食糧 その科学と技術 No.43( )
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1 53 inclusion body: CA 2001 CA
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6 58 CA : 17 CA 9 CA CA CTAB C16 C16, C18 26 CA SDS CA CA 10 CA 3 CitSyn 49,000/ B CAB 30,000 Zn 15,000 4
7 59 S-S CitSyn 6 M 1 30 CA CD Tween40 Tween CA CA 1 5
8 60 CA CA; CA 7 CA; CA 2 B CAB 16 CitSyn CTAB SB mm DTT 6 M 16 S-S CAB CTAB SB3-14 DL- CA S-S
9 61 CA 6 1 Lowry 25 30mg/ml 6 M A S-S 40mM DTT 2 4 Tween 40, Tween 60
10 62 8 l 25 l of 8M : 6M 0.3 l of 4M DTT 40 mm (, 1 8 l 560 l of ( 0.1% 2mM DL-cystine, l of 3 % CA ( 0.6%, 1 (15,000rpm 5 min CTAB, SB CTAB SB3-14 S-S DL- S-S - :GSSG GSH 10 DL- GSH/GSSG DL- 3 3 CA CA 0.6 CA
11 63 CA CA CA CA CA CA 1 15,000rpm 5 CA 12 CA 1 SDS-PAGE
12 64 2 circular dichroism:cd BioRad BioBeads 1/ CA 5, LDL human C-type lectin like oxidized low density lipoprotein receptor hlox-1 3 C 7 hlox-1 CA 90 8 CD CA 9 hlox-1 LDL LDL LDL LDL C 12,13
13 65 C 273 C C C 183 C C 142 C N C-type lectin-like oxidized LDL receptor : hlox-1 3 S-S 3 2 : 40,000 : C-type lectin-like domain (CTLD) : 細胞外領域
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16 68 Kit 1 Marston,F.A.O. 1986, Biochem.J., 240, Wetlaufer,D.B.and Xie,T 1995, Prot.Sci., 4, D.Rozema, et al., J. Am. Chem. Soc., 117, M.Yoshimoto. et al., J.Chromatogr.B, 743, Y.Akiyoshi, et al., Bioconjugate Chem., 10, R.Kuboi. et al., J.Chromatogr.B, 743, BioView, 41, D.Rozema, & S.H.Gellman., Biochemistry, 35, T.Takaha, et al., J.Biol.Chem., 271, S.Machida, et al., FEBS lett., 486, Q.Xie, et al., Protein Expression and Purification, 32, S.Machida, et al., US patent
食糧 その科学と技術 No.43( )
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