ニュースレター10-1.indd

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1 The Japanese Society of Immunotoxicology aaaa Tel / Dr. R.H. Pieters () "Immunotoxicology of therapeutics associated with allergy and autoimmunity" 2005 <> In Vitro Immunotoxicology 4/13 / THP- () () :00 /5,000 7,000 /3,000 4,000 /8,00010,000 5,0006, Fax: Tel: jsit-sec@pharm.teikyo-u.ac.jp 1

2 B Allergen Database for Food Safety; ADFS 1 PDBHSSP ID FAO/WHO2001 FAO/WHO CTC List of Allergens in Swiss-ProtAllergen NomenclatureThe Biotechnology Information for Food Safety DatabaseSDAPID cdna TrEMBL Structural Database of Allergenic Proteins SDAPSource Entrez PubMed 32SDAP OSSolaris 9 MySQL 4.0SRS SRS 7.1.3UniProt protein-protein BLASTblatp BLAST Search for short, nearly exact matches FAO/WHO 2 Hileman Allergen Database for Food Safety; ADFS BLAST FAO/WHO FAO/WHO N 80 FASTA 35% 2 ADFSHileman 3 FASTA overlap 80 2

3 Vol. 10 No URL Allergen Database for Food Safety (ADFS) FAO/WHO *1 Allergen Nomenclature 1, Structural Database of Allergenic Proteins (SDAP) List of Allergens in Swiss-Prot Allergome 1, CSL the Allergen Database ~ Food Allergy Research and Resource Program (Farrp) 1, *2 Allermatch.org tm Bioinformatics for Food Safety (BIFS) *1 Hileman 3 * % E-value ADFS Allergen Nomenclature International Union of Immunological SocietiesIUIS 1,144 Allergome Structural Database of Allergenic ProteinsSDAP ADFS ADFS ADFS ADFS 1) URL : 2) Report of a Joint FAO/WHO Expert Consultation on Foods Derived from Biotechnology, (2001) URL : 3) Hileman, R.E., Silvanovich, A., Goodman, R.E., Rice, E.A., Holleschak, G., Astwood, J.D., Hefle, S.L.: Int. Arch. Allergy Immunol., 128, (2002) 3

4 26Munich Munich T 1520 Messestadt WestICM Norway polystyrene particlesova IgE 60 OVATh2 Th1 11 5Q33 T-cell immunoglobulin mucintim TIMA Th2 TIM1A ATIM1 Th2 Th1TIM3EAE Th1 TIM3 TIM1 primary biological aerosol particles PBAP pollen-associated lipid mediatorpalm IL-12Th2 PBAPs PALM DNA HPA 4

5 Vol. 10 No nerve growth factor-inducible protein A GSF HPA FA HPA OVA HPA 5

6 FAHPA ppb 12 RT-PCR FA CRH-FA a CRH ACTH- ACTH mrnacrh- ACTH- 80 ppb400 ppb 2000 ppb FAHPA 2000 ppb FA OVA CRH- FA80 ppb 400 ppb 2000 ppb bacthmrna 80 ppbfa FACRH ACTH HPA FAHPA 80 ppb FA CD3CD4CD8CD19 CD4/CD8FA Th2 10 FAHPA FA HPA FAHPA HPA 6

7 Vol. 10 No (2) 1999 CFU-GM Assay ELISA NK SRBC PFC (2) 9 T cell Maximization Test 9 ()

8 21 Local Lymph Node Assay Popliteal Lymph Node Assay (PLNA) PCR RT-PCR MCP-1 RT-PCR () NK (IgM, IgG, IgA) Affymetrix GeneChip 12 8

9 Vol. 10 No DiOC18 NK Immunotoxicology Immunotoxicology () Immunotoxicology Immunotoxicology ICH Society of Toxicology 42 nd Annual Meeting

10 Analytical stage Mechanism-based stage ICHS Immunotoxicology Immunotoxicology Asbestos LLNA-DA ICH 8 IgE Non-RI Local lymph node assay (Non-RI LLNA) BrdU

11 Vol. 10 No CFU-GM Assay ELISA NK 7 SRBC PFC Maximization Test Local Lymph Node Assay Popliteal Lymph Node Assay (PLNA) PCR RT-PCR MCP-1 RT-PCR (IgM, IgG, IgA) Affymetrix GeneChip DiOC18 NK LLNA-DA Non-RI Local lymph node assay (Non-RI LLNA) BrdU () ()

12 ImmunoTox Letter Letter H.F 12

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