductivity Cell PC Sample Pump Sample Pump Pump Switch valve A,B Fraction Collector ph 2 ph Aceton Frac Parameters Delay vol. UNICORN Fraction 1 Pump Seal 4 Pump Valve 2 Inlet Filter 4 Distributing Block INV-907 1 Channel Plate INV-907 1 On Line Filter Kit 1 26
! One Point 1 2 Concentration 50%B 20ml/min 3 4 5 6 7 8 1-7 12 ÄKTAFPLC 18-1027-11 UV UV 10% Decon 90, Deconex 11, RBS 25 20 1M NaOH30% 2-! Check Lamp Intensity R 215.5 S 214.7mV Check Lamp Run Time Hg 2300h Zn 340H CheckCheck Lamp Intensity Hg RReference 150 mv280 nm CheckCheck Lamp Run Time Hg 280 nm 3,500 Zn Lamp Run Time 2,000 27
Q A Q ph A ph electrode, round tip, UPC-90018-1111-26 ÄKTAFPLC Q UV UV A mau UV UV Q HiLoad A ÄKTA PEEK Union M6 female/1/16female 18-1123-94 SRTC-2 19-2143-01 SRTC-2 M6 female/m6 female Union 1/16female / M6 male 18-1112-57 Q PC No System DiskWindows A USB Q 5 Waste A 5 Q 1 ml/min 0.35 MPa A p.27 Q Frac-900Frac-920 A 19-6067-02 Q Superloop A1 O-ring O-ring A2 Superloop ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.21 p.36 p.36 28
ÄKTAprime, ÄKTAprime plus 1 Piston cpl 3 Ballstopper & Seal 1 O-rings kit,p-960 1 Glass Tube Gasket 3 CPU Injection Valve Gradient Valve Buffer Valve Mixer Fraction Collector Cond Monitor UV Monitor Pump FLOW FLOW Conductivity Cell Conductivity Cell ph UV Delay Vol. Fraction Drop Counter ÄKTAprime, ÄKTAprime plus! Check Lamp Intensity R 215.5 S 214.7mV Check Lamp Run Time Hg 2300h Zn 340H CheckCheck Lamp Intensity Hg RReference 150 mv280 nm CheckCheck Lamp Run Time Hg 280 nm 3,500 29
ÄKTAprime, ÄKTAprime plus Q UV A UV 1 UV 1 M NaOH 1 ml / min 30 UV 96% System Wash method method Q A ÄKTAprime A 1 28 18-1112-52 System Wash method 28 Q A chart speed zero Rec on / off offup Q A UV 1 M NaOH Q A www.gelifesciences.co.jp/ bdm/purge.asp Tel03-5331-931506-6305-4707 18-1153-46 Upgrade Kit P-950,purge 50,000 Q A Q A Q 72 Change lamp or call service A UV UV 1 Q A 19-6067-02 Q no data A Q 62 Check that the tube position is OK A feed tube 30
Q Peak Integrate X A Peak Integrate X X Q RUN PrimeView A PC ÄKTAprime CheckOK Check communication. PC App. Connected! Check communication. Not Connected! PrimeView PC Q PrimeView Run Logbook A Properties Hide ViewWindow... Curves 71-2024-31p.15? FAQ Q A Q ÄKTAprime UV A UV UV Set Parameters LampOn LampOff UV UV 1 UV ÄKTAprime, ÄKTAprime plus FAQ Q 1.5ml A 12 mm 175 Eppendorf tube holder18-8522-01 Q PrimeView A 10 ManualRun 1 Q ÄKTAprime PrimeViewPC A PrimeView ÄKTAprime Q A PrimeViewPC PC ÄKTAprime PC ÄKTAprime RS-232C 1.8 m 5 m PC PC Tel 03-5331-9336 Q ÄKTAprime REC-112 PrimeView A ÄKTAprime ver.2.01 PrimeView PrimeView PC PrimeView ver.2.01 ÄKTAprime Tel03-5331-9336 Q ÄKTAprime PC REC-112 A PC REC-112 ÄKTAprime ver.2.01 ver.2.01 PC ÄKTAprime Q 31
A ÄKTAprime Template 600 40 Q ph A ph UV ph 88 µl PEEK 0.75 mm 10 cm 44.9 µl ph ph 4 2 M KNO3 =11 FAQ ÄKTAprime, ÄKTAprime plus Q A chart speed Q A 0.45 µm Q Template Result A Application Template Result AT Method Template MT Template Run Stored Method Result Application Template AT2003Aug1no1.RES Method Template MT2003Aug1no1.RES Run Stored Method 2003Aug1no1.RES Q PrimeView Evaluation Tile Tile A EditDelateChromatogram...Tile Q ÄKTAprime PC A PC ÄKTAprime 40 Copy Method PC 999 71-2024-31p.12 PrimeView ÄKTAprime PC PC PrimeView PC Copy ÄKTAprime PC Method 40 PC PrimeView UVConductivity PC (LogBook) PC Integrate Excel Export Laptop PC p.33 ÄKTAprime 32
ÄKTAprime Yes Yes No Method Yes ÄKTAprime No ÄKTAprime plus No No ÄKTAprime No Yes Yes 50,000 3 1 11-0012-77PrimeView 5 upgrade WinXP/Laptop 72-1146-03PRINTER FOR ÄKTA 72-0947-05OA-Tap 2 11-0012-77PrimeView 5 upgrade WinXP/Laptop 72-1146-03PRINTER FOR ÄKTA 72-0947-05OA-Tap 18-1153-46Upgrade Kit P-950, purge ÄKTAprime Pump Yes 3 18-1153-46Upgrade Kit P-950, purge No PrimeView 1 688,550 1 PrimeView 2 738,550 2 Method PC ÄKTAprime, ÄKTAprime plus ÄKTAdesign UV User Club p.18 p.19 p.19 p.20 p.36 p.36 2008 12 33
ÄKTAxpress, ÄKTAcrossflow ÄKTAxpress ÄKTAxpress, ÄKTAcrossflow 1 1 1 ÄKTAcrossflow 1 4 4 3 6! One Point 2010 mm NaOH 20 34
1 In/Out 2 2 ÄKTAcrossflow 3 4 5 In/Out 6 1 3 System Control System Calibrate 2 In/Out 2 4 Monitor ZeroLS Start calibrate 5 Start calibrate 6 In/Out 2 ph 1 System Control System Caribrate 2 ph ph 6. 88 Measured Value Reference Value1 ph Read value1 3 ph ph 4. 01 Measured Value Reference Value2 ph Read value2 4 Close 35
User Club ÄKTA User Club Handbook Gel Filtration Principles and Methods 2007 1 72-HB09-02 Handbook Gel Filtration Principles and Methods 2002 1 18-1022-18 Handbook Ion Exchange Chromatography & Chromatofocusing, Principles and Methods 2004 1 72-HB10-01 Handbook Ion Exchange Chromatography & Chromatofocusing, Principles and Methods 2004 1 11-0004-21 Handbook Affinity Chromatography Principles and Methods 2003 1 72-HB08-01 Handbook Affinity Chromatography Principles and Methods 2001 1 18-1022-29 Handbook Antibody Purification 2000 1 18-1037-46 Handbook The Recombinant Protein Purification 2006 1 18-1142-75 Handbook GST Gene Fusion System 2002 1 18-1157-58 Handbook GST Gene Fusion System 2002 1 72-HB07-01 Handbook Protein Purification 1998 1 18-1132-29 Protein PurificationPrinciples, High Resolution Methods, and Applications 1998 1 18-1128-68 STREAMLINE Handbook Expanded Bed AdsorptionPrinciples and Methods 1 72-0619-01 Hydrophobic Interaction and Reversed Phase Chromatography, Principles and Methods 2006 1 11-0012-69 Handbook Challenging Protein Purification 2007 1 28-9095-31 2008 1 72-HB13-01 Handbook 2003 1 72-HB05-02 Handbook 2006 1 72-HB06-03 Handbook 2005 1 72-HB12-02 User Club ÄKTA User Club ÄKTA PDF ÄKTA 1 /2 3 ÄKTA User Club BioDirect-JP@ge.com ÄKTA 36