(2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc.

Size: px

Start display at page:

Download "(2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc."

たしろうかやぬま
5 years ago
Views:

1 06-08 SYBR Green Realtime PCR Master Mix -Plus- (Code No. QPK-211, 211T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN

2 (2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) (10) (12) F. Hoffmann-La Roche Ltd. Roche Molecular Systems Inc. The Perkin-Elmer Corporation Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process for The Research Field in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Perkin-Elmer or as purchased, i.e., an authorized thermal cycler. LightCycler TM Idaho Technology Inc. Roche Molecular Systems Inc. SYBR Molecular Probes Inc. ABI PRISM The Perkin-Elmer Corporation

3 PCR SYBR Green Realtime PCR Master Mix -Plus- dntp PCR SYBR Green I DNA PCR LightCycler TM BSA ABI PRISM 7700 LightCycler TM LineGene Taq PCR 10 1 LightCycler TM PCR

4 (QPK ) [SYBR Green Realtime PCR Master Mix -Plus-] dntp MgCl 2 Taq DNA Taq PCR SYBR Green I

5 [ ] PCR 20 30mer GC 40 60% 200bp 150bp bp [ ] PCR [ PCR ] ABI PRISM 7700 LightCycler TM LineGene PCR DNA [cdna] 1st strand cdna 1st strand cdna dt PCR 1st strand cdna ReverTra Ace - - (Code:FSK-101) 10 cdna ( ) PCR [ DNA ] PCR DNA 1 10ng DNA

6 ABI PRISM 7700 (1) [PCR ( )]( 0.4 M 50 L) 16 L SYBR Green Realtime PCR Master Mix -Plus- 25 L 1 (10 M) 2 L 2 (10 M) 2 L 5 L 50 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) M (2)PCR 3 ( 1) Tm PCR 1 5 Detector SYBR Quencher None PCR data collection 30 2 ( 2) data collection

7 [PCR ( 1)]( ) (data collection) [PCR ( 2)](2 60 ) (data collection)

8 Roche LightCycler TM (1) [PCR ( )]( 0.6 M 20 L) 5.6 L SYBR Green Realtime PCR Master Mix -Plus- 10 L 1 (10 M) 1.2 L 2 (10 M) 1.2 L 2 L 20 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) M (2)PCR Tm PCR bp 15 data collection 10 ( ) Detector F1 Temperature Transition Rate 20 /sec( ) PCR Temperature Transition Rate 2 /sec 2 data collection

9 [PCR ( )]( ) (data collection) (Melting Curve)

10 BioFlux LineGene (1) [PCR ( )]( 0.4 M 10 L) 2.2 L SYBR Green Realtime PCR Master Mix -Plus- 5 L 1 (10 M) 0.4 L 2 (10 M) 0.4 L 2 L 10 L SYBR Green Realtime PCR Master Mix -Plus- 1/2 ( ) M (2)PCR 3 Tm PCR 1 5 Channel F1 2 data collection [PCR ( )]( ) (data collection) (Melting Curve)

11 (1) ( ) SYBR Green I (2) PCR PCR GC cdna (1) ( ) (2) cdna

12 PCR PCR GC (1) PCR ( ) (2) ( ) PCR

13 Code No. 1st cdna ReverTra Ace - - ( dntps ) 100FSK-101 ReverTra Ace 10,000U 1 50,000U 1 TRT-101 TRT-102 RNase Inhibitor (Native type) 2,500U 1 2,500U 5 SIN-101 SIN-102 RNase Inhibitor (Recombinant type) 2,500U 1 2,500U 5 SIN-201 SIN-202 Realtime PCR Master Mix 1mL 1 QPK-101T 1mL 5 QPK-101 SYBR Green Realtime PCR Master Mix 1mL 1 QPK-201T 1mL 5 QPK BFFQD-33A LineGene ( )

14 TEL FAX order_lifescience@bio.toyobo.co.jp TEL FAX order_lifescience@bio.toyobo.co.jp TEL FAX :00 12:00, 13:00 17:00 techosk@bio.toyobo.co.jp URL

(2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) 1 RT-PCR (10) (12) (13) F. Hoffmann-La Roche Ltd. Roche Molecular

05-8 SYBR Green Realtime PCR Master Mix (Code No. QPK-201, QPK-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3314K (2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux