MM_AF_09_04.indd

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1 ライカ MM AF powered by MetaMorph Living up to Life

2 蛍光バイオイメージングシステム MM AF MetaMorph MM AF / / MM AF 3D 3D FRET FISH MM AF 3D MM AF DMI6000 B 2

3 ライカ MM AF MM AF CCD DFC SuperZ Galvo PIFOC I/O MM AF MM AF 2 MM AF DFC MM AF Offline MM AF SuperZ Galvo DFC360 FX 3

MM AF 2 XY Z Z パワフルな多次元イメージングツール MM AF XYZ MM AF QuickTime AVI 3D Z 2D 3D 3D 3D Continuous T cell receptor signaling required for synapse maintenance and full effector potential Johannes B.

4 MM AF 2 XY Z Z パワフルな多次元イメージングツール MM AF XYZ MM AF QuickTime AVI 3D Z 2D 3D 3D 3D Continuous T cell receptor signaling required for synapse maintenance and full effector potential Johannes B. Huppa 1,2, Michael Gleimer 1, Cenk Sumen 1,3 and Mark M. Davis 1,2 1Stanford University School of Medicine, Department of Microbiology and Immunology 2Howard Hughes Medical Institute, Stanford, CA Center for Blood Research, Harvard Medical School, Boston, MA Figure 1 PI3K TCR-CD3 TCR 5c.c7 TCR T PH (AKT)-YFP CD3 -CFP 2 6 T 15% CH27 B MCC 0.4 M T (a) DIC (b d) PH(AKT)-YFP (b) CD3 -CFP (c) (d) (e, f) 3D PH (AKT)-YFP (e) CD3 -CFP (f) (g) 16 a PH (AKT)- YFP CD3 -CFP a PH (AKT)-YFP high-low CD3 -CFP Merge Reprinted with permission from Nature Immunology (2003) 4: c

5 経時変化の観察 FRET MM AF streaming streaming Live Replay EL EL ms EL6000 FluoCells #2 F BODIPY FL IgG F- Texas Red -X DAPI 5

コローカリゼーションと輝度解析 MM AF 2 / MM AF MM AF Leila Tarsa and Yukiko Goda Division of Biology, University of California at San Diego, La Jolla, CA 92093-0366 a Figure 2 syp/syt syp 12 A A B syp syt syp/ syt

6 コローカリゼーションと輝度解析 MM AF 2 / MM AF MM AF Leila Tarsa and Yukiko Goda Division of Biology, University of California at San Diego, La Jolla, CA a Figure 2 syp/syt syp 12 A A B syp syt syp/ syt = 20 m A 5 m B syp syt syp syt 3% Leila Tarsa and Yukiko Goda (2002) Synaptophysin regulates activity-dependent synapse formation in cultured hippocampal neurons. PNAS. 99(2): c 2003 National Academy of Sciences, U.S.A. b 6

7 粒子トラッキングとモーション解析 MM AF point-to-point X/Y Wendy C. Salmon, Michael C. Adams, and Clare M. Waterman-Storer, Department of Cell Biology and Institute for Childhood and Neglected Diseases, The Scripps Research Institute, La Jolla, CA Figure 3 leading edge MT F-actin (a) 3 content/full/ jcb / DC1 Cy2 MT Xrhodamine F-actin Kymograph b-elp lamellum la cz cb 3 MT (b-e) a 2 Kymograph MT Actin = 10 m Reproduced from The Journal of Cell Biology, 2002, 158(1), by copyright permission of The Rockfeller University Press. 7

蛍光で要求されるスピードと正確さ MM AF CCD MM AF FRET MM AF FRET Ran-GTP Petr Kalab, Karsten Weis, and Rebecca Heald, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720-3200

8 蛍光で要求されるスピードと正確さ MM AF CCD MM AF FRET MM AF FRET Ran-GTP Petr Kalab, Karsten Weis, and Rebecca Heald, Department of Molecular and Cell Biology, University of California, Berkeley, CA Figure 4 Ran Ran-GTP 10 m (A) MT IYFP ICFP FRET IFRET/ICFP MT-FRET MT-FRET YRC FRET YIC Ran-GTP FRET FRET ICFP Reprinted with permission from Science (2002) 295(5564): c 2003 American Association for the Advancement of Science. 8

カウント分類測定多重細胞解析パラメーター MM AF / / MM AF 100 4 & Waltraud G. Muller, Dawn Walker, Gordon L. Hager, and James G.

9 カウント分類測定多重細胞解析パラメーター MM AF / / MM AF & Waltraud G. Muller, Dawn Walker, Gordon L. Hager, and James G. McNally, Laboratory of Receptor Biology and Gene Expression, National Cancer Institute, National Institutes of Health, Bethesda, MD Figure 5 a f 100 nm 3 GFP-GR RNA FISH a f RNA FISH 113 a-f 1 RNA FISH 1 m Reproduced from The Journal of Cell Biology, 2001, 154(1), by copyright permission of The Rockfeller University Press. 9

技術情報 MM AF / DFC XY SuperZ Galvo EL6000 1 3rd Party CCD & EM

stitching & MM AF / Mulitiwavelength Scoring 7 1 m U2OS

Ser28Transfluor Vesicles mouse anti-beta tubulin primary

10 技術情報 MM AF / DFC XY SuperZ Galvo EL rd Party CCD & EM CCD & Z Scan Slide Live Replay 3D 4D visualization & 3 Image stitching & MM AF / Mulitiwavelength Scoring 7 1 m U2OS 2-RrGFP Hoechst Anti-Phospho- Histone H3 Ser28Transfluor Vesicles mouse anti-beta tubulin primary antibody FITC FITC conjugated goat antimouse secondary antibody CHO-K1 Hoechst

11 & & & & QuickTime & AVI IMA Integrated Morphometry Analysis Nearest neighbors & No neighbors 3D 2D FRET & Z Kymograph MM Neurite Outgrowth MM Angiogenesis MM Count Nuclei and Cell Scoring / MM Multi Wavelength Cell Scoring MM Cell Cycle MM Cell Health MM Granularity MM Live Dead MM Mitotic Index MM Monopole Detection MM AF DM6000 MM AF / 11

12 ライカマイクロシステムズ株式会社本社大阪セールスオフィス名古屋セールスオフィス福岡セールスオフィス東京都港区白金白金高輪ステーションビル 6F 大阪市北区豊崎商業第 2 ビル 10F 名古屋市中区錦三永伏見ビル 2F 福岡市博多区店屋町 8-30 博多フコク生命ビル 12F marketing@leica-microsystems.co.jp この仕様は改良のため予告なく変更する場合があります Tel Fax Tel Fax Tel Fax Tel Fax Cat. No. MMAF_B_YU090450

第84回日本遺伝学会-抄録集.indd

第84回日本遺伝学会-抄録集.indd A S1 9 24 14 00 17 00 Epigenomic regulation of cell fate determination and homeostasis Organizers: (National Institute of GeneticsKyushu University 14 00 S1-1 1 RIKEN BioResource Center 2 Graduate School