COLAK Mehmed
11000 305 305 180 10000 9000 8000 160 140 120 7000 100 6000 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 2003 2004 2005 80
GH IGF-1
GH GH
5 4 3 2 1 0 2 3 4 5 6 7 8 9 10 11
Kawashima et al., J Reprod Dev, 52 (2006)
1 Kawashima et al., J Dairy Sci, 90 (2007)
Kawashima et al., J Dairy Sci, 90 (2007)
Kawashima et al., J Dairy Sci, 90 (2007)
55 50 75 70 65 P<0.001 vs. 45 60 40 55 50 35 45 30 6 9 12 15 18 21 24 27 30 40-4 -3-2 -1 0 1 2 3 Kawashima et al., Reproduction, 133 (2007)
140 120 100 80 P<0.05 P<0.001 25 20 15 P<0.01 P<0.001 60 40 20 10 5 0 0 Kawashima et al., Reproduction, 133 (2007)
35 1000 30 900 800 25 700 20 15 600 500 400 10 5 0 P<0.0001 vs. 1 3 5 7 9 11 13 15 17 19 21 300 200 100 0 P<0.0001 vs. 1 3 5 7 9 11 13 15 17 19 21 Kawashima et al., J Reprod Dev, 53 (2007)
250 200 150 100 50 1 3 5 7 9 11 13 15 17 19 21 P<0.0001 vs. Kawashima et al., J Reprod Dev, 53 (2007)
IGF (Insulin-like growth factor), IGF-I IGF-II (Type I II) (1-6)
1 2 3 4 E2
Kawashima et al., Reproduction, 133 (2007)
18 15 8 6 P<0.05 12 4 9 2 6 0 1 1.5 2 1 1.5 2 Kawashima et al., Reproduction, 133 (2007)
0.8 n=5 0.8 n=4 0.6 0.4 0.2 0 250 200 150 100 50 0 12 10 8 6 4 2 0-10 -8-6 -4-2 0 2 4 6 8 E2 0.6 0.4 0.2 0 250 200 150 100 50 0 12 10 8 6 4 2 0 10 11 12 13 14 15 16 17 18 19 20 Kawashima et al., Reproduction, 133 (2007)
Exp.1. ; (1) 1 1 cm 2 mm Aloka SSD-5500 1 cm 11
Exp.2. LH ; (1) GnRH 1 cm Pre GnRH GnRH 0.5 h 1 h 2 h 6 h 12 h 24 h 30 h 48 h Acosta et al., Reproduction, 125 (2003)
Exp.2. LH ; (1) 1 cm -30 h -24 h -18 h -6 h 0 h LH 6 h 12 h 24 h Acosta et al., Reproduction, 125 (2003)
Exp.2. LH ; (1) 42
GH
LH (E2) FSH LH FSH LH
LH LH P4 P4 P4 P4
FSH LH E2 P4 GH IGF-1?? 5
LH >8 mm 1 P4 2 PGF2α 2 42 h 1 LH P4 P4 39
PGF2α 42h 3 6
VS. (P4 E2) LH GH IGF-1 2 40
12 mean SEM F1 F2 F3 F4 12 mean SEM (mm) 10 8 6 (mm) 10 8 6 4-1 0 1 2 4-1 0 1 2 Day 1 F1 F2, F3, F4 (P<0.05) Day 1 F1, F2 F3, F4 (P<0.05) 41
1 P4 (ng/ml) 3 2 1 0 P4 mean SEM *: P<0.05 **: P<0.01 ***: P<0.001 Between two groups * * * * ** *** E2 (pg/ml) 10 8 6 4 2 E2 mean SEM *: P<0.05 Between two groups * 0 0.6-1 0 1 2-1 0 1 2-1 0 1 2 * * * FSH (ng/ml) 1.4 1.2 1.0 0.8 FSH mean SEM 2 42
LH 1 F1 F1 F2 F3 F2 GH 51
mrna expression (x10 4 ) 30 20 10 0 mean SEM a,b: P<0.05 a LH x b y c z mrna expresion (x10 4 ) 3 2 1 mean SEM a,b: P<0.05 x,y: P<0.05 a x 0 GH b y b xy
1 IGF-1 180 mean SEM a,b: P<0.05 a 1.5 mean SEM IGF-1 (ng/ml) 140 100 b b b ab ab ab ab Insulin (ng/ml) 1.0 0.5 60-1 0 1 2 0-1 0 1 2 Day 2 GH???? 47
GH LH IGF-1 2 F1 F2 F1 F2 E2 LH P4 P4 LH 2 P4 E2 LH GH GH IGF-1 2 51
: 16 10 6 12 : 2 40 50 100 EIA : P4 GH IGF-1
P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) 10 8 6 4 0.9 0.8 0.7 0.6 0.5 0.4 95 75 55 35 5 4 3 2 1 0 ab a ab ab ab ab ab b a cd bc ab bcd cd cd d a ab bc ab cd de e de -0.5 0 0.5 1 1.5 2 2.5 3 P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) 8 7 6 5 4 3 0.6 0.4 0.2 150 130 110 a b b b b b 90 70 50 5 ab 4 ab 3 abc 2 1 d cd 0-0.5 0 0.5 1 1.5 2 2.5 3 b b a abc bcd Kawashima et al., J Reprod Dev, 53 (2007)
E2 LH GH P4?? IGF-1
GH LH E2 P4?? LH GH IGF-1 5
Mehmet ÇOLAK
Growth hormone GH Insulin? Steroids IGF-1 Insulin-like growth factor-1
Control (n=3) P4 (n=4) 2 CIDR BIOPSY SLAUGHTERHOUSE EB inj IM (1 mg) BIOPSY SLAUGHTERHOUSE E2 (n=3) BIOPSY SLAUGHTERHOUSE -2-1 0 1 2 3 4 5 6 7 Serial Blood Collection 15 min, 16 h Daily Blood Collection
Analysis of Plasma E2, P4, GH, IGF-I and Insulin Hormone analyses were performed by EIA Analysis of GHR, IGFR-I and IR genes Quantitative PCR method using BioRad
P4 (ng/ml) 8 6 4 2 *** *** *** *** *** *** *** Mean S.E.M. ***:P<0.001 0 E2 (pg/ml) 60 50 40 30 20 10 0 *** *** -2-1 0 1 2 3 4 5 6 7 DAY
Control 60 40 20 GH (ng/ml) P4 group E2 group 0 60 40 20 0 60 40 20 0 0 2 4 6 8 10 12 14 16 Time (h)
(ng/ml/min) 25000 20000 15000 10000 5000 0 a b c Mean S.E.M. a, b, c: P<0.05 (ng/ml) 20 15 10 5 a b a PULSAR program 0 (16 h) 8 6 4 2 0 b a a Control E2 P4
140 Control P4 E2 * Mean S.E.M. *:P<0.05 120 IGF-I (ng/ml) 100 80 60 40 20 0 Before After Before After Before After
Total GH-R P=0.055 mrna ( 10-7 ) Insulin-R mrna IGF-R1 mrna ( 10-6 ) b a a Control E2 P4 b a a Mean S.E.M. a, b: P<0.05
Growth hormone GH E2 IGF-I GHR IGFR-I IR
Estradiol+Progesterone Eimeria bovis 120 100 * * * * * * * * Percentage Feed Intake 80 60 40 20 0 15 16 17 18 19 20 21 22 23 24 25 26 27 28 Day Heath et al, Am J Vet Res (1997)
Estrogen+Proegesterone, but not GH 30 20 *a a *a *a *a Weight Gain (kg) 10 0-10 -20-30 Con EP GH PF C EP\C GH\C * a Heath et al, Am J Vet Res (1997)
350 Duration (min) 300 250 200 150 100 50 0 * * * *,* * C: control E: endotoxin S: Synovex S (P/E) C/E Saliva S/E Saliva C/E Pant S/E Pant C/E Cough S/E Cough McMahon et al, J. Endocrinol., (1998)
Growth hormone GH E2 IGF-I GHR
Growth hormone GH E2 P4 GHR IGF-I GHIGF-I E2/P4
E/P
IGF IGF-1 IGF-1
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