ソリッドコアUHPLCカラムとUHPLCシステム、質量分析計を利用した違法薬物の高速スクリーニング分析
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- きゅういち わしあし
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1 UHPLCUHPLC Application Note CC2999 Vanquish Accucoreopiates benzodiazepines amphetamines Thermo Scientific Accucore Vanquish C18 UHPLC1.5 m Vanquish UHPLC 47 UHPLC Vanquish UHPLC Accucore Vanquish UHPLC Accucore Vanquish UHPLC 1.5 m Accucore Vanquish 1.5 m Accucore Vanquish UHPLCVanquish UHPLC Vanquish UHPLC 15 MPa 15 bar
2 2 Accucore Vanquish C m UHPLC mm LC-MS 18 M Thermo Scientific Smart2Pure Fisher Chemical LC-MS Fisher Chemical analytical Fisher Chemical Thermo Scientific Virtuoso 9 mm 2 ml 618-VT mg1 ml/ 1:1, v/v 1 mg/ml / 9:1, v/v1 ng/ml Thermo Scientific Virtuoso ID Vanquish UHPLC Vanquish Vanquish Vanquish Thermo Scientific TSQ Vantage Virtuoso ID VH-P1-A VH-A1-A VH-C1-A 618-VT-1 : Thermo Scientific Xcalibur 2.1 MS control Thermo Scientific Dionex Chromeleon 7.2 LC/MS UHPLC : A: B: : : : : Accucore Vanquish C m mm 1 mm.1% 5 L/min Forced air mode 5 2 L 1 1: % B TSQ Vantage SRM selected-reaction monitoring2 347SRM
3 2 : TSQ Vantage 3 HESI Positive V 45 5 Arb 75 Arb 2 38 mtorr 1. s.5 Q1 FWHM.7 Q3 FWHM.7 3: 47 SRM RF- V V min Ecgonine methyl ester Normorphine Dihydromorphine Anhydroecgonine methyl ester (AEME) Oxymorphone Norcodeine Morphine Noroxycodone Hydromorphone Norhydrocodone Amphetamine MDA Methamphetamine O-desmethyl(-cis-) tramadol Dihydrocodeine N,O-didesmethyl tramadol MDEA Benzoylecgonine Oxycodone acetylmorphine Codeine Norfentanyl Hydrocodone N-desmethyl tapentadol N-desmethyl tramadol cis-tramadol Naltrexone Tapentadol Normeperidine Meperidine Nortilidine Naloxone acetylcodeine
4 4 3: 47 SRM RF- V V min Norbuprenorphine PCP (phencyclidine) ,5-dimethyl-3,3-diphenylpyrrolodone (EDDP) Clonazepam Tilidine Oxazepam Methadone Lorazepam Alprazolam Flurazepam Fentanyl Nordiazepam nor- -9 -carboxy-thc Buprenorphine Vanquish UHPLCAccucore Vanquish UHPLC 47 6 SRM1 5 L/min 89 MPa 89 bar MPa 1233 baraccucore Vanquish UHPLC 4,5, 4,, 3,5, 3,, Abundance 2,5, 2,, 1,5, 1,, 5, -5, : 47 SRM
5 24SRM 2m/z 28 34m/z Relative Abundance m/z m/z : N-desmethyltapentadol MDEA m/z 28 Relative Abundance m/z m/z : codeine hydrocodone m/z 3
6 Relative Abundance m/z m/z Application Note CC2999 4: naltrexone 6-acetylcodeine m/z 342 naltrexone Accucore Vanquish C m UHPLC Vanquish UHPLC 47 6 Vanquish UHPLC 1 McHale, K., Thermo Scientific, Quantitative LC-MS Screening for Illicit Drugs Using Ultrahigh Resolution Mass Analysis and Accurate Mass Confirmation, Thermo Scientific Application Note 21 AN63166 b_e8 /1 S. C214 Thermo Fisher Scientific Inc. TEL FAX E151 Analyze.jp@thermofisher.com
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