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20128 364 20128 TEL03-6832-9300 2 70 1 GHSGlobal Harmonized System WEBwww.lifetechnologies.com/cartagena/ TEL0120-477-392 MSDSMaterial Safety Data Sheet MSDSwww.lifetechnologies.com TEL0120-477-392 DNAcDNA 1. 2. Life Technologies, Carlsbad, CA USA TEL03-6832-9350 i
209 211 213 214 217 218 208 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8
Cell Biology Catalog () CellTrace SYTOX Green nucleic acid stain CellTrace (AM) AM C3100MP20x50 μg CellTrace calcein probes Jurkat 1.0 μm CellTrace AM CellTrace AM 30180 Ex/Em 8 CellTrace calcein blue AM 360/440 20 50 μg C34853 56,000 15.2 CellTrace calcein green AM 494/517 20 50 μg C34852 55,400 15.2 CellTrace calcein red-orange AM 577/595 20 50 μg C34851 56,000 15.2 www.lifetechnologies.com 209
CellTracker GST CellTracker CellTracker CMFDA Leptohalysis scottijoan M. Bernhard(Wadsworth Center, New York State Department of Health) 24 CellTracker CellTrace CellTracker 1, 2 1. J Cell Biol 101, 610 (1985); 2. J Cell Biol 103, 2649 (1986). Ex/Em Thiol-reactive long-term cell tracers CellTracker Blue CMAC 353/466 5 mg C2110 39,900 10.1, 14.2 CellTracker Blue CMF2HC 371/464 5 mg C12881 39,900 10.1, 14.2 CellTracker Green CMFDA 492/517 1 mg C2925 50,200 14.2, 15.2, 15.6, 20.2 CellTracker Green CMFDA 492/517 20 50 μg C7025 59,600 14.2, 15.2, 15.6, 20.2 CellTracker Orange CMTMR 541/565 1 mg C2927 50,200 14.2 CellTracker Orange CMRA 548/576 20 50 μg C34551 59,600 14.2 CellTracker Red CMTPX 577/602 20 50 μg C34552 59,600 14.2 CellTracker Violet BMQC 415/516 5 100 μg C10094 27,200 14.2 Amine-reactive long-term cell tracers CFSE (CFDA-SE) 492/517 25 mg C1157 46,000 14.2, 15.4, 20.2 Vybrant CFDA SE Cell Tracer Kit 492/517 1 kit V12883 42,000 14.2, 15.4 CellTrace CFSE Cell Proliferation Kit* 492/517 1 kit C34554 30,000 14.2, 15.4 CellTrace Oregon Green 488 carboxylic acid diacetate, succinimidyl ester 492/518 20 50 μg C34555 62,000 14.2, 15.4 SNARF -1 carboxylic acid, acetate, succinimidyl ester 576/635 10 50 μg S22801 67,900 14.2, 15.4, 20.4 CellTrace Far Red DDAO-SE 646/659 20 50 μg C34553 60,000 14.2 * Optimized for tracing via flow cytometry. 210 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8
Cell Biology Catalog (C18)DiIDiD DiODiODiIDiDVybrant DiIDiODiD CM-DiIDiI DiICM-DiI Vybrant CM-DiINeuroTrace CM-DiI FAST DiI FAST DiO 1 FAST DiI 2 4 FAST DiO 5DiIDiO(C18 : 0)(C18 : 2) DiIDiO50% 1. Trends Neurosci 12, 333 (1989); 2. Neuroreport 8, 3965 (1997); 3. Biophys J 70, 988 (1996); 4. J Cell Biol 134, 1427 (1996); 5. Neuron 17, 91 (1996). NeuroTrace NeuroTrace DiODiICM-DiIDiDDiODiICM-DiIDiD NeuroTrace DiODiIDiD 50 80% DiI(DiIC 18 (3)- )DiD(DiIC 18 (5)- ) Ariel Agmon(West Virginia University) DilC(3)Dil Mount Holyoke Rachel Fink 8 www.lifetechnologies.com 211
Ex/Em Product Notes DiI and analogs DiIC18(3) DiI 549/565 Red-orange fluorescent lipophilic probe; widely used as a neuronal tracer 100 mg D282 54,400 12.4, 13.4, 14.4 DiIC18(3) DiI 549/565 Large-crystal form of DiI to facilitate direct application of crystals to membranes 25 mg D3911 42,900 13.4, 14.4 DiIC16(3) 549/565 Shorter-chain DiI analog that may incorporate into membranes more easily than DiI 100 mg D384 54,400 12.4, 13.4, 14.4 DiIC12(3) 549/565 Shorter-chain DiI analog that may incorporate into membranes more easily than DiI 100 mg D383 54,400 13.4, 14.4 FAST DiI oil 549/564 Unsaturated DiI analog that reportedly migrates ~50% faster than DiI within membranes 5 mg D3899 54,400 13.4, 14.4 FAST DiI Solid 549/564 Solid form to facilitate direct application of crystals to membranes 5 mg D7756 54,400 13.4, 14.4 Δ9-DiI oil 549/564 Unsaturated DiI analog that may migrate faster than DiI within membranes 25 mg D3886 54,400 13.4, 14.4 CellTracker CM-DiI 553/570 Chloromethylated DiI analog; potentially retained after fixation 20 50 μg and permeabilization C7000 50,200 13.4, 14.4 CellTracker CM-DiI 553/570 Chloromethylated DiI analog; potentially retained after fixation and permeabilization 1 mg C7001 43,900 13.4, 14.4 SP-DiIC18(3) 556/573 Anionic DiI analog with enhanced solubility in culture medium 5 mg D7777 54,400 13.4, 14.4 DiIC18(3)-DS 555/570 Anionic DiI analog with enhanced solubility in culture medium 5 mg D7776 54,400 13.4, 14.4 DiD and analogs DiIC18(5) oil DiD 644/665 Useful in autofluorescent samples and as a second tracer in combination with DiI 25 mg D307 36,700 13.4, 14.4 DiIC18(5) DiD 644/663 Solid form to facilitate direct application of crystals to membranes 10 mg D7757 29,400 13.4, 14.4 DiIC18(5)-DS 650/670 Anionic DiD analog with enhanced solubility in culture medium 5 mg D12730 54,400 13.4, 14.4 DiR DiIC18(7) DiR 748/780 Absorption and emission in the near-infrared region, where many tissues are optically transparent 10 mg D12731 39,900 13.4, 14.4 DiO and analogs DiOC18(3) DiO 484/501 Widely used as a second tracer in combination with DiI 100 mg D275 54,400 13.4, 14.4 DiOC16(3) 484/501 Shorter-chain DiO analog that may incorporate into membranes more easily than DiO 25 mg D1125 54,400 13.4, 14.4 FAST DiO Solid 484/499 Unsaturated DiO analog that reportedly migrates ~50% faster than DiO within membranes 5 mg D3898 54,400 13.4, 14.4 SP-DiOC18(3) 497/513 Anionic DiO analog with enhanced water solubility 5 mg D7778 54,400 13.4, 14.4 DiA and analogs 4-Di-16-ASP DiA 480/540* Useful as a second tracer with DiI 25 mg D3883 46,100 13.4, 14.4 FAST DiA Solid 480/540* Solid form to facilitate direct application of crystals to membranes 5 mg D7758 54,400 13.4, 14.4 4-Di-10-ASP 480/540* Shorter-chain DiA analog that may incorporate into membranes more easily than DiA 25 mg D291 46,100 13.4, 14.4 NeuroTrace tissue labeling pastes NeuroTrace DiI Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 549/565 the tip of a needle 500 mg N22880 32,600 14.4 NeuroTrace CM-DiI Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 549/565 the tip of a needle 100 mg N22883 32,600 14.4 NeuroTrace DiO Can be applied directly to live or fixed tissue specimens using tissue-labeling paste 484/501 the tip of a needle 500 mg N22881 32,600 14.4 NeuroTrace Multicolor Contains 500 mg of DiO, DiI, and DiD labeling pastes for Tissue-Labeling Kit multiple multicolor tracing 1 kit N22884 77,200 14.4 * Optimized for tracing via flow cytometry. 212 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8
Cell Biology Catalog 1 Alexa Fluor (8%)200 mm KClAlexa Fluor - 3 (DAB)(photoconvert) 2 3100 mm 3 (--L-) 4 5 Alexa Fluor 1. Methods Cell Biol 29, 153 (1989); 2. J Neurosci Methods 41, 45 (1992); 3. J Neurosci Methods 33, 207 (1990); 4. Nature 377, 734 (1995); 5. J Neurosci 14, 4338 (1994). 8 CH101 Aplysia californica (J. Neurophysiol 66, 316 (1991)) David Kleinfeld (AT & T Bell Laboratories) Brian Salzberg (University of Pennsylvania School of Medicine) Alexa Fluor 568()Alexa Fluor 488 ()Alexa Fluor 488Alexa Fluor 568 ()3 Michiel van Wyk(University of Queensland, Australia) www.lifetechnologies.com 213
Ex/Em Hydrazide derivatives (fixable) Alexa Fluor 350 345/445 5 mg A10439 36,600 3.3, 14.3 Alexa Fluor 488 493/517 1 mg A10436 36,600 3.3, 14.3 Alexa Fluor 555 554/567 1 mg A20501MP 36,600 3.3, 14.3 Alexa Fluor 568 576/599 1 mg A10437 36,600 3.3, 14.3 Alexa Fluor 568 576/599 125 μl* A10441 34,400 14.3 Alexa Fluor 594 588/613 1 mg A10438 36,600 3.3, 14.3 Alexa Fluor 594 588/613 125 μl* A10442 34,400 14.3 Alexa Fluor 633 624/643 1 mg A30634 37,000 3.3, 14.3 Alexa Fluor 647 649/666 1 mg A20502 36,600 3.3, 14.3 Lucifer yellow CH (lithium salt) 428/536 25 mg L453 19,100 3.3, 14.3, 16.1 Lucifer yellow CH (lithium salt) 428/536 100 μl* L12926 37,700 14.3 Lucifer yellow CH (potassium salt) 428/536 25 mg L1177 19,100 14.3 Biocytin derivatives (fixable) Alexa Fluor 488 494/520 250 μg A12924 36,600 4.3, 14.3 Alexa Fluor 546 556/572 250 μg A12923 42,300 4.3, 14.3 Alexa Fluor 594 591/618 250 μg A12922 42,300 4.3, 14.3 Biocytin NA 100 mg B1592 22,400 4.2, 14.3 Other polar tracers (nonfixable) Aminostilbamadine 361/536 10 mg A22850 33,600 14.3 hydroxystilbamidine 345/450 10 mg H22845 33,600 8.1, 14.3 Sulforhodamine 101 586/605 25 mg S359 20,100 14.3, 16.1 True blue chloride 375/403 5 mg T1323 39,900 14.3 * For microinjection. -(-D-1,6-) 1 3 in vivo Vybrant Alexa Fluor 546 4, 5 Net charge FITC Alexa Fluor 488 Alexa Fluor 488 Alexa Fluor B 1. Nature 344, 431 (1990); 2. Science 252, 569 (1991); 3. Development 108, 581 (1990); 4. Brain Res 526, 127 (1990); 5. Brain Res Bull 25, 139 (1990). 214 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8
Cell Biology Catalog 32 Xenopus laevis31a1 10,000 MW2B1 3C3Texas RED 10,000 MWCascade Blue 10,000 MW1 10 Marie Vodicka Fixable Common Name 3,000 MW dextran conjugates 3000 MW Texas Red Gambusia affinis Bipinnate inclinator muscles Texas Red University of Nebraska Medical CenterE. Rosa-MolinarCreightonBernd Fritzsch Alexa Fluor 488 No 2 mg D34682 24,300 14.5 Alexa Fluor 680 No 2 mg D34681 24,300 14.5 Amino Yes 100 mg D3330 22,200 14.5 Biotin Yes BDA-3000 10 mg D7135 50,200 4.3, 14.5 Cascade Blue Yes 10 mg D7132 50,200 14.5 Fluorescein No 10 mg D3305 43,900 14.5, 20.4 Fluorescein Yes 10 mg D3306 50,200 14.5 Fluorescein and biotin Yes micro-emerald 5 mg D7156 59,600 4.3, 14.5 Rhodamine Green No 5 mg D7163 51,300 14.5 Tetramethylrhodamine No 10 mg D3307 43,900 14.5 Tetramethylrhodamine Yes 10 mg D3308 50,200 14.5 Tetramethylrhodamine and biotin Yes micro-ruby 5 mg D7162 59,600 4.3, 14.5 Texas Red No 10 mg D3329 43,900 14.5 Texas Red Yes 10 mg D3328 50,200 14.5 10,000 MW dextran conjugates Alexa Fluor 488 Yes 5 mg D22910 59,200 14.5 Alexa Fluor 546 Yes 5 mg D22911 59,200 14.5 Alexa Fluor 555 Yes 5 mg D34679 59,200 14.5 Alexa Fluor 555 Yes 5 mg D22913 59,200 14.5 Alexa Fluor 568 Yes 5 mg D22912 59,200 14.5 Alexa Fluor 647 Yes 2 mg D22914 32,200 14.5 Alexa Fluor 680 Yes 5 mg D34680 59,200 14.5 Amino Yes 1 g D1860 30,500 14.5 8 www.lifetechnologies.com 215
Fixable Common Name Biotin Yes BDA-10,000 25 mg D1956 43,900 4.3, 14.5 Biotin Yes NeuroTrace BDA-10,000 Neuronal Tracer Kit kit N7167 59,600 14.5 BODIPY FL Yes 5 mg D7168 43,900 14.5 Cascade Blue Yes 25 mg D1976 43,900 14.5 Fluorescein No 25 mg D1821 32,600 14.5, 20.4 Fluorescein Yes fluoro-emerald 25 mg D1820 43,900 14.5 Fluorescein and biotin Yes mini-emerald 10 mg D7178 59,600 4.3, 14.5 Lucifer yellow Yes 25 mg D1825 32,600 14.5 Oregon Green 488 No 5 mg D7170 43,900 14.5, 20.4 Oregon Green 488 Yes 5 mg D7171 50,200 14.5 Rhodamine B No 25 mg D1824 32,600 14.5 Rhodamine Green Yes 10 mg D7153 59,600 14.5 Tetramethylrhodamine No 25 mg D1816 32,600 14.5 Tetramethylrhodamine Yes 25 mg D1868 32,600 14.5 Tetramethylrhodamine Yes fluoro-ruby 25 mg D1817 43,900 14.5 Tetramethylrhodamine and biotin Yes mini-ruby 10 mg D3312 59,600 4.3, 14.5 Texas Red No 25 mg D1828 32,600 14.5 Texas Red Yes 25 mg D1863 43,900 14.5 40,000 MW dextran conjugates Amino Yes 1 g D1861 30,500 14.5 Fluorescein No 25 mg D1844 32,600 14.5, 20.4 Fluorescein Yes 25 mg D1845 43,900 14.5 Tetramethylrhodamine No 25 mg D1842 32,600 14.5 Texas Red No 25 mg D1829 32,600 14.5 70,000 MW dextran conjugates Amino Yes 1 g D1862 30,500 14.5 Biotin Yes BDA-70,000 25 mg D1957 43,900 4.3, 14.5 Fluorescein No 25 mg D1823 32,600 14.5, 20.4 Fluorescein Yes 25 mg D1822 43,900 14.5 Oregon Green 488 No 5 mg D7172 43,900 14.5, 20.4 Oregon Green 488 Yes 5 mg D7173 50,200 14.5 Oregon Green 514 No 5 mg D7176 43,900 14.5, 20.4 Rhodamine B No 25 mg D1841 32,600 14.5 Tetramethylrhodamine No 25 mg D1819 32,600 14.5 Tetramethylrhodamine Yes 25 mg D1818 43,900 14.5 Texas Red No 25 mg D1830 32,600 14.5 Texas Red Yes 25 mg D1864 43,900 14.5 500,000 MW dextran conjugates Amino Yes 100 mg D7144 22,200 14.5 Biotin Yes BDA-500,000 10 mg D7142 43,900 4.3, 14.5 Fluorescein Yes 10 mg D7136 43,900 14.5 2,000,000 MW dextran conjugates Fluorescein Yes 10 mg D7137 43,900 14.5 Tetramethylrhodamine Yes 10 mg D7139 43,900 14.5 216 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8
Cell Biology Catalog BCTBCTB BAAlexa Fluor Bovine serum albumin (BSA) conjugates Alexa Fluor 488 5 mg A13100 39,400 14.7 Alexa Fluor 555 5 mg A34786 39,400 14.7 Alexa Fluor 594 5 mg A13101 39,400 14.7 Alexa Fluor 647 5 mg A34785 39,400 14.7 Alexa Fluor 680 5 mg A34787 39,400 14.7 BODIPY FL 5 mg A2750 21,200 14.7 DNP 5 mg A23018 21,200 14.7 Fluorescein 5 mg A23015 21,200 14.7 Tetramethylrhodamine 5 mg A23016 21,200 14.7 Texas Red 5 mg A23017 21,200 14.7 Ovalbumin conjugates Alexa Fluor 488 2 mg O34781 20,700 14.7 Alexa Fluor 555 2 mg O34782 20,700 14.7 Alexa Fluor 594 2 mg O34783 20,700 14.7 Alexa Fluor 647 2 mg O34784 20,700 14.7 Fluorescein 5 mg O23020 21,200 14.7 Texas Red 5 mg O23021 21,200 14.7 Cholera toxin subunit B conjugates Alexa Fluor 488 100 μg C34775 36,300 7.7, 14.7, 16.1 500 μg C22841 115,600 7.7, 14.7, 16.1 Alexa Fluor 555 100 μg C34776 36,300 7.7, 14.7, 16.1 500 μg C22843 115,600 7.7, 14.7, 16.1 Alexa Fluor 594 100 μg C34777 36,300 7.7, 14.7, 16.1 500 μg C22842 115,600 7.7, 14.7, 16.1 Alexa Fluor 647 100 μg C34778 36,300 7.7, 14.7, 16.1 Biotin-XX 100 μg C34779 36,700 4.3, 7.7, 14.7, 16.1 Horseradish peroxidase 100 μg C34780 36,700 7.7, 14.7, 16.1 Other protein conjugates Protein Histone H1 (calf thymus) Alexa Fluor 488 1 mg H13188 31,200 12.5, 16.1 Parvalbumin (codfish) Alexa Fluor 488 1 mg P23012 32,200 14.7 Trypsin inhibitor (soybean) Alexa Fluor 488 1 mg T23011 26,900 14.7, 16.1 SAIVI injectable contrast reagents Bovine serum albumin Alexa Fluor 680 1 ml S34788 76,800 14.7 Human serum transferrin Alexa Fluor 680 1 ml S34790 101,700 14.7 Alexa Fluor 750 1 ml S34791 101,700 14.7 8 www.lifetechnologies.com 217
NeuroTrace Franz Nissl100 -O O 5 FluoroMyelin NeuroTrace 530/615 DAPI DAPIBrainStain MBPLoyezSchmued's 20 HuC/HuD embryonic lethal abnormal visual(elav) RNAHuDHuCHel-N1 HuR FluoroMyelin Green FluoroMyelin RedNeuroTrace DAPI NeuroTrace fluorescent Nissl stains Color Ex/Em Blue 435/455 1 ml N21479 32,600 8.1, 12.5, 14.3 Green 500/525 1 ml N21480 32,600 8.1, 12.5, 14.3 Yellow 515/535 1 ml N21481 32,600 8.1, 12.5, 14.3 Red 530/615 1 ml N21482 32,600 8.1, 12.5, 14.3 Deep red 640/660 1 ml N21483 32,600 8.1, 12.5, 14.3 FluoroMyelin myelin stains Green 479/598 1 ml F34651 43,900 14.4 Red 558/654 1 ml F34652 43,900 14.4 BrainStain Imaging Kit Contents Contains FluoroMyelin Green, NeuroTrace 530/615 red Nissl stain, and DAPI B34650 80,300 14.4 218 Important Licensing Information: The products in this catalog may covered by one or more Limited Use Label Licenses (see the Life Technologies website www.lifetechnologies.com). By use of these products, you accept the terms and conditions of all applicable Limited Use Label Licenses. All products are for research use only, and are not intended for human or animal diagnostics, therapeutic, commercial, or other clinical uses unless otherwise specifically indicated in the applicable product Limited Use Label License(s). 2012 8