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1 Effects of the testosterone on both male and female fowls Shohyoe Tsuda

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10 Résumé This study was carried out to test the biological actions of androgen on both male and female fowls. As androgen "Amolisin" was used; the "Amolisin" is oil solution of testosterone acetate. Fourteen fowls ( 8 females and 6 males), were used. Each fowl was given 50 mg of "Amolisin" during a period of 20 days by daily subcutaneous injections. The results of the investigations may be summarized as follows : Combs and wattles became enlarged, above all female combs extremely larger. Remarkable changes of the difference in their living weights could not be found between treated fowls and controls. After 5 or 6 days from the beginning of this treatment, the arrest of laying occurred in female fowls. Then after two weeks' injections they began to crow and behaved as if they were active males. They also imitated the male gestures of copulation to the control fowls. In anatomical observations, "Gefassreicher Korper (nach Liebe)" could not be found out in female fowls. Injected cocks' "Gefassreicher Korper" became more intensive in their reddish color. Testes, ovaries and oviducts decreased exceedingly in weight and size. Under histological observations, the hormone accomplished its effect upon the comb primarily by stimulating the cells of the intermediate layer of loose connective tissue whose fibers were widely spread apart by a matrix of mucoid; especially the comb of injected hen had an intermediate layer much highly developed. In the "Gefassreichem Korper", loose connective tissue was found to have entered into marrow core tissue, and lymphatic sinuses were filled with lymph fluid. According as the shrinkage of the testis, the seminiferous tubules were reduced markedly in size and spermatogenesis was inhibited. Under the

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