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1

2 COLAK Mehmed

3

4

5

6

7

8

9 GH IGF-1

10

11

12

13 GH GH

14

15

16

17

18

19

20

21 Kawashima et al., J Reprod Dev, 52 (2006)

22

23

24

25

26 1 Kawashima et al., J Dairy Sci, 90 (2007)

27 Kawashima et al., J Dairy Sci, 90 (2007)

28 Kawashima et al., J Dairy Sci, 90 (2007)

29

30

31

32

33 P<0.001 vs Kawashima et al., Reproduction, 133 (2007)

34 P<0.05 P< P<0.01 P< Kawashima et al., Reproduction, 133 (2007)

35

36

37 P< vs P< vs Kawashima et al., J Reprod Dev, 53 (2007)

38 P< vs. Kawashima et al., J Reprod Dev, 53 (2007)

39

40 IGF (Insulin-like growth factor), IGF-I IGF-II (Type I II) (1-6)

41

42

43

44 E2

45 Kawashima et al., Reproduction, 133 (2007)

46 P< Kawashima et al., Reproduction, 133 (2007)

47 0.8 n=5 0.8 n= E Kawashima et al., Reproduction, 133 (2007)

48

49

50 Exp.1. ; (1) 1 1 cm 2 mm Aloka SSD cm 11

51 Exp.2. LH ; (1) GnRH 1 cm Pre GnRH GnRH 0.5 h 1 h 2 h 6 h 12 h 24 h 30 h 48 h Acosta et al., Reproduction, 125 (2003)

52 Exp.2. LH ; (1) 1 cm -30 h -24 h -18 h -6 h 0 h LH 6 h 12 h 24 h Acosta et al., Reproduction, 125 (2003)

53 Exp.2. LH ; (1) 42

54 GH

55 LH (E2) FSH LH FSH LH

56 LH LH P4 P4 P4 P4

57

58 FSH LH E2 P4 GH IGF-1?? 5

59 LH >8 mm 1 P4 2 PGF2α 2 42 h 1 LH P4 P4 39

60 PGF2α 42h 3 6

61 VS. (P4 E2) LH GH IGF

62 12 mean SEM F1 F2 F3 F4 12 mean SEM (mm) (mm) Day 1 F1 F2, F3, F4 (P<0.05) Day 1 F1, F2 F3, F4 (P<0.05) 41

63 1 P4 (ng/ml) P4 mean SEM *: P<0.05 **: P<0.01 ***: P<0.001 Between two groups * * * * ** *** E2 (pg/ml) E2 mean SEM *: P<0.05 Between two groups * * * * FSH (ng/ml) FSH mean SEM 2 42

64 LH 1 F1 F1 F2 F3 F2 GH 51

65 mrna expression (x10 4 ) mean SEM a,b: P<0.05 a LH x b y c z mrna expresion (x10 4 ) mean SEM a,b: P<0.05 x,y: P<0.05 a x 0 GH b y b xy

66 1 IGF mean SEM a,b: P<0.05 a 1.5 mean SEM IGF-1 (ng/ml) b b b ab ab ab ab Insulin (ng/ml) Day 2 GH???? 47

67 GH LH IGF-1 2 F1 F2 F1 F2 E2 LH P4 P4 LH 2 P4 E2 LH GH GH IGF

68 : : EIA : P4 GH IGF-1

69 P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) ab a ab ab ab ab ab b a cd bc ab bcd cd cd d a ab bc ab cd de e de P4 (ng/ml) IGF-1 (ng/ml) Insulin (ng/ml) GH (ng/ml) a b b b b b ab 4 ab 3 abc 2 1 d cd b b a abc bcd Kawashima et al., J Reprod Dev, 53 (2007)

70 E2 LH GH P4?? IGF-1

71 GH LH E2 P4?? LH GH IGF-1 5

72

73 Mehmet ÇOLAK

74

75 Growth hormone GH Insulin? Steroids IGF-1 Insulin-like growth factor-1

76 Control (n=3) P4 (n=4) 2 CIDR BIOPSY SLAUGHTERHOUSE EB inj IM (1 mg) BIOPSY SLAUGHTERHOUSE E2 (n=3) BIOPSY SLAUGHTERHOUSE Serial Blood Collection 15 min, 16 h Daily Blood Collection

77

78 Analysis of Plasma E2, P4, GH, IGF-I and Insulin Hormone analyses were performed by EIA Analysis of GHR, IGFR-I and IR genes Quantitative PCR method using BioRad

79 P4 (ng/ml) *** *** *** *** *** *** *** Mean S.E.M. ***:P< E2 (pg/ml) *** *** DAY

80 Control GH (ng/ml) P4 group E2 group Time (h)

81 (ng/ml/min) a b c Mean S.E.M. a, b, c: P<0.05 (ng/ml) a b a PULSAR program 0 (16 h) b a a Control E2 P4

82 140 Control P4 E2 * Mean S.E.M. *:P< IGF-I (ng/ml) Before After Before After Before After

83 Total GH-R P=0.055 mrna ( 10-7 ) Insulin-R mrna IGF-R1 mrna ( 10-6 ) b a a Control E2 P4 b a a Mean S.E.M. a, b: P<0.05

84 Growth hormone GH E2 IGF-I GHR IGFR-I IR

85 Estradiol+Progesterone Eimeria bovis * * * * * * * * Percentage Feed Intake Day Heath et al, Am J Vet Res (1997)

86 Estrogen+Proegesterone, but not GH *a a *a *a *a Weight Gain (kg) Con EP GH PF C EP\C GH\C * a Heath et al, Am J Vet Res (1997)

87

88 350 Duration (min) * * * *,* * C: control E: endotoxin S: Synovex S (P/E) C/E Saliva S/E Saliva C/E Pant S/E Pant C/E Cough S/E Cough McMahon et al, J. Endocrinol., (1998)

89

90

91

92

93 Growth hormone GH E2 IGF-I GHR

94 Growth hormone GH E2 P4 GHR IGF-I GHIGF-I E2/P4

95 E/P

96 IGF IGF-1 IGF-1

97

98

99

100 Thank you for your attention!!

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