130503090505080209020508070506000301010808 0906020902010608, 2016, ｨｰ06ｨｬ 51, 01 3, 09. 327-334 070208 633.111.1:577.152.31:577.151.64 doi: 10.15389/agrobiology.2016.3.327rus 06050208030400030106010407 080108090605 06050603040805 00 050403090409 05030003010809 01030805080309020602010207 000801080207 03010301060004 (Triticum aestivum L.) 08.05. 0407020808020008 1, 05.00. 04070208080200 1, 01.00. 0208000402020008 2, 01.00. 00060405080808 3, 03.00. 040704080009030008 2, 07.00. ﾁ03050102080200 4 0609ｨｰ0208ｨ 0404 ｪ ｨ 0605ｨｹ03ｨ 07 0008ｨｮ0707ｨ 000208ｨｬ02ｨｪｨｰ0609, ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｴｨｨ01 08ｨ 09ｨｴ02070502ｨｪｨｨ02 09050603ｨｪ060500ｨｨ08ｨｪ0401 0909070402ｨｦ. 02ｨ 04 ﾂｨｪ06 ｨｨ01 08ｨ 040102050706ｨｰ ｨｪｨ ﾂ02ｨｰ040802 ｨｰｨｨ07ｨ : 010605ｨｨｨｪ0509ｨｰ0208ｨ 0404 (ｨｪｨ ｨｨｨ 06050202 ﾂｨ 09ｨｰ06 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ06ｨｰ0907 0708ｨｨ ｨｰ08ｨ 01ｨｨ02ｨｨ06ｨｪｨｪ06ｨｬ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺ06ｨｬ ｨ ｨｪｨ 05ｨｨ0402), ｨ 0202ｨｰｨｨ050509ｨｰ0208ｨ 0404, ｨ 08ｨｨ050509ｨｰ0208ｨ 0404 ｨｨ ｨｺｨ 08ｨ 06ｨｺ09ｨｨ050509ｨｰ0208ｨ 0404. 0609ｨｰ0208ｨ 0404 08ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｰ 0708020908ｨ ｨｴ02ｨｪｨｨ02 ｨｺｨ 08ｨ 06ｨｺ09ｨｨ05ｨｹｨｪ0401 0500ｨｨ080609 09 ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0402 ｨｺｨｨ090506ｨｰ04 ｨｨ 0907ｨｨ08ｨｰ04, ｨ 05ｨ 000601ｨ 0807 ﾂ02ｨｬｨｮ ｨｨ0008ｨ 06ｨｰ ｨｺ0506 ﾂ0209ｨｮ06 080605ｨｹ 0906 ｨｬｨｪ0600ｨｨ01 ｨ ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 07080602020909ｨ 01. 02ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ02 0507ｨｨ09ｨｰｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨｨｦ ｨｨ ｨｺ060106ｨｬｨｨｨｪｨ ｨｪｨｰｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｪｨ 090502010609ｨ ｨｪｨｨ07 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 010205ｨ 06ｨｰ ｨｨ01 04ｨｪｨ ﾂｨｨｨｬ04ｨｬｨｨ 010507 ｨ 0409ｨｰ08060006 ｨｨ 010609ｨｰｨｮ07ｨｪ060006 ｨｨ04ｨｮ ﾂ02ｨｪｨｨ07 070806020209090609 ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨｨｬ0907 ｨｮ09050609ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ 0908020104. 06ｨｰ06ｨｰ ｨｰｨｨ07 ｨｬｨ 08ｨｺ02080609 ｨｮ0106ｨ 02ｨｪ ｨｨ 010507 08020302ｨｪｨｨ07 0708ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ01 04ｨ 01ｨ ﾂ 09020502ｨｺ02ｨｨｨｨ 09 ｨｺｨ ﾂ0209ｨｰ0902 0908020109ｨｰ09ｨ, 0907060906ｨ ｨｪ060006 ｨｮ09ｨｺ0608ｨｨｨｰｨｹ ｨｨ ｨｮ07080609ｨｰｨｨｨｰｨｹ 07080602020909 06ｨｰｨ 0608ｨ 09020502ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ. 000205ｨｹ06 ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ04 ｨ 0405ｨ 060202ｨｪｨｺｨ ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ060006 07080600ｨｨ0507 0509ｨｰ0208ｨ 04, 090401020502ｨｪｨｪ0401 ｨｨ04 040802050401 0902ｨｬ07ｨｪ, ｨｨ ｨｮ09ｨｰｨ ｨｪ06090502ｨｪｨｨ02 070605ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｬ020301ｨｮ 06ｨ 08ｨ 0402ｨ ｨｬｨｨ 070208090702ｨｺｨｰｨｨ09ｨｪ060006 09020502ｨｺ02ｨｨ06ｨｪｨｪ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｮ 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ 070302ｨｪｨｨ0204 (Triticum aestivum L.) 0706 05ｨｰ06ｨｬｨｮ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬｨｮ ｨｬｨ 08ｨｺ0208ｨｮ. 0609090502010609ｨ 05ｨｨ 040802050402 0902ｨｬ02ｨｪｨ 090608ｨｰ0609 070302ｨｪｨｨ0204 09020502ｨｺ02ｨｨｨｨ 000609ｨｺ060909ｨｺ060006 010606 090205ｨｹ09ｨｺ060006 01060407ｨｦ09ｨｰ09ｨ 000102ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03 (000609ｨｺ060909ｨｺｨ 07 06ｨ 05.) ｪ 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ (070806090402) ｨｨ 000208ｨ (0604ｨｨｨｬ04ｨｦ); 09020502ｨｺ02ｨｨｨｨ 0800080600ｨｨ04ｨｨ ﾂ0209ｨｺ060006 010606 (080806, 00. 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800) ｪ 05ｨｨｨｪｨｨｨｨ 08080691 ｨｨ 080806177 (070806090402), ｨ ｨｰｨ ｨｺ0302 0802ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ 080201ｨｪ0401 05ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ 070607ｨｮ050702ｨｨｨｨ ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89 ｨｨ 115 (070806090402). 0502ｨｬ02ｨｪｨ 08ｨ 04ｨｬｨ 050409ｨ 05ｨｨ 09 00ｨ 08000608060906ｨｦ 09ｨｰｨｮ07ｨｺ02, ｨｬｨｮｨｺｨｮ 0708060902ｨｨ09ｨ 05ｨｨ ﾂ02080204 09ｨｨｨｰ06. 0306090502 05ｨｺ09ｨｰ08ｨ ｨｺ02ｨｨｨｨ 000208ｨｬ02ｨｪｨｰ0609 06ｨ 08ｨ 040204 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ 010609ｨ 0106 ﾂｨｪｨｮ06 03ｨｨ01ｨｺ0609ｨｰｨｹ, 04ｨ ｨｰ02ｨｬ 0609ｨｮｨｴ0209ｨｰ09050705ｨｨ 090208ｨｰｨｨｨｺｨ 05ｨｹｨｪ04ｨｦ ｨｪｨ ｨｰｨｨ09ｨｪ04ｨｦ 050502ｨｺｨｰ08060006080204. 00 ｨｺｨ ﾂ0209ｨｰ0902 08ｨ 040102050706ｨｴ020006 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 8 % 070605ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ 000205ｨｹ, 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨｨ08ｨｮ06ｨｴ020006 ｪ 4 % 070605ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ 000205ｨｹ. 00060502ｨｺｨｮ050708ｨｪ04ｨｬｨｨ ｨｬｨ 08ｨｺ0208ｨ ｨｬｨｨ 0905ｨｮ03ｨｨ05ｨｨ 06ｨｺ08ｨ 0302ｨｪｨｪ0402 09ｨｰｨ ｨｪ01ｨ 08ｨｰ04 Page Ruler Prestained Protein Ladder (00Thermo Scientific03, 09ｨｨｨｰ09ｨ ). 0306 06ｨｺ06ｨｪ ﾂｨ ｨｪｨｨｨｨ 050502ｨｺｨｰ08060006080204ｨ 000205ｨｹ 06ｨ 08ｨ ｨ ｨ ｨｰ0409ｨ 05ｨｨ 0802ｨ ｨｺｨｰｨｨ0906ｨｬ ｨｪｨ ｨｪ0209070202ｨｨ00ｨｨ ﾂ0209ｨｺｨｮ06 0509ｨｰ0208ｨ 04ｨｮ. 05ｨｺｨ ｨｪｨｨ080609ｨ 05ｨｨ 070605ｨｮ ﾂ02ｨｪｨｪ0402 050502ｨｺｨｰ0806000608020008ｨ ｨｬｨｬ04, 060202ｨｪｨｨ09ｨ 05ｨｨ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｦ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ 07080600ｨｨ05ｨｹ ｨｺｨ 0301060006 06ｨ 08ｨ 0402ｨ, 08ｨ 0909 ﾂｨｨｨｰ0409ｨ 05ｨｨ 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｨ 0202 01ｨｨ0907020809ｨｨ06. 0609ｨｰ0208ｨ 04ｨｪ04ｨｦ ｨｺ06ｨｬ070502ｨｺ09 09 ｨｨ09090502010609ｨ ｨｪｨｪ0401 0902ｨｬ02ｨｪｨ 01 070302ｨｪｨｨ0204 ｨ 0405 0708020109ｨｰｨ 090502ｨｪ 10 000608ｨｬｨ ｨｬｨｨ. 07 090608ｨｰ0609 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ 000208ｨ 09040709ｨｨ05ｨｨ 06ｨｰ 9 0106 10 ｨｨ0406000608ｨｬ 09 08ｨ 04ｨｪ06ｨｦ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺ06ｨｦ 07060109ｨｨ03ｨｪ0609ｨｰｨｹ06, ｨｮ 08080691 06ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 7, 09 080806177 ｪ 8, ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI ｪ 06ｨｰ 7 0106 10 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04. 020507 09090201 06ｨ 08ｨ 04020609 ｨ 040506 01ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 Est-8, Est-9 ｨｨ Est-10. 0102ｨｰ0208060002ｨｪｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｰ0605ｨｹｨｺ06 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｨ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ 090609ｨｰｨ 09ｨｮ 0509ｨｰ0208ｨ 04 09 ｨ 0605ｨｹ0302ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ 090906ｨｦ ｪ Est-1, Est-2, Est-3, Est-4, Est-5, Est-6 ｨｨ Est-7. 08ｨ 030104ｨｦ ｨｨ04 18 090608ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ 06ｨ 05ｨ 01ｨ 05 06ｨｰ05ｨｨ ﾂｨｪ04ｨｬ 06ｨｰ 0108ｨｮ00ｨｨ01 06ｨ 08ｨ 04020609 0002ｨｪ06ｨｰｨｨ0706ｨｬ. 05080201ｨｪ0707 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ (H) 06ｨ 08ｨ 04020609 0706 10 090407090502ｨｪｨｪ04ｨｬ 0506ｨｺｨｮ09ｨ ｨｬ, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨｨｬ ｨｨ0406000608ｨｬ04 0509ｨｰ0208ｨ 04, 090609ｨｰｨ 09ｨｨ05ｨ 0,924; 01ｨｨ0907020809ｨｨ07 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ 010507 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ060006 ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ 04020609 Var(H) = 0,0004. 00 080204ｨｮ05ｨｹｨｰｨ ｨｰ02 ｨｨ09090502010609ｨ ｨｪｨｨｨｦ 090401020502ｨｪ04 ｨｪｨ ｨｨｨ 06050202 070208090702ｨｺｨｰｨｨ09ｨｪ0402 09020502ｨｺ02ｨｨ06ｨｪｨｪ0402 080601ｨｨｨｰ0205ｨｹ09ｨｺｨｨ02 000608ｨｬ04 ｪ 090608ｨｰｨ 0505ｨ ｨｰｨ, 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ 08080691, 080806177, ITMI7, ITMI44, ITMI83 ｨｨ ITMI115. 01ｨ 05ｨｨ ﾂｨｨ02 10 ｨｨ0406000608ｨｬ 010205ｨ 02ｨｰ 0509ｨｰ0208ｨ 0404 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ 070302ｨｪｨｨ0204 ｨｮ0106ｨ ｨｪ04ｨｬ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨｨｬ ｨｬｨ 08ｨｺ020806ｨｬ ｨｨ 0706040906050702ｨｰ 070806090601ｨｨｨｰｨｹ ｨｺｨ ｨｺ 00ｨｨ04ｨｨ0605060006-ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ02, ｨｰｨ ｨｺ ｨｨ 0002ｨｪ02ｨｰｨｨｨｺ06-09020502ｨｺ02ｨｨ06ｨｪｨｪ0402 ｨｨ09090502010609ｨ ｨｪｨｨ07 06ｨ 08ｨ 04020609 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ ｨｬ0700ｨｺ06ｨｦ 070302ｨｪｨｨ0204. 080506 ﾂ02090402 09050609ｨ : 0002ｨｺ09ｨ 070506ｨｨ01ｨｪｨ 07 ｨｬ0700ｨｺｨ 07 070302ｨｪｨｨ02ｨ, 040802050402 0902ｨｬ02ｨｪｨ, ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04, 0509ｨｰ0208ｨ 0404. 0805ｨ 0909ｨｨ ﾂ0209ｨｺｨ 07 0002ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺｨ 07 060202ｨｪｨｺｨ 09020502ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ 09ｨｰｨ ｨｪ0609ｨｨｨｰ0907 090902 ｨｬ02ｨｪ0202 0904000601ｨｪ06ｨｦ 09 ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02ｨｬ ﾂｨｨ0905ｨ ｨｺ06050502ｨｺ02ｨｨｨｦ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 080209ｨｮ08090609 08ｨ 09ｨｰ02ｨｪｨｨｨｦ, 070609ｨｺ0605ｨｹｨｺｨｮ ｨｰ0802ｨ ｨｮ02ｨｰ 090802ｨｬ02ｨｪｨｪ0401 ｨｨ 0102ｨｪ0203ｨｪ0401 04ｨ ｨｰ08ｨ ｨｰ, ｨ 070605ｨｮ ﾂ02ｨｪｨｪ0402 010507 0002ｨｪ06ｨｰｨｨ07ｨ 080204ｨｮ05ｨｹｨｰｨ ｨｰ04 09ｨ 08ｨｹｨｨ08ｨｮ06ｨｰ 0708ｨｨ 090408ｨ ｨｴｨｨ09ｨ ｨｪｨｨｨｨ 09 08ｨ 0405ｨｨ ﾂｨｪ0401 ｨｮ09050609ｨｨ0701. 08ｨｪｨ 05ｨｨ04 ｨｪｨ 0609ｨｪ0609ｨ ｨｪｨｨｨｨ 070605ｨｨｨｬ060800ｨｪ060006 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺ060006 090702ｨｺｨｰ08ｨ ｨ 0205ｨｺ06090401 ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ0401 ｨｬｨ 08ｨｺ02080609 ｪ 05ｨｰ06 ｨ 06050202 010203020904ｨｦ 070601010601, ｨｺ06ｨｰ060804ｨｦ 09 ｨｮ0907020106ｨｬ ｨｬ060302ｨｰ 0708ｨｨｨｬ02ｨｪ07ｨｰｨｹ0907 010507 05ｨｺ090708020909-060202ｨｪｨｺｨｨ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 080209ｨｮ08090609 08ｨ 09ｨｰ02ｨｪｨｨｨｦ, ｨｮ09ｨｰｨ ｨｪ06090502ｨｪｨｨ07 00ｨｨ05060002ｨｪ02ｨｰｨｨ- ﾂ0209ｨｺｨｨ01 06ｨｰｨｪ060302ｨｪｨｨｨｦ ｨｬ020301ｨｮ 08ｨ 04ｨｪ04ｨｬｨｨ 09ｨｨ01ｨ ｨｬｨｨ ｨｨ 090407090502ｨｪｨｨ07 010802ｨｦ00ｨ 0002ｨｪ0609 ｨｨ05ｨｨ ｨｨｨｪ0401 0002ｨｪ02ｨｰｨｨｨｺ06-050906050602ｨｨ06ｨｪｨｪ0401 070806020209090609 ｨｪｨ 070607ｨｮ050702ｨｨ06ｨｪｨｪ06ｨｬ ｨｮ080609ｨｪ02 (1-5). 327
130609ｨｰ0208ｨ 0404 ｪ ｨ 0605ｨｹ03ｨ 07 0008ｨｮ0707ｨ 000208ｨｬ02ｨｪｨｰ0609 (ｨｪ02 ｨｬ02ｨｪ0202 20), ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｴｨｨ01 08ｨ 09ｨｴ02070502ｨｪｨｨ02 09050603ｨｪ060500ｨｨ08ｨｪ0401 0909070402ｨｦ. 00 03ｨｨ0806ｨｺ06ｨｬ 09ｨｬ04090502 ｨｺ 0509ｨｰ0208ｨ 04ｨ ｨｬ 06ｨｰｨｪ060907ｨｰ0907 05ｨｨ07ｨ 0404, 00060900ｨ ｨｰｨ 0404, 09ｨｮ05ｨｹ00ｨ ｨｰｨ 0404 ｨｨ 0906ｨ 09ｨｰ0902ｨｪｨｪ06 0509ｨｰ0208ｨ 0404. 030609050201ｨｪｨｨ02 09ｨｺ0506 ﾂｨ 06ｨｰ 09 0902ｨ 07 ｨｬｨｪ060006 ﾂｨｨ090502ｨｪｨｪ0402 09070202ｨｨ00ｨｨ ﾂ0209ｨｺｨｨ02 000208ｨｬ02ｨｪｨｰ04: 010605ｨｨｨｪ0509ｨｰ0208ｨ 04ｨｮ, 010506080600ｨｨ0505ｨ 04ｨｮ, ｨｰｨ ｨｪｨｪｨ 04ｨｮ, 0702ｨｺｨｰｨ 04ｨｮ ｨｨ 0108. 02ｨ 04 ﾂｨｪ06 0509ｨｰ0208ｨ 0404 08ｨ 040102050706ｨｰ ｨｪｨ ﾂ02ｨｰ040802 ｨｰｨｨ07ｨ : 010605ｨｨｨｪ0509ｨｰ0208ｨ 0404 070802010706 ﾂｨｨｨｰｨ 06ｨｰ 04ｨ 08070302ｨｪｨｪ0402 09ｨｮｨ 09ｨｰ08ｨ ｨｰ04 (0500ｨｨ0804 010605ｨｨｨｪｨ 09 ｨｮｨｺ09ｨｮ09ｨｪ06ｨｦ, 07080607ｨｨ06ｨｪ060906ｨｦ ｨｨ05ｨｨ ｨｬｨ 090507ｨｪ06ｨｦ ｨｺｨｨ090506ｨｰｨ ｨｬｨｨ), 05ｨｰ06ｨｰ ｨｰｨｨ07 0509ｨｰ0208ｨ 04 ｨｪｨ ｨｨｨ 06050202 ﾂｨ 09ｨｰ06 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｰ0907 0708ｨｨ ｨｰ08ｨ 01ｨｨ02ｨｨ06ｨｪｨｪ06ｨｬ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺ06ｨｬ ｨ ｨｪｨ 05ｨｨ0402; ｨ 0202ｨｰｨｨ050509ｨｰ0208ｨ 0404 ｨｨ09070605ｨｹ04ｨｮ06ｨｰ 09 ｨｺｨ ﾂ0209ｨｰ0902 09ｨｮｨ 09ｨｰ08ｨ ｨｰ0609 ｨ 05ｨｨ00ｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ02 ｨｮ0005020906010608060104, 09 ﾂｨ 09ｨｰｨｪ0609ｨｰｨｨ ｨｮｨｺ09ｨｮ09ｨｪｨｮ06 ｨｺｨｨ090506ｨｰｨｮ; ｨ 08ｨｨ050509ｨｰ0208ｨ 0404 ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ06ｨｰ0907 0902080609060106080601ｨｪ04ｨｬｨｨ ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨ ｨｬｨｨ, 09ｨｮｨ 09ｨｰ08ｨ ｨｰｨ ｨｬｨｨ 010507 ｨｪｨｨ01 0905ｨｮ03ｨ ｨｰ ｨ 0806ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ02 ｨｮ0005020906010608060104; ｨｺｨ 08ｨ 06ｨｺ09ｨｨ050509ｨｰ0208ｨ 0404 070802010706 ﾂｨｨｨｰｨ 06ｨｰ 0500ｨｨ0804 ｨ 05ｨｨ00ｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 (010609ｨｰｨ ｨｰ06 ﾂｨｪ06 000105ｨｨｨｪｨｪ040103) 09060201ｨｨｨｪ02ｨｪｨｨｨｦ, ｨｨｨｪ00ｨｨｨ ｨｨ08ｨｮ06ｨｰ0907, ｨｺｨ ｨｺ ｨｨ 010605ｨｨｨｪ0509ｨｰ0208ｨ 0404, 000609000608060800ｨ ｨｪｨｨ ﾂ0209ｨｺｨｨｨｬｨｨ 0902ｨｴ0209ｨｰ09ｨ ｨｬｨｨ (6-9). 0609ｨｰ0208ｨ 0404 08ｨ 09ｨｰ02ｨｪｨｨｨｦ (EC 3.1.1.X) ｪ 05ｨｰ06 08ｨ 04ｨｪ0609ｨｨ01ｨｪ0609ｨｰｨｹ 0509ｨｰ0208ｨ 04, 0708ｨｨ09ｨｮｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨｮ 0906ｨｨ, 070302ｨｪｨｨ0204, 0906080006, 08ｨｨ09ｨ ｨｨ 0108ｨｮ00ｨｨ01 09ｨｨ010609 (6, 10, 11). 02ｨｪｨｨ ｨｺｨ ｨｰｨ 05ｨｨ04ｨｨ08ｨｮ06ｨｰ 0708020908ｨ ｨｴ02ｨｪｨｨ02 ｨｺｨ 08ｨ 06ｨｺ09ｨｨ05ｨｹｨｪ0401 0500ｨｨ080609 09 ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0402 ｨｺｨｨ090506ｨｰ04 ｨｨ 0907ｨｨ08ｨｰ04, ｨ 05ｨ 000601ｨ 0807 ﾂ02ｨｬｨｮ ｨｨ0008ｨ 06ｨｰ ｨｺ0506 ﾂ0209ｨｮ06 080605ｨｹ 0906 ｨｬｨｪ0600ｨｨ01 ｨ ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 07080602020909ｨ 01, ｨｪｨ 0708ｨｨｨｬ0208 09 ｨ ｨｺｨｰｨｨ09ｨ 02ｨｨｨｨ 09ｨｨ00ｨｪｨ 05ｨｹｨｪ0401 ｨｬ060502ｨｺｨｮ05 (12) ｨｨ 080200ｨｮ050702ｨｨｨｨ ｨ ｨｨ06ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ 05ｨｪ01060002ｨｪｨｪ0401 07080601ｨｮｨｺｨｰ0609 (13), 09ｨｺ0506 ﾂｨ 07 00ｨｨ01080605ｨｨｨｰｨｨ ﾂ0209ｨｺ0602 08ｨ 09ｨｴ02070502ｨｪｨｨ02 0500ｨｨ08ｨｪ0401 0909070402ｨｦ 070209ｨｰｨｨ02ｨｨ010609 ｨｨ 0108ｨｮ00ｨｨ01 ｨｺ0902ｨｪ06ｨ ｨｨ06ｨｰｨｨｨｺ0609 (6). 06ｨ ｨｺ, 000208ｨ ｨｨ02ｨｨ0104 ｨｰｨｨｨ 040607ｨｨ08, 01ｨｨｨｺ0506000607ｨｬ02ｨｰｨｨ05 ｨｨ ｨ 0806ｨｬ06ｨｺ09ｨｨｨｪｨｨ0506ｨｺｨｰｨ ｨｪ06ｨ ｨｰ, ｨ ｨｰｨ ｨｺ0302 00ｨｮｨｪ00ｨｨ02ｨｨ01 ｨ ｨｨｨｪｨ 07ｨ ｨｺ08ｨｨ05 ｨｨｨｪｨ ｨｺｨｰｨｨ09ｨｨ08ｨｮ06ｨｰ0907 0102ｨｦ09ｨｰ09ｨｨ02ｨｬ 0509ｨｰ0208ｨ 04 (6, 14). 0102ｨｺ09ｨ 070506ｨｨ01ｨｪｨ 07 070302ｨｪｨｨ02ｨ (Triticum aestivum L.) ｪ 0601ｨｨｨｪ ｨｨ04 0906040102050409ｨ 02ｨｬ0401 09ｨｨ010609, 010507 ｨｺ06ｨｰ06080401 ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨｪ04ｨｦ 090609ｨｰｨ 09 0509ｨｰ0208ｨ 04 ｨ 0405 ｨｨ04ｨｮ ﾂ02ｨｪ, ｨｪ06 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ ｨｺ06ｨｪｨｰ080605ｨｹ ｨｨ01 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ 0106 09ｨｨ01 070608 070605ｨｪ0609ｨｰｨｹ06 ｨｪ02 ｨｮ09ｨｰｨ ｨｪ06090502ｨｪ. 0207ｨｨ09ｨ ｨｪ04 ﾂ02ｨｰ040802 ｨ 0506ｨｺｨ 0006ｨｬ0206050600ｨｨ ﾂｨｪ0401 0506ｨｺｨｮ090609, ｨｺ06ｨｪｨｰ080605ｨｨ08ｨｮ06ｨｴｨｨ02 0509ｨｰ0208ｨ 0404 09 08ｨ 0405ｨｨ ﾂｨｪ0401 060800ｨ ｨｪｨ 01 ｨｨ ｨｰｨｺｨ ｨｪ0701 070302ｨｪｨｨ0204. 0102ｨｪ04 0509ｨｰ0208ｨ 04 ｨｪ02040802050401 0902ｨｬ07ｨｪ, ｨｺ0608ｨｪ02ｨｦ, 05ｨｨ09ｨｰ06090401 0705ｨ 09ｨｰｨｨｨｪ06ｨｺ ｨｨ 04020502ｨｪ0401 05ｨｨ09ｨｰｨｹ0209 08ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ080609ｨ ｨｪ04 ｨｪｨ 3AS, 3BS ｨｨ 3DS 070502 ﾂｨ 01 010806ｨｬ060906ｨｬ (15-17). 0906ｨｺｨｮ0904 0509ｨｰ0208ｨ 04 05ｨｰｨｨ0605ｨｨ080609ｨ ｨｪｨｪ0401 ｨｺ0605020607ｨｰｨｨ0502ｨｦ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪ04 ｨｪｨ 3AL, 3BL, 3DL, 7BS ｨｨ 7DS (18), 05ｨｰｨｨ0605ｨｨ080609ｨ ｨｪｨｪ0401 05ｨｨ09ｨｰｨｹ0209 ｪ ｨｪｨ 6AL, 6BL ｨｨ 6DL (18-20), 040802050401 0902ｨｬ07ｨｪ ｪ ｨｪｨ 0105ｨｨｨｪｨｪ0401 070502 ﾂｨ 01 010806ｨｬ060906ｨｬ 3AL, 3BL ｨｨ 3DL (20-22). 0609ｨｰ0208ｨ 0404 070302ｨｪｨｨ0204 09 090606ｨｰ0902ｨｰ09ｨｰ09ｨｨｨｨ 0906 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｪ06ｨｦ ｨｺ06ｨｬ070604ｨｨ02ｨｨ02ｨｦ ｨ 0405ｨｨ 0607ｨｨ09ｨ ｨｪ04 ｨｺｨ ｨｺ ｨｬ06ｨｪ06ｨｬ020804 ｨｨ05ｨｨ 01ｨｨｨｬ020804 (15, 18, 19). 02ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ02 0507ｨｨ09ｨｰｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨｨｦ ｨｨ ｨｺ060106ｨｬｨｨｨｪｨ ｨｪｨｰｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｪｨ 090502010609ｨ ｨｪｨｨ07 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 010205ｨ 06ｨｰ ｨｨ01 04ｨｪｨ ﾂｨｨｨｬ04ｨｬｨｨ 010507 ｨ 0409ｨｰ08060006 ｨｨ 010609ｨｰｨｮ07ｨｪ060006 ｨｨ04ｨｮ ﾂ02ｨｪｨｨ07 070806020209090609 ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨｨｬ0907 ｨｮ09050609ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ 0908020104. 06ｨｰ06ｨｰ ｨｰｨｨ07 ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ01 ｨｬｨ 08ｨｺ02080609 ｨｮ0106ｨ 02ｨｪ ｨｨ 010507 08020302ｨｪｨｨ07 0708ｨ ｨｺｨｰｨｨ ﾂ0209ｨｺｨｨ01 04ｨ 01ｨ ﾂ 09020502ｨｺ02ｨｨｨｨ 09 ｨｺｨ ﾂ0209ｨｰ0902 0908020109ｨｰ09ｨ, 0907060906ｨ ｨｪ060006 ｨｮ09ｨｺ0608ｨｨｨｰｨｹ ｨｨ ｨｮ07080609ｨｰｨｨｨｰｨｹ 07080602020909 06ｨｰｨ 0608ｨ 09020502ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ. 00 ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ02 ｨｬ04 09070208090402 070806090205ｨｨ ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 080701ｨ 05ｨｨｨｪｨｨｨｦ ｨｨ 090608ｨｰ0609 0708060906ｨｦ ｨｨ 0604ｨｨｨｬ06ｨｦ 070302ｨｪｨｨ0204, ﾂｨｰ06 070604090605ｨｨ0506 06070802010205ｨｨｨｰｨｹ 090604ｨｬ0603ｨｪ0402 080601ｨｨｨｰ0205ｨｹ09ｨｺｨｨ02 000608ｨｬ04 010507 070609050201ｨｮ06ｨｴ020006 070605ｨｮ ﾂ02ｨｪｨｨ07 ｨｨ09010601ｨｪ060006 ｨｨ 00ｨｨｨ 08ｨｨ01ｨｪ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ 09 09020502ｨｺ02ｨｨ06ｨｪｨｪ0401 0708060008ｨ ｨｬｨｬｨ 01, ｨｪｨ 0708ｨ 090502ｨｪｨｪ0401 ｨｪｨ 070609040302ｨｪｨｨ02 ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090609ｨｰｨｨ ｨｨ 07080601ｨｮｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｮ Triticum aestivum L. 000205ｨｹ06 08ｨ ｨ 06ｨｰ04 ｨ 0405ｨ 060202ｨｪｨｺｨ ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ060006 07080600ｨｨ0507 0509ｨｰ0208ｨ 04, 090401020502ｨｪｨｪ0401 ｨｨ04 040802050401 0902ｨｬ07ｨｪ, ｨｨ ｨｮ09ｨｰｨ ｨｪ06090502ｨｪｨｨ02 070605ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｬ020301ｨｮ 06ｨ 08ｨ 0402ｨ ｨｬｨｨ 070208090702ｨｺｨｰｨｨ09ｨｪ060006 09020502ｨｺ02ｨｨ06ｨｪｨｪ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｮ 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ 070302ｨｪｨｨ0204 0706 05ｨｰ06ｨｬｨｮ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬｨｮ ｨｬｨ 08ｨｺ0208ｨｮ. 0002ｨｰ0601ｨｨｨｺｨ. 00ｨ ｨｰ0208ｨｨｨ 0506ｨｬ 010507 ｨｨ09090502010609ｨ ｨｪｨｨｨｦ 0905ｨｮ03ｨｨ05ｨｨ 040802050402 0902ｨｬ02ｨｪｨ 090608ｨｰ0609 070302ｨｪｨｨ0204 (Triticum aestivum L.) 09020502ｨｺ02ｨｨｨｨ 000609ｨｺ060909ｨｺ060006 010606 090205ｨｹ- 328
1309ｨｺ060006 01060407ｨｦ09ｨｰ09ｨ 000102ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03 ｪ 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ (070806090402) ｨｨ 000208ｨ (0604ｨｨｨｬ04ｨｦ); 09020502ｨｺ02ｨｨｨｨ 0800080600ｨｨ04ｨｨ ﾂ0209ｨｺ060006 010606 (080806) ｪ 05ｨｨｨｪｨｨｨｨ 08080691 ｨｨ 080806177 (070806090402), ｨ ｨｰｨ ｨｺ0302 0802ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ 080201ｨｪ0401 05ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ 070607ｨｮ050702ｨｨｨｨ ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89 ｨｨ 115 (070806090402). 0502ｨｬ02ｨｪｨ ｨｰｨｴｨ ｨｰ0205ｨｹｨｪ06 08ｨ 04ｨｬｨ 050409ｨ 05ｨｨ 09 00ｨ 08000608060906ｨｦ 09ｨｰｨｮ07ｨｺ02, ｨｬｨｮｨｺｨｮ 0708060902ｨｨ09ｨ 05ｨｨ ﾂ02080204 09ｨｨｨｰ06 (ｨｬ02ｨｪ0202 200 ｨｬｨｺｨｬ). 06ｨｺ09ｨｰ08ｨ ｨｺ02ｨｨ06 000208ｨｬ02ｨｪｨｰ0609 070806090601ｨｨ05ｨｨ 0708ｨｨ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802 8-12 1 7C 09 ｨｰ02 ﾂ02ｨｪｨｨ02 ｨｪ06 ﾂｨｨ, 090606ｨｰｨｪ060302ｨｪｨｨ02 ｨｬｨｮｨｺｨ :ｨ ｨｮ000208 ｪ 1:2. 00 ｨｺｨ - ﾂ0209ｨｰ0902 ｨ ｨｮ000208ｨ ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 0,005 00 0608ｨｨ09-0105l (0801 8,3), 0,001 M MgCl 2, 10 % 0005ｨｨ020208ｨｨｨｪ. 06ｨｺ09ｨｰ08ｨ ｨｺｨｰ04 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 5 ｨｬｨｨｨｪ 0708ｨｨ 15000 06ｨ /ｨｬｨｨｨｪ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ 010609ｨ 0106 ﾂｨｪｨｮ06 03ｨｨ01ｨｺ0609ｨｰｨｹ. 05ｨ ｨｰ02ｨｬ 0609ｨｮｨｴ0209ｨｰ09050705ｨｨ 090208ｨｰｨｨｨｺｨ 05ｨｹｨｪ04ｨｦ ｨｪｨ ｨｰｨｨ09ｨｪ04ｨｦ 050502ｨｺｨｰ08060006080204 (23). 00 ｨｺｨ ﾂ0209ｨｰ0902 08ｨ 040102050706ｨｴ020006 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 8 % 070605ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ 000205ｨｹ, 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨｨ08ｨｮ06ｨｴ020006 ｪ 4 % 070605ｨｨｨ ｨｺ08ｨｨ05ｨ ｨｬｨｨ01ｨｪ04ｨｦ 000205ｨｹ. 060502ｨｺｨｰ08060006080204 070806090601ｨｨ05ｨｨ 09 ｨｺｨ ｨｬ020802 Mini-PROTEAN Tetra System (00Bio-Rad Laboratories, Inc.03, 050108) 0708ｨｨ 50 ｨｬ08/ ﾂ ｨｨ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802 5-8 1 705. 00060502ｨｺｨｮ050708ｨｪ04ｨｬｨｨ ｨｬｨ 08ｨｺ0208ｨ ｨｬｨｨ 0905ｨｮ03ｨｨ05ｨｨ 0708020106ｨｺ08ｨ 0302ｨｪｨｪ0402 09ｨｰｨ ｨｪ01ｨ 08ｨｰ04 Page Ruler Prestained Protein Ladder (00Thermo Scientific03, 09ｨｨｨｰ09ｨ ). 01ｨ 0601ｨｪｨｮ 0106080603ｨｺｨｮ 00020507 09ｨｪ0609ｨｨ05ｨｨ 10-15 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ ｨ 0205ｨｺｨ (ｨ 0204 ｨｺ08ｨ 09ｨｨｨｰ020502ｨｦ ｨｨ 0108ｨｮ00ｨｨ01 ｨ 0002ｨｪｨｰ0609), 0706 06ｨｺ06ｨｪ ﾂｨ ｨｪｨｨｨｨ 050502ｨｺｨｰ08060006080204ｨ 000205ｨｹ 06ｨ 08ｨ ｨ ｨ ｨｰ0409ｨ 05ｨｨ 0802ｨ ｨｺｨｰｨｨ0906ｨｬ ｨｪｨ ｨｪ0209070202ｨｨ00ｨｨ ﾂ0209ｨｺｨｮ06 0509ｨｰ0208ｨ 04ｨｮ. 020507 05ｨｰ060006 000205ｨｹ 090401020803ｨｨ09ｨ 05ｨｨ 09 09090203020708ｨｨ0006ｨｰ06090502ｨｪｨｪ06ｨｬ 08ｨ 09ｨｰ09060802 ｨｺ08ｨ 09ｨｨｨｰ020507 09 ｨｰ02 ﾂ02ｨｪｨｨ02 5-10 ｨｬｨｨｨｪ 0106 070607090502ｨｪｨｨ07 ｨｺ0608ｨｨ ﾂｨｪ02090401 0406ｨｪ. 0808ｨ 09ｨｨｨｰ0205ｨｹ 010507 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 0404 0006ｨｰ0609ｨｨ05ｨｨ 09050201ｨｮ06ｨｴｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ: 0,1 00 1-ｨｪｨ 00ｨｰｨｨ05ｨ 0202ｨｰｨ ｨｰｨ (00Sigma-Aldrich03, 010902ｨｦ02ｨ 08ｨｨ07) 08ｨ 09ｨｰ0906080705ｨｨ 09 5 ｨｬ05 70 % 05ｨｰｨ ｨｪ0605ｨ, 04ｨ ｨｰ02ｨｬ ｨｬ02010502ｨｪｨｪ06, 0708ｨｨ 07020802ｨｬ0203ｨｨ09ｨ ｨｪｨｨｨｨ 0905ｨｨ09ｨ 05ｨｨ 09 15 ｨｬ05 0,1 00 00060900ｨ ｨｰｨｪ060006 ｨ ｨｮ000208ｨ (0801 6,0); 0,25 00 09ｨｨｨｪ020006 070806 ﾂｨｪ060006 Fast Blue RR (00Aldrich03, 050108) 08ｨ 09ｨｰ0906080705ｨｨ 09 4 ｨｬ05 07080607ｨ ｨｪ0605ｨ ｨｨ 15 ｨｬ05 0,1 00 00060900ｨ ｨｰｨｪ060006 ｨ ｨｮ000208ｨ (0801 6,0). 02ｨ ｨ 08ｨ 09ｨｰ090608ｨ 09ｨｬ0203ｨｨ09ｨ 05ｨｨ (1:1), 00ｨｨ05ｨｹｨｰ080609ｨ 05ｨｨ ﾂ02080204 09ｨｺ05ｨ 01 ﾂｨ ｨｰ04ｨｦ ｨ ｨｮｨｬｨ 03ｨｪ04ｨｦ 00ｨｨ05ｨｹｨｰ08 ｨｨ 0908ｨ 04ｨｮ 0302 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 010507 07080607090502ｨｪｨｨ07 0406ｨｪ 09 0509ｨｰ0208ｨ 04ｨｪ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 (24). 0306090502 ｨｰ060006 ｨｺｨ ｨｺ 0406ｨｪ04 09ｨｰｨ ｨｪ0609ｨｨ05ｨｨ09ｨｹ ｨｰ02ｨｬｨｪ04ｨｬｨｨ, ｨｨ04ｨ 04ｨｰ06ｨｺ ｨｺ08ｨ 09ｨｨｨｰ020507 ｨｮ01ｨ 050705ｨｨ, 070806ｨｬ0409ｨ 07 000205ｨｹ 7 % ｨｮｨｺ09ｨｮ09ｨｪ06ｨｦ ｨｺｨｨ090506ｨｰ06ｨｦ. 030605ｨｮ ﾂ02ｨｪｨｪ0402 050502ｨｺｨｰ0806000608020008ｨ ｨｬｨｬ04 09ｨｺｨ ｨｪｨｨ080609ｨ 05ｨｨ ｨｪｨ Epson Expression 10000 XL (00GE Healthcare03, 050108). 06ｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｦ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺｨｨｨｦ 07080600ｨｨ05ｨｹ ｨｺｨ 0301060006 06ｨ 08ｨ 0402ｨ 060202ｨｪｨｨ09ｨ 05ｨｨ 09 0706ｨｬ06ｨｴｨｹ06 0708060008ｨ ｨｬｨｬ04 Phoretix 1D (00TotalLab, Ltd.03, 000205ｨｨｨｺ06ｨ 08ｨｨｨｰｨ ｨｪｨｨ07). 04ｨ 09 ﾂ02ｨｰ 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ 0202 01ｨｨ0907020809ｨｨｨｨ 070806090601ｨｨ05ｨｨ 0706 000608ｨｬｨｮ05ｨ ｨｬ, 0708020105060302ｨｪｨｪ04ｨｬ 00. Nei (25) ｨｨ 09. 0002ｨｦ08 (26), ｨ ｨ 04ｨｨ08ｨｮ0709ｨｹ ｨｪｨ 0607ｨｨ09ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 08ｨ ｨｪ0202 ｨ 05000608ｨｨｨｰｨｬｨ 01 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｦ 060202ｨｪｨｺｨｨ 09ｨｰ020702ｨｪｨｨ 070605ｨｨｨｬ060800ｨｨ04ｨｬｨ (27). 040204ｨｮ05ｨｹｨｰｨ ｨｰ04. 030605ｨｮ ﾂ02ｨｪｨｨ02 ｨｨ 0607ｨｨ09ｨ ｨｪｨｨ02 05ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ 070607ｨｮ050702ｨｨｨｨ ITMI ｨ 0405ｨｨ 0607ｨｮｨ 05ｨｨｨｺ0609ｨ ｨｪ04 ｨｪｨ ｨｬｨｨ 08ｨ ｨｪ0202 (28-30). 0207ｨｨ09ｨ ｨｪｨｨ02 ｨｨ 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨｨ 090608ｨｰ0609 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ 000208ｨ 0708ｨｨ09020102ｨｪ04 09 090606ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨ ｨ 04ｨ 01 01ｨ ｨｪｨｪ0401 010609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 08020209ｨｰ08ｨ 0408. 09ｨｨｨｪｨｨ07 08080691 ｨ 0405ｨ 070605ｨｮ ﾂ02ｨｪｨ 07060908020109ｨｰ0906ｨｬ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ060006 06ｨｰｨ 0608ｨ 09 ｨｺ06ｨｬｨ ｨｨｨｪｨ 02ｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ07 090608ｨｰ0609 0708060906ｨｦ ｨｬ0700ｨｺ06ｨｦ 070302ｨｪｨｨ0204 Asakaze komugi 05 0902ｨｪｨｨｨｪ0008ｨ 0109ｨｺｨ 07 08ｨ ｨｪｨｪ0707. 09ｨｨｨｪｨｨ07 09ｨｺ06080609070205ｨ 07, ｨｪｨｨ04ｨｺ0608060905ｨ 07, 070605ｨｮ0609ｨｰｨｨ09ｨｰｨｨ09ｨｰｨ 07. 080608ｨｬｨ ｨｺｨｮ09ｨｰｨ 050802ｨｺｨｰ06ｨｨ01ｨｪｨ 07. 09ｨｨ09ｨｰ0609ｨ 07 0705ｨ 09ｨｰｨｨｨｪｨｺｨ 0105ｨｨｨｪｨｪｨ 07 ｨｨ ｨｮ04ｨｺｨ 07. 0806050609 07ｨｨ08ｨ ｨｬｨｨ01ｨ 05ｨｹｨｪ04ｨｦ, 070506ｨｰｨｪ0609ｨｰｨｹ 09080201ｨｪ0707, 0105ｨｨｨｪｨ ｨｺ06050609ｨ 09080201ｨｪ0707, ﾂｨｨ090506 04020802ｨｪ 09 ｨｺ0605060902 ｨｬｨ 050602. 00ｨ 0909ｨ 040208ｨｪｨ 09 ｨｺ06050609ｨ ｨｨ04ｨｬ02ｨｪ0702ｨｰ0907 06ｨｰ ｨｬｨ 0506ｨｦ 0106 09080201ｨｪ02ｨｦ, 040208ｨｪ06 ｨｬ0205ｨｺ0602, ｨｮ0105ｨｨｨｪ02ｨｪｨｪ0602, 06ｨｺ08ｨ 09ｨｺｨ ｨｺ08ｨ 09ｨｪｨ 07. 09ｨｨｨｪｨｨ06 080806177 070605ｨｮ ﾂｨｨ05ｨｨ ｨｨｨｪ01ｨｨ09ｨｨ01ｨｮｨ 05ｨｹｨｪ04ｨｬ 06ｨｰｨ 060806ｨｬ 09 ｨｺ06ｨｬｨ ｨｨｨｪｨ 02ｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ07 090608ｨｰ0609 0708060906ｨｦ ｨｬ0700ｨｺ06ｨｦ 070302ｨｪｨｨ0204 Asakaze komugi 05 Sonora 64. 09ｨｨｨｪｨｨ07 09ｨｺ06080609070205ｨ 07, 070605ｨｮｨｺｨ 0805ｨｨｨｺ0609ｨ 07, 070605ｨｮ0609ｨｰｨｨ09ｨｰｨｨ09ｨｰｨ 07. 080608ｨｬｨ ｨｺｨｮ09ｨｰｨ ｨｪｨ ｨｺ0506ｨｪｨｪｨ 07. 09ｨｨ09ｨｰ0609ｨ 07 0705ｨ 09ｨｰｨｨｨｪｨｺｨ 09080201ｨｪ0707 0706 0105ｨｨｨｪ02 ｨｨ 03ｨｨ08ｨｨｨｪ02. 0806050609 07ｨｨ08ｨ ｨｬｨｨ01ｨ 05ｨｹｨｪ04ｨｦ, 070506ｨｰｨｪ0609ｨｰｨｹ 09080201ｨｪ0707, 0105ｨｨｨｪｨ ｨｺ06050609ｨ 09080201ｨｪ0707, ﾂｨｨ090506 04020802ｨｪ 09 ｨｺ0605060902 09080201ｨｪ0202. 00ｨ 0909ｨ 040208ｨｪｨ 09 ｨｺ06050609ｨ ｨｨ04ｨｬ02ｨｪ0702ｨｰ0907 06ｨｰ ｨｬｨ 0506ｨｦ 0106 09080201ｨｪ02ｨｦ, 040208ｨｪ06 09080201ｨｪ0202, 06ｨｺ08ｨ 09ｨｺｨ ｨｺ08ｨ 09ｨｪｨ 07. 329
130609ｨｰ0208ｨ 04ｨｪ04ｨｦ ｨｺ06ｨｬ070502ｨｺ09 09 ｨｨ09090502010609ｨ ｨｪｨｪ0401 0902ｨｬ02ｨｪｨ 01 070302ｨｪｨｨ0204 ｨ 0405 0708020109ｨｰｨ 090502ｨｪ 10 000608ｨｬｨ ｨｬｨｨ (08ｨｨ09.). 07 090608ｨｰ0609 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ, 09ｨｨ04ｨ ｨｨ 000208ｨ 09040709ｨｨ05ｨｨ 06ｨｰ 9 0106 10 ｨｨ0406000608ｨｬ 09 08ｨ 04ｨｪ06ｨｦ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺ06ｨｦ 07060109ｨｨ03ｨｪ0609ｨｰｨｹ06. 050608ｨｰｨ 0800ｨ ｨｰｨ ｨｨ 09ｨｨ04ｨ ｨｨｨｬ0205ｨｨ 09010603ｨｨｨｦ ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ04ｨｦ 07080600ｨｨ05ｨｹ, ｨｪ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2 ｨｮ 090608ｨｰｨ 0800ｨ ｨｰｨ 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ ｨ 06050202 090408ｨ 0302ｨｪｨ. 060502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺｨｨ02 07080600ｨｨ05ｨｨ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 ｨｮ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 090608ｨｰ0609 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ ｨｬ0700ｨｺ06ｨｦ 070302ｨｪｨｨ0204 Triticum aestivum L. ｨｨ 0802ｨｺ06ｨｬｨ ｨｨｨｪｨ ｨｪｨｰｨｪ0401 ｨｨｨｪｨ 080201ｨｪ0401 05ｨｨｨｪｨｨｨｦ ｨｺｨ 08ｨｰｨｨ08ｨｮ06ｨｴ02ｨｦ 070607ｨｮ050702ｨｨｨｨ ITMI. 0106ｨｬ0208ｨ ｨｬ 05ｨｨｨｪｨｨｨｦ ITMI 090606ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｰ 02ｨｨ000804 ｨｪｨ 01 0106080603ｨｺｨ ｨｬｨｨ; 02ｨｨ000804 090708ｨ 09ｨ ｪ ｨｬｨ 08ｨｺ020804 ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ 090904, ｨｺ02ｨ ; 09010605ｨｹ 010608060302ｨｺ 070806ｨｪｨｮｨｬ02080609ｨ ｨｪ04 ｨｨ0406000608ｨｬ04 0509ｨｰ0208ｨ 04. 07 08080691 ｨ 040506 06ｨ ｨｪｨ 08ｨｮ0302ｨｪ06 7, 09 080806177 ｪ 8, ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI ｪ 06ｨｰ 7 0106 10 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04. 060406000208ｨｬ02ｨｪｨｰｨｪ0402 07080600ｨｨ05ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0108ｨｮ00 06ｨｰ 0108ｨｮ00ｨ. 060605ｨｹｨｺ06 07080600ｨｨ05ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI10 ｨｨ ITMI29 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ 09010603ｨｨ ｨｺｨ ｨｺ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ, ｨｰｨ ｨｺ ｨｨ 0706 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ 090609ｨｰｨ 09ｨｮ, 0601ｨｪｨ ｨｺ06 06ｨｪｨｨ 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 06ｨｰ 07080600ｨｨ0502ｨｦ 05ｨｨｨｪｨｨｨｦ 08080691 ｨｨ 080806177, ｨ ｨｰｨ ｨｺ0302 06ｨｰ 07080600ｨｨ0502ｨｦ ｨｮ 09090201 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 090608ｨｰ0609, ﾂｨｰ06 0706040906050702ｨｰ ｨｨ09070605ｨｹ040609ｨ ｨｰｨｹ ｨｨ01 010507 ｨ ｨｪｨ 05ｨｨ04ｨ 0708ｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ0701 09 0506ｨ 04ｨｬ ｨｨ04 ｨｨ09090502010609ｨ ｨｪｨｪ0401 090608ｨｰ0609 ｨｨ05ｨｨ 09 05ｨｨｨｪｨｨ07ｨｬｨｨ 08080691 ｨｨ05ｨｨ 080806177. 020507 09090201 06ｨ 08ｨ 04020609 ｨ 040506 01ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 Est-8, Est-9 ｨｨ Est-10. 0102ｨｰ0208060002ｨｪｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｰ0605ｨｹｨｺ06 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ ｨｨ ｨｺ0605ｨｨ- ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ 090609ｨｰｨ 09ｨｮ 0509ｨｰ0208ｨ 04 09 ｨ 0605ｨｹ0302ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ 090906ｨｦ ｪ Est-1, Est- 330
132, Est-3, Est-4, Est-5, Est-6 ｨｨ Est-7. 0609ｨｰ0208ｨ 0404 Est-2 ｨｨ Est-4 0708ｨｨ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨｨ ｨｮ 09090201 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 06ｨ 08ｨ 04020609, 0601ｨｪｨ ｨｺ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 09040302 ｨｮ 090608ｨｰ0609 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ, 0800ｨ ｨｰｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI7, ITMI83, ITMI115, ｨ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-4 ｪ ｨｮ 090608ｨｰ0609 0505ｨ ｨｰｨ, 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI10, ITMI29, ITMI32, ITMI44, ITMI47, ITMI88, ITMI89. 060406000608ｨｬｨ Est-6 ｨ 0405ｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨ ｨｮ 09090201 060202ｨｪ02ｨｪｨｪ0401 090608ｨｰ0609 ｨｨ 09090201 05ｨｨｨｪｨｨｨｦ ITMI, 04ｨ ｨｨ09ｨｺ0506 ﾂ02ｨｪｨｨ02ｨｬ 05ｨｨｨｪｨｨｨｦ 08080691 ｨｨ 080806177. 00ｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-6 09040709ｨｨ05ｨｨ ｨｮ 090608ｨｰｨ 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ ITMI89, ｨｮ 0609ｨｰｨ 05ｨｹｨｪ0401 090608ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI 06ｨｪｨ 09ｨ 08ｨｹｨｨ080609ｨ 05ｨ 06ｨｰ ｨｪｨｨ04ｨｺ06ｨｦ 0106 09080201ｨｪ02ｨｦ. 060406000608ｨｬｨ Est-1 0708ｨｨ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨ ｨｮ 09090201 06ｨ 08ｨ 04020609, 04ｨ ｨｨ09ｨｺ0506 ﾂ02ｨｪｨｨ02ｨｬ 090608ｨｰｨ 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｦ ITMI47, ITMI88 ｨｨ ITMI89. 060406000608ｨｬｨ Est-7 ｨ 0405ｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨ ｨｮ 090608ｨｰ0609 0505ｨ ｨｰｨ, 0906ｨ ｨ 09ｨ ｨｨ 000208ｨ, ｨ ｨｰｨ ｨｺ0302 ｨｮ 05ｨｨｨｪｨｨｨｦ ITMI10, ITMI29, ITMI44, ITMI47, ITMI57, ITMI83 ｨｨ ITMI89. 01ｨ 05ｨｨ ﾂｨｨ02 ｨｨ0406000608ｨｬ04 Est-3 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｮ 09090201 06ｨ 08ｨ 04020609, ｨｺ0806ｨｬ02 090608ｨｰｨ 0906ｨ ｨ 09ｨ, ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-3 ｨ 0405ｨ ｨｪｨ ｨｨｨ 0605ｨｹ0302ｨｦ ｨｮ 090608ｨｰｨ 000208ｨ. 0609ｨｰ0208ｨ 04ｨ Est-5 06ｨｰ09ｨｮｨｰ09ｨｰ090609ｨ 05ｨ ｨｰ0605ｨｹｨｺ06 ｨｮ 05ｨｨｨｪｨｨｨｦ 08080691, ITMI29, ITMI83 ｨｨ ITMI115, 0202 ｨｬｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪｨｮ06 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ 06ｨｰｨｬ02 ﾂｨ 05ｨｨ ｨｮ 090608ｨｰ0609 0505ｨ ｨｰｨ, 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ ITMI89. 06ｨｪｨｰ02080209ｨｪ06, ﾂｨｰ06 090608ｨｰ 000208ｨ, 0201ｨｨｨｪ09ｨｰ0902ｨｪｨｪ04ｨｦ 0604ｨｨｨｬ04ｨｦ 09080201ｨｨ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 06ｨ 08ｨ 04020609, 06ｨ 05ｨ 01ｨ 05 01020907ｨｰｨｹ06 ｨｨ04 01020907ｨｰｨｨ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04. 0308ｨｨ 05ｨｰ06ｨｬ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨｨ0406000608ｨｬ 0906 09080201ｨｪ02ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ 090906ｨｦ ｨｮ ｨｪ020006 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 09040302, ﾂ02ｨｬ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ ｨｨ0406000608ｨｬ 09 ｨｪｨｨ04ｨｺ06ｨｦ ｨｨ 09040906ｨｺ06ｨｦ ｨｬ060502ｨｺｨｮ050708ｨｪ06ｨｦ ｨｬｨ 090906ｨｦ. 0606 0209ｨｰｨｹ 090608ｨｰ 000208ｨ ｨｬ0603ｨｪ06 ｨｨ09070605ｨｹ040609ｨ ｨｰｨｹ 09 ｨｺｨ ﾂ0209ｨｰ0902 080601ｨｨｨｰ0205ｨｹ09ｨｺ06ｨｦ 000608ｨｬ04 0708ｨｨ 07080609020102ｨｪｨｨｨｨ 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ07 09 0506ｨ 04ｨｬ ｨｨ04 060202ｨｪ02ｨｪｨｪ0401 0706 ｨｨ0406000608ｨｬ02ｨｪｨｰｨｪ06ｨｬｨｮ 090702ｨｺｨｰ08ｨｮ 0509ｨｰ0208ｨ 04 06ｨ 08ｨ 04020609. 09ｨｨｨｪｨｨｨｨ 08080691 ｨｨ 080806177 ｨｰｨ ｨｺ0302 ｨｬ0600ｨｮｨｰ 0905ｨｮ03ｨｨｨｰｨｹ 080601ｨｨｨｰ0205ｨｹ09ｨｺｨｨｨｬｨｨ 000608ｨｬｨ ｨｬｨｨ 09 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨ0701 0108ｨｮ00 09 0108ｨｮ0006ｨｬ ｨｨ05ｨｨ 09 0609ｨｰｨ 05ｨｹｨｪ04ｨｬｨｨ ｨｨ09090502010609ｨ ｨｪｨｪ04ｨｬｨｨ 05ｨｨｨｪｨｨ07ｨｬｨｨ ｨｨ 090608ｨｰｨ ｨｬｨｨ. 050608ｨｰｨ 0505ｨ ｨｰｨ ｨｨ 0906ｨ ｨ 09ｨ 06ｨ 05ｨ 01ｨ 05ｨｨ ｨｮｨｪｨｨｨｺｨ 05ｨｹｨｪ04ｨｬ 050502ｨｺｨｰ080600060802ｨｰｨｨ ﾂ0209ｨｺｨｨｨｬ 090702ｨｺｨｰ0806ｨｬ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04. 050608ｨｰ 0505ｨ ｨｰｨ 06ｨｰ05ｨｨ ﾂｨ 02ｨｰ0907 06ｨｰ 090608ｨｰｨ 0906ｨ ｨ 09ｨ ｨｪｨ 05ｨｨ- ﾂｨｨ02ｨｬ ｨｨ0406000608ｨｬ04 Est-3 ｨｨ ｨ 06050202 09040906ｨｺ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 Est-4. 050608ｨｰｨ 0800ｨ ｨｰｨ ｨｨ 09ｨｨ04ｨ 0106ｨｰｨｹ ｨｨ 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ040609ｨ 05ｨｨ09ｨｹ 09010603ｨｨｨｬｨｨ 090702ｨｺｨｰ08ｨ ｨｬｨｨ, ｨｪ06 ｨｮ 090608ｨｰｨ 0800ｨ ｨｰｨ ｨ 0405ｨ 06ｨｰｨｬ02 ﾂ02ｨｪｨ ｨ 06050202 09040906ｨｺｨ 07 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｹ Est-2. 09ｨｨｨｪｨｨｨｨ ITMI 06ｨ 05ｨ 01ｨ 05ｨｨ 09010603ｨｨｨｬｨｨ 07080600ｨｨ0507ｨｬｨｨ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04, 0601ｨｪｨ ｨｺ06 06ｨｰ05ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0108ｨｮ00 06ｨｰ 0108ｨｮ00ｨ ｨｨ 06ｨｰ 05ｨｨｨｪｨｨｨｦ 08080691 ｨｨ 080806177, ｨ ｨｰｨ ｨｺ0302 06ｨｰ 09090201 090608ｨｰ0609. 09ｨｨｨｪｨｨｨｨ ITMI7, ITMI83, ITMI89 ｨｨ ITMI115 ｨｨｨｬ0205ｨｨ 0709ｨｪ06 090408ｨ 0302ｨｪｨｪ0402 06ｨｰ05ｨｨ ﾂｨｨｨｰ0205ｨｹｨｪ0402 ｨｬ06080006050600ｨｨ ﾂ0209ｨｺｨｨ02 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨｨ (30), ﾂｨｰ06 0706040906050702ｨｰ ｨｨ09070605ｨｹ040609ｨ ｨｰｨｹ ｨｨ01 09 ｨｺｨ ﾂ0209ｨｰ0902 ｨｨ09010601ｨｪ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ 0708ｨｨ 07080609020102ｨｪｨｨｨｨ 0002ｨｪ02ｨｰｨｨｨｺ06-09020502ｨｺ02ｨｨ06ｨｪｨｪ0401 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨｨｦ. 08ｨ 030104ｨｦ ｨｨ04 18 090608ｨｰ0609 ｨｨ 05ｨｨｨｪｨｨｨｦ 06ｨ 05ｨ 01ｨ 05 06ｨｰ05ｨｨ ﾂｨｪ04ｨｬ 06ｨｰ 0108ｨｮ00ｨｨ01 06ｨ 08ｨ 04020609 0002ｨｪ06ｨｰｨｨ0706ｨｬ. 0004 060202ｨｪｨｨ05ｨｨ ｨｨ01 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ 0706 10 090407090502ｨｪｨｪ04ｨｬ 0506ｨｺｨｮ09ｨ ｨｬ, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨｨｬ ｨｨ0406000608ｨｬ04 0509ｨｰ0208ｨ 04. 05080201ｨｪ0707 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ (H) 090609ｨｰｨ 09ｨｨ05ｨ 0,924; 01ｨｨ0907020809ｨｨ07 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ 010507 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ060006 ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ 04020609 Var(H) = 0,0004. 030609ｨｺ0605ｨｹｨｺｨｮ 08ｨ 04ｨｬ0208 0904ｨ 0608ｨｺｨｨ ｨ 0405 06ｨｰｨｪ0609ｨｨｨｰ0205ｨｹｨｪ06 ｨｬｨ 05 ｨｨ 070605ｨｨｨｬ060800ｨｪ04ｨｬｨｨ 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｰ0605ｨｹｨｺ06 5 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04, 010507 08ｨ 09 ﾂ02ｨｰｨ 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｬ04 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 070601010601, 0708020105060302ｨｪｨｪ04ｨｦ 00. Nei (25) ｨｨ 09. 0002ｨｦ08 (26), ｨｺ06ｨｰ060804ｨｦ 070604090605ｨｨ05 ｨ 06050202 ｨｰ06 ﾂｨｪ06 060202ｨｪｨｨｨｰｨｹ 09080201ｨｪ0606 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｨ 0202 01ｨｨ0907020809ｨｨ06. 000407090502ｨｪｨｪｨ 07 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ 05ｨ 09ｨｹ ｨｺｨ ｨｺ 09080201ｨｪ0707 07060802ｨｨ07 0506ｨｺｨｮ090609 09 0109ｨｮｨｬ07 08ｨ 0405ｨｨ ﾂｨｪ04ｨｬｨｨ ｨ 0505020507ｨｬｨｨ 09 0601ｨｪ06ｨｬ 0506ｨｺｨｮ0902 ｨｮ 0601ｨｪ06ｨｦ 060906ｨ ｨｨ ｨｨ ｨｬ060005ｨ ｨ 04ｨｰｨｹ 0607080201020502ｨｪｨ ｨｺｨ ｨｺ ｨｪｨ ｨ 050601ｨ 02ｨｬｨ 07 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ, 01ｨ 08ｨ ｨｺｨｰ0208ｨｨ04ｨｮ06ｨｴｨ 07 ﾂｨ 09ｨｰｨｹ 0002ｨｪ0609, 0706 ｨｺ06ｨｰ060804ｨｬ 070607ｨｮ050702ｨｨ07 0002ｨｰ02080604ｨｨ0006ｨｰｨｪｨ (27). 0209ｨｪ0609ｨｪ0602 06ｨｰ05ｨｨ ﾂｨｨ02 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ01 0706010106010609 04ｨ ｨｺ0506 ﾂｨ 02ｨｰ0907 09 ｨｰ06ｨｬ, ﾂｨｰ06 ｨ 09ｨｰ060804 (25, 26) 0904090205ｨｨ 0201ｨｨｨｪ0606ｨ 08ｨ 04ｨｪ06 08ｨ 090708020102050702ｨｬ0602 ｨｬｨｨｨｪｨｨｨｬｨ 05ｨｹｨｪ0602 06ｨｰｨｺ0506ｨｪ02ｨｪｨｨ02 ｨｪ0209ｨｬ02ｨｴ02ｨｪｨｪ06ｨｦ 060202ｨｪｨｺｨｨ 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ (01) 09 090606ｨｰ0902ｨｰ09ｨｰ09ｨｨｨｨ 09 020006 ｨｰ06 ﾂｨｪ04ｨｬ 04ｨｪｨ ﾂ02ｨｪｨｨ02ｨｬ 01ｨｨ0907020809ｨｨｨｨ Var(H). 020507 05ｨｰ060006 06ｨｪｨｨ 070605ｨｮ ﾂｨｨ05ｨｨ 000608ｨｬｨｮ0504, 0706040906050706ｨｴｨｨ02 08ｨ 0909 ﾂｨｨｨｰｨ ｨｰｨｹ 0506ｨ 06ｨｦ ｨｬｨｪ060006 ﾂ0502ｨｪ 09 ｨｪｨ ｨ 060802 07020802ｨｬ02ｨｪｨｪ0401, 08ｨ 0907080201020502ｨｪｨｪ0401 ｨｬｨｮ05ｨｹｨｰｨｨｨｪ06-331
13ｨｬｨｨｨ 05ｨｹｨｪ06, ﾂｨｰ06 01ｨ 02ｨｰ 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ 09040709050702ｨｬｨｮ06 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｹ ｨｺｨ ｨｺ ｨｬ0208ｨｮ ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ060006 070605ｨｨｨｬ060800ｨｨ04ｨｬｨ, 0905ｨｮ03ｨ ｨｴ020006 09ｨ 03ｨｪ04ｨｬ ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰ06ｨｬ 0708ｨｨ 090609ｨｰｨ 090502ｨｪｨｨｨｨ 0705ｨ ｨｪ0609 09020502ｨｺ02ｨｨ06ｨｪｨｪ0401 0708060008ｨ ｨｬｨｬ ｨｨ 0601ｨｪｨｨｨｬ ｨｨ04 ｨｺ0506 ﾂ02090401 ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ06-09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ01 0706ｨｺｨ 04ｨ ｨｰ020502ｨｦ 0708ｨｨ ｨｨ01 0904070605ｨｪ02ｨｪｨｨｨｨ. 0201ｨｪｨ ｨｺ06 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｨ02 0506ｨ 0401 09020502ｨｺ02ｨｨ06ｨｪｨｪ06 04ｨｪｨ ﾂｨｨｨｬ0401 090906ｨｦ09ｨｰ09 04ｨ 09ｨｨ09ｨｨｨｰ 06ｨｰ 0002ｨｪ06ｨｰｨｨ07ｨ 06ｨ 08ｨ 04020609, ｨ 090502010609ｨ ｨｰ0205ｨｹｨｪ06, ｨｨｨｪ000608ｨｬｨ 02ｨｨ06ｨｪｨｪ04ｨｦ 070605ｨｨｨｬ060800ｨｨ04ｨｬ 0209ｨｰｨｹ ｨｪ02 ﾂｨｰ06 ｨｨｨｪ0602, ｨｺｨ ｨｺ 06ｨｰ08ｨ 0302ｨｪｨｨ02 09040709050702ｨｬ06ｨｦ ｨｪｨ 0002ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺ06ｨｬ ｨｨ(ｨｨ05ｨｨ) ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺ06ｨｬ ｨｮ080609ｨｪ02 ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｨ 08ｨ 0907080201020502ｨｪｨｨ07 09 0002ｨｪ06ｨｬ02 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 0102ｨｰ0208ｨｬｨｨｨｪｨ ｨｪｨｰ (0002ｨｪ0609, 0506ｨｺｨｮ090609 010806ｨｬ060906ｨｬ), 060708020102050706ｨｴｨｨ01 07080607090502ｨｪｨｨ02 ｨｨ04ｨｮ ﾂｨ 02ｨｬ0401 0708ｨｨ04ｨｪｨ ｨｺ0609. 04ｨ ｨｪ0202 ｨ 040506 0706ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 0002ｨｪ04, ｨｺ0601ｨｨ08ｨｮ06ｨｴｨｨ02 ｨｨ0406000608ｨｬ04 0509ｨｰ0208ｨ 04, 08ｨ 09070605ｨ 00ｨ 06ｨｰ0907 09 010806ｨｬ060906ｨｬ02 3 09090201 ｨｰ080201 0002ｨｪ06ｨｬ0609 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ 070302ｨｪｨｨ0204 (20-22). 0604 05ｨｰ060006 09050201ｨｮ02ｨｰ, ﾂｨｰ06 09 0708060202090902 050906050602ｨｨｨｨ 070806ｨｨ09010601ｨｨ05ｨ 01ｨｨ0000020802ｨｪ02ｨｨｨ 02ｨｨ07 05ｨｰ06ｨｦ ﾂｨ 09ｨｰｨｨ 0002ｨｪ06ｨｬ0609. 000603ｨｪ06 070802010706050603ｨｨｨｰｨｹ ｨｺｨ ｨｺ ｨｬｨｨｨｪｨｨｨｬｨｮｨｬ 0109ｨ ｨｬｨｮｨｰｨ 02ｨｨ06ｨｪｨｪ0401 0906ｨ 04ｨｰｨｨ07, 07060905ｨｨ070903ｨｨ01 ｨｪｨ 08ｨ 0907080201020502ｨｪｨｨ02 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 09080201ｨｨ 0006ｨｬ06050600ｨｨ ﾂｨｪ0401 010806ｨｬ060906ｨｬ. 06ｨｰ06 ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ ｨｰｨ ｨｺ0302 06ｨｰｨｪ020902ｨｪ06 ｨｺ 00ｨｨｨ 08ｨｨ01ｨｪ04ｨｬ 000608ｨｬｨ ｨｬ ｨｨ0406000208ｨｬ02ｨｪｨｰ0609 (01ｨｨｨｬ020804, ｨｰ08ｨｨｨｬ020804 ｨｨ05ｨｨ ｨｰ02ｨｰ08ｨ ｨｬ020804 ｨｨ ｨｰ.07.), 070609ｨｺ0605ｨｹｨｺｨｮ 070605ｨｮ ﾂ02ｨｪｨｨ02 ｨｰｨ ｨｺｨｨ01 000608ｨｬ 06ｨ ｨｮ090506090502ｨｪ06 ｨｰ0605ｨｹｨｺ06 ｨｪｨ 05ｨｨ ﾂｨｨ02ｨｬ 08ｨ 0405ｨｨ ﾂｨｪ0401 09ｨ 08ｨｨｨ ｨｪｨｰ0609 070605ｨｨｨｬ02080609 09 ｨｰｨｺｨ ｨｪ0701 ｨｨ 060800ｨ ｨｪｨ 01 08ｨ 09ｨｰ02ｨｪｨｨｨｦ (15, 18, 19). 0606ｨｰ 00ｨ ｨｺｨｰ, ﾂｨｰ06 090407090502ｨｪｨｪ0402 ｨｨ0406000208ｨｬ02ｨｪｨｰ04 ｨｺ0601ｨｨ08ｨｮ06ｨｰ0907 0006ｨｬ0206050600ｨｨ ﾂｨｪ04ｨｬｨｨ 010806ｨｬ060906ｨｬｨ ｨｬｨｨ (ｨｨ, 090604ｨｬ0603ｨｪ06, ｨｨ01 070502 ﾂｨ ｨｬｨｨ), 010608060306 09060005ｨ 09ｨｮ02ｨｰ0907 09 00ｨｨ0706ｨｰ020406ｨｦ 06 0908ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ06 ｨｪ0201ｨ 09ｨｪ02ｨｦ 01ｨｨ0000020802ｨｪ02ｨｨｨ 02ｨｨｨｨ 0002ｨｪ06ｨｬ0609 08, 00 ｨｨ D ｨｮ 070302ｨｪｨｨ0204 (21). 03020802010601 06ｨｰ ｨｰ02ｨｰ08ｨ 070506ｨｨ01ｨｪ0609ｨｰｨｨ ｨｺ 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ04ｨｬ 000608ｨｬｨ ｨｬ, 070601ｨｰ0902080301ｨ 02ｨｬ04ｨｦ ｨ 0101ｨｨｨｰｨｨ09ｨｪ0609ｨｰｨｹ06 ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04, 0708ｨｨ090601ｨｨｨｰ ｨｺ 06ｨ 06ｨ ｨｴｨ 06ｨｴ02ｨｬｨｮ 04ｨ ｨｺ0506 ﾂ02ｨｪｨｨ06 06 090604ｨｬ0603ｨｪ0609ｨｰｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｰｨｹ 09ｨｨ09ｨｰ02ｨｬｨｮ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04 09 060800ｨ ｨｪｨｨ04ｨ 02ｨｨｨｨ ｨｨ ｨｮ090205ｨｨ- ﾂ02ｨｪｨｨｨｨ 0705ｨ 09ｨｰｨｨ ﾂｨｪ0609ｨｰｨｨ 070605ｨｨ070506ｨｨ010609 ｨｨ ｨｨ01 ｨ 01ｨ 07ｨｰｨ 02ｨｨｨｨ ｨｺ 08ｨ 0405ｨｨ ﾂｨｪ04ｨｬ ｨｮ09050609ｨｨ07ｨｬ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ 0908020104. 06ｨｰ06 09060005ｨ 09ｨｮ02ｨｰ0907 09 ｨｪｨ ｨ 05060102ｨｪｨｨ02ｨｬ, ﾂｨｰ06 070605ｨｨ070506ｨｨ0104 0907060906ｨ ｨｪ04 ｨ 06050202 05000002ｨｺｨｰｨｨ09ｨｪ06 ｨｺ060506ｨｪｨｨ04ｨｨ080609ｨ ｨｰｨｹ ｨ 0802ｨ 0504 0706 0908ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨ 05ｨｨ04ｨｺ0608060109ｨｰ0902ｨｪｨｪ04ｨｬｨｨ 01ｨｨ070506ｨｨ01ｨｪ04ｨｬｨｨ 09ｨｨ01ｨ ｨｬｨｨ (3, 6). 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, 070605ｨｮ ﾂ02ｨｪｨｪ0402 080204ｨｮ05ｨｹｨｰｨ ｨｰ04 070604090605ｨｨ05ｨｨ 0904010205ｨｨｨｰｨｹ ｨｨ04 ｨｨ09090502010609ｨ ｨｪｨｪ060006 ｨｬｨ ｨｰ0208ｨｨｨ 05ｨ ｨｪｨ ｨｨｨ 06050202 070208090702ｨｺｨｰｨｨ09ｨｪ0402 09020502ｨｺ02ｨｨ06ｨｪｨｪ0402 080601ｨｨｨｰ0205ｨｹ09ｨｺｨｨ02 000608ｨｬ04 ｪ 090608ｨｰｨ 0505ｨ ｨｰｨ, 000208ｨ ｨｨ 05ｨｨｨｪｨｨｨｨ 08080691, 080806177, ITMI7, ITMI44, ITMI83 ｨｨ ITMI115, ｨｺ06ｨｰ06080402 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ ｨｪ02 ｨｰ0605ｨｹｨｺ06 0706 0002ｨｪ06050600ｨｨ- ﾂ0209ｨｺｨｨｨｬ ｨｨ 0002ｨｪ06ｨｰｨｨ07ｨｨ ﾂ0209ｨｺｨｨｨｬ 0706ｨｺｨ 04ｨ ｨｰ020507ｨｬ, ｨｪ06 ｨｨ 0706 090702ｨｺｨｰ08ｨ ｨｬ ｨｨ0406000608ｨｬ 0509ｨｰ0208ｨ 04. 0308ｨｨ 05ｨｰ06ｨｬ 09ｨｮｨｴ0209ｨｰ09ｨｮ02ｨｰ 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 0607080201020502ｨｪｨｨ07 070605ｨｨｨｬ060800ｨｨ04ｨｬｨ ｨｪ02 ｨｰ0605ｨｹｨｺ06 ｨｮ ｨｨ09010601ｨｪ0401 000608ｨｬ, ｨｪ06 ｨｨ ｨｮ 07080201070605ｨ 00ｨ 02ｨｬ0401 0706ｨｰ06ｨｬｨｺ0609, 070605ｨｮ ﾂ02ｨｪｨｪ0401 09 080204ｨｮ05ｨｹｨｰｨ ｨｰ02 09ｨｺ0802ｨｴｨｨ09ｨ ｨｪｨｨｨｦ. 01ｨ 05ｨｨ ﾂｨｨ02 01020907ｨｰｨｨ ｨｨ0406000608ｨｬ 010205ｨ 02ｨｰ 0509ｨｰ0208ｨ 0404 0002ｨｺ09ｨ 070506ｨｨ01ｨｪ06ｨｦ 070302ｨｪｨｨ0204 ｨｮ0106ｨ ｨｪ04ｨｬ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨｨｬ ｨｬｨ 08ｨｺ020806ｨｬ ｨｨ 0706040906050702ｨｰ 070806090601ｨｨｨｰｨｹ ｨｺｨ ｨｺ 00ｨｨ04ｨｨ0605060006-ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ02, ｨｰｨ ｨｺ ｨｨ 0002ｨｪ02ｨｰｨｨｨｺ06-09020502ｨｺ02ｨｨ06ｨｪｨｪ0402 ｨｨ09090502010609ｨ ｨｪｨｨ07. 0906050202 0102ｨｰｨ 05ｨｹｨｪｨ 07 ｨｨｨｪｨｰ0208070802ｨｰｨ 02ｨｨ07 070605ｨｮ ﾂ02ｨｪｨｪ0401 ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ0401 07080600ｨｨ0502ｨｦ 09ｨｰｨ ｨｪ02ｨｰ 090604ｨｬ0603ｨｪ06ｨｦ 0706090502 0908ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ060006 ｨ ｨｪｨ 05ｨｨ04ｨ ｨｨ0406000208ｨｬ02ｨｪｨｰｨｪ0401 07080600ｨｨ0502ｨｦ ｨｮ 01ｨｨ070506ｨｨ01ｨｪ0401 0106ｨｪ06080609 0002ｨｪ06ｨｬ0609 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 000608ｨｬ. 000408ｨ 03ｨ 02ｨｬ ｨ 05ｨ 000601ｨ 08ｨｪ0609ｨｰｨｹ 01.00. 0806 ﾂ0208ｨｨｨｪ06ｨｦ 04ｨ 0706ｨｬ06ｨｴｨｹ 0708ｨｨ 07080609020102ｨｪｨｨｨｨ 09ｨｰｨ ｨｰｨｨ09ｨｰｨｨ ﾂ0209ｨｺｨｨ01 08ｨ 09 ﾂ02ｨｰ0609 0002ｨｰ02080604ｨｨ0006ｨｰｨｪ0609ｨｰｨｨ ｨｨ 0202 01ｨｨ0907020809ｨｨｨｨ. 1 010609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ04ｨｦ ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰ 00060501060904, 2009 04020907ｨｮｨ 05ｨｨｨｺｨ 000605010609ｨ, 00. 08ｨｨ03ｨｨｨｪ0209, ｨｮ05. 00ｨ ｨｰ020209ｨｨ ﾂ, 60; 2 0801090107 000609ｨｺ060909ｨｺｨｨｨｦ 0106060509 000102ｨｬ ﾂｨｨｨｪ0609ｨｺｨ 03, 143026 04060909ｨｨ07, 000609ｨｺ060909ｨｺｨ 07 06ｨ 05., 0201ｨｨｨｪ02060909ｨｺｨｨｨｦ 08-ｨｪ, 0403 01060906ｨｨ09ｨ ｨｪ060909ｨｺ0602, ｨｮ05. 08ｨ 05ｨｨｨｪｨｨｨｪｨ, 1; 3 0801090107 0800080600ｨｨ04ｨｨ ﾂ0209ｨｺｨｨｨｦ 010606, 195220 04060909ｨｨ07, 00. 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800, 0108ｨ 0301ｨ ｨｪ09ｨｺｨｨｨｦ 0708060907., 14, 4 0801090107 080600 00090208060909ｨｨｨｦ09ｨｺｨｨｨｦ ｨｨｨｪ09ｨｰｨｨｨｰｨｮｨｰ 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 080209ｨｮ08090609 08ｨ 09ｨｰ02ｨｪｨｨｨｦ ｨｨｨｬ. 01.06. 00ｨ 09ｨｨ050609ｨ (000604), 190000 04060909ｨｨ07, 00. 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800, ｨｮ05. 090605ｨｹ03ｨ 07 00060809ｨｺｨ 07, 42-44, e-mail: yu.chesnokov@vir.nw.ru 030609ｨｰｨｮ07ｨｨ05ｨ 09 080201ｨ ｨｺ02ｨｨ06 25 ｨｬｨ 08ｨｰｨ 2016 000601ｨ 332
13Sel ｯskokhozyaistvennaya biologiya [Agricultural Biology], 2016, V. 51, 01 3, pp. 327-334 ISOZYMIC ANALYSIS OF ESTERASES IN MATURE SEEDS OF HEXAPLOID SOFT WHEAT (Triticum aestivum L.) A.S. Rudakova 1, S.V. Rudakov 1, N.V. Davydova 2, G.V. Mirskaya 3, E.V. Zhuravleva 2, Yu.V. Chesnokov 4 1 State University of Moldova, 60, str. Mateevich, Kishinev, 2009 Moldova; 2 00Nemchinovka03 Moscow Research Institute for Agriculture, Federal Agency of Scientific Organizations, 1, ul. Kalinina, RP Novoivanovskoe, Odintsovo Region, Moscow Province, 143026 Russia; 3 Agrophysical Research Institute, Federal Agency of Scientific Organizations, 14, Grazhdanskii prosp., St. Petersburg, 195220 Russia; 4 Federal Research Center the N.I. Vavilov All-Russian Institute of Plant Genetic Resources, Federal Agency of Scientific Organizations, 42-44, ul. Bol ｯshaya Morskaya, St. Petersburg, 190000 Russia, e-mail yu.chesnokov@vir.nw.ru Acknowledgements: We are grateful to Dr. N.V. Kocherina for help with the statistical calculations of heterozygosity and its dispersion. Received March 25, 2016 doi: 10.15389/agrobiology.2016.3.327eng Abstract Esterases represent a large group of enzymes that catalyze cleavage of multiple-ester bonds. In general, they are divided into four types: cholinesterases (most frequently, these are identified using ordinary electrophoretic analysis), acetylesterases, arylesterases and carboxyl esterases. Plant carboxyl esterases catalyze conversion of the esters into bioactive acids and alcohols, thereby playing a key role in many biological processes. Lack of epistatic interactions as well as a co-dominant nature of the inheritance of the esterase isozymes makes them meaningful for quick and accessible investigation of the processes of biochemical adaptation to environmental changes. Such a type of markers, which is convenient for solution of practical problems of selection, can be used as a tool that can speed up and simplify the selection of the significant material. The aim of this study was to estimate the isoenzyme profile of esterases isolated from mature seeds, and to ascertain, using such a biochemical marker, the polymorphism among samples of promising breeding material of the hexaploid wheat (Triticum aestivum L.). Ripe seeds from following wheat cultivars were used as samples: Zlata, Lyubava, Agatha, Lisa (spring wheat) and Mera (winter wheat) (originated by Moscow Agricultural Research Institute 00Nemchinovka03, Moscow Province); lines AFI91 and AFI177 (spring wheat) and recombinant inbred lines of the mapping populations ITMI ｪ 7, 10, 29, 32, 44, 47, 57, 83, 88, 89, and 115 (spring wheat) (originated by Agrophysical Research Institute ｪ AFI, St. Petersburg). The seeds were ground in a porcelain mortar and the flour was sieved. The enzymes were extracted from the flour and subjected to vertical native electrophoresis using 4 % concentrating and 8 % separating polyacrylamide gels. Molecular weight markers were Page Ruler Prestained Protein Ladder 00Thermo Scientific03 (Lithuania). After electrophoresis, gels were treated with a reagent for a nonspecific esterase and scanned. Individual electrophoretic profile of each sample was estimated. Heterozygosity and its dispersion were calculated. The esterase complex in the wheat seeds studied was represented by 10 isoforms. From 9 to 10 isoforms of various electrophoretic mobility have been identified in cultivars Zlata, Lyubava, Agatha, Lisa and Mera, 7 isoforms were found in line AFI91, 8 isoforms ｪ in AFI177, and from 7 to 10 isoforms ｪ in ITMI lines. All samples were characterized by the presence of esterases isoforms Est-8, Est-9 and Est-10. Heterogeneity was only found in the qualitative and quantitative composition of esterases with a greater molecular weight ｪ Est-1, Est-2, Est-3, Est-4, Est-5, Est-6, and Est-7. Each variety among eighteen varieties and lines had a genotype different from that in the other samples. The average heterozygosity (H) of samples within 10 loci encoding esterase isoforms was 0.924; the dispersion of heterozygosity for all the samples studied was Var(H) = 0.0004. As a result of the analyses, following most promising breeding parental forms are varieties Zlata and Mera, and lines AFI91, AFI177, ITMI7, ITMI44, ITMI83, and ITMI115. Because of the existence of ten isoforms in hexaploid wheat, the esterases might represent a convenient biochemical marker suitable for examination of samples of the hexaploid wheat at physiological, biochemical, and genetic levels. Keywords: hexaploid soft wheat, mature seeds, isozymic analysis, esterase REFERENCES 1. Bou lte r D., Thur ma nn D.A., Tu r ne r B.L. The use of disc electrophoresis of plant proteins in systematics. Taxon, 1966, 15: 135-142 (doi: 10.2307/1217532). 2. S h a w C.R. Isozymes: classification, frequency, and significance. Int. Rev. Cytol., 1969, 25: 297-332 (doi: 10.1016/S0074-7696(08)60206-5). 3. H a r t G.E. Genetics and evolution of multilocus isozymes in hexaploid wheat. Isozymes Curr. Top. Biol. Med. Res., 1983, 10: 365-380. 333
134. B r o w n A.H., C l e g g M.T. Isozyme assessment of plant genetic resources. Isozymes Curr. Top. Biol. Med. Res., 1983, 11: 285-295. 5. N e v o E. Plant genetic resources: prediction by isozyme markers and ecology. Isozymes Curr. Top. Biol. Med. Res., 1987, 16: 247-267. 6. C u m m i n s I., B u r n e t M., E d w a r d s R. Biochemical characterization of esterases active in hydrolysing xenobiotics in wheat and competing weeds. Physilologia Plantarum, 2001, 113: 477-485. 7. C a r v a l h o V.M., M a r q u e s R.M., L a p e n t a A.S., M a c h a d o M.F.P.S. Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae). Genet. Mol. Biol., 2003, 26(2): 195-198 (doi: 10.1590/S1415-47572003000200013). 8. H o u C.-J., H e K., Y a n g L.-M., H u o D.-Q., Y a n g M., H u a n g S., Z h a n g L., S h e n C.-H. Catalytic characteristics of plant-esterase from wheat flour. World J. Microbiol. Biotechnol., 2012, 28: 541-548 (doi: 10.1007/s11274-011-0845-9). 9. Rejon J.D., Zienkiewicz A., Rodrｨｪ guez-garcｨｪ a M.I., Castro A.J. Profiling and functional classification of esterases in olive (Olea europaea) pollen during germination. Ann. Bot., 2012, 110(5): 1035-1045 (doi: 10.1093/aob/mcs174). 10. G e r s h a t e r M.C., C u m m i n s I., E d w a r d s R. Role of a carboxylesterase in herbicide bioactivation in Arabidopsis thaliana. J. Biol. Chem., 2007, 282: 21460-21466 (doi: 10.1074/jbc.M701985200). 11. C u m m i n s I., E d w a r d s R. Purification and cloning of an esterase from the weed blackgrass (Alopecurus myosuroides), which bioactivates aryloxyphenoxypropionate herbicides. Plant J., 2004, 39: 894-904 (doi: 10.1111/j.1365-313X.2004.02174.x). 12. Stuhlfelder C., Mueller M.J., Warzecha H. Cloning and expression of a tomato cdna encoding a methyl jasmonate cleaving esterase. Eur. J. Biochem., 2004, 271: 2976-2983 (doi: 10.1111/j.1432-1033.2004.04227.x). 13. D o g r u E., W a r z e c h a H., S e i b e l F., H a e b e l S., L o t t s p e i c h F., S t o c k i g t J. The gene encoding polyneuridine aldehyde esterase of monoterpenoid indole alkaloid biosynthesis in plants is an ortholog of the alpha/betahydrolase super family. Eur. J. Biochem., 2000, 267: 1397-1406 (doi: 10.1046/j.1432-1327.2000.01136.x). 14. F e n g P.C.C., R u f f T.G., K o s i n s k i W.G. Metabolic deactivation of the herbicide thiazopyr by animal liver esterases. Xenobiotica, 1995, 25: 27-35 (doi: 10.3109/00498259509061830). 15. B a r b e r H.N., D r i s c o l l C.J., V i c k e r y R.S. Enzymic markers for wheat and rye chromosomes. In: Proc. 8 th Int. Wheat Genet. Symp. Aust. Acad. Sci., Canberra, 1968: 116-122. 16. B a r b e r H.N., D r i s c o l l C.J., L o n g P.M., V i c k e r y R.S. Gene similarity of the Triticinae and the study of segmental interchanges. Nature, 1969, 22: 897-898 (doi: 10.1038/222897a0). 17. Bergman J.W. Chromosome locations of genes controlling esterase and malate dehydrogenase isozymes in Triticum. PhD Diss. North Dakota State University, Fargo, 1972. 18. J a a s k a V. Electrophoretic survey of seedling esterased in wheats in relation to their phylogeny. Theor. Appl. Genet., 1980, 56: 273-284 (doi: 10.1007/BF00282570). 19. May C.E., Vickery R.S., Driscoll C.S. Gene control in hexaploid wheat. In: Proc. 4 th Int. Wheat Genet. Symp. Columbia, 1973: 843-849. 20. N a k a i I. Isoenzyme variations in Aegilops and Triticum. 3. Chromosomal basis of the esterase isozyme production in indifferent organs of Chinese Spring wheat. Bot. Mag., 1976, 89: 219-234 (doi: 10.1007/BF02488344). 21. C u b a d d a R., B o z z i n i A., Q u a t r u c c i E. Genetic control of esterases in common wheat. Theor. Appl. Genet., 1975, 45: 290-293 (doi: 10.1007/BF00276681). 22. A i n s w o r t h C.C., G a l e M.D., B a i r d S. The genetic control of grain esterases in hexaploid wheat. Theor. Appl. Genet., 1984, 68: 219-226 (doi: 10.1007/BF00266893). 23. D a v i s B.J. Disc electrophoresis. II. Method and application to human serum proteins. Annals of the NY Academy of Sciences, 1964, 121: 404-427 (doi: 10.1111/j.1749-6632.1964.tb14213.x). 24. M e o n S. Protein, esterase and peroxidase patterns of phytophtora isolates from Cocoa in Malaysia. J. Islamic Acad. Sci., 1988, 1(2): 154-158. 25. N e i M. Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics, 1978, 89: 583-590. 26. Veir B. Analiz geneticheskikh dannykh [Genetic data analysis]. Moscow, 1995: 132-135 (in Russ.). 27. C h e s n o k o v Yu.V., P o c h e p n y a N.V., K o z l e n k o L.V., S i t n i k o v M.N., M i - t r o f a n o v a O.P., S y u k o v V.V., K o c h e t k o v D.V., L o v a s s e r U., B e rner A. Vavilovskii zhurnal genetiki i selektsii, 2012, 16(4/2): 970-998 (in Russ.). 28. C h e s n o k o v Yu.V., G o n c h a r o v a E.A., S i t n i k o v M.N., K o c h e r i n a N.V., Lovasser U., Be rner A. Fiziologiya rastenii, 2014, 61(6): 855-863 (doi: 10.7868/S0015330314060049) (in Russ.). 29. C h e s n o k o v Yu.V., S i t n i k o v M.N., S c h u m l y a n s k a y a N.V., K o c h e r i n a N.V., G o n c h a r o v a E.A., K o z l e n k o L.V., S y u k o v V.V., K o c h e t k o v D.V., L o h w a s s e r U., B 02 rner A. Catalogue of recombinant inbred lines of mapping population ITMI of soft spring wheat Triticum aestivum L. (ecological and geographical trials and QTL mapping). IPK, VIR, St. Petersburg ｪGatersleben, 2014. 30. C h e s n o k o v Yu.V., A r t e m ' e v a A.M. Otsenka mery informatsionnogo polimorfizma geneticheskogo raznoobraziya [Evaluation of the measure of polymorphism information of genetic diversity]. Sel ｯskokhozyaistvennaya Biologiya [Agricultural Biology], 2015, 50(5): 571-578 (doi: 10.15389/agrobiology.2015.5.571eng). 334