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- ゆうりゅう ひろなが
- 9 years ago
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1 PCR- (Code No. FIK-101) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4849K
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4 [2] 2x PCR Master Mix 1ml x Primer Mix 1ml 1-20 UNG(Uracil-DNA Glycosylase 200 l 1-20 [3] 100bp 1.5ml 0.2mlPCR 20ml 1.5ml 95 12,000rpm MultiNA MultiNA DNA-1000 SYBR Gold Code:DNA SYBR Molecular Probes Inc. [4] inva VT1, VT2, VT2vha, VT2vhb, VT2vp1 ipah ipah [5] PCR PCR 2
5 PCR PCR dttp dutp DNA UNG UNG dutp PCR dttp DNA UNG PCR UNG UNG UNG / ( /) ipah 10 4 inva VT 10 6 InvA VT ipah InvA VT ipah 3
6 [6] A, B A 1) 1.5ml 50μl 2) 1-2 3) 10μl 50 B 1) 20ml 2.5ml 2) 2-3 3) 2-3 4) 50 5) 2-3 6) 0.5ml 1.5ml 1) 1.5ml 95 5min 2) 12,000rpm X 3 3) 2μl PCR 4 PCR PCR 1) 2) 3) 4) 18μl 5.6 μl 2x PCR Master Mix 10.0 μl 10x Primer Mix 2.0 μl UNG 0.4 μl 2.0 μl 20.0 μl 4
7 PCR UNG : 20, 10 95, 2 : 94, 10 : 57, : 68, 30 : 94, 10 : 55, : 68, 30 : 94, 10 : 53, : 68, MultiNA PCR DNA 100bp DNA Ladder DNA MultiNA bp 5 mv bp 7 mv bp 2mV bp 2 mv 5
8 (1) 2 (2) [7] 6
9 [8] O O157 cfu/g) 0 7x10 2 7x10 3 7x10 4 7x10 5 7x10 6 PCR cfu/g) 0 7 x x x x x10 6 PCR cfu/g) 0 4 x x x x x10 6 PCR 50 O157 cfu/g) x10 2 7x10 3 7x10 4 7x10 2 7x10 3 7x x x x10 4 7
10 [9] 10 UV inva, VT, ipah 10 UV 8
11 9
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17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K - 18 - [1] 1 [2] 2 [3] 3 [4] 5 [5] : RNA cdna 13 [6] 14 [7] 17 LightCycler TM Idaho
(2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) 1 RT-PCR (10) (12) (13) F. Hoffmann-La Roche Ltd. Roche Molecular
05-8 SYBR Green Realtime PCR Master Mix (Code No. QPK-201, QPK-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3314K (2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux
(2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc.
06-08 SYBR Green Realtime PCR Master Mix -Plus- (Code No. QPK-211, 211T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN (2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene
(2) (2) (3) (3) (4) ABI PRISM 7700 TaqMan (4) Roche LightCycler TM (5) BioFlux LineGene TaqMan (6) 1 RT-PCR (7) (9) (10) F. Hoffmann-La Roche Ltd. Roc
05-8 Realtime PCR Master Mix (Code No. QPK-101, QPK-101T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3313K (2) (2) (3) (3) (4) ABI PRISM 7700 TaqMan (4) Roche LightCycler TM (5) BioFlux LineGene
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Marketplace Thermo Scientific ALL PCR DNA メ ー PCR 製品 dntp PCR ー ン キ Essentials Tour 2016 キャンペーン ー DNA ー 製キ 2016 年 7 月 1 日 2016 年 9 月 30 日 まで キャンペーンの はこちらをご覧ください www.thermofisher.com/jp-mktp PCR Thermo
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取扱説明書
19-02 One-step RT-PCR Kit RT-PCR Quick Master Mix (Code No. PCR-311F) 取扱説明書 TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3647K - 目次 - [1] はじめに 1 [2] 製品内容 2 [3] 使用方法 3 [4] 実施例 5 [5] 関連プロトコル 6 [6]
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[ ]...1 [ ] Inverse PCR...2 [ ]...3 [ ]...4 Plasmid PCR Primer...4 PCR...5 PCR 2nd-site mutation...5 Plasmid...6 [ ]...8 Inverse PCR Dpn I
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