user manual Chromatography
Tokyo Standard Time Copy to Metafile...
Marker Set Marker Ref. Point Max, Min, Ave
Integrate Calculate Baseline Integrate Peak Integrate Reject peaks
Edit Peak Table Edit Fill Peak...
A AKTAprime PrimeView System Wash 50ml/min Manual Run 30ml/min e-mail : Tech-JP@ge.com Home Page http://www.gehealthcare.co.jp/lifesciences
4. 4.1 p174.1 4.2 1 1 Templates Application Template System Wash Method Select Buffer V. Pos B, A:-,-,-,-,-,-,- OK Press OK to Start Run Method Complete Press OK to complete Memory Print Out? (no) yes no 2Templates OK 3Application Template OK 4 System Wash Method OK 5 B, A OK 6Press OK to Start Run OK 7Method Complete Press OK to complete OK 8Memory Print Out? no System Wash Method 1 MPa Manual Run Manual Run Set Flow Rate (1.0 ml/min) Start Run 9Manual Run OK 10) Set Flow Rate 1.0 ml/min OK 11 Start Run OK 12 0.05 MPa 0.05 MPa 4.3 4.4-2 -
4.3 ÄKTAprime Purge Kit P-95096 % 1 Waste Manual Run Set Injection Valve Pos (Waste) 2Manual Run OK 3 Set Injection Valve Pos OK Waste OK 496% 5 6 Set Flow Rate (5.0 ml/min) 7 Set Flow Rate 5.0ml/min OK Press OK to Start Run 8 Press OK to Start Run OK 9 10 11 12end 4.4 1 A1 96% Manual Run Set Flow Rate (30.0 ml/min) 2 Manual Run OK 3 Set Flow Rate 30.0ml/min OK Set Injection Valve Pos (Waste) 4 Set Injection Valve PosWaste OK Start Run 5 Start Run OK 630 ml pause/cont A1 7pause/cont 60 ml end - 3 -
4.5 p194.4 4.6 Cue Cards 1. HPLC 2. ph 0.45 * 3. 1 8 A 2 3 2 System Wash Method Manual Run Set Concentration %B (100 %B) 3 Manual Run OK 4 Set Concentration %B 100% OK Set Flow Rate (30.0 ml/min) 5 Set Flow Rate 30.0ml/min OK Set Injection Valve pos (Waste) 6 Set Injection Valve Pos Waste OK Start Run 7 Start Run OK 830 ml pause/cont Set Concentration %B (0 %B) 9 Set Concentration %B 0% OK - 4 -
10pause/cont 11 60 ml end Manual Run Set Flow Rate (1.0 ml/min) 12Manual Run OK 13) Set Flow Rate 1.0 ml/min OK Start Run 14 Start Run OK 15 0.05 MPa * 0.45 µm - 5 -
1.1.............................................................................................. 1 1.2 PrimeView............................................................................................... 3 2.1...................................................................................................... 4 2.2........................................................................................... 5 2.3 UV.................................................................................................... 7 2.4............................................................................................ 8 2.5.............................................................................................. 8 3.1...................................................................................................... 9 3.2..................................................................................................... 10 3.3................................................................................................... 11 3.4............................................................................................... 12 3.5................................................................................... 12 3.6.............................................................................................. 14 3.7................................................................................................... 15 3.8............................................................................................. 16 3.9........................................................................................................... 16 4.1.......................................................................................................... 17 4.2....................................................................................................... 17 4.3.................................................................................................. 18 4.4......................................................................................................... 19 4.5...................................................................................................... 20 4.6....................................................................................................... 20 4.7.......................................................................................... 21 5.1 Application Template............................................................................................... 22 5.2 Method Template.................................................................................................. 24 5.3...................................................................................................... 26 5.4.................................................................................................... 28 5.5...................................................................................................... 29 5.6...................................................................................................... 29 5.7.......................................................................................... 30 5.8................................................................................................. 31 5.9...................................................................................................... 31 6.1................................................................................................. 32 6.2.......................................................................................... 33 6.3......................................................................................... 34 6.4....................................................................................................... 35 7.1.................................................................................................. 37 7.2 Result file........................................................................................... 37 7.3 Result file.................................................................................................... 38 7.4...................................................................................................... 38 7.5 Export............................................................................................... 39 7.6 Metafile................................................................................................ 40 7.7............................................................................................ 41 7.8............................................................................................ 41 8.1............................................................................................................ 42 8.2........................................................................................................... 42 8.3............................................................................................... 43 8.4............................................................................................................ 44 8.5 PrimeView / PrimeView Evaluation...................................................................................... 44 9.1 Menu....................................................................................................... 45 9.2 ÄKTAprime............................................................................................ 49 9.3......................................................................................... 52 9.4............................................................................................ 54
ÄKTAprime ÄKTAprime User Manual 71-1744-32PrimeView User manual18-1150-66 ÄKTAprime System pump Mixer Sample Column Monitoring Fractionation Pressure sensor Injection valve UV/conductivity ph (optional) Flow restrictor Buffer valve Gradient switch valve Waste Flow diversion valve Ä A B Buffers Fraction collector Waste AB A81 8
0.150 ml / min1 MPa 0.2 MPa3.5 A, B2 ml 0.6 ml 0.1 5 ml / min 2 ml 1 10 ml / min 5 ml 5 30 ml / min 12 ml 15 50 ml / min 7LOAD, INJECT, WASTE3 ÄKTAprime100 µl, 500 µl, 1 ml, 5 ml4 Superloop 10, 50, 150 ml3 LOAD, position 1 INJECT, position 2 WASTE, position 3 Column Sample syringe 2 1 3 7 6 Pump Column 2 1 3 7 6 Pump Column 2 3 1 7 6 Pump 4 5 4 5 4 5 Waste Waste Waste UVUV280 nm254 nm 2 mm phph ÄKTAprime User Manual71-1744-326.5 ph 88 µlpeek 0.7510 cm44.9 µl phph 42 M KNO 3 =11 0.2 MPa 0.2 MPa 3.5
ÄKTAprime2 Logbook Logbook PrimeView ÄKTAprime
l 100 V3 ÄKTAprime 120 cm 80 cm 32 cm 25 cm 44 cm 20 cm ÄKTAprimeRS-232C1.8 m 1 2RS-232C9 ÄKTAprime 3 0 0l ÄKTAprime Drop SensorUV-lamp Frac Valve RS-232 RecorderRec. ph-ground On/off ph-probe Conductivity Mains Flow Cell UV ÄKTAprime PrimeView 1 ÄKTAprime
1 2 Pause 3 41 Tube Sensor 55 mm
6PEEK5 mm 7 PEEK Sensor Control feed tube 8feed tube 14 62 ÄKTAprime User Manual 8.13
UV254 nm280 nm 280 nm254 nm 1 2 UV11ÄKTAprime ONUV
ÄKTAprime12 ÄKTAprime User Manual 3.15 3.5 UVUV1 ÄKTAprime3.1 2.2 0.2 M
Selftest Please wait... ÄKTAprime V2.01 Templates 1ÄKTAprime ÄKTAprimeUV72 UV1ÄKTAprime 2Windows2000 3Welcome to WindowsCtrl, Alt, Del 3 4Log On to Windows Enter Windows2000 Logon 5PrimeViewPrimeView PrimeViewÄKTAprimePrimeView-prime RS-232C 6PrimeView Evaluation PrimeView PrimeView Evaluation
System Wash Method 35 35 20 % 1 Templates 2 Application template 3 System Wash Method 4 Select Buffer V. Pos B,A:-,-,-,-,-,-,- Press OK to Start RUN OK 5 B,AOK 6 20 % Method Complete Press OK to complete 75 Memory Print Out? (yes) yes no 8 9PrimeViewPrimeView Evaluation 10Windows2000 11ÄKTAprime OFF ONUV
OK Esc Main menus Sub menus Main menu 1 Press or Main menu 2 OK ESC Submenu 2.1 OK ESC Sub menu 2.1.1 Sub menu 2.2 Press or Sub menu 2.3 OK ESC Sub menu 2.3.1 Sub menu 2.3.2 feed tube end hold /cont pause /cont 1 9.1 Menu cont.4
69.1 Menu Templates Run Stored Method Manual Run Program Method Application TemplateMethod Template5.1 Application Template5.2 Method Template 5.4 3.5 Copy Method Set Parameters Check UVpH UV Manual Run Set Method Base (ml) min ml minml Set Concentration %B (20%B) 30 Conc %B B% A Conc %B 0 B Conc %B 100 AB73 Conc %B 30
Set Gradient (Off) Set Length (0.00 ml) Set Target (00 %B) ml Conc %B Set Flow Rate (0.1 ml/min) 0.1 0.150.0 ml/min Set Fraction Base (ml) drp min ml Set Fraction Size (0.00 ml) 0.2 Set Pressure Limit (1.00 MPa) 1.0 Pause0.01.0 MPa* 1 Set Buffer Valve Pos (Pos 1) Pos. 18 Set Inject Valve Pos (Load) Waste Load Inject LoadInjectWaste 1.1 Start Run Press OK to Start Run End Run? (yes) yes no Memory Print Out? (no) yes no *1 HiPrep0.15 MPa ÄKTAprime 0.35 MPa HiPrep0.15 MPaFR-9020.2 MPa 20 %
ÄKTAprime ÄKTAprime ÄKTAprime 2 Method, Application Template 5 4 Press OK to Start Run OK ÄKTAprime Memory Print out? (no) yes no C:/UNICORN/local/Fil/Prime/Result Application Template Method Template method AT2002Jul29no1.RES MT2002Aug12no3.RES 2002Aug22no6.RES ManualRun 1.RES Method Result
PrimeView User Manual p3 1 Properties 2Chromatogram LayoutCurves 3X-AxisXBaseTimeVolume
4Y-AxisY PrimeView User manual p9 2 1
13PEEK 28 38 4 26 1 1 Templates Application Template System Wash Method Select Buffer V. Pos B,A:-,-,-,-,-,-,- OK Press OK to Start Run 6 Method Complete Press OK to complete Memory Print Out? (no) yes no 8 System Wash Method 1 MPaManual Run
ÄKTAprime Purge Kit P-950 1AB Templates Templates Application template Application template System Wash Method System Wash Method Select Buffer V. Pos B,A:-,-,-,-,-,-,- Press OK to Start Run Method Complete Press OK to complete Memory Print Out? (no) yes no Manual Run Press OK to Start Run no 9Manual Run Set Injection Valve Pos (Waste) 10Set Injection Valve Pos Waste 1196% 12 13 Purge tubing connector stop plug Set Flow rate (5.0 ml/min) Start Run 14Set Flow Rate5.0ml/min 15 Press OK to Start Run 16 17 18 19 20
1 2 3 M6 female-1/16'' male * 4 5 6 1/16'' female-m6 male * Manual Run 7 Set Flow Rate (3,0 ml/min) Start run Press OK to Start Run 8 0.5 96 10 end 11 End run? (yes) yes no 12 Memory Print Out? (no) yes no 13 '' '' '' '' 722.4 UV
Cue Application TemplateSystem Wash Method 1 8 A 23 Templates 3Templates Application Template Application Template System Wash Method System Wash Method Select Buffer V. Pos B,A:-,-,-,-,-,-,- OK Press OK to Start Run µ
HiTrap Desalting HiPrep Desalting 12,000 g100.45 µm 4 feed tube 1 2feed tube
ÄKTAprime Application TemplateMethod Template Application Template5.1 Method Template5.2 Application Template 5.3 11 Templates Cue cardäktaprime User Manual 5.2 Application Template Desalting HiTrap Desalting HiTrap Desalting 5 ml Desalting HiPrep Desalting His-tag Purification HiTrap Chelating Refolding HiTrap Chelating GST-tag Purification GSTrap Mab Purification Gradient Elution Mab Purification Step Elution Albumin Removal HiTrap Blue IgM Purification HiTrap IgM Purification Anion Exchange HiTrap Q Cation Exchange HiTrap SP System Wash Method His-Tag * 1 His-Tag * 1 GST ph ph IgM HiPrep 26/10 Desalting HiTrap Chelating 1 ml HiTrap Chelating 1 ml GSTrap 1 ml HiTrap Protein G 1 ml HiTrap Protein A 1 ml HiTrap rprotein A 1 ml HiTrap Protein G 1 ml HiTrap Protein A 1 ml HiTrap rprotein A 1 ml HiTrap Blue 1 ml HiTrap IgM purification HiTrap Q 1 ml HiTrap SP 1 ml
1 2 HiTrap 35 ml 4 0.5 mlni 2+ 1 ml 5ÄKTAprime 6Application TemplatePosition 3Ni 2+ RefoldingApplication Template SS DTT 2-mercaptoethanol Templates Application template Anion Exchange HiTrap Q Sample appl. Volume 0.0 Press OK to Start RUN Memory Print Out? (no) yes no
4 140 1 Templates 2 Method template 3 Ion Exchange Gradient elution Sample inject by InjV Pump 4 5 Set Pressure Limit (1.00 MPa) 1.00 6 Set Flow Rate (--.- ml/min) 0.00 Set Fraction Size (0.0 ml) 0.0 Set Equilibr. Volume (0.0 ml) 0.0 7 8 95
Set Sample Inj. Vol. (0.0 ml) 0.0 10 Set Wash 1 Volume (0.0 ml) 0.0 1123 Set Elution. Volume (0.0 ml) 0.0 1220 Set Wash 2 Volume (0.0 ml) 0.0 135 Method ready? Press OK 14 Save Method (yes) yes no 15 Free Methods 25 Sel. Method (Free) 16 16 Free Methods 25 Sel. Method (Used) 16 17 Press OK to Start Run 18 Memory Print Out? (no) yes no 19 2
ÄKTAprime 1 40 999 2 1 2 ÄKTAprimeProgram Method5.6 ÄKTAprime40 Conc %B Templates Program Method ÄKTAprime Free Methods 25 Sel. Method (Used) 09 UsedFree Method Occupied (edit) edit clear Set Method Base (ml) 1 timeml
Set Fraction Base (ml) 2timemldrop Set Pressure Limit (1.00 MPa) 3 Pause Edit Breakpoint Edit Breakpoint New Edit New Breakpoint ml 0.0 Set Concentration %B (0 %B) Set Flow Rate (-,--ml/min) 1 2 OK Set Flow Rate (-,--ml/min) Set Flow Rate (1.0 ml/min) Set Flow Rate (-,--ml/min) Set Fraction Size (0.0 ml) Set Buffer Valve Pos (Pos1) Set Injection Valve Pos (Load) Set Peak Collect (no) Set Slope (0.00 ma/min) Autozero (no) Event Mark (no) Injection Valve INJECT Edit time/volume (0.0 ml/min) Save Breakpoint (0.0 ml) Delete Breakpoint (0.0 m) Edit time/volume Change Replace Change Replace Set Alarm at (No Alarm) Show %B on Rec out 2 (no) PrimeView Evaluation End Method Save Method PC C:/UNICORN/Bin/Prime
No. No. Templates Copy Method Copy Method From? System PC Copy Method From System No : 1 Copy Method From System No : A Copy Method To? System PC Copy Method To PC (Free) No : B Copy Method Press OK System A PC B Copy Method Method Saved! Copy Method To PC (Used) No : B Method Occupied Clear it? Yes No
ÄKTAprime40 Templates 1 Program Method 2 Free Method 3 Sel.Method (Used) 20 3 Free Occupied (edit) edit clear 4 Clear Method 20? Yes No 5 Free Method 4 Sel.Method (Free) 20 6 No. 20Free Method Template 5.3 ÄKTAprime5.4 ÄKTAprime
Run Stored Method 1 Run Stored Method From System PC 2 Run Stored Method From PC No : X 3 Press OK to Start Run 4
M RUN 10.0 ml 20.0 ml/min 1.10 MPa 0.00002 AU ph 8.50 20%B 22.90 ms/cm Cond 78.8% Tc 22.4 C Tube:01 Frac 5.0 ml Method Application templatemethod templatepc UVpH%BmS CondCond%TcTubeFrac Waste V : (waste) BV(1) IV:(waste) Waste VBV IV Set Concentration %B (20 %B) 30 Set Flow Rate (0.1 ml/min) 0.8 Set Fraction Size (00.0 ml) 0.2 Conc %B Set Buffer Valve Pos (Pos 1) 1 Set Inject Valve Pos (Load) Waste Load Injection Autozero Event Mark end pause cont hold cont Conc %B Method Complete Press OK to continue 1 Memory Print Out (no) yes no 2 End run? (no) yes no 3
PrimeView Evaluation PrimeView User Manual (18-1150-66) 1Result File Result File3.6 7.2 Result file 2 Properties
3Chromatogram LayoutCurves 4X-AxisXBaseTimeVolume 5Y-AxisYOK 1
2Tile Cascade Tile PrimeView User Manual, p24
PrimeView EvaluationLogbook 1Report 2 32
4 Setup Chromatogram PrimeView User Manual 3.5 Generating reports
1Result file 1 Open Result Create New Folder Application Template AT2002Jul29no1.RES Method Template MT2002Aug12no3.RES method 2002Aug22no6.RES 1 Result file
2 Open ResultRename 1) 2 1 2
PrimeView User Manual 3.6.9 Exporting data and curves 1 2 3 4 factor 5 6 7 8
1 2 3
1 2 3 3 1 2 Floppy (A) Zip 1 2 3
M RUN 10.0 ml 20.0 ml/min 0.5 MPa A. 4.3 Manual Run 5.8 A. 1. 2. ph A. 2 ml110 ml / min 2 Set parameter Setup and calibration Set Mixer Chamber Vol ÄKTAprime User ManualC.2.18 A. 0.45 µm System Wash Method1 M NaOH 5 ml / min30 A. UV12.3 UV UV1 M NaOH1 ml / min30 ÄKTAprime User Manual7.9 UV A. Check Check Lamp Run time 280 nmr 150 mväktaprime User Manual7.15 UV
A. 1. 2. UV 3. 1 M NaOH ÄKTAprime User Manual 7.2 A. 1 M NaOH A. 3.5 A. 8,0004 / 16 A. 1. 2. 2.2 3. A. 5.8 A. A. 4.3 A. pause / cont A. FEED A. Method Template Edit
A. A. 1. 2. 3. 20% 4. 1 M NaOH A. 1. 2. ph A. Ä A. 1 A. Manual Runs (prime) 10 A. PrimeView EvaluationReport 6.4 A. PrimeView Evaluation %B
Templates Run Stored Method Manual Run Program Method Copy Method Application template Method template Desalting HiTrap Desalting Desalting HiPrep Desalting His-tag Purification HiTrap Chelating GST-tag Purification GSTrap Mab Purification Gradient Elution Refolding HiTrap Chelating IgM Purification HiTrap IgM Purification Mab Purification Step Elution Albumin Removal HiTrap Blue Anion Exchange HiTrap Q Cation Exchange HiTrap SP Sample Appl. Volume (0.0 ml) 0.0 Set Parameters System Wash Method Check Select Buffer V. Pos B,A:-,-,-,-,-,-,- OK Press OK to Start RUN Gel filtration / Buffer Exchange Ion Exchange Gradient elution HIC Gradient elution Affinity Step Gradient Sample inject by InjV Pump Set Pressure Limit (1.00 MPa) 1.00 Set Flow Rate (01.0 ml/min) 1.0 Set Fraction Size (0.0 ml) 0.0 Set Equilibr. Volume (0.0 ml) 0.0 Set Sample Inj. Vol. (0.0 ml) 0.0 Set Wash 1 Volume 1. (0.0 ml) 0.0 Set Elution Volume (0.0 ml) 0.0 Set Wash 2 Volume 1. (0.0 ml) 0.0 1. Does not apply to Gel filtration/buffer exchange Method Ready? Press OK Save Method (yes) yes no Free Methods 25 Sel. Method (used)09 Clear Method 09 (yes) To RUN data displays and Print-out menus yes no Press OK to Start RUN TO RUN data displays and Print-out menus
Run Stored Method Run Stored Method From System PC Run Stored Method From System No. 14 Press OK to start run TO RUN data displays and Print-out menus Manual Run Set Method Base (ml) Set Concentration %B (20 %B) Set Gradient (off) Set Flow Rate (3,0 ml/min) Set Fraction Base (ml) Set Fraction Size (00.0 ml) Set Pressure Limit (1.00 MPa) Set Buffer Valve Pos (Pos 1) Set Injection Valve Pos (Load) Start run Set Length (0.00 ml) 0.0 Set Target (00 %B) 0 Press OK to start run To RUN data displays and Print-out menus Check Check communication PC App. Connected! Check Autozero AZ 0.0000AU Check Lamp Run time Hg00000h Zn00000h Check Lamp intensity R 5.5 S 5.7 mv Check Pump Run time 123456h Check Pumped Volume 123456789 ml Check Tube Shifts 123456 Check Valve Shifts BV:123456 IV:123456 Check Recorder Telephone Service: 012345678901234567 Contract Number: 012345678901 Serial Number: 01234567 YM012345 ÄKTA prime Vx.xx.xx Date of maintenance DD MMMM YYYY Buzzer Test Check service Mode
Program Method Free Methods 25 Sel. Method (Used) 09 Set Method Base (ml) Set Fraction Base (ml) Set Pressure Limit (1.00 MPa) Edit Breakpoint Set Alarm at (No alarm) Show %B on Rec out 2 (no) yes no End Method Save Method (yes) yes no Edit Breakpoint ml 5.0 Set Concentration %B (20.0 %B) Set Flow Rate (2.0 ml/min) Set Fraction Size (0.00 ml) Set Buffer Valve Pos (Pos 1) Set Inject Valve Pos (LOAD) Set Peak Collect (no) Autozero (no) Event Mark (no) Edit time/volume (0.0 ml) Save Breakpoint (0.00 ml) Delete Breakpoint (0.00 ml) Method Occupied (edit) edit clear Clear Method 09 (yes) yes no Set Slope (0.00 mau / min) 0.00 Edit time/volume Change Replace New time/volume (5.0 ml) 5.0 Run data displays 2 RUN 7.8 min 22.6 ml/min 1.00MPa 0.00002AU ph 8.50 20%B 22.90 ms/cm Cond 78.8%Tc 22.4 C Tube:01 Frac 5.0 ml Waste V:(waste) BV(1) IV:(waste) 3. 1. Set Concentration %B (20.0 %B) Set Gradient Set Flow Rate (2.0 ml/min) Set Fraction Size (0.00 ml) Set Buffer Valve Pos (Pos 1) Set Inject Valve Pos (LOAD) Set Peak Collect 2. (no) Autozero (no) Event Mark (no) 2. Method Complete Press OK to complete Print-out menus Memory Print Out? (yes) yes no Set Rec Out 1 (UV) UV ph Cond %B Tmp Pr Set Rec Out 2 (%B) UV ph Cond %B Tmp Pr Set Rec Out 3 (Cond) UV ph Cond %B Tmp Pr Autoscaling (no) Press OK to start Printing Printing Please wait... End run? (no) yes no Run finished 1. Applies to Manual Run ONLY. 2. Does NOT apply to Manual Run. 3. Does NOT apply to Application Template.
Set Parameters Print out to Recorder Computer Printing Out? Method Loggdata Lamp (on) Set Drop Sync Active (yes) Memory Print out Setup Analogue Out Setup and Calibration Set Rec Out 1 (UV) UV ph Cond %B Tmp Pr Set Rec Out 2 (%B) UV ph Cond %B Tmp Pr Set Rec Out 3 (Cond) UV ph Cond %B Tmp Pr Autoscal UV (no) Press OK to start Printing Printing Please wait... Set Rec Out 1 (UV) UV ph Cond %B Tmp Pr Set Rec Out 2 (%B) UV ph Cond %B Tmp Pr Set Rec Out 3 (Cond) UV ph Cond %B Tmp Pr Set UV Analogue Out (0.0005AUFS 10.0%) Set Cond Analog. Out (00.00-50.00 ms/cm) Set ph Analog Out (ph 12.00-3.00) Set Press. Analog. Out (1.00 MPa) Set UV Zero Level (10.0%) Set UV Range (0.0005AUFS) Set Cond Zero Level (0.00 ms/cm) Set Cond Full Scale (50.00 ms/cm) Set ph Zero Level (ph0.00) Set ph Full Scale (ph 13.00) Calibrate ph (7.00-10.00) Set ph Temp Comp (off) Set Show ph (on) Calibr ph Buffer 1 Calibr ph Buffer 2 Calibrated Electrode Slope 100.0% 12.5 mv Setup ph Setup Cond Setup UV Setup Temp Start Pump Calibr. (683 Pulses) Change Press Offset (1005 mv) Set Delay UV to Frac. (380 µl) Set Mixer Chamber Vol. (2.0 ml) Set Cond Temp Comp (0.0%) Set Cond Ref Temp (25.0 C Set Adjust Cond (83.56 ms/cm) Set Adj Cell Const (83.56 cm-1) Set Show Cod (on) Set Averaging (1.3 s) Set Lamp Rum Time Hg (2000 h) Set Show UV (on) Set Adjust Temp (25.0 C) Set Show Temp (on) Pump Calibrated OK Press OK to continue. Set Flow Rate (3.0 ml/min) 3.0 Enter Collected Volume 0.00 ml Set zero pressure to calib. Press OK Calibrating Offset Done! Press OK
9.2 ÄKTAprime HiTrap rprotein A FF 51 ml/21 ml 17-5079-01 / 17-5079-02 15 ml 17-5080-01 HiTrap Protein A HP 51 ml/21 ml 17-0402-01 / 17-0402-03 15 ml 17-0403-01 HiTrap Protein G HP 51 ml/21 ml 17-0404-01 / 17-0404-03 15 ml 17-0405-01 HiTrap IgM Purification HP 51 ml 17-5110-01 HiTrap IgY Purification HP 15 ml 17-5111-01 HiTrap Blue HP 51ml/15ml 17-0412-01 / 17-0413-01 HiTrap Benzamidine FFhigh sub 51ml/21ml 17-5143-01 / 17-5143-02 15ml 17-5144-01 HiTrap Streptavidin HP 51ml 17-5112-01 HiTrap Heparin HP 51ml/15ml 17-0406-01 / 17-0407-01 HiTrap Chelating HP 51ml/15ml 17-0408-01 / 17-0409-01 GSTrap FF GST 51ml/21ml 17-5130-01 / 17-5130-02 15ml 17-5131-01 HiTrap NHS-activated HP 51ml/15ml 17-0716-01 / 17-0717-01 HiTrap IEX Selection Kit 7HiTrap 1 ml Fast Flow 17-6002-33 1mlFF Q XL / Q / ANX / DEAE / SP XL / SP / CM HiTrap Fast Flow IEX Selection Kit JP 5HiTrap 5 ml Fast Flow 90-1002-00 5mlFF SP / CM / Q / DEAE / HiTrap Desaltin HiTrap SP XL 51ml/55ml 17-5160-01 / 17-5161-01 SP XLSP FF, SP HP SP HPSP XL, SP FF HiTrap SP FF 51ml/55ml 17-5054-01 / 17-5157-01 HiTrap SP HP 51ml/55ml 17-1151-01 / 17-1152-01 HiTrap CM FF 51ml/55ml 17-5056-01 / 17-5155-01 HiTrap Q XL 51ml/55ml 17-5158-01 / 17-5159-01 Q XLQ FF, Q HP QHPQ XL, Q FF HiTrap Q FF 51ml/55ml 17-5053-01 / 17-5156-01 HiTrap Q HP 51ml/55ml 17-1153-01 / 17-1154-01 HiTrap ANX FFHigh sub 51ml/55ml 17-5162-01 / 17-5163-01 HiTrap DEAE FF 51ml/55ml 17-5055-01 / 17-5154-01 HiTrap HIC Selection Kit 5HiTrap 1 ml 17-1349-01 1ml Butyl / Octyl / Phenyllow sub,high sub/ Phenyl HP HiTrap Phenyl FFhigh sub 51ml/55ml 17-1355-01 / 17-5193-01 HiTrap Phenyl FFlow sub 51ml/55ml 17-1353-01 / 17-5194-01 HiTrap Phenyl HP 51ml/55ml 17-1351-01 / 17-5195-01 HiTrap Octyl FF 51ml/55ml 17-1359-01 / 17-5196-01 HiTrap Butyl FF 51ml/55ml 17-1357-01 / 17-5197-01 HiTrap Desalting 55ml 17-1408-01
HiPrep 16/10 Heparin FF 1.610 cm 17-5189-01 HiPrep 16/10 SP XL 1.610 cm 17-5093-01 HiPrep 16/10 SP FF 1.610 cm 17-5192-01 HiPrep 16/10 CM FF 1.610 cm 17-5091-01 HiPrep 16/10 Q XL 1.610 cm 17-5092-01 HiPrep 16/10 Q FF 1.610 cm 17-5190-01 HiPrep 16/10 ANX FF high sub 1.610 cm 17-5191-01 HiPrep 16/10 DEAE FF 1.610 cm 17-5090-01 HiPrep 16/10 Phenyl FF (high sub) 1.610 cm 17-5095-01 HiPrep 16/10 Phenyl FF (low sub) 1.610 cm 17-5094-01 HiPrep 16/10 Octyl FF 1.610 cm 17-5097-01 HiPrep 16/10 Butyl FF 1.610 cm 17-5096-01 HiPrep 16/60 Sephacryl S-100 HR 1.660 cm 17-1165-01 HiPrep 26/60 Sephacryl S-100 HR 2.660 cm 17-1194-01 HiPrep 16/60 Sephacryl S-200 HR 1.660 cm 17-1166-01 HiPrep 26/60 Sephacryl S-200 HR 2.660 cm 17-1195-01 HiPrep 16/60 Sephacryl S-300 HR 1.660 cm 17-1167-01 HiPrep 26/60 Sephacryl S-300 HR 2.660 cm 17-1196-01 HiPrep 16/10 Desalting 1.610 cm 17-5087-01 Ä
HiLoad 16/10 SP Sepharose HP 1.610 cm 17-1137-01 HiLoad 26/10 SP Sepharose HP 2.610 cm 17-1138-01 HiLoad 16/10 Q Sepharose HP 1.610 cm 17-1064-01 HiLoad 26/10 Q Sepharose HP 2.610 cm 17-1066-01 RESOURCE S, 1 ml 0.463 cm 17-1178-01 RESOURCE S, 6 ml 0.643 cm 17-1180-01 RESOURCE Q, 1 ml 0.463 cm 17-1177-01 RESOURCE Q, 6 ml 0.643 cm 17-1179-01 RESOURCE ETH, 1 ml 0.463 cm 17-1184-01 RESOURCE ETH, 6 ml 0.643 cm 17-1422-10 RESOURCE ISO, 1 ml 0.463 cm 17-1185-01 RESOURCE ISO, 6 ml 0.643 cm 17-1422-11 RESOURCE PHE, 1 ml 0.463 cm 17-1186-01 RESOURCE PHE, 6 ml 0.643 cm 17-1422-12 HiLoad 16/60 Superdex 30 pg 1.660 cm 17-1139-01 HiLoad 26/60 Superdex 30 pg 2.660 cm 17-1140-01 HiLoad 16/60 Superdex 75 pg 1.660 cm 17-1068-01 HiLoad 26/60 Superdex 75 pg 2.660 cm 17-1070-01 HiLoad 16/60 Superdex 200 pg 1.660 cm 17-1069-01 HiLoad 26/60 Superdex 200 pg 2.660 cm 17-1071-01 RESOURCE1.5 MPaÄKTAprime1.0 MPa 1.0 MPa
1SuperloopGlass tube(7) Movable seal(6), Inner end piece(3) 2Inner end piece (18-1112-5718-1112- 55)1 3Movable seal Glass tube 2 4Glass tubemovable seal 23 5Glass tube Inner end piece Glass tube 6Superloop5)Inner end piece 7Outer end pieceprotective jacket 1Superloop6 26 2 Inject 3 1 ml / min 4Movable seal ml 5
1 2 3 4 5 6
Item Quant./pack A/C* Code no. Optical unit Hg lamp & housing complete 1 C 18-1128-22 Zn lamp & housing complete 1 C 18-1128-23 UV flow cell 5 mm 1 C 18-1128-24 UV flow cell 2 mm 1 C 18-1128-25 Filter 214 nm 1 C 18-0622-01 Filter 254 nm 1 C 18-0620-01 Filter 280 nm 1 C 18-0621-01 Filter 313 nm 1 C 18-0623-01 Filter 365 nm 1 C 18-0624-01 Filter 405 nm 1 C 18-0625-01 Filter 436 nm 1 C 18-0626-01 Filter 546 nm 1 C 18-0627-01 Filter wheel complete 1 A 18-0647-01 ph electrode ph electrode, round tip, incl. flow cell and holder 1 C 18-1134-84 ph electrode, round tip 1 C 18-1111-26 ph flow cell, round tip,incl.dummy electrode 1 A 18-1112-92 Dummy electrode, round tip 1 A 18-1111-92 Mixer Mixing chambers: 0.6 ml 1 A 18-1118-90 2 ml 1 A 18-1118-91 5 ml 1 A 18-1118-92 12 ml 1 A 18-1118-93 Injection fill port 0.7 mm 1 C 18-1127-66 Fraction collector Tube racks, complete with bowl, tube support, holder and guide: 12 mm 1 A 19-8684-03 18 mm 1 A 18-3050-03 30 mm 1 A 18-1124-67 Tube support, Tube holder and guide: 1 A 18-3054-02 12 mm 1 A 19-7242-02 18 mm 1 A 19-8689-02 30 mm 1 A 18-1124-68 Eppendorf tube holder for 12 mm rack 100 A 18-8522-01 Flow diversion valve, FV-903 incl. mounting bracket 1 A 18-1114-50 Tubing holder 1 A 18-6464-01 Drive sleeve 5 C 19-6067-02
Item Quant./pack A/C* Code no. Sample loop 10 µl 1 C 18-1120-39 100 µl 1 C 18-1113-98 500 µl 1 C 18-1113-99 1 ml 1 C 18-1114-01 2 ml 1 C 18-1114-02 5 ml 1 C 18-1140-53 Superloop 10 ml, 50 ml Superloop 10 ml, completeäktadesign 1 A 18-1113-81 Superloop 50 ml, complete 1 A 18-1113-82 Inner end piece 1 A 19-7846-01 Outer end piece 1 A 19-5167-01 O-ring, inner end piece 5 C 19-7595-01 O-ring,movable seal 2 C 18-1104-97 Movable seal 1 A 19-7845-01 Protective jacket (50 ml) 1 A 19-7849-01 Glass tube with thread and groove (10 ml) 1 A 19-7593-01 Glass tube with thread abd groove (50 ml) 1 A 19-5165-01 Tubing kit for Superloop (10 ml) 1 A 18-1113-83 Tubing kit for Superloop (50 ml) 1 A 18-1113-84 Superloop 150 ml Superloop 150 ml, complete 1 A 18-1023-85 Movable seal 1 A 18-1029-58 Inner end piece 1 A 18-1029-59 O-ring, inner end piece 2 C 18-1029-60 O-ring, movable seal 1 C 18-1134-49 Cables Mains cable, 115 V 1 A 19-2447-01 Mains distribution lead 0.3 m 1 A 18-1119-05 Connectors and unions Tubing connector, inlet nut for o.d. 3/16'', PEEK 10 A 18-1112-49 Ferrule, for 3/16'' o.d. tubing, PEEK 10 A 18-1112-48 Union, 1/16'' female/m6 male, PEEK 6 A 18-1112-57 Union, Iuer female/1/16'' male, PEEK 2 A 18-1112-51 Union, M6 female/1/16'' male, PEEK 8 A 18-1112-58 Union, 1/16'' male/1/16'' male, for 1/16'' o.d. tubing, PEEK 10 A 18-1120-92 Union, 1/16'' female/1/16'' female, for 1/16'' o.d. tubing, titanium 1 A 18-3855-01 Fingertight connector 1/16'', for PEEK tubing o.d. 1/16'' 10 A 18-1112-55 Stop plug, 1/16'', PEEK 5 A 18-1112-52 Stop plug, 5/16'', PEEK 5 A 18-1112-50 *) A = accessory, C = consumable
Item Quant./pack A/C* Code no. Tubing Teflon tubing, i.d. 2.9 mm, o.d. 3/16'' (IN ) 3 m A 18-1112-47 PEEK tubing, i.d. 0.50 mm, o.d. 1/16'' (Orange) 2 m A 18-1113-68 PEEK tubing, i.d. 0.75 mm, o.d. 1/16'' (Green) 2 m A 18-1112-53 PEEK tubing, i.d. 1.0 mm, o.d, 1/16'' (White) 2 m A 18-1115-83 Sample tubing kit 1 A 18-1115-77 Inlet filter assembly 1 A 18-1113-15 Inlet filter set 10 C 18-1114-42 On-line filter 1 A 18-1112-44 On-line filter kit 10 C 18-1027-11 Flow restrictor, FR-902 1 A 18-1121-35 Flow restrictor, FR-904 1 A 18-1119-63 Column holder, for one column, short 1 A 18-1113-17 Column holder, for one column, long 1 A 18-1126-32 Flow cell holder for optical unit 1 A 18-3055-87 Clamp, conductivity flow cell 1 A 18-1111-14 Tubing cutter 1 A 18-1112-46 U-wrench, M6 1 A 19-7481-01 U-wrench, 1/4'' 1 A 18-1112-45 Allen key, 2.5 mm 1 A 19-4442-01 Chart recorder REC-112, 2 channel 1 A 18-1132-33 User Documentation ÄKTAprime User Manual 18-1135-24 Cue cards Mab purification, step elution 18-1138-01 His-tag Purification 18-1138-02 Desalting on HiTrap Desalting 18-1138-03 Desalting on HiPrep Desalting 18-1138-04 Cation Exchange 18-1138-05 GST-tag Purification 18-1138-06 Anion exchange 18-1139-39 Mab Purification, gradient elution 18-1139-41 IgM Purification 18-1139-42 Refolding 18-1139-43 Albumin removal 18-1139-44 Method Templates value table 18-1139-45 *) A = accessory, C = consumable
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