130503090505080209020508070506000301010808 0906020902010608, 2013, 01 5, 09. 85-92 00ｨｨｨｺ0806ｨ ｨｨ06050600ｨｨ07 0706 ﾂ0904 070208 579.6/.8:631.46:574.24:575.852'1 doi: 10.15389/agrobiology.2013.5.85rus 0205010200010403 060301020301000606 00 080204000604020008010606 0302 ﾁ00030101020102 00060804020901020102 05020209020305060008 00 0705090200060809 05060800060201080401020102 0302090300020102 0203040608 0302 020801010400 0004050208020304020605000202060603090501020102 0503080003010604020008010608 090609090602060308 01030108 16S-08040108 * 00.08. 020700020008, 03.00. 03030401060108, 08.00. 00030405090808020008, 07.00. 08040701090200, 03.03. 0801020402010200 09ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ02 0706 ﾂ0902ｨｪｨｪ0401 ｨｬｨｨｨｺ0806ｨ ｨｪ0401 090606ｨ ｨｴ0209ｨｰ09 09 ｨｪｨ 09ｨｰ0607ｨｴ0202 090802ｨｬ07 ｨｨ04ｨｮ ﾂｨ 02ｨｰ0907 010609ｨｰｨ ｨｰ06 ﾂｨｪ06 03ｨｨ0806ｨｺ06. 02ｨ ｨｲ02ｨｺｨｰ06ｨｬ ｨｪｨ 03ｨｨ01 ｨｨ09090502010609ｨ ｨｪｨｨｨｦ ｨ 0405ｨ 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨ 090606ｨ ｨｴ0209ｨｰ09ｨ 09 0706 ﾂ0902 0105ｨｨｨｰ0205ｨｹｨｪ060006 09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ060006 060704ｨｰｨ 000609ｨｺ060909ｨｺ06ｨｦ 090205ｨｹ09ｨｺ0601060407ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨ ｨｺｨ 0102ｨｬｨｨｨｨ, 04ｨ 05060302ｨｪｨｪ060006 09 1912 000601ｨｮ. 0004 070806ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ 0906040102ｨｦ09ｨｰ09ｨｨ02 ｨｪｨ 0706 ﾂ0902ｨｪｨｪ04ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬ 08ｨ 0405ｨｨ ﾂｨｪ0401 0906 ﾂ02ｨｰｨ ｨｪｨｨｨｦ ｨｰ080201 ｨ 000806ｨｰ0201ｨｪｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609 ｪ 0906040102050409ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804 (0502ｨｪ ｨｨ 0604ｨｨｨｬｨ 07 080603ｨｹ), 0902090606ｨ 060806ｨｰｨ ｨｨ ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ07 0706 ﾂ0904. 02ｨ ｨｪｨｪ0402 0706 ｨ ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ06 ｨ 0405ｨｨ 070605ｨｮ ﾂ02ｨｪ04 0708ｨｨ 0706ｨｬ06ｨｴｨｨ ｨｬ02ｨｰ0601ｨ 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07. 06ｨ ｨｺ0302 ｨｬ04 0708ｨｨｨｬ02ｨｪｨｨ05ｨｨ 0608ｨｨ00ｨｨｨｪｨ 05ｨｹｨｪ04ｨｦ 070601010601 ｨｺ ｨ ｨｪｨ 05ｨｨ04ｨｮ 01ｨ ｨｪｨｪ0401 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｪ ｨｬ02ｨｰ0601 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03. 09040506 0706ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 09 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬ 06ｨｰｨｪ060302ｨｪｨｨｨｨ 0008ｨｮ0707ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ010907 070601 0905ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 00ｨ ｨｺｨｰ06080609, 0708ｨｨｨｬ0208ｨｪ06 08ｨ 09ｨｪｨ 0008ｨｮ070702 ｨｪ02 ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ010907 070601 0906040102ｨｦ09ｨｰ09ｨｨ02ｨｬ 05ｨｰｨｨ01 00ｨ ｨｺｨｰ06080609. 0201ｨｪｨ ｨｺ06 05ｨｰｨｨ 0008ｨｮ070704 09ｨｨ05ｨｹｨｪ06 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ 090609ｨｰｨ 09ｨｮ: 0008ｨｮ0707ｨ, 0706010902080302ｨｪｨｪｨ 07 0905ｨｨ07ｨｪｨｨ06, ｨ 0405ｨ ｨ 06050202 0601ｨｪ06080601ｨｪｨ. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｬ0603ｨｪ06 ｨｮｨｰ0902080301ｨ ｨｰｨｹ, ﾂｨｰ06 0906040102ｨｦ09ｨｰ09ｨｨ06 07060109020800ｨ 02ｨｰ0907 ｨｪ02 06 ﾂ02ｨｪｨｹ ｨ 0605ｨｹ03ｨ 07 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨ ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 0108ｨｮ00ｨｨ01 0609ｨｰｨ 02ｨｰ0907 ｨ 06050202 ｨｨ05ｨｨ ｨｬ02ｨｪ0202 09ｨｰｨ ｨ ｨｨ05ｨｹｨｪ06ｨｦ. 000407090502ｨｪ04 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺｨｨ02 0008ｨｮ070704 060800ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ ｨｺｨ 030106ｨｬｨｮ ｨｨ04 ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 00ｨ ｨｺｨｰ06080609. 0308020105ｨ 00ｨ 02ｨｬ04ｨｦ ｨｪｨ ｨｬｨｨ 070601010601 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03 01ｨ 02ｨｰ 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 090606ｨｰｨｪ0209ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 09 0102ｨｦ09ｨｰ09ｨｨ02ｨｬ 0607080201020502ｨｪｨｪ060006 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ. 080506 ﾂ02090402 09050609ｨ : ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬ 0706 ﾂ0904, 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ0602 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ02. 0205ｨｨｨｰ0205ｨｹｨｪ0402 07060502090402 060704ｨｰ04 ｨｨ09070605ｨｹ04ｨｮ06ｨｰ0907 0906 09090201 09ｨｰ08ｨ ｨｪｨ 01 ｨｬｨｨ08ｨ 010507 ｨｺ06ｨｬ070502ｨｺ09ｨｪ0401 ｨｨ09090502010609ｨ ｨｪｨｨｨｦ 09 06ｨ 05ｨ 09ｨｰｨｨ ｨ 000806ｨｰ0201ｨｪｨｨｨｺｨｨ ｨｨ 0905ｨｨ07ｨｪｨｨ07 08ｨ 0405ｨｨ ﾂｨｪ0401 00ｨ ｨｺｨｰ06080609 ｨｪｨ ｨｮ080603ｨ ｨｦｨｪ0609ｨｰｨｹ. 00ｨｪ060006ｨｺ08ｨ ｨｰｨｪｨ 07 090609070806ｨｨ04090601ｨｨｨｬ0609ｨｰｨｹ 05ｨｰｨｨ01 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰ0609 0906 090802ｨｬ02ｨｪｨｨ 0708ｨｨ090601ｨｨｨｰ ｨｺ ｨｮ09ｨｰｨ ｨｪ06090502ｨｪｨｨ06 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06 ｨｪ06090401 04ｨ ｨｺ06ｨｪ06ｨｬ0208ｨｪ0609ｨｰ02ｨｦ, ｨｺ06ｨｰ06080402 ｨｪ02090604ｨｬ0603ｨｪ06 06070802010205ｨｨｨｰｨｹ 0708ｨｨ 07080609020102ｨｪｨｨｨｨ ｨｺ08ｨ ｨｰｨｺ06090806 ﾂｨｪ0401 060704ｨｰ0609. 05ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ 060704ｨｰ 000609ｨｺ060909ｨｺ06ｨｦ 090205ｨｹ09ｨｺ0601060407ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨ ｨｺｨ 0102ｨｬｨｨｨｨ (04060909ｨｨｨｦ09ｨｺｨｨｨｦ 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ04ｨｦ ｨ 0008ｨ 08ｨｪ04ｨｦ ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ00050908 ｨｨｨｬ. 08.06. 06ｨｨｨｬｨｨ0807040209ｨ ), ｨｺ06ｨｰ060804ｨｦ ｨｬ04 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 09 ｨｪｨ 0302ｨｬ ｨｨ09090502010609ｨ ｨｪｨｨｨｨ, ｨ 0405 04ｨ 05060302ｨｪ 09 1912 000601ｨｮ 08.01. 0206070802ｨｪｨｺ06. 05 ｨｬ06ｨｬ02ｨｪｨｰｨ 0609ｨｪ0609ｨ ｨｪｨｨ07 05ｨｰ06ｨｰ 060704ｨｰ 06ｨｰ05ｨｨ ﾂｨ 050907 06ｨｰ 07080201030209ｨｰ09ｨｮ06ｨｴｨｨ01 ｨｬｨｪ06000600ｨ ｨｺｨｰ0608ｨｪ06ｨｦ 090102ｨｬ06ｨｦ: 09 ｨｺ06ｨｬ070502ｨｺ0902 ｨｨ04ｨｮ- ﾂｨ 050609ｨｹ 0905ｨｨ07ｨｪｨｨ02 ｨｮ0106ｨ 0802ｨｪｨｨｨｦ, ｨｬ06ｨｪ06ｨｺｨｮ05ｨｹｨｰｨｮ08 ｨｨ 070605ｨｪ060202ｨｪｨｪ060006 0902090606ｨ 060806ｨｰｨ. 04ｨ ｨｪ0202 ｨｨ09090502010609ｨ ｨｪｨｨ07 ｨ ｨｨ0608ｨ 04ｨｪ0606ｨ 08ｨ 04ｨｨ07 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 070806090601ｨｨ05ｨｨ09ｨｹ 070609020906ｨｬ 0706 ﾂ0902ｨｪｨｪ06ｨｦ 09ｨｮ090702ｨｪ04ｨｨｨｨ ｨｪｨ 07ｨｨｨｰｨ ｨｰ0205ｨｹｨｪ0402 0908020104. 00 ｨｪｨ 09ｨｰ0607ｨｴ0202 090802ｨｬ07 ｨｪｨ ｨｮｨｺｨ 08ｨ 09070605ｨ 00ｨ 02ｨｬ ｨｬ02ｨｰ0601ｨ ｨｬｨｨ, ｨｺ06ｨｰ06080402 0706040906050706ｨｰ ｨ 06050202 070605ｨｪ06 0601ｨ 08ｨ ｨｺｨｰ0208ｨｨ040609ｨ ｨｰｨｹ 090609ｨｰｨ 09 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ, ｨｮ ﾂｨｨｨｰ0409ｨ 07 ｨｪ02 ｨｰ0605ｨｹｨｺ06 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ08ｨｮ02ｨｬ0402, ｨｪ06 ｨｨ ｨｪ02ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ08ｨｮ02ｨｬ0402 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ04 (1-4). 0201ｨｪｨ ｨｺ06 02ｨｴ02 ｨｪ0201ｨ 09ｨｪ06 0607080201020502ｨｪｨｨ02 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ060006 090609ｨｰｨ 09ｨ ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 05ｨｰｨｨ01 0708ｨｨ02ｨｬ0609 ｨ 040506 010609ｨｰｨ ｨｰ06 ﾂｨｪ06 ｨｰ08ｨｮ010602ｨｬｨｺｨｨｨｬ ｨｨ 0106080600ｨｨｨｬ. 060802ｨ 0609ｨ 05ｨｨ09ｨｹ 070806020201ｨｮ0804 ｨｺ0506ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｨ 070609050201ｨｮ06ｨｴ020006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 06ｨｰ010205ｨｹｨｪ0401 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ﾂｨｰ06 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ06 060008ｨ ｨｪｨｨ ﾂｨｨ09ｨ 0506 ｨｺｨ ｨｺ ﾂｨｨ090506 09040709050702ｨｬ0401 09ｨｨ010609, ｨｰｨ ｨｺ ｨｨ 01ｨｨｨ 07ｨ 0406ｨｪ ｨｨ0909050201ｨｮ02ｨｬ0401 ｨｬ0209ｨｰ 06ｨ ｨｨｨｰｨ ｨｪｨｨ07 (5, 6). 0308ｨｨｨｬ02ｨｪ02ｨｪｨｨ02 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ06 08ｨ 0903ｨｨ08ｨｨ0506 090604ｨｬ0603- * 04ｨ ｨ 06ｨｰｨ 0904070605ｨｪ02ｨｪｨ 0708ｨｨ 07060101020803ｨｺ02 00ｨｨｨｪｨｨ09ｨｰ020809ｨｰ09ｨ 06ｨ 08ｨ 040609ｨ ｨｪｨｨ07 ｨｨ ｨｪｨ ｨｮｨｺｨｨ 0408, 0108 01 16.552.11.7047 ｨｨ 0008ｨ ｨｪｨｰｨ 04080806 12-04-01371-ｨ. 85
13ｨｪ0609ｨｰｨｨ ｨｬｨ 0903ｨｰｨ ｨ ｨｪ060006 ｨｨ04ｨｮ ﾂ02ｨｪｨｨ07 ｨｬｨｪ060006ｨｺ06ｨｬ0706ｨｪ02ｨｪｨｰｨｪ0401 05ｨｺ0609ｨｨ09ｨｰ02ｨｬ, 09ｨｺ0506 ﾂｨ 07 0706 ﾂ0902ｨｪｨｪ0402 ｨｬｨｨｨｺ0806ｨ 060202ｨｪ060404. 00 ｨｰ06 0302 090802ｨｬ07 080204ｨｮ05ｨｹｨｰｨ ｨｰ04 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｨｨｬ0206ｨｰ 06 ﾂ02ｨｪｨｹ 09050603ｨｪｨｮ06 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｮ, 09 ｨｰ08ｨｮ0106ｨｬ 07060109020800ｨ 06ｨｰ0907 ｨ ｨｪｨ 05ｨｨ04ｨｮ, ｨｨ 070605ｨｰ06ｨｬｨｮ 010507 ｨｰ060006, ﾂｨｰ06ｨ 04 08ｨ 0406ｨ 08ｨ ｨｰｨｹ0907 09 09ｨｨ01060906ｨｬ ｨｬｨｪ06000606ｨ 08ｨ 04ｨｨｨｨ, ｨｪ0206ｨ 010601ｨｨｨｬ06 ｨｪｨ 010601ｨｨｨｰｨｹ ｨｪ06090402 07060101060104 ｨｺ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨ 02ｨｨｨｨ 01ｨ ｨｪｨｪ0401. 00 0708020109ｨｰｨ 09050702ｨｬ06ｨｦ 08ｨ ｨ 06ｨｰ02 ｨｬ04 0708020105ｨ 00ｨ 02ｨｬ 0608ｨｨ00ｨｨｨｪｨ 05ｨｹｨｪ04ｨｦ 070601010601 ｨｺ ｨ ｨｪｨ 05ｨｨ04ｨｮ 01ｨ ｨｪｨｪ0401 (ｨｬ02ｨｰ0601 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03), ｨｪｨ 0708ｨ 090502ｨｪｨｪ04ｨｦ ｨｪｨ 090407090502ｨｪｨｨ02 060906ｨ 02ｨｪｨｪ0609ｨｰ02ｨｦ ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0804 0706 ﾂ0902ｨｪｨｪ060006 ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｬｨ, 09090704ｨ ｨｪｨｪ0401 09 0102ｨｦ09ｨｰ09ｨｨ02ｨｬ 0607080201020502ｨｪｨｪ060006 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ. 05ｨｬ040905 ｨｬ02ｨｰ0601ｨ 04ｨ ｨｺ0506 ﾂｨ 02ｨｰ0907 09 09060401ｨ ｨｪｨｨｨｨ (02ｨｴ02 ｨｪｨ 05ｨｰｨ 0702 06ｨｰｨ 0608ｨ 070806ｨ ) ｨｰｨ ｨｺ060006 ｨｪｨ ｨ 0608ｨ 06ｨ 08ｨ 040609, ﾂｨｰ06ｨ 04 ｨｨ01 ｨｬ0603ｨｪ06 ｨ 040506 08ｨ 04010205ｨｨｨｰｨｹ ｨｪｨ 0008ｨｮ070704, ｨｺ06ｨｰ06080402 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ ｨ 04 ｨｰ0605ｨｹｨｺ06 0706 0601ｨｪ06ｨｬｨｮ ｨｨ04 0102ｨｦ09ｨｰ09ｨｮ06ｨｴｨｨ01 05ｨｺ06050600ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609, ｨ 0706 090902ｨｬ 0108ｨｮ00ｨｨｨｬ ｨ 0405ｨｨ ｨ 04 09010601ｨｪ04. 06ｨｬ02ｨｪｨｪ06 ｨｰｨ ｨｺｨ 07 090102ｨｬｨ 06ｨｰｨ 0608ｨ 070806ｨ 070604090605ｨｨｨｰ 0607080201020507ｨｰｨｹ 0008ｨｮ070704 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｬｨ 08ｨｺｨｨ08ｨｮ06ｨｴｨｨ02 ｨｪｨ ｨｨｨ 06050202 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ0402 08ｨ 0405ｨｨ ﾂｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｺ06ｨｰ06080402 09040409ｨ ｨｪ04 0905ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ0909050201ｨｮ02ｨｬ060006 00ｨ ｨｺｨｰ0608ｨ. 0308ｨｨ 0906070609ｨｰｨ 090502ｨｪｨｨｨｨ 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ0401 0706 0904ｨ 08ｨ ｨｪｨｪ06ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｬ0603ｨｪ06 09040709ｨｨｨｰｨｹ ｨｰ02 ｨｰｨ ｨｺ0906ｨｪ04, ｨｺ06ｨｰ06080402 0102ｨｬ06ｨｪ09ｨｰ08ｨｨ08ｨｮ06ｨｰ ｨｪｨ ｨｨｨ 06050202 04ｨｪｨ ﾂｨｨｨｬ0402 08ｨ 0405ｨｨ ﾂｨｨ07. 000205ｨｹ06 08ｨ ｨ 06ｨｰ04 ｨ 040506 ｨｨ09090502010609ｨ ｨｪｨｨ02 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 09 0706 ﾂ09ｨ 01 0105ｨｨｨｰ0205ｨｹｨｪ060006 09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ060006 060704ｨｰｨ ｨｬ02ｨｰ060106ｨｬ 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 09 0708ｨｨｨｬ02ｨｪ02ｨｪｨｨ02ｨｬ 0708020105060302ｨｪｨｪ060006 ｨｪｨ ｨｬｨｨ 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03. 0002ｨｰ0601ｨｨｨｺｨ. 0502ｨｬ0205ｨｹｨｪ04ｨｦ ｨｮ ﾂｨ 09ｨｰ06ｨｺ ｨｬｨｪ0600060502ｨｰｨｪ020006 060704ｨｰｨ 04010807 ｪ00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ 070506ｨｴｨ 01ｨｹ06 06ｨｺ060506 1,5 00ｨ 09 ｨｮｨｺ0506ｨｪ06ｨｬ 1 ｨｪｨ 090209020806-04ｨ 07ｨ 01 08ｨ 09070605060302ｨｪ ｨｪｨ 0603ｨｪ06ｨｦ 06ｨｺ08ｨ ｨｨｨｪ02 0805ｨｨｨｪ09ｨｺ06-02ｨｬｨｨｨｰ08060909ｨｺ06ｨｦ 09060409040302ｨｪｨｪ0609ｨｰｨｨ, 0708020109ｨｰｨ 090502ｨｪｨｪ06ｨｦ ｨｬ060802ｨｪｨｪ06ｨｦ 08ｨ 09ｨｪｨｨｨｪ06ｨｦ. 03080209040302ｨｪｨｨ02 ｨｪｨ 01 ｨｮ080609ｨｪ02ｨｬ ｨｬ060807 162 ｨｬ. 05080201ｨｪ02ｨｬｨｪ0600060502ｨｰｨｪ0202 ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 0609ｨ 01ｨｺ0609 06ｨｺ060506 600 ｨｬｨｬ 09 000601, ｨｨ04 ｨｪｨｨ01 0708ｨｨｨｬ0208ｨｪ06 0706050609ｨｨｨｪｨ ｪ 04ｨ ｨｬｨ ｨｦ ｪｨ 0900ｨｮ09ｨｰ, 09080201ｨｪ020006010609ｨ 07 ｨｰ02ｨｬ070208ｨ ｨｰｨｮ08ｨ 4,1 縺05. 0306 ﾂ09ｨ 010208ｨｪ060906-070601040605ｨｨ09ｨｰｨ 07, 09ｨｮ070209 ﾂｨ ｨｪｨ 07. 02ｨｰｨ 0608 0706 ﾂ0902ｨｪｨｪ0401 06ｨ 08ｨ 04020609 070806090601ｨｨ05ｨｨ 09 ｨｨ06ｨｪ02 2010 000601ｨ 09 0005ｨｮｨ ｨｨｨｪ04 07ｨ 0106ｨｰｨｪ060006 000608ｨｨ0406ｨｪｨｰｨ 081 (20 09ｨｬ) 09 090609ｨｹｨｬｨｨ 09ｨ 08ｨｨｨ ｨｪｨｰｨ 01 060704ｨｰｨ (09 04ｨ 09ｨｨ09ｨｨｨｬ0609ｨｰｨｨ 06ｨｰ 0906 ﾂ02ｨｰｨ ｨｪｨｨ07 0906040102ｨｦ09ｨｰ09ｨｮ06ｨｴｨｨ01 ｨ 000806ｨｰ0201ｨｪｨｨ- ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609). 020507 090401020502ｨｪｨｨ07 020108 09 070806ｨ ｨｨ08ｨｺｨｮ 06ｨ ｨｲ02ｨｬ06ｨｬ 2 ｨｬ05 06ｨｰｨ ｨｨ08ｨ 05ｨｨ ｨｪｨ 090209ｨｺｨｮ 0706 ﾂ0904 (0,2 00). 0206ｨ ｨ 09050705ｨｨ 08ｨ 09ｨｪ0602 0706 06ｨ ｨｲ02ｨｬｨｮ ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 03ｨ 08ｨｨｨｺ0609 01ｨｨｨ ｨｬ02ｨｰ0806ｨｬ 0,1 ｨｬｨｬ (00Innomed03, 0002ｨｪ0008ｨｨ07), 350 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 08 (ｨｪｨ ｨｰ08ｨｨｨｦ-00060900ｨ ｨｰｨｪ04ｨｦ ｨ ｨｮ000208 ｪ 20 ｨｬM, ｨｨ0406ｨｰｨｨ0602ｨｨｨ ｨｪｨ ｨｰ 00ｨｮｨ ｨｪｨｨ01ｨｨｨｪｨ ｪ 240 ｨｬM; 0801 7,0), 350 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 09 (0608ｨｨ09-01Cl ｪ 500 ｨｬM, SDS ｪ 1 % 0706 ｨｬｨ 090902 ｨｺ 06ｨ ｨｲ02ｨｬｨｮ; ph 7,0) ｨｨ 400 ｨｬｨｺ05 09ｨｬ0209ｨｨ 0002ｨｪ0605-0105060806000608ｨｬ. 030806ｨ ｨｨ08ｨｺｨｮ 0706ｨｬ02ｨｴｨ 05ｨｨ 0906 0909ｨｰ080701ｨｨ09ｨ ｨｰ0205ｨｹ ｨｨ 08ｨ 0408ｨｮ03ｨ 05ｨｨ 06ｨ 08ｨ 040202 09 ｨｰ02 ﾂ02ｨｪｨｨ02 10-15 ｨｬｨｨｨｪ. 05ｨ ｨｰ02ｨｬ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 0708ｨｨ 10 000-15 000 g 09 ｨｰ02 ﾂ02ｨｪｨｨ02 5 ｨｬｨｨｨｪ. 000601ｨｪｨｮ06 00ｨ 04ｨｮ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ, 0106ｨ ｨ 09050705ｨｨ 400 ｨｬｨｺ05 0105060806000608ｨｬｨ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ06 0909ｨｰ080701ｨｨ09ｨ 05ｨｨ 1-3 ｨｬｨｨｨｪ, 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｰｨ ｨｺ 0302, ｨｺｨ ｨｺ ｨｪｨ 070802010401ｨｮｨｴ02ｨｦ 09ｨｰｨ 01ｨｨｨｨ, 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 090601ｨｪｨｮ06 00ｨ 04ｨｮ. 08 ｨｪ0206 ﾂｨｨｨｴ02ｨｪｨｪ06ｨｬｨｮ 05ｨｺ09ｨｰ08ｨ ｨｺｨｰｨｮ 020108 0106ｨ ｨ 09050705ｨｨ 0601ｨｨｨｪ 06ｨ ｨｲ02ｨｬ ｨｨ040607080607ｨｨ050609060006 0907ｨｨ08ｨｰｨ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ06 0909ｨｰ080701ｨｨ09ｨ 05ｨｨ, 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ, 070806ｨｬ0409ｨ 05ｨｨ 70 % 05ｨｰｨ ｨｪ060506ｨｬ, 09050200ｨｺｨ 07060109ｨｮ03ｨｨ09ｨ 05ｨｨ ｨｪｨ 09060401ｨｮ0102 ｨｨ 08ｨ 09ｨｰ0906080705ｨｨ 0609ｨ 0106ｨｺ 0708ｨｨ 65 縺05 09 ｨｰ02 ﾂ02ｨｪｨｨ02 15 ｨｬｨｨｨｪ 09 100 ｨｬｨｺ05 09060104. 020507 06 ﾂｨｨ09ｨｰｨｺｨｨ 020108 06ｨｰ 0708ｨｨｨｬ020902ｨｦ 070806090601ｨｨ05ｨｨ 050502ｨｺｨｰ08060006080204 09 1 % ｨ 00ｨ 08060402 (0,505 060803-ｨ ｨｮ000208). 0004080204ｨ ｨｪｨｪ04ｨｦ ｨ 0506ｨｺ, 090601020803ｨ ｨｴｨｨｨｦ 020108, 0706ｨｬ02ｨｴｨ 05ｨｨ 09 070806ｨ ｨｨ08ｨｺｨｮ ｨｰｨｨ07ｨ 05070702ｨｪ01060800 (1,5 ｨｬ05), 0106ｨ ｨ 09050705ｨｨ 2 06ｨ ｨｲ02ｨｬｨ 08ｨ 09ｨｰ090608ｨ 00 (ｨｨ0406ｨｰｨｨ0602ｨｨｨ ｨｪｨ ｨｰ 00ｨｮｨ ｨｪｨｨ01ｨｨｨｪｨ ｪ 3 00, 0608ｨｨ09-HCl ｪ 20 ｨｬM, 0608ｨｨｨｰ06ｨｪ X-100 ｪ 20 ｨｬ00/ｨｬ05; 0801 7,0) ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 0708ｨｨ 65 縺05 0106 070605ｨｪ060006 08ｨ 09ｨｰ09060802ｨｪｨｨ07 ｨ 0506ｨｺｨ. 08 08ｨ 09ｨｰ090608ｨｮ 0708ｨｨ05ｨｨ09ｨ 05ｨｨ 20 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 01 (08ｨ 09ｨｰ090608 00 09 0106ｨ ｨ 090502ｨｪｨｨ02ｨｬ ｨｰ06ｨｪｨｺ0601ｨｨ0907020809ｨｪ06ｨｦ 06ｨｺｨｨ09ｨｨ ｨｺ0802ｨｬｨｪｨｨ07, 40 ｨｬ00/ｨｬ05), 07020802ｨｬ0203ｨｨ09ｨ 05ｨｨ ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ - 86
1305ｨｨ 5 ｨｬｨｨｨｪ 0708ｨｨ ｨｺ06ｨｬｨｪｨ ｨｰｨｪ06ｨｦ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802, 070208ｨｨ0601ｨｨ ﾂ0209ｨｺｨｨ 0909ｨｰ080701ｨｨ09ｨ 07. 05ｨ ｨｰ02ｨｬ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ 0708ｨｨ ｨｬｨ ｨｺ09ｨｨｨｬｨ 05ｨｹｨｪ06ｨｦ 09ｨｺ06080609ｨｰｨｨ, 070605ｨｪ0609ｨｰｨｹ06 ｨｮｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, 0609ｨ 0106ｨｺ 09ｨｮ090702ｨｪ01ｨｨ080609ｨ 05ｨｨ 09 200 ｨｬｨｺ05 08ｨ 09ｨｰ090608ｨ 02 (05ｨｰｨ ｨｪ0605 ｪ 25 %, ｨｨ040607080607ｨ ｨｪ0605 ｪ 25 %, NaCl ｪ 100 ｨｬM, 0608ｨｨ09-HCl ｪ 10 ｨｬM; 0801 7,0), 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ, 070605ｨｪ0609ｨｰｨｹ06 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, 0609ｨ 0106ｨｺ 080209ｨｮ090702ｨｪ01ｨｨ080609ｨ 05ｨｨ 09 05ｨｰｨ ｨｪ060502, 060707ｨｰｨｹ 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ 070605ｨｪ0609ｨｰｨｹ06 ｨｮ01ｨ 050705ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ. 0209ｨ 0106ｨｺ 07060109ｨｮ03ｨｨ09ｨ 05ｨｨ ｨｪｨ 09060401ｨｮ0102 09 ｨｰ02 ﾂ02ｨｪｨｨ02 15 ｨｬｨｨｨｪ, 0106ｨ ｨ 09050705ｨｨ 50 ｨｬｨｺ05 050506ｨｨ08ｨｮ06ｨｴ020006 ｨ ｨｮ000208ｨ (0608ｨｨ09-HCl ｪ 10 ｨｬM, EDTA ｪ 1 ｨｬM; 0801 8,0) ｨｨ 07060109020800ｨ 05ｨｨ ｨｮｨｬ020802ｨｪｨｪ06ｨｬｨｮ 0909ｨｰ080701ｨｨ09ｨ ｨｪｨｨ06 ｨｪｨ 090608ｨｰ02ｨｺ0902 09 ｨｰ02 ﾂ02ｨｪｨｨ02 30 ｨｬｨｨｨｪ. 0306090502 05ｨｰ060006 06ｨ 08ｨ 040204 0202ｨｪｨｰ08ｨｨ00ｨｮ00ｨｨ080609ｨ 05ｨｨ ｨｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 09ｨｮ070208ｨｪｨ ｨｰｨ ｨｪｨｰ, ｨｨ04ｨ 0200ｨ 07 070607ｨ 01ｨ ｨｪｨｨ07 06ｨｺｨｨ09ｨｨ ｨｺ0802ｨｬｨｪｨｨ07 09 06 ﾂｨｨｨｴ02ｨｪｨｪ04ｨｦ 07080207ｨ 08ｨ ｨｰ 020108. 0308ｨｨ 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨｨｨ ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ 06 ﾂｨｨｨｴ02ｨｪｨｪ04ｨｦ 07080207ｨ 08ｨ ｨｰ 020108 0905ｨｮ03ｨｨ05 ｨｬｨ ｨｰ08ｨｨ0202ｨｦ 09 0802ｨ ｨｺ02ｨｨｨｨ 030004 09 ｨｮｨｪｨｨ09020809ｨ 05ｨｹｨｪ04ｨｬｨｨ 0708ｨ ｨｦｨｬ0208ｨ ｨｬｨｨ ｨｺ 09ｨ 08ｨｨｨ ｨ 0205ｨｹｨｪ06ｨｬｨｮ ｨｮ ﾂｨ 09ｨｰｨｺｨｮ V4 0002ｨｪｨ 16S-08040108 (F515 ｪ GTGCCAGCMGCCGCGGTAA, R806 ｪ GGATACVSGGGTATCTAAT) (7) 09 0106ｨ ｨ 090502ｨｪｨｨ02ｨｬ 0605ｨｨ0006ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 ｨｨ0102ｨｪｨｰｨｨ00ｨｨｨｺｨ ｨｰ06080609 010507 ｨｺｨ 030106ｨｦ 070806ｨ 04 ｨｨ 0905ｨｮ0302ｨ ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｪ0206ｨ 010601ｨｨｨｬ0401 010507 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 0706 070806ｨｰ06ｨｺ0605ｨｮ 00Roche03 (010902ｨｦ02ｨ 08ｨｨ07). 0609070605ｨｹ04ｨｮ02ｨｬ0402 0708ｨ ｨｦｨｬ020804 ｨ 0405ｨｨ 09ｨｺ06ｨｪ09ｨｰ08ｨｮｨｨ080609ｨ ｨｪ04 ｨｪｨ 0609ｨｪ060902 ｨ ｨｪｨ 05ｨｨ04ｨ 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｺｨ ｨｺ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｰｨ ｨｺ ｨｨ ｨ 080102ｨｦ. 0306010006ｨｰ0609ｨｺｨｮ 070806ｨ ｨｨ 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ02 070806090601ｨｨ05ｨｨ ｨｪｨ 0708ｨｨｨ 060802 GS Junior (00Roche03, 010902ｨｦ02ｨ 08ｨｨ07) 09060005ｨ 09ｨｪ06 0802ｨｺ06ｨｬ02ｨｪ01ｨ 02ｨｨｨｨ 070806ｨｨ04090601ｨｨｨｰ020507. 020507 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ ｨｨ0102ｨｪｨｰｨｨ00ｨｨｨｺｨ 02ｨｨｨｨ 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ 0106070605ｨｪｨｨｨｰ0205ｨｹｨｪ06 ｨｰｨ ｨｺ0302 090609070605ｨｹ040609ｨ 05ｨｨ09ｨｹ ｨ ｨ 0406ｨｦ 01ｨ ｨｪｨｪ0401 RDP (Ribosomal Database Project, http://rdp.cme.msu.edu/). 0508ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｬｨｨｨｺ0806ｨ ｨｪ0401 090606ｨ ｨｴ0209ｨｰ09 0904070605ｨｪ0705ｨｨ 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ 080209ｨｮ0809ｨ VAMPS (Visualization and Analysis of Microbial Population Structure, 010609ｨｰｨｮ0702ｨｪ ｨｪｨ 09ｨ ｨｦｨｰ02 http://vamps.mbl.edu/). 05 0706ｨｬ06ｨｴｨｹ06 09ｨｨ09ｨｰ02ｨｬ04 VAMPS 070605ｨｮ ﾂｨｨ05ｨｨ ｨｰｨ ｨ 05ｨｨ0204, 090601020803ｨ ｨｴｨｨ02 04ｨｪｨ ﾂ02ｨｪｨｨ07 010605ｨｨ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0401 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 09 ｨｺｨ 030106ｨｦ 070806ｨ 02, ｨｪｨ 0609ｨｪ0609ｨ ｨｪｨｨｨｨ ｨｺ06ｨｰ06080401 ｨ 0405ｨｨ 070609ｨｰ080602ｨｪ04 ｨｨｨｰ060006090402 01ｨｨｨ 0008ｨ ｨｬｨｬ04. 0806ｨｬ07ｨｹ06ｨｰ0208ｨｪｨｮ06 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｮ 070605ｨｮ ﾂ02ｨｪｨｪ0401 09 080204ｨｮ05ｨｹｨｰｨ ｨｰ02 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｮ01ｨ 0502ｨｪｨｨ02 ｨｨ04 ｨｨ01 090609ｨｰｨ 09ｨ ｨｬ02ｨｰ06ｨｺ ｨｨ 0708ｨ ｨｦｨｬ02080609 0609ｨｮｨｴ0209ｨｰ09050705ｨｨ 09060005ｨ 09ｨｪ06 ｨｬ02ｨｰ0601ｨｨ ﾂ0209ｨｺｨｨｨｬ 0802ｨｺ06ｨｬ02ｨｪ01ｨ 02ｨｨ07ｨｬ 09 0708ｨｨ05060302ｨｪｨｨｨｨ Ribosome Database Project (RDP) Pipeline (8). 0004070605ｨｪ0705ｨｨ 090408ｨ 09ｨｪｨｨ09ｨ ｨｪｨｨ02 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, 070609ｨｰ080602ｨｪｨｨ02 ｨｬｨ ｨｰ08ｨｨ0204 0002ｨｪ02ｨｰｨｨ ﾂ0209ｨｺｨｨ01 08ｨ 0909ｨｰ0607ｨｪｨｨｨｦ ｨｨ ｨｺ05ｨ 09ｨｰ0208ｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ 09 0708ｨｨｨｬ02ｨｪ02ｨｪｨｨ02ｨｬ ｨ 05000608ｨｨｨｰｨｬｨ 00average neighbor03. 02ｨ 050202 070806090601ｨｨ05ｨｨ ｨｺ05ｨ 0909ｨｨ00ｨｨｨｺｨ 02ｨｨ06 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ ｨｪｨ OTU (Operational Taxonomic Unit) 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｺ08ｨｨｨｰ0208ｨｨ07 97 % 0901060109ｨｰ09ｨ 020507 0609ｨｮｨｴ0209ｨｰ090502ｨｪｨｨ07 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03 ｨｨ04 ｨｺｨ 030106ｨｦ ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ ｨ 08ｨ 05ｨｨ 0905ｨｮ ﾂｨ ｨｦｨｪｨｮ06 0904ｨ 0608ｨｺｨｮ ｨｨ04 985 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ, ｨｪ0608ｨｬｨ 05ｨｨ04ｨｨ08ｨｮ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ 010507 070609050201ｨｮ06ｨｴ020006 0908ｨ 09ｨｪ02ｨｪｨｨ07, ｨｨ 09060401ｨ 09ｨ 05ｨｨ 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ0402 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ 0706 ｨｺｨ 030106ｨｬｨｮ ｨｨ04 0904ｨ 08ｨ ｨｪｨｪ0401 00ｨ ｨｺｨｰ06080609. 09ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨｨ 0908ｨ 09ｨｪｨｨ09ｨ 05ｨｨ 09 0708ｨｨ05060302ｨｪｨｨｨｨ RDP 00LibCompare03 (http://rdp.cme.msu.edu/comparison/comp.jsp). 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ ｨ 0405ｨｨ 09000608ｨｬｨｨ080609ｨ ｨｪ04 ｨｰｨ ｨ 05ｨｨ0204 0706 ｨｺｨ 030106ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ, 090601020803ｨ ｨｴｨｨ02 01ｨ ｨｪｨｪ0402 06 04ｨｪｨ ﾂｨｨｨｬ0609ｨｰｨｨ 08ｨ 0405ｨｨ ﾂｨｨｨｦ ｨｬ020301ｨｮ 0109ｨｮｨｬ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺｨ ｨｬｨｨ, 06ｨ ｨｲ0201ｨｨｨｪ02ｨｪｨｪ04ｨｬｨｨ 0706 090606ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｴ02ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ. 0306080600 010507 060202ｨｪｨｺｨｨ 04ｨｪｨ ﾂｨｨｨｬ0609ｨｰｨｨ 0905ｨｨ07ｨｪｨｨ07 00ｨ ｨｺｨｰ0608ｨ 06070802010205ｨｨ05ｨｨ 08ｨ 09ｨｪ04ｨｬ 0,05 (090902 0706ｨｺｨ 04ｨ ｨｰ0205ｨｨ 09040302 05ｨｰ060006 0706080600ｨ ｨｪ02 06ｨｰ08ｨ 03ｨ 05ｨｨ 04ｨｪｨ ﾂｨｨｨｬ0401 08ｨ 0405ｨｨ ﾂｨｨｨｦ ｨｨ ｨｪｨ 06ｨ 060806ｨｰ). 040204ｨｮ05ｨｹｨｰｨ ｨｰ04. 02ｨ 08ｨ 040204 0706 ﾂ0904 09 09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ06ｨｬ 070605020906ｨｬ 060704ｨｰ02 04010807-00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ 06ｨｰｨ ｨｨ08ｨ 05ｨｨ 070601 0706090209ｨ ｨｬｨｨ 05ｨｹｨｪｨ ｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ, 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ ｨ 0204, 09 ｨｮ09050609ｨｨ0701 0902090606ｨ 060806ｨｰｨ ｨｨ ｨ 0204 ｨｪ020006 (ｨｰｨ ｨ 05.). 87
1309ｨ 08ｨ ｨｺｨｰ0208ｨｨ09ｨｰｨｨｨｺｨ ｨｨ09090502010609ｨ ｨｪｨｪ0401 06ｨ 08ｨ 04020609 0706 ﾂ0904 (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ 070605020906ｨｦ 060704ｨｰ 04060909ｨｨｨｦ09ｨｺ060006 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 ｨ 0008ｨ 08ｨｪ060006 ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ, 00. 000609ｨｺ09ｨ, ｨｨ06ｨｪｨｹ 2010 000601ｨ ) 01 ｧ爲 08ｨ 0402ｨ 0604090209ｨｰｨｺ0609ｨ ｨｪｨｨ02 0502090606ｨ 060806ｨｰ 08ｨｮ05ｨｹｨｰｨｮ08ｨ ﾁｨｨ090506 ｨｪｨｮｨｺ050206ｨｰｨｨ01ｨｪ0401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰ02ｨｦ 1 + + 040603ｨｹ 1208 2 6ﾓ1 + 040603ｨｹ 1613 3 + + 0902ｨｪ 2584 4 6ﾓ1 + 0902ｨｪ 1928 5 6ﾓ1 6ﾓ1 0902ｨｪ 2049 6 + 6ﾓ1 0902ｨｪ 985 7 6ﾓ1 6ﾓ1 040603ｨｹ 1342 8 + 6ﾓ1 040603ｨｹ 2002 0308ｨｨｨｬ02 ﾂｨ ｨｪｨｨ02. 00+03 ｨｨ 006ﾓ103 ｪ 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 ｨｪｨ 05ｨｨ ﾂｨｨ02 ｨｨ 06ｨｰ09ｨｮｨｰ09ｨｰ09ｨｨ02 0102ｨｦ09ｨｰ09ｨｮ06ｨｴ020006 00ｨ ｨｺｨｰ0608ｨ. 08 09 04ｨｨ09. 1. 04ｨ 0907080201020502ｨｪｨｨ02 00ｨｨ05 (08) ｨｨ 0806010609 (09) ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 0706 06ｨ 08ｨ 0402ｨ ｨｬ 0706 ﾂ0904 (0706 01ｨ ｨｪｨｪ04ｨｬ 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S-08040108): 1 ｪ 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ 0902090606ｨ 0806ｨｰ06ｨｬ, 2 ｪ 09 0902090606ｨ 060806ｨｰ06ｨｬ, 3 ｪ ｨ 0204 0902090606ｨ 060806ｨｰｨ, 4 ｪ 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨ 0204 0902090606ｨ 060806ｨｰｨ (070601 080603ｨｹ06); 5 ｪ 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｨ 0902090606ｨ 060806ｨｰ06ｨｬ, 6 ｪ 09 0902090606ｨ 060806ｨｰ06ｨｬ, 7 ｪ ｨ 0204 0902090606ｨ 060806ｨｰｨ, 8 ｪ 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨ 0204 0902090606ｨ 060806ｨｰｨ (07060106 05ｨｹｨｪ06ｨｬ) (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ 070605020906ｨｦ 060704ｨｰ 04060909ｨｨｨｦ09ｨｺ060006 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 ｨ 0008ｨ 08ｨｪ060006 ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ, 00. 000609ｨｺ09ｨ, ｨｨ06ｨｪｨｹ 2010 000601ｨ ). 88 00 090609ｨｰｨ 0902 090606ｨ ｨｴ0209ｨｰ09ｨ ｨ 0405ｨｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪ04 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ 19 00ｨｨ05 ｨｬｨｨｨｺ-
130806060800ｨ ｨｪｨｨ04ｨｬ0609 (08ｨｨ09. 1, 08), 09080201ｨｨ ｨｺ06ｨｰ06080401 ｨ 080102ｨｨ 090609ｨｰｨ 09050705ｨｨ ｨｪ02ｨ 0605ｨｹ03ｨｮ06 01060506 (06ｨｰ 3 0106 8 % 09 04ｨ 09ｨｨ09ｨｨｨｬ0609ｨｰｨｨ 06ｨｰ 06ｨ 08ｨ 0402ｨ ). 0308ｨｨ 05ｨｰ06ｨｬ 09050201ｨｮ02ｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ 09ｨｪｨｨ0302ｨｪｨｨ02 ﾂｨｨ0905ｨ ｨ 080102ｨｦ 09 06ｨ 08ｨ 0402ｨ 01 09 ｨ 020909ｨｬ02ｨｪｨｪ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ. 05080201ｨｨ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ ｨｪｨ ｨｨｨ 06050202 03ｨｨ0806ｨｺ06 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 0708020109ｨｰｨ 090502ｨｪｨ 00ｨｨ05ｨ Proteobacteria 09 09ｨｺ05ｨ 0106ｨｬ 09 090606ｨ ｨｴ0209ｨｰ0906 06ｨｺ060506 50 %, 04ｨ ｨｪ02ｨｦ 090502010609ｨ 05ｨｨ Actinobacteria (06ｨｺ060506 20 %), Acidobacteria (5 %) ｨｨ Firmicutes (5 %). 02060507 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｬ0401 0106 00ｨｨ0504, 090609ｨｰｨ 09050705ｨ 0708ｨｨｨｬ0208ｨｪ06 15-20 %. 000603ｨｪ06 ｨｰｨ ｨｺ0302 0904010205ｨｨｨｰｨｹ ｨｪ0209ｨｺ0605ｨｹｨｺ06 00ｨｨ05, ｨｪｨ ｨｺ06ｨｰ06080402 09 090606ｨ ｨｴ0209ｨｰ0902 0708ｨｨ010601ｨｨ050609ｨｹ 06ｨｺ060506 1 % ｪ 05ｨｰ06 Crenarcheota, Verrucomicrobia, Bacteroidetes ｨｨ Gemmatimonadetes. ﾁｨｨ090502ｨｪｨｪ0609ｨｰｨｹ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ04 0609ｨｰｨ 05ｨｹｨｪ0401 00ｨｨ05 09 090606ｨ ｨｴ0209ｨｰ09ｨ 01 ｨｪ02 010609ｨｰｨｨ00ｨ 05ｨ 1 %. 0009020006 ｨｬ04 09040709ｨｨ05ｨｨ 06ｨｺ060506 300 0806010609 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 (09ｨｬ. 08ｨｨ09. 1, 09), 0708ｨｨ ﾂ02ｨｬ ｨ 0605ｨｹ03ｨｮ06 01060506 ｨｨ04 ｨｪｨｨ01 (06ｨｺ060506 40-50 %) 090609ｨｰｨ 09050705ｨｨ ｨｰ02, 010507 ｨｺ06ｨｰ06080401 0706090502010609ｨ ｨｰ0205ｨｹｨｪ0609ｨｰｨｨ 020108 ｨｪ02 ｨｮ01ｨ 050609ｨｹ ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｰｨｹ 0106 080601ｨ. 0308ｨｨ 08ｨ 0909ｨｬ06ｨｰ0802ｨｪｨｨｨｨ 090606ｨ ｨｴ0209ｨｰ09ｨ ｨｪｨ ｨｮ080609ｨｪ02 080601ｨ 09 00ｨｨ0502 Proteobacteria 0106ｨｬｨｨｨｪｨｨ080609ｨ 05ｨｨ 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ Rhizobium (ｨｨ01 090601020803ｨ ｨｪｨｨ02 09ｨ 08ｨｹｨｨ080609ｨ 0506 09 070802010205ｨ 01 10-20 %), Sphingomonas sp., Pseudomonas sp., ｨ ｨｰｨ ｨｺ0302 ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ08ｨｮ02ｨｬ0402 0106 080601ｨ 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ ｨｺ05ｨ 0909ｨ Proteobacteria. 00 06ｨ 08ｨ 0402ｨ 01 070601 0706090209ｨ ｨｬｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ 0708060202ｨｪｨｰ 08ｨｨ0406ｨ ｨｨｨｦ 09 0706 ﾂ0902 09ｨｨ05ｨｹｨｪ06 09ｨ 08ｨｹｨｨ080609ｨ 05. 0006040102050409ｨ ｨｪｨｨ02 0604ｨｨｨｬ06ｨｦ 0803ｨｨ 09 0902090606ｨ 060806ｨｰ02 0708ｨｨ090601ｨｨ0506 ｨｺ ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ06 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ 08ｨｨ0406ｨ ｨｨｨｦ, 09 ｨｰ06 090802ｨｬ07 ｨｺｨ ｨｺ 0502ｨｪ ｨｪ02 06ｨｺｨ 040409ｨ 05 ｨｰｨ ｨｺ060006 0905ｨｨ07ｨｪｨｨ07 ｨｪｨｨ 09 ｨｮ09050609ｨｨ0701 0902090606ｨ 060806ｨｰｨ, ｨｪｨｨ ｨ 0204 ｨｪ020006. 00 02020506ｨｬ ｨｬ0603ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ ｨ 06050202 09040906ｨｺｨｮ06 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 08ｨｨ0406ｨ ｨｨｨｦ 09 0902090606ｨ 060806ｨｰ02, ﾂｨｰ06 09070605ｨｪ02 06ｨ ｨｲ0709ｨｪｨｨｨｬ06, 070609ｨｺ0605ｨｹｨｺｨｮ 0601ｨｪ06ｨｦ ｨｨ04 ｨｺｨｮ05ｨｹｨｰｨｮ08 09 ｨｪ02ｨｬ ｨ 0405 ｨｺ0502090208. 00 00ｨｨ0502 Actinobacteria ｨ 0605ｨｹ03ｨｮ06 01060506 04ｨ ｨｪｨｨｨｬｨ 05ｨｨ ｨｪ02ｨｨ04090209ｨｰｨｪ0402 08060104 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ04 0902ｨｬ02ｨｦ09ｨｰ09 Acidimicrobidae ｨｨ Actinobacteridae, ｨ ｨｰｨ ｨｺ0302 04ｨ ｨｬ02ｨｰｨｪ04ｨｦ 09ｨｺ05ｨ 01 09ｨｪ0609ｨｨ05ｨｨ Arthrobacter sp. (070601 0706090209ｨ ｨｬｨｨ 05ｨｹｨｪｨ 020006 090601020803ｨ ｨｪｨｨ02 06ｨｺｨ 04ｨ 050609ｨｹ 09040302, ﾂ02ｨｬ 070601 0706090209ｨ ｨｬｨｨ 0604ｨｨｨｬ06ｨｦ 0803ｨｨ), Solirubrobacter sp., Terrabacter sp. ｨｨ Nocardioides sp. 05080201ｨｨ Acidobacteria 0106ｨｬｨｨｨｪｨｨ080609ｨ 05ｨｨ 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ 0806010609 GP1, GP16, GP3, GP4 ｨｨ GP6. 020507 09090201 06ｨ 08ｨ 04020609 ｨ 040506 01ｨ 08ｨ ｨｺｨｰ0208ｨｪ06 06ｨ ｨｴ0202 09ｨｪｨｨ0302ｨｪｨｨ02 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ Acidobacteria 0708ｨｨ ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨｨｨ 0706 ﾂ0904. 08ｨ ｨｺ 06ｨｰｨｬ02 ﾂｨ 06ｨｰ C.L. Lauber 09 0906ｨ 09ｨｰ. (9), ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ04 ｨｨ04 00ｨｨ0504 Acidobacteria 0708020108ｨ 09070605060302ｨｪ04 ｨｺ 06ｨ ｨｨｨｰｨ ｨｪｨｨ06 0708ｨｨ ｨ 06050202 ｨｺｨｨ09050401 04ｨｪｨ ﾂ02ｨｪｨｨ0701 ph, ｨｨ 05ｨｰｨｨｨｬ ｨｬ0603ｨｪ06 06ｨ ｨｲ0709ｨｪｨｨｨｰｨｹ 09ｨｪｨｨ0302ｨｪｨｨ02 ｨｨ01 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ 09 06ｨ 08ｨ 0402ｨ 01 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ 0706 ﾂ0904. 090605ｨｹ03ｨｨｨｪ09ｨｰ0906 0708020109ｨｰｨ 09ｨｨｨｰ020502ｨｦ 00ｨｨ0504 Firmicutes 090609ｨｰｨ 09050705ｨｨ ｨｪ02 ｨｨ0102ｨｪｨｰｨｨ00ｨｨ02ｨｨ080609ｨ ｨｪｨｪ0402 0106 080601ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ04 ｨｨ04 ｨｺ05ｨ 09090609 Bacilli ｨｨ Clostridia, ｨ ｨｰｨ ｨｺ0302 08060104 Paenibacillus sp. ｨｨ Bacillus sp. 08ｨｪｨ 05ｨｨ04 01ｨ ｨｪｨｪ0401 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 0706ｨｺｨ 04ｨ 05, ﾂｨｰ06 0706 ﾂ0902ｨｪｨｪ0602 ｨｬｨｨｨｺ0806ｨ ｨｪ0602 090606ｨ ｨｴ0209ｨｰ0906 06ｨ 05ｨ 01ｨ 02ｨｰ 06 ﾂ02ｨｪｨｹ 09050603ｨｪ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0806ｨｦ, 0202 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 06ｨｰ 06ｨ 08ｨ 0402ｨ ｨｺ 06ｨ 08ｨ 0402ｨｮ ｨｪ02 0909020001ｨ 06 ﾂ0209ｨｨ01ｨｪ04, 070605ｨｰ06ｨｬｨｮ 090604ｨｪｨｨｨｺ05ｨ ｨｪ0206ｨ 010601ｨｨｨｬ0609ｨｰｨｹ ｨｮ070608070106- ﾂｨｨｨｰｨｹ 070605ｨｮ ﾂ02ｨｪｨｪ04ｨｦ ｨｬｨ ｨｰ0208ｨｨｨ 05, ﾂｨｰ06ｨ 04 09040709ｨｨｨｰｨｹ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ04, ｨｪｨ ｨｨｨ 06050202 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 0607080201020502ｨｪｨｪ060006 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ. 00 05ｨｰ06ｨｬ ｨｨ09090502010609ｨ ｨｪｨｨｨｨ ｨｬ04 080203ｨｨ05ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｰｨｹ ｨｬ02ｨｰ0601 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03, 0708ｨｨｨｪ02ｨｨ07 ｨｺ06ｨｰ0608060006 ｨ 0405 0607ｨｨ09ｨ ｨｪ 09040302. 0609070605ｨｹ040609ｨ ｨｪｨｪ04ｨｦ 070601010601 070604090605ｨｨ05 ｨｪｨ ｨｬ 0904010205ｨｨｨｰｨｹ 0008ｨｮ070704 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, 0706010902080302ｨｪｨｪ0402 ｨｨ05ｨｨ ｨｪ02 0706010902080302ｨｪｨｪ0402 0905ｨｨ07ｨｪｨｨ06 0904ｨ 08ｨ ｨｪｨｪ0401 ｨｪｨ ｨｬｨｨ 05ｨｺ06050600ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609 (ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｹ 0706 ﾂ0904, ｨ 000806ｨｰ0201ｨｪｨｨｨｺｨ ｨｨ 0906040102050409ｨ 02ｨｬｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ ). 06ｨｪｨｰ02080209ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ, ﾂｨｰ06 09 ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬ 06ｨｰｨｪ060302ｨｪｨｨｨｨ 0008ｨｮ0707ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ010907 070601 0905ｨｨ07ｨｪｨｨ02ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ0401 00ｨ ｨｺｨｰ06080609, 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 0708ｨｨｨｬ0208ｨｪ06 08ｨ 09ｨｪｨ 0008ｨｮ070702 ｨｪ02 ｨｨ04ｨｬ02ｨｪ0706ｨｴｨｨ010907 070601 0906040102ｨｦ09ｨｰ09ｨｨ02ｨｬ 05ｨｰｨｨ01 00ｨ ｨｺｨｰ06080609 (08ｨｨ09. 2). 0201ｨｪｨ ｨｺ06 05ｨｰｨｨ 0008ｨｮ070704 09ｨｨ05ｨｹｨｪ06 08ｨ 0405ｨｨ ﾂｨ 05ｨｨ09ｨｹ 0706 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ06ｨｬｨｮ 090609ｨｰｨ 09ｨｮ: 0008ｨｮ0707ｨ, 0706010902080302ｨｪｨｪｨ 07 0905ｨｨ07ｨｪｨｨ06, ｨ 0405ｨ ｨ 06050202 0601ｨｪ06080601ｨｪｨ. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｬ0603ｨｪ06 ｨｮｨｰ0902080301ｨ ｨｰｨｹ, ﾂｨｰ06 0906040102ｨｦ09ｨｰ09ｨｨ06 07060109020800ｨ 02ｨｰ0907 ｨｪ02 06 ﾂ02ｨｪｨｹ ｨ 0605ｨｹ03ｨ 07 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨ ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 0108ｨｮ00ｨｨ01 0609ｨｰｨ 02ｨｰ0907 ｨ 06050202 ｨｨ05ｨｨ ｨｬ02ｨｪ0202 89
1309ｨｰｨ ｨ ｨｨ05ｨｹｨｪ06ｨｦ. 04ｨｨ09. 2. 02060507 (%) 09ｨｨ010609 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 09 06ｨ 08ｨ 0402ｨ 01 0706 ﾂ0904, ｨｪ02 0706010902080302ｨｪｨｪ0401 (ｨ ) ｨｨ 0706010902080302ｨｪｨｪ0401 (ｨ ) 0905ｨｨ07ｨｪｨｨ06 ｨｨ0909050201ｨｮ02ｨｬ0401 00ｨ ｨｺｨｰ06080609 (0706 01ｨ ｨｪｨｪ04ｨｬ 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S-08040108): I, II ｨｨ III ｪ 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02, 0902090606ｨ 060806ｨｰ ｨｨ 0906040102050409ｨ 02ｨｬｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ 070605020906ｨｦ 060704ｨｰ 04060909ｨｨｨｦ09ｨｺ060006 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 ｨ 0008ｨ 08ｨｪ060006 ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ 00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ, 00. 000609ｨｺ09ｨ, ｨｨ06ｨｪｨｹ 2010 000601ｨ ). 0308020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ ｨｰ080201 00ｨｨ05 ｪ Acidobacteria, Actinobacteria, Proteobacteria ｨ 0405ｨｨ ｨｬｨｪ060006 ﾂｨｨ090502ｨｪｨｪ04 0906 09090201 06ｨ 08ｨ 0402ｨ 01 ｨｨ 0706010902080302ｨｪ04 0905ｨｨ07ｨｪｨｨ06 09090201 ｨｨ0909050201ｨｮ02ｨｬ0401 00ｨ ｨｺｨｰ06080609. 06ｨｪｨｰ02080209ｨｪ06 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ 00ｨｨ05ｨｮ Gemmatimonadetes (10): 0901060107ｨｴｨｨｨｦ 09 ｨｪ0202 09ｨｨ01 Gemmatimonas sp. 06ｨｺｨ 04ｨ 050907 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04ｨｬ ｨｺ 0906040102ｨｦ09ｨｰ09ｨｨ06 09090201 00ｨ ｨｺｨｰ06080609. 030905ｨｨ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ 0905ｨｨ07ｨｪｨｨ02 05ｨｺ06050600ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609 ｨｪｨ 08060104 ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｬ0603ｨｪ06 09040709ｨｨｨｰｨｹ 080701 ｨｨｨｪｨｰ02080209ｨｪ0401 04ｨ ｨｺ06ｨｪ06ｨｬ0208ｨｪ0609ｨｰ02ｨｦ (08ｨｨ09. 3). 06ｨ ｨｺ, Rizobium sp. ｨｨ Gemmatimonas sp. ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04 ｨｺ06 090902ｨｬ ｨｰ0802ｨｬ ｨｨ04ｨｮ ﾂ02ｨｪｨｪ04ｨｬ ｨｪｨ ｨｬｨｨ 00ｨ ｨｺｨｰ0608ｨ ｨｬ (ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02, 0902090606ｨ 060806ｨｰ, ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ 08ｨ 09ｨｰ02ｨｪｨｨｨｦ). 0005ｨｨ07ｨｪｨｨ06 09090201 ｨｰ080201 00ｨ ｨｺｨｰ06080609 0706010902080302ｨｪ04 ｨｰｨ ｨｺ0302 08ｨ 0405ｨｨ ﾂｨｪ0402 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ 00ｨｨ0504 Acidobacteria. 010209ｨｬ06ｨｰ0807 ｨｪｨ ｨｰ06, ﾂｨｰ06 070609050201ｨｪｨｨ02, ｨｺｨ ｨｺ ｨｮ0302 06ｨｰｨｬ02 ﾂｨ 050609ｨｹ, 070802010706 ﾂｨｨｨｰｨ 06ｨｰ ｨ 06050202 ｨｺｨｨ0905ｨｮ06 09080201ｨｮ 06ｨ ｨｨｨｰｨ ｨｪｨｨ07 (9), 09ｨｨ01ｨｪ06, ﾂｨｰ06 ｨｪ02 090902 0708020109ｨｰｨ 09ｨｨｨｰ0205ｨｨ 05ｨｰ06ｨｦ 00ｨｨ0504 0708060709ｨｨ05ｨｨ ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0609ｨｰｨｹ ｨｺ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ06 ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｨ 0706 ﾂ0904. 060605ｨｹｨｺ06 ｨｮ 0806010609 Gp1 ｨｨ Gp2 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ 09 06ｨ 08ｨ 0402ｨ 01 09 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ02ｨｬ ｨｮｨｬ02ｨｪｨｹ03ｨ 05ｨ 09ｨｹ, 0609ｨｰｨ 05ｨｹｨｪ0402 ｨ 02ｨｨ0106ｨ ｨ ｨｺｨｰ0208ｨｨｨｨ 06ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｮ09ｨｰ06ｨｦ ﾂｨｨ0904ｨｬｨｨ ｨｺ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ06 0801. 080806ｨｬ02 ｨｰ060006, 09ｨｰ06ｨｨｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ Sphingomonas sp., Streptomyces sp. ｨｨ Nocardioides sp., ｨｪｨ ｨｺ06ｨｰ06080402 0905ｨｨ0705ｨｨ 05ｨｨ03ｨｹ 00ｨ ｨｺｨｰ060804, 09090704ｨ ｨｪｨｪ0402 09 0906040102050409ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0806ｨｦ ｨｨ 0902090606ｨ 060806ｨｰ06ｨｬ. 0604ｨｬ02ｨｪ02ｨｪｨｨ02 0801 ｨｪ0204ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 09ｨｺｨ 04ｨ 05ｨｨ09ｨｹ ｨｪｨ 05ｨｰｨｨ01 09ｨｨ01ｨ 01 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609. Arthrobacter sp. ｨ 0405 ﾂｨｮ0909ｨｰ09ｨｨｨｰ020502ｨｪ ｨｺ 0906040102ｨｦ09ｨｰ09ｨｨ06 0902090606ｨ 060806ｨｰｨ ｨｨ 090408ｨ ｨｴｨｨ09ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0802. 0604090209ｨｰｨｺ0609ｨ ｨｪｨｨ02 09ｨｪｨｨ03ｨ 0506 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｹ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ ｨｨ04 0806010609 Solirubrobacter, Paenibacillus, Bradyrhizobium. 04ｨｨ09. 3. 04060104 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, 0706010902080302ｨｪｨｪ0401 0905ｨｨ07ｨｪｨｨ06 ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ07, 0902090606ｨ 060806ｨｰｨ ｨｨ 0906040102050409ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804 (ｨｰ0802ｨｮ000605ｨｹｨｪｨｨｨｺｨｨ ｪ 080603ｨｹ, ﾂ0208ｨｪ0402 ｨｺ08ｨｮ03ｨｺｨｨ ｪ 0502ｨｪ), ｨｺ06ｨｰ06080402 ｨ 0405ｨｨ 090407090502ｨｪ04 09 ｨｨ09090502010609ｨ ｨｪｨｪ0401 06ｨ 08ｨ 0402ｨ 01 0706 ﾂ0904 0706 01ｨ ｨｪｨｪ04ｨｬ 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 0902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 ｨ ｨｨｨ 05ｨｨ06ｨｰ02ｨｺ 0002ｨｪｨ 16S-08040108. 030807ｨｬ06ｨｮ000605ｨｹｨｪｨｨｨｺｨ ｨｬｨｨ 06ｨｰｨｬ02 ﾂ02ｨｪ04 08060104, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ06 090902ｨｬ ｨｰ0802ｨｬ 00ｨ ｨｺｨｰ0608ｨ ｨｬ (09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ04ｨｦ 070605020906ｨｦ 060704ｨｰ 04060909ｨｨｨｦ09ｨｺ060006 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 ｨ 0008ｨ 08ｨｪ060006 ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ 00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ, 00. 000609ｨｺ09ｨ, ｨｨ06ｨｪｨｹ 2010 000601ｨ ). 0005ｨｨ07ｨｪｨｨ06 0902090606ｨ 060806ｨｰｨ 06ｨｺｨ 04ｨ 05ｨ 09ｨｹ 0706010902080302ｨｪｨ 010609ｨｰｨ ｨｰ06 ﾂｨｪ06 06ｨ 03ｨｨ08ｨｪｨ 07 0008ｨｮ0707ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, 09080201ｨｨ ｨｺ06ｨｰ06080401 Nocardioides sp., Spartobacteria sp., Hyphomicrobium sp., Streptomyces sp. ｨｨ 0108. 050205ｨｹ09ｨｺ0601060407ｨｦ09ｨｰ0902ｨｪｨｪｨ 07 ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ (09 ｨｪｨ 0302ｨｬ 0905ｨｮ ﾂｨ 02 0502ｨｪ ｨｨ 0604ｨｨｨｬｨ 07 080603ｨｹ) 0905ｨｨ0702ｨｰ ｨｪｨ Nocardioides sp., Sphi- 90
13ngomonas sp., Streptomyces sp. ｨｨ 0108. 0201ｨｪｨ ｨｺ06 090205ｨｨｨｺ 0907ｨｨ0906ｨｺ ｨｨ ｨｰ0201 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｺ06ｨｰ06080402 ｨｪ02 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ04 ｨｺ ｨｮｨｺｨ 04ｨ ｨｪｨｪ06ｨｬｨｮ 00ｨ ｨｺｨｰ0608ｨｮ. 05080201ｨｨ ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090401 ｨｺ 0906040102ｨｦ09ｨｰ09ｨｨ06 09090201 ｨｰ080201 00ｨ ｨｺｨｰ06080609, ｨｬ0603ｨｪ06 0904010205ｨｨｨｰｨｹ ｨｰｨ ｨｺｨｨ01 ｨｨ04090209ｨｰｨｪ0401 0708020109ｨｰｨ 09ｨｨｨｰ020502ｨｦ 0706 ﾂ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｺｨ ｨｺ Pseudomonas sp., Bacillus sp., Rubrobacter sp. 06ｨ ｨｺｨｨｨｬ 06ｨ 08ｨ 0406ｨｬ, ｨｨ09090502010609ｨ ｨｪｨｪ0402 ｨｪｨ ｨｬｨｨ 00ｨ ｨｺｨｰ060804 06ｨｺｨ 040409ｨ 05ｨｨ 09ｨｨ05ｨｹｨｪ0602 0906040102ｨｦ09ｨｰ09ｨｨ02 ｨｪｨ 090609ｨｰｨ 09 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ, 01ｨ 09ｨ 07 070802ｨｨｨｬｨｮｨｴ0209ｨｰ0906 09 08ｨ 0409ｨｨｨｰｨｨｨｨ 0607080201020502ｨｪｨｪ04ｨｬ 0008ｨｮ0707ｨ ｨｬ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｨｨｬ0206ｨｴｨｨ01 ｨｰ02 00ｨｨ04ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ02 060906ｨ 02ｨｪｨｪ0609ｨｰｨｨ, ｨｺ06ｨｰ06080402 0706040906050706ｨｰ ｨｨｨｬ 04ｨ ｨｪｨｨｨｬｨ ｨｰｨｹ 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪｨｮ06 ﾂｨ 09ｨｰｨｹ 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ06ｨｦ ｨｪｨｨ03ｨｨ. 05ｨｮｨｴ0209ｨｰ09ｨｮ02ｨｰ 0007060109ｨｨ03ｨｪｨ 0703 ﾂｨ 09ｨｰｨｹ ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 0706 ﾂ0904, 09 ｨｪ0202 0901060107ｨｰ ｨｪｨ ｨｨｨ 06050202 ｨｬｨｪ060006 ﾂｨｨ090502ｨｪｨｪ0402 08060104 0706 ﾂ0902ｨｪｨｪ0401 ｨ ｨ ｨｺｨｰ0208ｨｨｨｦ, ｨｰｨ ｨｺｨｨ02 ｨｺｨ ｨｺ Rhizobium, Arthrobacter (09ｨｬ. 08ｨｨ09. 3). 06ｨｰｨ ｨ ｨｺｨｰｨｨ09ｨｪｨ 07 0008ｨｮ0707ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ 04ｨ 0102ｨｦ09ｨｰ090609ｨ ｨｪｨ 09 0609ｨｪ0609ｨｪ0401 0706 ﾂ090606ｨ 08ｨ 040609ｨ ｨｰ0205ｨｹｨｪ0401 07080602020909ｨ 01 ｨｨ 070605ｨｰ06ｨｬｨｮ ｨ 06050202 ﾂｨｮｨｰｨｺ06 0802ｨ 00ｨｨ08ｨｮ02ｨｰ ｨｪｨ 09ｨｬ02ｨｪｨｮ ｨｮ09050609ｨｨｨｦ 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ 0908020104. 00 ｨｰ06 0302 090802ｨｬ07 09 0706 ﾂ0902 070609ｨｰ0607ｨｪｨｪ06 09060108ｨ ｨｪ0702ｨｰ0907 07ｨｮ05 09090201 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ｨｺ06ｨｰ060804ｨｦ 0905ｨｮ03ｨｨｨｰ ｨｨ09ｨｰ06 ﾂｨｪｨｨｨｺ06ｨｬ 070609ｨｰ0607ｨｪｨｪ060006 ｨｺｨ ﾂ0209ｨｰ0902ｨｪｨｪ060006 ｨｨ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ060006 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 090609ｨｰｨ 09ｨ ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ. 010209ｨｬ06ｨｰ0807 ｨｪｨ 06ｨ ｨｪｨ 08ｨｮ0302ｨｪｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 0706 ﾂ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06ｨｰ04, ｨｬ0603ｨｪ06 09ｨｺｨ 04ｨ ｨｰｨｹ, ﾂｨｰ06 05ｨｰ06 090606ｨ ｨｴ0209ｨｰ0906, ｨｮ09ｨｰ06ｨｦ ﾂｨｨ090602 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 ｨｬｨｪ0600ｨｨ01 09ｨｪ0203ｨｪｨｨ01 00ｨ ｨｺｨｰ06080609 (ｨｺｨｨ090506ｨｰｨｪ0609ｨｰｨｹ, ｨｮ09050609ｨｨ07, 09060401ｨ 09ｨ 02ｨｬ0402 090905020109ｨｰ09ｨｨ02 0708ｨｨｨｬ02ｨｪ02ｨｪｨｨ07 ｨ 000806ｨｰ0201ｨｪｨｨｨｺｨｨ). 00 04ｨ ｨｺ0506 ﾂ02ｨｪｨｨ02 06ｨｰｨｬ02ｨｰｨｨｨｬ, ﾂｨｰ06 09040709ｨｨｨｰｨｹ 0008ｨｮ070704 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0402 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 04ｨ 01ｨ ｨｪｨｪ060006 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ, ｨｮ01ｨ 02ｨｰ0907 ｨｪ02 0909020001ｨ. 030605ｨｰ06ｨｬｨｮ ｨｪｨ ｨｬｨｨ ｨ 0405 06070806ｨ 0609ｨ ｨｪ ｨｬ02ｨｰ0601 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03, 0706040906050706ｨｴｨｨｨｦ ｨ 06050202 0005ｨｮｨ 06ｨｺ06 ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ ｨｰｨｹ ｨｨ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨｨ080609ｨ ｨｰｨｹ 01ｨ ｨｪｨｪ0402 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 0706 ﾂ0902ｨｪｨｪ06ｨｦ 020108. 02ｨｪ 070604090605ｨｨ05 09090704ｨ ｨｰｨｹ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ﾂｨｨ090502ｨｪｨｪ0609ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0401 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 09 0905ｨｨ07ｨｪｨｨ02ｨｬ 05ｨｺ06050600ｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609. 0602ｨｬ ｨｪ02 ｨｬ02ｨｪ0202, ｨｨｨｪ060001ｨ ｨｪ020706ｨｪ07ｨｰｨｪ06, 0706 ﾂ02ｨｬｨｮ ｨｰｨ ｨｨ05ｨｨ ｨｨｨｪｨ 07 0008ｨｮ0707ｨ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪｨ 05ｨｨｨ 06 ｨｪ02 ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪｨ ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ, 070609ｨｺ0605ｨｹｨｺｨｮ 010507 060202ｨｪｨｺｨｨ 05ｨｰ060006 ｨｰ0802ｨ ｨｮ02ｨｰ0907 04ｨｪｨ ｨｪｨｨ02 00ｨｨ04ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 060906ｨ 02ｨｪｨｪ0609ｨｰ02ｨｦ ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609. 0609090502010609ｨ ｨｰｨｹ 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪｨｮ06 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｮ 09050603ｨｪ0202, ﾂ02ｨｬ ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺｨｮ06, 070605ｨｰ06ｨｬｨｮ ｨｪ0206ｨ 010601ｨｨｨｬ06 060008ｨ ｨｪｨｨ ﾂｨｨｨｰｨｹ ｨｺ08ｨｮ00 ｨｨ04ｨｮ ﾂｨ 02ｨｬ0401 ｨｬｨｨｨｺ0806060800ｨ ｨｪｨｨ04ｨｬ0609 ｨｨ 08ｨ 0909ｨｬｨ ｨｰ08ｨｨ09ｨ ｨｰｨｹ ｨｰ0605ｨｹｨｺ06 ｨｰ02 ｨｨ04 ｨｪｨｨ01, ｨｺ06ｨｰ06080402 0802ｨ 00ｨｨ08ｨｮ06ｨｰ ｨｪｨ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ02 00ｨ ｨｺｨｰ06080609 06ｨｺ08ｨｮ03ｨ 06ｨｴ02ｨｦ 0908020104. 0002ｨｰ0601ｨｨｨｺｨ 07080609020102ｨｪｨｪ060006 ｨｪｨ ｨｬｨｨ ｨｨ09090502010609ｨ ｨｪｨｨ07 0708020109ｨｰｨ 09050702ｨｰ 0906ｨ 06ｨｦ 0702080904ｨｦ 03ｨ 00 09 05ｨｰ06ｨｬ ｨｪｨ 0708ｨ 090502ｨｪｨｨｨｨ, ｨｰｨ ｨｺ ｨｺｨ ｨｺ 01ｨ 02ｨｰ 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 090606ｨｰｨｪ0209ｨｰｨｨ ｨｺ06ｨｪｨｺ0802ｨｰｨｪ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 090606ｨ ｨｴ0209ｨｰ09ｨ 09 0102ｨｦ09ｨｰ09ｨｨ02ｨｬ 0607080201020502ｨｪｨｪ060006 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ060006 00ｨ ｨｺｨｰ0608ｨ. 06ｨｰｨ ｨｺ, ｨｪｨ 0609ｨｪ060902 ｨ ｨｪｨ 05ｨｨ04ｨ 01ｨ ｨｪｨｪ0401 09040906ｨｺ06070806ｨｨ04090601ｨｨｨｰ0205ｨｹｨｪ060006 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 07080609020102ｨｪ 0706010806ｨ ｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0804 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 09 09ｨｨ09ｨｰ02ｨｬ02 ｨｬｨｪ0600060502ｨｰｨｪ020006 09ｨｰｨ 02ｨｨ06ｨｪｨ 08ｨｪ060006 0706050209060006 060704ｨｰｨ 04060909ｨｨｨｦ09ｨｺ060006 000609ｨｮ01ｨ 0809ｨｰ0902ｨｪｨｪ060006 ｨ 0008ｨ 08ｨｪ060006 ｨｮｨｪｨｨ09020809ｨｨｨｰ02ｨｰｨ ｪ00050908 ｨｨｨｬ. 08.08. 06ｨｨｨｬｨｨ0807040209ｨ (09 090609ｨｹｨｬｨｨ 09ｨ 08ｨｨｨ ｨｪｨｰｨ 01, 06ｨｰ08ｨ 03ｨ 06ｨｴｨｨ01 05ｨｺ06050600ｨｨ ﾂ0209ｨｺ0602 0905ｨｨ07ｨｪｨｨ02 08ｨ 0405ｨｨ ﾂｨｪ0401 0906 ﾂ02ｨｰｨ ｨｪｨｨｨｦ ｨｰ080201 ｨ 000806ｨｰ0201ｨｪｨｨ ﾂ0209ｨｺｨｨ01 00ｨ ｨｺｨｰ06080609 ｪ 0906040102050409ｨ 02ｨｬ06ｨｦ ｨｺｨｮ05ｨｹｨｰｨｮ0804, 0902090606ｨ 060806ｨｰｨ ｨｨ ｨｨ04090209ｨｰｨｺ0609ｨ ｨｪｨｨ07 0706 ﾂ0904). 0308ｨｨ ｨ ｨｪｨ 05ｨｨ0402 ｨｨ 09ｨｨ09ｨｰ02ｨｬｨ ｨｰｨｨ04ｨ 02ｨｨｨｨ 080204ｨｮ05ｨｹｨｰｨ ｨｰ0609 07ｨｨ08060902ｨｺ0902ｨｪｨｨ080609ｨ ｨｪｨｨ07 0706 ﾂ0902ｨｪｨｪ06ｨｦ 020108 ｨ 0405 06070806ｨ 0609ｨ ｨｪ ｨｬ02ｨｰ0601 00ｨ ｨｨｨｪｨ 08ｨｪ060006 0905ｨｬ0705ｨｨｨｪ00ｨ 03, ﾂｨｰ06 01ｨ 0506 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 09040709ｨｨｨｰｨｹ 0008ｨｮ070704 060800ｨ ｨｪｨｨ04ｨｬ0609, ﾂｨｮ0909ｨｰ09ｨｨｨｰ0205ｨｹｨｪ0401 ｨｺ 0102ｨｦ09ｨｰ09ｨｨ06 04ｨ 01ｨ ｨｪｨｪ0401 00ｨ ｨｺｨｰ06080609. 0306ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 0708020105ｨ 00ｨ 02ｨｬ04ｨｦ 070601010601 0706040906050702ｨｰ 090606ｨｰｨｪ0209ｨｰｨｨ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 09 ｨｰｨ ｨｺ0906ｨｪ06ｨｬｨｨ ﾂ0209ｨｺ06ｨｦ 09ｨｰ08ｨｮｨｺｨｰｨｮ0802 ｨｬｨｨｨｺ0806ｨ ｨｪ060006 090606ｨ ｨｴ0209ｨｰ09ｨ 09 0607080201020502ｨｪｨｪ04ｨｬ 09ｨｪ0203ｨｪｨｨｨｬ 0906040102ｨｦ09ｨｰ09ｨｨ02ｨｬ. 010107 00090208060909ｨｨｨｦ09ｨｺｨｨｨｦ 010606 090205ｨｹ09ｨｺ0601060407ｨｦ09ｨｰ0902ｨｪｨｪ06ｨｦ ｨｬｨｨｨｺ0806ｨ ｨｨ06050600ｨｨｨｨ 040609090205ｨｹ010604ｨ ｨｺｨ 0102ｨｬｨｨｨｨ, 196608 04060909ｨｨ07, 00. 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800 ｪ03ｨｮ03ｨｺｨｨｨｪ, 03. 030601ｨ 0205ｨｹ09ｨｺ060006, 3, e-mail: doumova@gmail.com 030609ｨｰｨｮ07ｨｨ05ｨ 09 080201ｨ ｨｺ02ｨｨ06 28 ｨｨ06ｨｪ07 2012 000601ｨ 91
13Sel ｯskokhozyaistvennaya biologiya [Agricultural Biology], 2013, 01 5, pp. 85-92 THE MAIN TRENDS IN DYNAMICS OF SOIL MICROBIOM DURING A LONG-TERM FIELD EXPERIMENT AS INDICATED BY HIGH THROUGHPUT SEQUENCING THE 16S-rRNA GENE LIBRARIES 92 V.A. Dumova, E.V. Pershina, Ya.V. Merzlyakova, Yu.V. Kruglov, E.E. Andronov All-Russian Research Institute of Agricultural Microbiology, Russian Academy of Agricultural Sciences, 3, sh. Podbelskogo, Saint Petersburg-Pushkin, 196608 Russia, e-mail doumova@gmail.com Received June 28, 2012 doi: 10.15389/agrobiology.2013.5.85eng Acknowledgements: Supported by Ministry of Education and Science of the Russian Federation, and Russian Foundation for Basic Research Abstract A biodiversity of soil microbial communities now is being widely studied. Since 1912, the influence of different agrotechnical factors on soil is being examined in the stationary field experi-ment in the Moscow Agricultural Academy. We investigated an effect of different combination of three factors, i.e. plants (flax and winter rye), crop rotation and soil acidity, to the soil microbiom. To estimate a biodiversity, the throughput sequencing was used. Also an original approach, the bi-nary sampling, was suggested to analyze the data of throughput sequencing. It was shown the number of microorganisms which changed under the influence of studied factor is approximately equal to those staying unchanged. But a taxonomic composition of these two groups differed, and the impacted group was more uniform. Thus, we can conclude that the less part of microorganism is sensitive to the acting factors, and the rest ones keep their number unchanged, more or less. The taxonomic groups of microorganisms, sensitive to each of studied factors, were revealed. The binary sampling, suggested herein, allows finding out a relation between an action of some ecological fac-tor and varying taxonomic structure of microbial population. Keywords: soil microbiom, high throughput sequencing. REFERENCES 1. Pace N.R. Mapping the tree of life: progress and prospects. Microbiology and Molecular Biology Reviews, 2009, 73(4): 565-576. 2. Tyson G.W., Banfield J.F. Cultivating the uncultivated: a community genomics perspective. Trends in Microbiology, 2005, 9(13): 411-415. 3. Kakirde K.S., Parsley L.C., Liles M.R. Size does matter: application-driven approaches for soil metagenomics. Soil Biol. Biochem., 2010, 42: 1911-1923. 4. Lombard N., Prestat E., Elsas J.D.V., Simonet P. Soil-specific limitations for access and analysis of soil microbial communities by metagenomics. FEMS Microbiol. Ecol., 2011, 78: 31-49. 5. Wooley J.C., Ye Y. Metagenomics: Facts and artifacts, and computational challenges. Journal of Computer Science and Technology, 2010, 1(25): 71-81. 6. Mardis E.R. Next-generation DNA sequencing methods. Annual Review of Genomics and Human Genetics, 2008, 9: 211-219. 7. Bates S.T.D., Berg-Lyons J.G., Caporaso W.A. et al. Examining the global distribution of dominant archaeal populations in soil. ISME Journal, 2010, 5: 908-917. 8. Cole J.R., Wang Q., Cardenas E. et al. The Ribosomal Database Project: improved alignments and new tools for rrna analysis. Nucl. Acids Res., 2009, 37: D141-D145. 9. Lauber C.L., Strickland M.S., Bradford M.A., Fierer N. The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biol. Biochem., 2008, 40: 2407-2415. 10. Zhang H., Sekiguchi Y., Hanada S. et al. Gemmatimonas aurantiaca gen. nov., sp. nov., a gramnegative, aerobic, polyphosphate-accumulating micro-organism, the first cultured representative of the new bacterial phylum Gemmatimonadetes phyl. nov. International Journal of Systematic and Evolutionary Microbioly, 2003, 53(4): 1155-1163. 01ｨ ｨｮ ﾂｨｪ0402 0906ｨ 08ｨ ｨｪｨｨ07 THE 2 nd PLANT GENOMICS CONGRESS (12-13 May, 2014, London, UK) Attracting experts working in next generation sequencing, plant genomics/sciences, epigenetics, bioinformatics and data management, the conference will continue to examine the latest NGS platforms and technologies suitable for progressing plant based research as well as tools to enable successful analysis. 06ｨｪ000608ｨｬｨ 02ｨｨ07: http://www.globalengage.co.uk