微生物遺伝資源利用マニュアル(13)
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1 ISSN MAFF Microorganism Genetic Resources Manual No.13 Colletotrichum acutatum Plurivorous plant anthracnose fungus, Colletotrichum acutatum Colletotrichum acutatum Simmonds ex Simmonds MAFF MAFF Colletotorichum acutatum 1965 CMI Descriptions of pathogenic fungi and bacteriadyko and Mordue, 1979 Sutton1980 Colletotrichum Sutton1980 Colletotrichum gloeosporioides (Penzig) Penzig & Saccardo C. gloeosporioides Sato et al., van der Aa et al. C. gloeosporioides Sato et al., Yoshida and Tsukiboshi, 2002 C. gloeosporioides DNA ITS cm 30% 23 PDA 15ml PDA mm PDA PDA 1
2 PDA PDA 1525, 25FL20SBLB PDA 10 ( 10P5P 10P6P/ml), , 2025, 7,8 PDA PDA PDA 12 FL20SBLB 12 1, 2, 5 3MAFF (6) (22) 2.546µmPCA: (6)8.510(15) 4.56(9.8)µm 6 2
3 Sato et al., 1996, 1997; Yoshida and Tsukiboshi, 2002 Colletotrichum gloeosporioides PDA C. acutatum C. gloeosporioides 5 C. acutatum C. gloeosporioides Simmonds1965"larger spored form" MAFF C. gloeosporioides C. acutatumc. gloeosporioides C. acutatum C. gloeosporioides C. acutatum C. gloeosporioides PCA C. acutatum 6C. gloeosporioides Sutton, Gunnell and Gubler, ,250ppm 625ppm 400 PDA 9cm 40 1ml PDA 9ml PDA 6mm 25 5 PDA (%)C. acutatum 48 C. gloeosporioides , C. gloeosporioides 8 G C. acutatum
4 C. acutatum C. gloeosporioides Colletotrichum acutatum C. gloeosporioides C. acutatum C. gloeosporioides C. acutatum C. acutatumc. gloeosporioides C. gloeosporioidesg. cingulata PDA cm C. acutatum cm C. gloeosporioides PCA * C. acutatum C. gloeosporioides ** PDA C. acutatum C. gloeosporioides C. gloeosporioides PDA C. acutatum C. gloeosporioides *PCA 20gl, 10 1l 15g ** Sreenivasaprasad et al rdna ITS1 Sreenivasaprasad et al.,
5 DNA Sambrook et al. (1989) Rodriguez and Yoder (1991) PDA, 25 4, 0.1g 1.5ml 0.5 ml DNA (150 mm EDTA, 50 mm Tris-Cl (ph 8), 1% (w/v), 500 g/ml pronase E) (TGK) 65 15, (15,000 rpm, 10, ), 0.7 PEG/NaCl (20% polyethylene glycol 8000, 2.5 M sodium chloride), 515 (15,000 rpm, 10, ), 0.5 ml TE (10 mm Tris-Cl (ph 7.5), 1mM EDTA) 2.5 M, 20 (15,000 rpm, 10, ), 0.6, 30 (15,000 rpm, 10, ), 0.25 ml TE 2.5 M, 20 (15,000 rpm, 10, ), 0.1 M,, 2, 30 (15,000 rpm, 10, ) DNA 70,, 0.1 ml TE, DNA PCR 2, ITS1 (White et al., 1990) NL4 (Guadet et al., 1989) PCR, RNA (ITS), 5.8S RNA, ITS2, 28S 1, 2 RNA (rdna) PCR PCR (50 µl) 50 mm KCl, 20 mm Tris-Cl (ph 8.4), 2 mm MgCl, 200 µm dntp, 0.4 µm, 1 µl DNA ( 10 ng), 1.25 Taq () PCR thermal cycler MP (), 9530, 5530, DNA 721 1, 35 RNA rdna PCR, CEQ2000 (Beckman Coulter, Inc.) Dye Terminator Cycle Sequencing Kit (Beckman Coulter, Inc.), ITS1, ITS2 (White et al., 1990) 2, C. acutatum rdna ITS 181bp DNA (DDBJ) C. acutatum Z32923Moriwaki et al., 2002 Aa, H.A. van der, M.E. Noordeloos and J. de Gruyter (1990): Species concepts in some larger genera of the Coelomycetes. Stud. Mycol., 32: Dyko, B.J. and J.E.M. Mordue (1979): Colletotrichum acutatum. CMI Descriptions of pathogenic fungi and bacteria. No Commonwealth Mycological Institute, Kew. Guadet, J., J. Julien, J. F. Lafay, and Y. Brygoo (1989): Phylogeny of some Fusarium species, as determined by large-subunit rrna sequence comparison. Mol. Biol. Evol. 6:
6 Gunnell, P.S. and W.D. Gubler (1992): Taxonomy and Morphology of Colletotrichum species pathogenic to strawberry. Mycologia, 84: Colletotrichum acutatum Simmonds, Moriwaki, J., T. Tsukiboshi and T. Sato (2002): Grouping of Colletotrichum species in Japan based on rdna sequences. J. Gen. Plant Pathol. 68 : Rodriguez, R.J. and O.C. Yoder (1991): Isolation of Glomerella musae [teleomorph of Colletotrichum musae (Berk. & Curt.) Arx] and segregation analysis of ascospore progeny. Exp. Mycology 15: Sambrook, J., E.F. Fritsch and T. Maniatis (1989): Molecular cloning: A laboratory manual, 2nd ed., Cold Spring Harbor Laboratory Press, New York. 1996, Colletotorichum acutatum, Sato, T., S. Ueda, A. Iijima, and N. Tezuka1996Re-identification of pathtogen of anemone and prune anthracnose. Ann. Phytopath. Soc. Jpn., 62: Sato T., S. Uematsu, H. Mizoguchi, T. Kiku and T. Miura1997Anthracnose of prairie gentian and loquat caused by Colletotrichum acutatum. Ann. Phytopath. Soc. Jpn., 63: Colletotrichum acutatum C. acutatum Glomerella cingulata Simmonds, J.H. (1965): A study of the species of Colletotrichum causing ripe fruit rots in Queensland. Qd. Jl. Agr. Anim. Sci., 22: Sreenivasaprasad, S., A.E. Brown and P.R. Mills (1992): DNA sequence variation and interrelationships among Colletotrichum species causing strawberry anthracnose. Physiol. Mol. Pl. Path., 41: Sreenivasaprasad, S., P.R. Mills and A.E. Brown (1994): Nucleotide sequence of the rdna spacer 1 enables identification of isolates of Colletotrichum as C. acutatum. Mycol. Res., 98:
7 Sutton, B.C. (1980): Colletotrichum. In The Coelomycetes. Fungi Imperfecti with Pycnidia, Acervuli and Stromata: Commonwealth Mycological Institute, Kew, pp White, T.J., T. Bruns, S. Lee, and J. Taylor (1990): Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR protocols, a guide to methods and applications (Innis, M.A, D.H. Gelfand, J.J. Sninsky and T.J. White eds.). Academic Press, New York, pp Colletotorichum acutatum Simmonds ex Simmonds 65: Yoshida, S. and T. Tsukiboshi (2002): Shoot blight and leaf spot of blueberry anthracnose caused by Colletotrichum acutatum. J. Gen. Plant Pathol. 68: HThttp:// MAFF No. MAFF 1 HT237130TH 2(10) 2 HT237168TH T. Kobayashi-22(9) 3 HT237215TH 60KF543 LC 4 HT237216TH 93KLC-2 LC 5 HT237217TH K1-1 LC 6 HT237218TH 3KEC LC 7 HT237240TH Kishi-5(4) 8 HT237758TH T. Kobayashi-38(3) 9 HT237894TH T. Kobayashi-40(2) 10 HT237922TH -1(6)(Pla-75) 11 HT305596TH 1 LC 12 HT306172TH 1 LC 7
8 13 HT306247TH E-2 LC 14 HT306248TH E-6 C 15 HT306249TH 1 C 16 HT306250TH 1 C 17 HT306251TH To9101 LC 18 HT306252TH To9102 LC 19 HT306253TH To9106 LC 20 HT306254TH To9107 LC 21 HT306282TH Na LC 22 HT306283TH LC 23 HT306404TH Ca 1 LC 24 HT306405TH Ca 2 LC 25 HT306406TH Cg 1-2 LC 26 HT306407TH Cg 5-1 LC 27 HT306408TH Tss 7 C 28 HT306409TH A-12 C 29 HT306410TH T HT306430TH FPeCG 9301 LC 31 HT306487TH AU2 C 32 HT306488TH AC1 C 33 HT306489TH PSS 3 C 34 HT306490TH KL1-4 C 35 HT306502TH To 9103 C 36 HT306503TH GCP 29 LC 37 HT306504TH GCP 11 LC 38 HT306505TH GCP 26 LC 39 HT306506TH CB 5 LC 40 HT306507TH AU-1 LC 41 HT306508TH FLS 151 LC 42 HT306509TH TSS 1 LC 43 HT306520TH MCR-1 C 8
9 44 HT306521TH MCR-2 C 45 HT306522TH MPK-1 C 46 HT306523TH MPK-2 C 47 HT306524TH MPK-3 C 48 HT306525TH MPK-4 C 49 HT306526TH MMU-1 C 50 HT306527TH MMU-2 C 51 HT306528TH MCA-1 C 52 HT306529TH MCA-2 C 53 HT306542TH HF3 LC 54 HT306543TH C11 LC 55 HT306544TH C24 LC 56 HT306545TH SL5-1C1 LC 57 HT306546TH EA1 LC 58 HT306547TH EA2 LC 59 HT306548TH EA3 LC 60 HT306549TH GCA6 LC 61 HT306550TH CC1 C 62 HT306551TH Mo9301 () C 63 HT731068TH CA-5 C 64 HT731069TH CA-8 C 65 HT840072TH S9303 LC 66 HT840073TH S9307 LC 9
10 Colletotrichum acutatum Colletotrichum gloeosporioides 9. C. acutatum PDA PSS3=MAFF CB5=MAFF C. acutatum PDA AU2=MAFF CB5=MAFF C. acutatum 20m C. acutatum ( EA1=MAFF306546)C. gloeosporioides "Larger spored form" 10m C. acutatum C. gloeosporioides, FpeCG9301=MAFF306430, TSS1MAFF C. gloeosporioides AC. acutatum GC. gloeosporioides PCA257 C. acutatum AU2MAFF m C. gloeosporioides m C. acutatum (A) C. gloeosporioides (G) 2511 PDA PDA GC. gloeosporioides PDA 10
11 11
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714 植 物 防 疫 第 63 巻 第 11 号 2009 年 岡山県で発生した Rhizobium radiobacter Ti による ブドウ根頭がんしゅ病 岡山県農業総合センター農業試験場 は じ め かわ ぐち あきら 川 口 章 12 月のサンプルから AT06 1 AT06 8 株 を
714 植 物 防 疫 第 63 巻 第 11 号 2009 年 岡山県で発生した Rhizobium radiobacter Ti による ブドウ根頭がんしゅ病 岡山県農業総合センター農業試験場 は じ め かわ ぐち あきら 川 口 章 12 月のサンプルから AT06 1 AT06 8 株 を選抜し に 以下の試験に供試した ブドウ根頭がんしゅ病は土壌伝染性の細菌病であり II 保菌苗木の流通によって病原細菌が広範囲に伝搬され
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mRNA-Seq_SamplePrep.book
mrna Sequencing Sample Preparation Guide Topics 3 Introduction 4 RNA Input Recommendations 6 mrna-seq Sample Preparation Kit Contents 8 User-Supplied Consumables and Equipment 9 Purify the mrna 12 Fragment
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