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1 Journal of Environmental Biotechnology Vol. 13, No. 2, , 2013 総説 ( 一般 ) ベンゼン汚染土壌地下水の好気的及び嫌気的バイオレメディエーション技術の開発 Development of the Aerobic and Anaerobic Bioremediation Techniques for Benzene-contaminated Soil and Groundwater Yoh Takahata TEL: FAX: yoh.takahata@sakura.taisei.co.jp Taisei Technology Center, Soil and Rock Engineering Research Section, Civil Engineering Research Institute, Technology Center, TAISEI CORPORATION, 344-1, Nase-cho, Totsuka-ku, Yokohama , Japan キーワードDN11 Key words: bioremediation, bioaugmentation, monitored natural attenuation, biosparging, strain DN はじめに 1 10) 1.8 g/l 熊本市内のガソリン汚染サイトにおけるベンゼンの挙動と生物学的自然減衰 13)

2 112 1 BTX 3 BTX m 1 10 BTX Monitored Natural Attenuation 19) MNA BTX 17) 2 3 m, p- 6) BTX BTX MNA MNA 2 3 NH mg/l PO mg/l mg/l 3 NH mg/l PO mg/l NO mg/l NH mg/l PO mg/l SO mg/l 4.0 mg/l

3 113 Azoarcus 7) 3. 好気的な原位置バイオレメディエーション技術の開発 BTX BTX 2 MNA 4 8 7) 1 2 BTX MNA BTX PCR-DGGE 7) ) 2 11,12) 5 1BTX ph 5<pH<9 <5 C >20 C ph * MAN * C 19 C cells/ml cells/ml 186 mg/l 25 mg/l 0.6 mg/l 7.6 mg/l 1.0 mg/l 5.0 mg/l 0.6 mg/l 15.9 mg/l

4 No m 2 15) /10 2 No.7 18) 4. 嫌気的なバイオレメディエーション技術の開発 5 1) 5) 6 10 m 11) 10 kg 2 No. 1 65,000 m , 3,000 m ,000 m ,000 m ,000 m ,000 m ,000 m 3 14

5 NEDO 2 RNA-SIP 3) DN DN11 16S rdna Azoarcus Dechlorompnas RCB 2) 2 DN11 m- DN11 DN11 14) DN11 DN11 14) DN11 4,16) 10 7 No. 6 1 DN11 8 A 9 BSM 4) DN11 20 C

6 116 文献 10 DN11 DN11 16) DN11 DN11 9) DN11 DN11 5. おわりに謝辞 1) Burland, S.I. and E.A. Edwards Anaerobic benzene biodegradation linked to nitrate reduction. Appl. Environ. Microbiol. 65: ) Chakraborty, R., S.M. O Connor, E. Chan, and J.D. Coates Anaerobic degradation of benzene, toluene, ethylbenzene, and xylene compounds by Dechloromonas strain RCB. Appl. Environ. Microbiol. 71: ) Kasai, Y., Y. Takahata, M. Manefield, and K. Watanabe RNA-based stable isotope probing and isolation of anaerobic benzene-degrading bacteria from gasoline-contaminated groundwater, Appl. Environ. Microbiol. 72: ) Kasai, Y., Y. Kodama, Y. Takahata, T. Hoaki, and K. Watanabe Degradative capacities and bioaugmentation potential of an anaerobic benzene-degrading bacterium strain DN11, Environ. Sci. Technol. 41: ) Lovley, D.R., J.D. Coates, J.C. Woodward, and E.J.P. Phillips Benzene oxidation coupled to sulfate reduction. Appl. Environ. Microbiol. 61: ) Takahata,Y., Y. Kasai, and K. Watanabe Assessment of chemical and microbiological signatures during natural attenuation of gasoline-contaminated groundwater, European symposium on environmental biotechnology, ESEB ) Takahata, Y., Y. Kasai, T. Hoaki, and K. Watanabe Rapid intrinsic biodegradation of benzene, toluene, and xylenes at the boundary of a gasoline-contaminated plume under natural attenuation. Appl. Microbiol. Biotechnol. 73: ) United States Environmental Protection Agency How to Evaluate Alternative Cleanup Technologies for Underground Storage Tank Sites, Chpter VIII Biosparging, EPA 510-B ) 2012 DN11 67 VII-150: ) 2009 Vol. 57, No. 7, Ser. No. 618: ) 2009 F Vol. 65, No. 4: ) 2010 F Vol. 66, No. 5: ) 2010 Vol. 52, No. 1: ) 2007 DN11 No. 40, ) 2012 Vol. 53, No. 8: ) : ) 2008MNA BTX. Vol. 50, No. 3: ) toyosusinsijyo-kouji.jp/progress/ 19) 2012 pp

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