Journal of Environmental Biotechnology Vol. 15, No. 2, 71 75, 2016 総説 ( 特集 ) セレン代謝微生物を利用したセレン含有排水処理技術の開発 Development of Selenium-Containing Wastewater Treatment Technologies Using Microbial Selenium Transformation * Michihiko Ike*, Satoshi Soda and Masashi Kuroda 565 0871 2 1 * TEL: 06 6879 7672 FAX: 06 6879 7675 * E-mail: ike@see.eng.osaka-u.ac.jp Graduate School of Engineering, Osaka University, 2 1 Yamadaoka, Suita, Osaka 565 0871, Japan キーワード Pseudomonas stutzeri NT-I Key words: Selenium-containing wastewater, treatment, microbial selenium metabolism, Pseudomonas stutzeri NT-I 2016 2 8 2016 2 15 1. はじめに 0.1 mg/l 6 2 SeO 4 2 : Se(VI) SeO 3 2 : Se(IV) 20 Se(VI) Se(VI) Se(IV) Lenz & Lens, 2009 7) Soda et al., 2011 9) Haygarth, 1994 2) 1 Se(VI) Se(IV) Se(0) Soda et al., 2012 10) Kagami et al., 2013 3) 2. 多才なセレン代謝細菌 Pseudomonas stutzeri NT-I Se(VI) P. stutzeri NT-I Kuroda et al., 2011 5) NT-I Se(VI) Se(0) NT-I
72 1 2 Pseudomonas stutzeri Se(VI) serabdc serxsr TSB Trypticase Soy Broth 80 mg-se/l Se(VI) 18 Se(IV) Se(0) Se(VI)/Se(IV) Se(0) 10 200 nm NT-I Se(0) NT-I Se(0) Kagami et al., 2013 3) DMDSe DMDSe NT-I Se(VI) Se(IV) NT-I Se(VI) Se(VI) Thauera selenatis serabdc Krafft et al., 2000 4) Kuroda et al., 2013 6) 2 NT-I Se(VI) serabdc Se(VI) serabc redox enzyme maturation proteins REMPs Turner et al., 2004 11) serd T. selenatis serabdc 3 ORF 2 Se(VI) T. selenatis NT-I Se(VI) serxsr Se(VI) Se(IV) Se(IV) NT-I NT-I Se(VI) Se(IV) NT-I Se(IV) DMDSe NT-I DMDSe DMDS
73 3 A B Se(IV) cysi O-acetyl-l-serine l-cysteine cysk NT-I NT-I 3. P. stutzeri NT-I を利用したセレン含有排水処理技術の開発 NT-I 3.1 セレン製錬排水 3 NT-I Soda et al., 2012 10) NT-I ph 1 6 7 Soda et al., 2011 9) NT-I Se(VI) ph 7.0 9.0 NaCl 0.05 20 g/l Se(VI) ph 6.0 9.0 NaCl 0.05 50 g/l ph 6 7 2 4 P. stutzeri NT-I 255 L NT-I 1 7 4 55 60 mg-se/ L Se(IV) 5 0.1 1 mg-se/l 30 mg-se/l Se(VI) 5 Se(0) TSB Kagami et al., 2013 3) 3.2 汚泥焼却灰洗浄排水 0.3 mg/l 0.01 mg/l
74 5 2014 12) 3.8 mg-se/l 10.0 g 100 ml 53 0.20 mg-se/l Se(IV) 0.2 M 74 0.28 mg-se/l Se(IV) 54 Se(VI) 2014 12) 9.7 g 0.06 mg-se/l ph 6.7 10.8 ph 8.5 NT-I 6 48 Se(VI) NT-I Soda et al., 2012 10) Se(0) CdSe Ayano et al., 2014 1) 4. おわりに NT-I NT-I 5 6 P. stutzeri NT-I A B 25 M
75 ph NT-I NT-I Nakatani et al., 2015 8) NT-I 謝 22 24 B 22310047 19 21 文 辞 献 1) Ayano, H., M. Miyake, K. Terasawa, M. Kuroda, S. Soda, T. Sakaguchi, and M. Ike. 2014. Isolation of a selenite-reducing and cadmium-resistant bacterium Pseudomonas sp. strain RB for microbial synthesis of CdSe nanoparticles. J. Biosci. Bioeng. 117: 576 581. 2) Haygarth, P.M. 1994. Global importance and global cycling of selenium. pp. 1 7. In W.T. Frankenberger Jr. and S. Benson, editors. Selenium in environment. New York: Marcel Dekker. 3) Kagami, T., T. Narita, M. Kuroda, E. Notaguchi, M. Yamashita, K. Sei, S. Soda, and M. Ike. 2013. Effective selenium volatilization under aerobic conditions and recovery from the aqueous phase by Pseudomonas stutzeri NT-I. Water Res. 47: 1361 1368. 4) Krafft, T., A. Bowen, F. Theis, and J. M. Macy. 2000. Cloning and sequencing of the genes encoding the periplasmiccytochrome B-containing selenate reductase of Thauera selenatis. DNA Seq. 10: 365 377. 5) Kuroda, M., E. Notaguchi, A. Sato, M. Yoshioka, A. Hasegawa, T. Kagami, T. Narita, M. Yamashita, K. Sei, S. Soda, and M. Ike. 2011. Characterization of Pseudomonas stutzeri NT-I capable of removing soluble selenium from the aqueous phase under aerobic conditions. J. Biosci. Bioeng. 112: 259 264. 6) Kuroda, M., E. Miwa, K. Sei, M. Yamashita, and M. Ike. 2013. Selenate-reduction mechanism in Pseudomonas stutzeri NT-I, a promising biocatalyst for selenium removal and selenium recovery from contaminated water, Abstracts of IWA Metals in Water-Health Protection and Sustainable Through Technical Innovation 2013. November 6 9, 2013, Shanghai, China. The International Water Association. 7) Lenz, M. and P.N.L. Lens. 2009. The essential toxin: the changing perception of selenium in environmental sciences. Sci. Total. Environ. 407: 3620 3633. 8) Nakatani, Y., M. Kuroda, S. Soda, and M. Ike. 2015. Treatment of selenite-containing wastewater with high salinity by activated sludge and analysis of its microbial communities. Abstracts of International Anammox Symposium, pp. 165 166. 9) Soda, S., M. Kashiwa, T. Kagami, M. Kuroda, M. Yamashita, and M. Ike. 2011. Laboratory-scale bioreactors for soluble selenium removal from selenium refinery wastewater using anaerobic sludge. Desalination 279: 433 438. 10) Soda, S., H. Takahashi, T. Kagami, M. Miyake, E. Notaguchi, K. Sei, N. Iwasaki, and M. Ike. 2012. Biotreatment of selenium refinery wastewater using pilot-scale granular sludge and swim-bed bioreactors augmented with a selenium-reducing bacterium Pseudomonas stutzeri NT-I. Japanese J. Wat. Treat. Biol. 48: 63 71. 11) Turner, R.J., A.L. Papish, and F. Sargent. 2004. Sequence analysis of bacterial redox enzyme maturation proteins (REMPs). Can. J. Microbiol. 50: 225 238. 12) 2014 43: 96 101.