Vol. 41, 2009 Serving Advanced Technology
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Example condition: 6kV, 120uA, 3hr Example Mehod: Back side Ar ion irradiation CP The argon ion beam is parallel to a cross section surface. Ar ion Shield Plate Si Sample CP block
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Development of cryo-em: 1 st - 7 th generations 1 st G 1986 Kyoto U 2 nd G PERI 3 rd G Kyoto U 1988 1994 Helium stage 4 th G 2001 Harima, Tokyo 5 th G Tokyo 6 th G Kyoto U 2004 2006 7 th G with U-SET system Fourier transforms of Bacteriorhodopsin images Untilted 60
(a) (b)
Mem-1 Mem-2 Mem-3 Mem-1 Mem-2 Mem-3 Mem-1 Mem-2 Mem-3
Structures of membrane proteins analyzed by cryo-electron microscope with top entry helium stage HK-ATPase MGST-1 AQP0 AQP1 Nature, 407, 599-605 (2000) Nature, 387, 624-627 (1997) br EMB J., 28, 1637-1643 (2009) Cx26 JMB, 360, 934-945 (2006) AQP4 Nature, 438, 633-683 (2005) AChR Nature, 389, 206-211 (1997) LHC PNAS, 104, 10034-10039 (2007) Nature, 458, 597-602 (2009) by X-ray JMB, 355, 628-639 (2006) Nature, 423, 949-955 (2003) Nature, 367, 614-621 (1994)
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(a) (b) (c) (d) (e) (a) (b) (c) (d)
(a) (b) (c) (d) (e) Ret(all-trans)-C 60 Ret(11-cis)-C 60 a11-trans
Fig. 8
Abundance 3000 2000 1000 0 18.0 18.2 18.4 18.6 18.8 RT 19.0 m/z 126 m/z 129 m/z 132 19.2 19.4 Abundance 3000 2000 1000 0 18.0 18.2 18.4 18.6 18.8 RT 19.0 m/z 126 m/z 129 m/z 132 19.2 19.4 S Strecker degradation oxidation C S S S H S S NH 2 methionine methional methanethiol DMTS methionine + [methyl-d 3 ]- methionine methional methanethiol + + [methyl-d 3 ]- [methyl-d 3 ]- methional methanethiol H 3 C S S S CH3 (MW 126) H 3 C S S S CD3 (MW 129) D 3 C S S S CD 3 (MW 132)
DMTS-producing potential (ng/ml) 0.5 0.4 0.3 0.2 0.1 DMTS-P1 DMTS-P2 50 40 30 20 10 Me (%) 10 8 6 4 2 Glucose (%), rganic acids (mm) 0.0 0 0 20 40 60 Fraction (10 ml/tube) 80 10 10 DMTS produced(ng/ml) 8 6 4 2 DMTS produced(ng/ml) 8 6 4 2 0 frc N DMTS-P1 DMTS-P1 frc N 0 frc N DMTS-P2 DMTS-P2 + frc N
A220 300 200 0 10 RT 20 30 40 100 DMTS producing potential (ng/ml) 0 0.00 0.01 0.02 0.03 0.04 0.05 1 234 5 6 7 8 9
S 6 5 4 3 2 1 H + H - 2 H 2 S S S H + + H H - 1,2-dihydroxy-5-(methylsulfinyl) methanesulfenicacid pentan-3-one (DMTS-P1) S S S 2 H + methanethiol 2 S S DMDS S S S DMTS
S
H 2 B A C 6' 3 (+)-Catechin (Cat) H Planar Catechin (PC1) H + Planar Catechin (PC1) G k H H + GH 3 10-3 kht, M -1 s -1 2 1 0 0 0.05 0.10 0.15 [Sc 3+ ], M
H Electron Transfer PhCMe 2 + Sc 3+ PC1 2Me 2Me H PC1 2 2 2 H 1.10G 6.60G H H H 1.10G PC1 H + PhCMe 2 Sc 3+ (a) g = 2.0048 (b) g // = 2.070 g // = 2.050 2500 g = 2.005 Proton Transfer PhCMe 2 + Sc 3+ H 5 G 50 G H 298 K 77 K
H (+)-Catechin Fe 3+ / H 2 2 Quercetin Planar Catechin Fe 3+ / H 2 2 Fe 3+ / H 2 2 1 2 3 4 5 6 7 8 9 10 11 12 13 14 (+)-Catechin H R R Lipophilic Planar Catechin (PCn) Form II Form III Form I R R R = n H n =1 9
SCEs/cell 100 80 60 40 20 0 100 80 60 40 20 0 H H H Control RESVERATRL H 4' 4' 4 0 0 0 2.5 5 10 0 2.5 5 10 0 2.5 5 10 Dose ( g / ml) H 0 2.5 5 10 100 80 60 40 20 100 80 60 40 20 0 H H 3 5 H 0 2.5 5 10 Dose ( g / ml) Resveratrol 10 g/ml 100 80 60 40 20 100 80 60 40 20 0 4' 0 2.5 5 10
+ DNA Cu(II) A Bn R 1 Bn AlCl 3 (CH 3 ) 2 NC 6 H 5 Et CH 2 P Et R 1 H H + Bn R 2 R 3 R 2 CH 1 : R 1 = H 3 : R 2 = R 3 = H 2 : R 1 = CH 3 4 : R 2 = H, R 3 = CH 3 5 : R 2 = R 3 = CH 3 NaH THF R 1 Bn Bn R 2 R 3 Bn 6 : R 1 = R 2 = H, R 3 = CH 3 7 : R 1 = H, R 2 = R 3 = CH 3 8 : R 1 = CH 3, R 2 = R 3 = H 9 : R 1 = R 2 = CH 3, R 3 = H 10 : R 1 = R 2 = R 3 = CH 3 R 3 + Resveratrol B C 11 : R 1 = R 2 = H, R 3 = CH 3 12 : R 1 = H, R 2 = R 3 = CH 3 13 : R 1 = CH 3, R 2 = R 3 = H 14 : R 1 = R 2 = CH 3, R 3 = H 15 : R 1 = R 2 = R 3 = CH 3 + d D + e E + f F 2600 2800 3000 3200 3400 3600 Gauss R 3 R 1 R 4 R 2 Resveratrol: R 1 = R 3 = R 4 =, R 2 = H d: R 1 = H, R 2 = R 3 = R 4 = e: R 1 =, R 2 = R 3 = R 4 = H f: R 2 =, R 1, R 3, R 4 = H
(a)exp. g = 2.0047 H H H 3 CH 3 C H H CH CH 3 CH 3 3 G Galvinoxyl Radical (G )) GH GH (b) Sim. 3 G H H H 3 C H 3 C H H 0.601mT 0.141mT 0.601mT H 0.141mT CH 3 H CH 3 CH 3 0.141mT CH 3 0.141mT
(a) (b) Cell with chromosome aberrations (%) 60 40 20 0 60 40 20 0 60 40 20 0 resveratrol 11 12 resveratrol 15 0 5 10 20 0 5 10 20 Concentration (µg/ml) 4-methylresveratrol (13) 14 15
CD X Axis [mm] Y Axis [mm] - -
Magnification: 150k
(0, -30mm) 26 nm 24 nm 150 nm 26 nm 26 nm (30mm, 0) Center (0, -30mm) 48 nm (0, 30mm) 48 nm 48 nm 48 nm 48 nm
E-beam resist Resist image Silicon mold Dose condition 50 nm Measurement area 50 nm including 10 10 holes ZEP520A 350 C/cm 2 D : 19.9 nm 3 : 1.6 nm 50 nm 50 nm New-CAR 100 C/cm 2 D : 22.8 nm 3 : 2.5 nm D: diameter 116 nm 36 nm
JEM-ARM200F The Power of STEM Cs corrected Microscope 78 pm real imaging by HAADF method (Pico meter order microscopy) RAW data Specimen: Si (112)single crystal Acc vol:200kv
HR-Elemental identification by EELS (Atomic column by column EELS map) HAADF signal (acquired simultaneously) RGB map Sr-M map Ti-L map -K map
JXA-8530F BEI FE LaB 6
JMS-T100GCV JSPM-5410
0 16 http://www.jeol.co.jp/ 0 1 6 1 1 1 1 1 1 0 1 6 0 1 6 0 1 6 0 1 1 1 1 1 0 1 6 0 1 6 0 1 6 0 1 6 0 1 6 0 1 6 MGMT.SYS. MGMT.SYS. RvA C024 R va C425 IS 9001 & 14001 REGISTERED FIRM DNV Certification B.V., THE NETHERLANDS No. 9301H875C 9401H969C (Kp)