111-245111-N-03 21300AMY00496000 2272 2181 1 2 3 4 5 1Mycobacteria Growth Indicator Tube 4,7 1,10 2OADC Enrichment 3 PANTAPANTA Antibiotic Mixture, Lyophilized B600g/ 4,7 1,10 7H9 1. 2 PANTA 1 PANTA 365nm UV 1 1 Centers for Disease Control and PreventionCDC Public Health Mycobacteriology. A Guide for the Level Laboratory 3, 4 N L NALC NALC/NaOH NALC/NaOH 1 2 NALC/NaOH 15 5 10 ph6.83000g 20 PBS0.6mL NALC/NaOH4% 50mL 2.9% 50mL NALC 0.5g 1
2NALC/NaOH 15 510PBSpH6.8 3,000g20 PBS0.6mL 2 10mL 3,000g 20 3 10mL 3,000g 20 5mL 1 2mL 4 3,000g 20 1 2mL 2 NALC/NaOH 15 5 10 ph6.83,000g 20 PBS0.6mL NALC/NaOH4% 50mL 2.9% 50mL NALC 0.5g 2 CDC Clinical Microbiology Procedures Handbook 3, 4, 5 3 24 4 5 NALC/NaOH 2 1 0.5mL NALC/NaOH 1 50mL 2NALC/NaOH 5mL 5 20 315 25 15 20 20 435mL ph6.8 53,000g 20 6 ph6.8 7 0.5mL PANTA 2
2 1 PANTA 1 4L 1 2 3 PANTA 3mL 2 7mL 1 2 /PANTAPANTA 15mL 2 4mL UV 365nm 7mL BD MGIT MGIT 3 1 4mL 1 PANTA 3mL 4mL 0.5mL 3mL PANTA 0.1mL NALC/NaOH 0.5mL 0.1mL 1% 372 UV 365nm PANTA3mL 4mL 0.5mL PANTA0.1mL NALC/NaOH0.5mL 37UV365nm 2 4mL 0.4% 5mL 1 22 25 2 4 2 7mL 1 7mL PANTA0.8mL NALC/NaOH 0.5mL 0.1mL 1% 2MGIT BD MGIT 3
PANTA15mL 7mLPANTA0.8mL 7mLNALC/NaOH0.5mL MGIT 1 4mL 2 UV 10 4 10 7 CFU/mL 4mL UV 365nm1 1 4 10 UV 4mL 8 2 7mL MGIT 60 42 56 3 4 5 1 1 1 1n652 247 70 2 317 1 1 334 335 248 404 652 89.1%581/652 99.6% 82.7% 2 1 1 DDH 2 70 70 40 PCR 4
70 30 DDH 2 1n68 22 1 3 23 0 45 45 22 46 68 98.5%67/68 100% 97.8% 2 3 7H11 Mycobaterium tuberculosis Complex 3 n57 13 0 13 0 44 44 13 44 57 100%57/57 100% 100% 4 n50 14 0 14 0 36 36 14 36 50 100%50/50 100% 100% 5 n50 7H11 8 0 8 0 42 42 8 42 50 100%50/50 100% 5
100% 2 24 ATCC 10 3 10 5 CFU/mL M.africanum M.gordonae M.nonchromogenioum M.terrae M.avium complex M.haemophilum M.phlei M.triviale M.chelonae M.intracellulare M.scrofulaceum M.tuberculosis M.flavescens M.kansasii M.simiae M.vaccae M.fortuitum M.malmoense M.smegmatis M.xenopi M.gastri M.marinum M.szulgai M.abscessus 1 1 6 2 Mycobacterium tuberculosis CDC 3 2 1 2 7mL 3 UV 365nm UV 4 Mycobacterium haemophilum 30 BBL X 1 MGIT 5 1 2 0.1mL 3 4 7mL 6 7 PANTA 8 2 25 8 2 8 6
PANTA 2 8 PANTA 2 8 72 206 PANTA 9 7H9 ATCC 115 7H9 2 20 3 15 4 50.5 6 1 7 1 ATCC 0.5 M.tuberculosis 27294 150 10 M.kansasii 12478 15000 11 M.fortuitum 6841 15000 3 1 10 4 10 7 CFU/mL 3 1 2 MGIT 1 234 CDC 3 4 2% ph7.5 8.5 2 25 PANTA 2 8 18 245122 7mL 7mL 100 245124 MGIT 960 PANTA 245111 4mL 4mL 25 245113 4mL 4mL 100 245114 PANTA PANTA 6 245116 6 6 6 7
1. Cohn, M. L. R. F. Waggoner, and J. K. McClatcry 1968. The 7H11 medium for the cultivation of mycobacteria. mycobacteria. Am. Rev. Resp. Dis 98. 295-296. 2. Youmans.G.P.1979.Cultivation of mycobacteria,the morphology and metabolism of mycobactera.p.25 35 Tuberculosis. W. B. SaundersCompany,Philadelphia. 3. Kent.P.T., and G.P.Kubica, 1985. Public health mycobacteriology : A guide for the level III laboratory. USDHHS, Centers for Disease Control, Atlanta 4. 2007 5. Isenberg,Henry D.1992 Clinical microbiology procedures handbook,vol.1.american Society for Microbiology. 6. Washington.D.C.Bloodborne pathogens.code of Federal Regulations.Title 29, Part 1910.1030.Federal Register 1991, 56 : 6417564182 BD BD TEL0120-8555-90FAX024-593-5761 960-2152 1 TEL0120-8555-90 8