UHPLCUHPLC Application Note CC2999 Vanquish Accucoreopiates benzodiazepines amphetamines Thermo Scientific Accucore Vanquish C18 UHPLC1.5 m Vanquish UHPLC 47 UHPLC Vanquish UHPLC Accucore Vanquish UHPLC 2 3 1 Accucore Vanquish UHPLC 1.5 m Accucore Vanquish 1.5 m Accucore Vanquish UHPLCVanquish UHPLC Vanquish UHPLC 15 MPa 15 bar
2 Accucore Vanquish C18 1.5 m UHPLC 1 2.1 mm 1711-1213 LC-MS 18 M Thermo Scientific Smart2Pure 5129845 Fisher Chemical LC-MS 1653963 Fisher Chemical analytical 155957 Fisher Chemical 159841 Thermo Scientific Virtuoso 9 mm 2 ml 618-VT4 3 47 1 mg1 ml/ 1:1, v/v 1 mg/ml / 9:1, v/v1 ng/ml Thermo Scientific Virtuoso ID Vanquish UHPLC Vanquish Vanquish Vanquish Thermo Scientific TSQ Vantage Virtuoso ID VH-P1-A VH-A1-A VH-C1-A 618-VT-1 : Thermo Scientific Xcalibur 2.1 MS control Thermo Scientific Dionex Chromeleon 7.2 LC/MS UHPLC : A: B: : : : : Accucore Vanquish C18 1.5 m 1 2.1 mm 1 mm.1% 5 L/min Forced air mode 5 2 L 1 1: % B. 1.16 1 2.88 9 3.2 9 3.28 1 5.6 1 TSQ Vantage SRM selected-reaction monitoring2 347SRM
2 : TSQ Vantage 3 HESI Positive V 45 5 Arb 75 Arb 2 38 mtorr 1. s.5 Q1 FWHM.7 Q3 FWHM.7 3: 47 SRM RF- V V min Ecgonine methyl ester 2 73 182 15.47 Normorphine 272 11 165 36.63 Dihydromorphine 288 12 185 31 1.6 Anhydroecgonine methyl ester (AEME) 182 75 91 27 1.21 Oxymorphone 32 97 161 33 1.29 Norcodeine 286 96 193 21 1.34 Morphine 286 112 21 24 1.35 Noroxycodone 32 86 187 23 1.42 Hydromorphone 286 12 185 28 1.45 Norhydrocodone 286 12 241 21 1.49 Amphetamine 136 41 91 16 1.54 MDA 18 49 163 6 1.55 Methamphetamine 15 53 91 17 1.61 O-desmethyl(-cis-) tramadol 25 72 58 16 1.62 Dihydrocodeine 32 12 21 29 1.62 N,O-didesmethyl tramadol 236 68 44 13 1.65 MDEA 28 63 163 11 1.73 Benzoylecgonine 29 95 168 17 1.75 Oxycodone 316 1 298 16 1.76 6 -acetylmorphine 328 12 165 36 1.83 Codeine 3 15 215 29 1.85 Norfentanyl 233 7 177 13 1.92 Hydrocodone 3 12 199 29 1.94 N-desmethyl tapentadol 28 75 17 23 2.5 N-desmethyl tramadol 25 59 44 14 2.6 cis-tramadol 264 75 58 16 2.6 Naltrexone 342 118 27 24 2.6 Tapentadol 222 8 17 25 2.9 Normeperidine 234 83 16 14 2.16 Meperidine 248 1 174 18 2.31 Nortilidine 26 75 77 42 2.33 Naloxone 328 91 253 25 2.36 6 -acetylcodeine 342 138 165 43 2.37
4 3: 47 SRM RF- V V min Norbuprenorphine 414 16 187 36 2.39 PCP (phencyclidine) 244 53 91 34 2.43 1,5-dimethyl-3,3-diphenylpyrrolodone (EDDP) 278 12 234 29 2.46 Clonazepam 316 95 27 23 2.66 Tilidine 274 76 155 18 2.77 Oxazepam 287 87 241 2 2.81 Methadone 31 84 265 13 2.81 Lorazepam 321 1 275 2 2.82 Alprazolam 39 11 281 24 2.84 Flurazepam 388 1 315 21 2.95 Fentanyl 337 16 188 22 2.97 Nordiazepam 271 1 14 27 2.99 11-nor- -9 -carboxy-thc 345 14 193 24 3.49 Buprenorphine 468 138 187 39 3.75 Vanquish UHPLCAccucore Vanquish UHPLC 47 6 SRM1 5 L/min 89 MPa 89 bar 123.3 MPa 1233 baraccucore Vanquish UHPLC 4,5, 4,, 3,5, 3,, Abundance 2,5, 2,, 1,5, 1,, 5, -5,.1.5 1. 1.5 2. 2.5 3. 3.5 4. 1: 47 SRM
24SRM 2m/z 28 34m/z 3 342 5 Relative Abundance 2.6 1 9 8 7 6 5 m/z 28 17 4 3 2 1 1.73 1 9 8 7 6 5 m/z 28 163 4 3 2 1 1. 1.5 2. 2.5 3. 2: N-desmethyltapentadol MDEA m/z 28 Relative Abundance 1.85 1 9 8 7 6 5 m/z 3 215 4 3 2 1.94 1 1.94 1 9 8 7 6 5 m/z 3 199 4 1.85 3 2 1 1. 1.5 2. 2.5 3. 3: codeine hydrocodone m/z 3
Relative Abundance 2.5 1 9 8 7 6 5 m/z 342 27 4 3 2 1 2.37 1 9 8 7 6 5 m/z 342 165 4 3 2 1 2.5 1. 1.5 2. 2.5 3. Application Note CC2999 4: naltrexone 6-acetylcodeine m/z 342 naltrexone Accucore Vanquish C18 1.5 m UHPLC Vanquish UHPLC 47 6 Vanquish UHPLC 1 McHale, K., Thermo Scientific, Quantitative LC-MS Screening for Illicit Drugs Using Ultrahigh Resolution Mass Analysis and Accurate Mass Confirmation, Thermo Scientific Application Note 21 AN63166 b_e8 /1 S. C214 Thermo Fisher Scientific Inc. TEL 12-753-67 FAX 12-753 -671 221-22 3-9 E151 E-mail: Analyze.jp@thermofisher.com www.thermoscientific.jp