Ann. Phytopath. Soc. Japan 50: 149-157 (1984) Toshiki SHIOMI* and Miyoji SUGIURA**: Grouping of Mycoplasma-like Organisms Transmitted by the Leafhopper Vector, Macrosteles orientalis Virvaste, Based on Host Range Abstract Mycoplasmalike organisms (MLOs) transmitted by the leafhopper vector, Macrosteles orientalis Virbaste, were compared in host range and symptoms in test plants. Twelve MLO diseases from Japan, including Japanese honewort witches' broom, lettuce yellows, sickle hare's ear yellows, garland chrysanthemum witches' broom, water dropwort yellows, strawberry witches' broom, onion yellows, and carrot yellows were considered to be caused by the agents closely related each other. From the result of inoculation test with M. orientalis to the test plants including 20 species in 11 families, these MLO isolates were classified into three strains. Strain I infected Lactuca sativa, Callistephus chinensis, Chrysanthemum coronarium, Daucus carota, Apium graveolens, Spinacia oleracea, Allium cepa and others. Strain II infected Brassica rapa, Pisum sativum, Solanum melongena, Nicotiana glutinosa, and others, including the host plants of strain I. Strain III infected Lycopersion esculentum in addition to the host plants of strain II. Strain II and III did not infect Geranium nepalense which was a host plant of strain I. (Received September 8, 1983) Key Words: grouping, MLO strains, host range, Macrosteles orientalis.
Table 1. List of MLO isolates used and its transmission rate with the leafhopper vector, Macrosteles orientalis a) Cryptotaenia japonica seedlings were used as test plants. b) Number of infected plants/number of inoculated plants.
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Explanation of plates Symptoms in test plants. Plate I Fig. 1. Stunting in Dianthus barbatus. Fig. 2. Yellows and witches' broom in Spinacia oleracea. Fig. 3. Phyllody in Callistephus chinensis. Fig. 4. Stunting and witches' broom in Chrysanthemum coronarium. Fig. 5. Stunting and yellows in Lactuca sativa. Fig. 6. Stunting and yellows in Cucurbita moschata. Fig. 7. Stunting in Geranium nepalense. Fig. 8. Stunting and witches' broom in Cardamine flexuosa (D), and healthy one (H) Plate II Fig. 9. Stunting and witches' broom in Astragalus sinicus. Fig. 10. Stunting and yellows in Pisum sativum. Fig. 11. Stunting and yellows in Allium fistulosum (D), and healthy one (H). Fig. 12. Witches' broom and phyllody in Nicotiana glutinosa. Fig. 13. Deformation and leaf curl in Solanum melongena. Fig. 14. Sunting and yellows in Apium graveolens. Fig. 15. Yellows and witches' broom in Cryptotaenia japonica. Fig. 16. Witches' broom in Daucus carota. Plate III Fig. 17. Witches' broom in Catharanthus roseus. Fig. 18. Stunting and witches' broom in Brassica raga. Fig. 19. Stunting and yellows in Allium cepa (D), and healthy one (H). Fig. 20. Stunting and yellows in Lycopersicon esculentum.
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Plate II
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