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Shigella sonnei 200g 300 500cm 0.2 0.3mm 25g 0.45 m Shigella sonnei 3mL 35 37 18 ml 0.1 ml 10 10 ml 40mL 0.1 200 L 25g 1
225mL 35 37 18 24 Shigella broth 44 18 24 Shigella broth 15mL 35 37 18 24 Shigella broth 15mL 44 18 24 Buffered Peptone Water (OXOID,MERCK,, ) 10.0g 5.0g 4.0g* 1.5g* ph 7.2 0.1 1,000mL 121 15 40* Shigella broth,oxoid 20.0g 2.0g 2.0g 5.0g 2
1.0g Tween80 1.5mL 0.5mg 1000mL ph 7.0 0.2 31.5g/L 12115 50mg 0.2 m 225mL 2.5mL 25.0mg Tween80 1.08 1.5g/L OXOID,MERCK,BBL,, OXOID,BBL,, :Shigella sonnei 3
1 MAC MacConkey Agar No.3 OXOID,Difco,,, 20.0g 10.0g No.3 1.5g 5.0g 0.03g 0.001g 15.0g 1000mL ph 7.1 0.2 51.5g/L 12115 50 20mL CRT CHROMagarO157TAM CHROMagar 31.5g 12.0g 1.0g 1000mL ph 7.2 44.5g/L 50 20mL DHL Agar,,OXOID,MERCK, 3.0g 4
20.0g 15.0g 10.0g 1.0g 2.3g 1.0g 1.0g 0.03g 15.0g 1000mL ph 7.0 0.2 63.3g/L 50 20mL XLD Agar OXOID,,Difco,MERCK, 3.0g 7.5g 7.5g 3.75g 5.0g 1.0g 5.0g 6.8g 0.8g 0.08g 12.5g 1000mL ph 7.4 0.2 52.9g/L 50 20mL 5
Salmonella Agar acc. to Onoz MERCK 3.0g 6.0g 6.8g 11.5g 13.0g 3.825g 9.3g 4.25g L- 5.0g 0.5g 0.4g 0.00166g 0.022g 0.25g 0.47g 1.0g 15.0g 1000mL ph 7.1 0.2 80.5g/L 50 20mL HEA Hektoen Enteric Agar OXOID,Difco, 12.0g 3.0g 12.0g 12.0g 6
2.0g No.3 9.0g 5.0g 5.0g 1.5g 0.1g 0.065g 14.0g 1000mL ph 7.5 0.2 76.0g/L 50 20mL SS Agar Salmonella Shigella Agar OXOID MERCK Difco 5.0g 5.0g 10.0g 8.5g 8.5g 8.5g 1.0g 0.00033g 0.025g 13.5g 1000mL ph 7.0 0.2 60g/L 50 20mL 7
Shigella sonnei 35 37 18 24 35 37 18 24 Shigella sonnei 8
1m 1m 5g 75m 25mL OXOID No.714064-1 100 6,800 BBL No.261181 50 7,500 3 1m A LIM MERCK 12.8g 3.0g 1.0g L- 10.0g L- 0.5g 0.02g 2.7g 9
ph 6.8 0.1 30g/L 12115 B TSI OXOID,,,MERCK, 5.0g 5.0g 15.0g 10.0g 10.0g 1.0g 0.2g 0.2g 0.02g 15.0g ph 7.4 0.2 61.4g/L 12115 C, 5.0g 5.0g 5.0mg 0.5g L- 10.0g 0.02g 0.01g ph 6.0 0.1 10
60g/L 3m 12115 D,OXOID,MERCK,, 1.0g 1.0g 2.0g 0.2g 5.0g 0.024g 15.0g ph 6.7 0.1 24.2g/L 12115 E Acetate agar 2.0g 5.0g ( ) 0.2g 1.0g 1.0g 0.08g 20.0g 6.7 0.1 1,000mL 6.7 Acetate agar m 12115 5cm 11
Acetate agar E. colis.sonnei 35 3724 72 35 3724 72 7. 1949 Ewing 1984 Shigella dysenteriae Shigella flexneri Shigella boydii Shigella sonnei S.dysenteriae 12 S.flexneri 6 13 S.boydii 18 S.sonnei 1 35 3718 24 0.5m 30 5 10 12
3m 12115 10060 3000 10 8. PCR ipah InvE ipah 50% inve PCR DNA 1.5 m5,000 rpm 2 100 955 13,000 rpm 10 1.5 m 100 13
PCR TaKaRa Ex Taq TaKaRa Primer Set( inve ipah ) PCR TaKaRa Ex Taq (RR001) Primer Set TaKaRa inve Primer Set INV-1/2 (S016) TaKaRa ipah Primer Set IPA-1/2 (S017) X10 Ex Taq Buffer 2 dntp Mixture (2.5 mm each) 2 primer INV-1 0.2 primer INV-2 0.2 primer IPA-1 0.2 primer IPA-2 0.2 Ex Taq 0.1 14.1 Template DNA DNA * 1 * DNA DNA PCR 94 30 55 30 72 30 35 cycles 72 7 4 2% INV-1/2 Primer Set 293 bp IPA-1/2 Primer Set 242 bp DNA ipah inve 14