RIKEN Symposium MHC Dynamics and functions of the MHC genes of animals from fish to human- Friday, Apl. 15, 2005 10:00-17:10 Okochi Hall, RIKEN (The Institute of Physical and Chemical Research) Contact: Retrovirus Research Unit (Tel. 048-462-4420) ******************************************************************************** MHC MHC MHC HLA HLA 230 6 HLA 1,200 100 MHC MHC MHC ********************************************************************************
1 - (30 ) (20 ) (20 ) Genomic Organizations of the Major Histocompatibility Complex in Cat and Dog (30 ) Naoya Yuhki (National Cancer Institute USA) ( 2 ) MHC ( ) 2 - (15 ) (15 ) (15 ) 3 MHC (25 ) (25 ) (25 ) (25 ) 2
4 (20 ) (15 ) 5 (30 ) 1,000 500 3
4
Major Histocompatibility Complex MHC HLA Human Leukocyte Antigen T HLA 6 6p21.3 3.6 Mb HLA HLA-A -B -C I HLA-DR -DQ -DP II HLA I II TNF III HLA HLA 100 MHC HLA HLA paralogous 1q21-22 4.7 Mb HLA 3.6 Mb 239 88 HLA 45 ( HLA 70 TNF ) 194 15.1 kb HLA 6 HLA 1,400 HLA MHC 1q21-22 19p13 9q33-34 HLA 1q21-22 19p13 9q33-34 paralogous HLA 30 kb HLA HLA HLA 100 HLA 5
100 kb 30,000 100 kb MHC 10 Mb MHC HLA MHC MHC MHC MHC MHC 4 5 MHC 6p21.3 1q21-22 19p13 9q33-34 4 paralogous 6p21.3 MHC MHC T MHC MHC MHC T adaptive immunity 6
MHC (Artiodactyla) 6000 90 4 MHC MHC Bovine Leukocyte Antigen BoLA 23 BoLA DO DY DM TAP LMP II IIb DR, DQ MHC IIa MHC (OLA) MHC (GoLA) II BoLA II (BLV) BLV T (HTLV) B (EBL) BLV (PL) EBL BoLA-DRB3 BLV BLV BoLA II II EBL 7
- - MHC (SLA) SLA SLA SLA SLA DNA SLA SLA INRA Sanger Inst. 2.4 Mb SLA - MHC MHC MHC SLA I SLA I I HLA I MHC SLA SBT PCR-RFLP PCR-SSP 4 100 SLA I II I α1, α2 SLA I HLA I DNA SLA 5 2 SLA SLA 8
Genomic Organizations of the Major Histocompatibility Complex in Cat and Dog NAOYA YUHKI Laboratory of Genomic Diversity, National Cancer Institute at Frederick Abstract: We have established gene organization and a draft sequence of domestic cat MHC, using methods of RH mapping, FISH, BAC/PAC mapping and shotgun sequencing. This study showed that unlike a majority of mammalian MHCs so far studied, the domestic cat MHC has a split genomic structure, one including extended class II, classical class II, class III and proximal class I regions which localize on peri-centromeric region of a long arm of chromosome B2 and other including a distal class I and extended class I regions which localize on a telomeric region of a short arm of the same chromosome B2. Previous comparative study indicated that the cat chromosome B2 was rearranged from the syntenic human chromosome 6 by two chromosome inversions, one including the entire short arm and other including a distal portion of the long arm. Our results support this study and further suggest that a breakpoint of the short arm inversion resides on the MHC. Based on recently established two supercontig sequences for canine MHC assembled in the canine WGS sequencing project, we concluded that canine MHC also has the split gene organization as seen in the domestic cat MHC, however, one segment -extended class II, classical class II, class III and proximal class I regions localizes on chromosome 12 and other -distal class I and extended class I localizes on chromosome 35. Since the split in both feline and canine MHCs were found between TRIM39 and TRIM26 genes, we concluded that this MHC structure occurred as a single event before the split of feline and canine species, was maintained through species evolution and influenced unique gene organization of class I genes in both MHCs. 9
Procyon lotor 1970 1994 5 33 mtdna 7 13 5 20km MHC PrLA-DRB1 10
MHC 9,000 15,500 13,500 5,000 (Major Histocompatibility Complex ; MHC) T MHC MHC MHC DNA MHC II 2-3 11
MHC MHC MHC I Ia (Onmy-UBA) Ia α3 α1 α2 18 kbp Ia Ia α1 6 α2 3 α3 3 α1 UBA 5 Ib Onmy-UCA UDA UEA UFA UGA UBA Ia 18 Ib 14 MHC Ι (Ia Ib) Ia MHC I Ia IHN Ia Ia Ib IPN 12
MHC MHC T Tcr (Ig) MHC Tcr Ig Tcr Ig MHC MHC Tcr MHC MHC MHC MHC MHC 13
MHC genome paralogy MHC MHC 1 9 19 MHC MHC MHC VLR (variable lymphocyte receptor) Max Cooper, Zeev Pancer, Chris Amemiya VLR 14
β α 15
MHC MHC MHC MHC 15 16.5 Mb MHC MHC birth and death MHC 16
M-integra imanishi@jbirc.aist.go.jp MHC MHC M-integra MHC ortholog M-integra M-integra web G-compass 82 17 G-compass H-InvDB G-compass http://www.jbirc.aist.go.jp/g-compass/ G-compass MHC MHC 17 MHC MHC 17
18
MHC MHC MHC II MHC MHC FeLV FIV FIP MHC MHC FLA- DRB 2 DNA MHC MHC 19
バイオテクノロジー分野において高品質な製品を提供しています 事業内容バイオ関連 理化学研究機器 一般試薬の販売 取扱品目機器 / 光分析 分離分析 解析 汎用 研究実験設備試薬 / 分子生物 生化学 組織培養 免疫学 一般試薬 レノバサイエンス株式会社 本 社 113-0033 東京都文京区本郷 3 44 2 多摩営業所 185-0011 東京都国分寺市本多 5 5 1 横浜営業所 230-0051 神奈川県横浜市鶴見区鶴見中央 2-6-5 宇都宮営業所 321-0953 栃木県宇都宮市東宿郷 3-6 -1 金沢営業所 920-0058 石川県金沢市示野中町 1 36 TEL 03-3818-8980 FAX 03-3818-4870 TEL 042-320-3861 FAX 042-320-3864 TEL 045-508-1733 FAX 045-508-1722 TEL 028-651-4157 FAX 028-651-4158 TEL 076-232-7332 FAX 076-232-7844