Tsitologiya 54(11)

132012 000606020902010608 0606ｨｬ 54, 0111 05040800010606030905010407 080108090605 090804080806030408 080907020403050003010601020102 0003 ﾁ03010608 08020304 040801010609 060009040602010200 00040106 030406 06050302090505020008010606 08010606060309 08 0408050906 ﾁ010400 07 ﾁ080506080800 0002090308070904 080806060108 08 06. 02. 090600060506ｨ 0609ｨ 06ｨｪ09ｨｰｨｨｨｰｨｮｨｰ 02ｨｨｨｰ06050600ｨｨｨｨ 040801, 05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800; 050502ｨｺｨｰ0806ｨｪｨｪ04ｨｦ ｨ 01080209: ibogol@mail.ru 06. 02. 090600060506ｨ 0609ｨ 0508ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 01ｨ 08ｨ ｨｺｨｰ0208ｨ 0005ｨｮ060802090202ｨｪｨｰｨｪ060006 ｨｬ02 ﾂ02ｨｪｨｨ07 07010208 08ｨ ｨｪｨｪｨｨ01 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 0308020109ｨｰｨ 090502ｨｪ04 ｨｪ06090402 01ｨ ｨｪｨｪ0402 06ｨ 060906ｨ 02ｨｪｨｪ0609ｨｰ0701 ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ060802090202ｨｪｨｰｨｪ060006 090407090502ｨｪｨｨ07 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ 09 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪｨ 01 ｨｬ0403ｨｨ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ 0005ｨｮ060802090202ｨｪｨｰｨｪ06ｨｬ02 ﾂ02ｨｪｨｪ0401 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05- ｨｨ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504. 0604ｨｮ ﾂ02ｨｪ06 0702080208ｨ 0907080201020502ｨｪｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 0708ｨｨ ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ06ｨｬ ｨｨｨｪ00ｨｨｨ ｨｨ080609ｨ ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ, ｨ ｨｰｨ ｨｺ0302 0706090502 000208ｨｬ02ｨｪｨｰｨ ｨｰｨｨ09ｨｪ060006 08ｨ 09ｨｴ02070502ｨｪｨｨ07 020108. 00ｨｨ04ｨｮｨ 05ｨｹｨｪ0402 ｨｪｨ ｨ 05060102ｨｪｨｨ07 0106070605ｨｪ02ｨｪ04 0908ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｬ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902ｨｪｨｪ04ｨｬ ｨ ｨｪｨ 05ｨｨ0406ｨｬ ｨｨｨｪｨｰ020008ｨ 05ｨｹｨｪ06ｨｦ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ ｨｪｨ ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ0401 ｨｨ0406ｨ 08ｨ 0302ｨｪｨｨ0701. 00 06ｨ 02ｨｨ01 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰｨ 05ｨｹｨｪ0401 0008ｨｮ0707ｨ 01 ｨｺｨ ｨｺ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨｨｨｪ00ｨｨｨ ｨｨｨｰ06080609 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ, ｨｰｨ ｨｺ ｨｨ 0706090502 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 020108ｨ 0406ｨｦ 090407090502ｨｪ06 ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 0706 0908ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ06ｨｪｨｰ08060502ｨｬ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ C-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ, ｨｪ06 ｨｪ02 ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨ ｨｺｨｰｨｨｨｪｨ. 030605ｨｮ ﾂ02ｨｪｨｪ0402 01ｨ ｨｪｨｪ0402 0706040906050706ｨｰ 070802010706050603ｨｨｨｰｨｹ, ﾂｨｰ06 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ ｨ 06050202 05000002ｨｺｨｰｨｨ09ｨｪ04 0708ｨｨ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ060802090202ｨｪｨｰｨｪ06ｨｬ 090407090502ｨｪｨｨｨｨ ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 010806ｨ 07ｨｴｨｨ010907 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ. 080506 ﾂ02090402 09050609ｨ : 0106ｨｨｨｬ0705ｨ ｨｪｨｰｨ 02ｨｨ06ｨｪｨｪ0402 04ｨ 0806010403ｨｨ ｨｬ0403ｨｨ, 07010208ｨｪ04ｨｦ ｨ ｨｺｨｰｨｨｨｪ, ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ07, 05ｨ 040208ｨｪｨ 07 09ｨｺｨ ｨｪｨｨ08ｨｮ06ｨｴｨ 07 ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪｨ 07 ｨｬｨｨｨｺ080609ｨｺ0607ｨｨ07. 010209ｨｬ06ｨｰ0807 ｨｪｨ ｨｰ06 ﾂｨｰ06 070208090402 090606ｨ ｨｴ02ｨｪｨｨ07 06 090407090502ｨｪｨｨｨｨ 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ (Clark, Rosenbaum, 1979; Nakayasu, Ueda, 1983; 03ｨ 080002ｨｪ0609, 01ｨ 05ｨ ｨｺｨｰｨｨ06ｨｪ0609, 1987) ｨ 0405ｨｨ 0909ｨｰ0802 ﾂ02ｨｪ04 010609ｨｰｨ ｨｰ06 ﾂｨｪ06 09ｨｺ0207ｨｰｨｨ ﾂ0209ｨｺｨｨ, ｨｨ09090502010609ｨ ｨｪｨｨ07 070609050201ｨｪｨｨ01 01020907ｨｰｨｨ0502ｨｰｨｨｨｦ 070601ｨｰ09020801ｨｨ05ｨｨ 0708ｨｨ09ｨｮｨｰ09ｨｰ09ｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 ｨｺ0502ｨｰ06 ﾂｨｪ06ｨｬ 07010802. 0906050202 ｨｰ060006, 0708060102ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ ｨｪ06 ｨｮ ﾂｨ 09ｨｰｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 02020506ｨｬ 08070102 07010208ｨｪ0401 00ｨｮｨｪｨｺ02ｨｨｨｦ, 09ｨｺ0506 ﾂｨ 07 080200ｨｮ050702ｨｨ06 0609ｨｪ0609ｨｪ0401 05ｨｰｨ 070609 05ｨｺ090708020909ｨｨｨｨ 0002ｨｪ0609 (09ｨｬ. 06ｨ 04060804: Hofmann, 2009; Visa, Percipalle, 2010). 08 090603ｨ 0502ｨｪｨｨ06, 0909020102ｨｪｨｨ07 06 ｨｬ0209ｨｰ02 ｨｨ 080605ｨｨ ｨ ｨｺｨｰｨｨｨｪｨ 09 07080609ｨｰ08ｨ ｨｪ09ｨｰ0902ｨｪｨｪ06ｨｦ 060800ｨ ｨｪｨｨ04ｨ 02ｨｨｨｨ ｨｺ0502ｨｰ06 ﾂｨｪ060006 070108ｨ 0609ｨｰｨ 06ｨｰ0907 ｨｪ02ｨｬｨｪ060006 ﾂｨｨ090502ｨｪｨｪ04ｨｬｨｨ. 02ｨ ｨｲ0709ｨｪ0702ｨｰ0907 05ｨｰ06 ｨｰ02ｨｬ, ﾂｨｰ06 ｨ 0605ｨｹ03ｨｨｨｪ09ｨｰ0906 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰｨ 05ｨｹｨｪ0401 01ｨ ｨｪｨｪ0401 06 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ06ｨｦ 080605ｨｨ 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ 070605ｨｮ ﾂ02ｨｪ06 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ01 ｨｨ ｨｬ060502ｨｺｨｮ050708ｨｪ06-ｨ ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ02ｨｰ0601ｨｨｨｺ, 04ｨ ﾂｨ 09ｨｰｨｮ06 09 ｨ 0209ｨｺ0502ｨｰ06 ﾂｨｪ0401 09ｨｨ09ｨｰ02ｨｬｨ 01, ｨｰ060001ｨ ｨｺｨ ｨｺ 09ｨｨ04ｨｮｨ 05ｨｨ04ｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 ｨｺ0502ｨｰ06 ﾂｨｪ06ｨｬ 07010802 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｺ05ｨ 0909ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ06080006050600ｨｨ ﾂ0209ｨｺｨｨ01 0706010106010609 0706 080701ｨｮ 0708ｨｨ ﾂｨｨｨｪ 04ｨ ｨｰ08ｨｮ01ｨｪ02ｨｪｨ. 030802030102 0909020006, 05ｨｰ06 09090704ｨ ｨｪ06 09 ｨｰ02ｨｬ, ﾂｨｰ06 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ ｨｬ060502ｨｺｨｮ0502 ｨ ｨｺｨｰｨｨｨｪｨ 06ｨ 05ｨ 01ｨ 06ｨｰ 09040906ｨｺ06ｨｦ ｨｨ04ｨ ｨｨ08ｨ ｨｰ0205ｨｹｨｪ0609ｨｰｨｹ06 ｨｨ, 0706-09ｨｨ01ｨｨｨｬ06ｨｬｨｮ, 09040709050706ｨｰ 08ｨ 0405ｨｨ ﾂｨｪ0402 000608ｨｬ04 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ (Milankov, De Boni, 1993; Gonsior et al., 1999). 0308ｨｨ 05ｨｰ06ｨｬ 01ｨ ｨｪｨｪｨ 07 ｨｨ04ｨ ｨｨ08ｨ ｨｰ0205ｨｹｨｪ0609ｨｰｨｹ ｨｪｨ ｨ 050601ｨ 02ｨｰ0907 ｨｰ0605ｨｹｨｺ06 09 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰｨ 01 09 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ02ｨｬ ｨｪ02070807ｨｬ06ｨｦ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ060802090202ｨｪ02ｨｨｨｨ, ｨｰ060001ｨ ｨｺｨ ｨｺ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ 0108ｨｮ00ｨｨ01 ｨｬ060502ｨｺｨｮ050708ｨｪ06-ｨ ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ02ｨｰ0601ｨｨｨｺ (ｨｪｨ 0708ｨｨｨｬ0208, ｨｨｨｬｨｬｨｮｨｪ06ｨ 0506ｨｰｨｨｨｪ00ｨ ) ｨｰ02 0302 ｨ ｨｪｨｰｨｨｨｰ0205ｨ 09040709050706ｨｰ 090902 ｨｨ0406000608ｨｬ04 ｨ ｨｺｨｰｨｨｨｪｨ (Gonsior et al., 1999). 0207ｨｨ09ｨ ｨｪ04 ｨｰｨ ｨｺ0302 0905ｨｮ ﾂｨ ｨｨ, ｨｺ060001ｨ 08ｨ 04ｨｪ0402 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ ｨ ｨｺｨｰｨｨｨｪｨｮ ｨｮ09070203ｨｪ06 06ｨｺ08ｨ 03ｨｨ09ｨ 06ｨｰ ｨｺ0502ｨｰ06 ﾂｨｪ0402 070108ｨ, 0601ｨｪｨ ｨｺ06 ｨｪｨ ｨ 050601ｨ 02ｨｬ0402 07ｨ ｨｰｨｰ0208ｨｪ04 ｨｬ02 ﾂ02ｨｪｨｨ07 08ｨ 0405ｨｨ ﾂｨ 06ｨｰ0907, ｨｺｨ ｨｺ 09 0905ｨｮ- ﾂｨ 02 09 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ 2G2 ｨｨ 1C7 (Gonsior et al., 1999; Schoenenberger et al., 2005). 01ｨ ｨｨｨ 06050202 0902080607ｨｰｨｪ06ｨｦ 00ｨｨ0706ｨｰ020406ｨｦ, 06ｨ ｨｲ0709ｨｪ0706ｨｴ02ｨｦ 01ｨ ｨｪｨｪｨｮ06 09ｨｨｨｰｨｮｨ 02ｨｨ06, ｨｬ0603ｨｪ06 09 ﾂｨｨｨｰｨ ｨｰｨｹ 090604ｨｬ0603ｨｪ0609ｨｰｨｹ 09ｨｮｨｴ0209ｨｰ090609ｨ ｨｪｨｨ07 ｨｪ0209ｨｺ0605ｨｹｨｺｨｨ01 09ｨｰ08ｨｮｨｺｨｰｨｮ08ｨｪ06-00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ0401 ｨｺ06ｨｪ000608ｨｬｨ 02ｨｨｨｦ 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ (Gonsior et al., 1999; Pederson, Aebi, 2005). 04ｨ ｨｪ0202 ｨｪｨ ｨｬｨｨ ｨｰｨ ｨｺ0302 ｨ 0405ｨｨ 0708060102ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ ｨｪ04 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ0402 08ｨ 0405ｨｨ ﾂｨｨ07 09 07ｨ ｨｰｨｰ0208ｨｪｨ 01 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205, 070605ｨｮ ﾂ02ｨｪｨｪ0401 ｨｺ C- ｨｨ N-ｨｺ06ｨｪ02020904ｨｬ 0008ｨ 00ｨｬ02ｨｪｨｰｨ ｨｬ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ (090600060506ｨ 0609ｨ, 090600060506ｨ 0609ｨ, 2009). 01ｨ 0609ｨｪ0609ｨ ｨｪｨｨｨｨ 070605ｨｮ ﾂ02ｨｪｨｪ0401 08ｨ ｨｪ0202 080204ｨｮ05ｨｹｨｰｨ ｨｰ0609 ｨ 040506 09040109ｨｨｨｪｨｮｨｰ06 07080201070605060302ｨｪｨｨ02 06 ｨｰ06ｨｬ, ﾂｨｰ06 01ｨ ｨｪｨｪ0402 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｨ04ｨ ｨｨ08ｨ ｨｰ0205ｨｹｨｪ06 09040709050706ｨｰ 09 070108ｨ 01 05ｨｬｨ 08ｨｨ06ｨｪ0609 010902 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ06 08ｨ 0405ｨｨ ﾂｨｪ0402 000608ｨｬ04 0605ｨｨ0006ｨｬ0208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ. 000205ｨｹ ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ 08ｨ ｨ 06ｨｰ04 04ｨ ｨｺ0506 ﾂｨ 05ｨ 09ｨｹ 09 070605ｨｮ ﾂ02ｨｪｨｨｨｨ ｨｪ06090401 01ｨ ｨｪｨｪ0401 06 090604ｨｬ0603ｨｪ06ｨｬ 0906090502 ﾂ02ｨｪｨｨｨｨ 09040709050702ｨｬ0401 07060106ｨ ｨｪ04ｨｬ 06ｨ 08ｨ 0406ｨｬ 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ0401 000608ｨｬ 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ 09 0708060202090904 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ 07080609ｨｰ08ｨ ｨｪ09ｨｰ0902ｨｪｨｪ06ｨｦ 060800ｨ ｨｪｨｨ04ｨ 02ｨｨｨｨ 010806ｨｬｨ ｨｰｨｨｨｪｨ. 020507 05ｨｰ060006 ｨｬ04 ｨｨ04ｨｮ ﾂｨｨ05ｨｨ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ02 07ｨ ｨｰｨｰ0208ｨｪｨ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ060802090202ｨｪｨｰｨｪ060006 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ 09 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪｨ 01 ｨｬ0403ｨｨ 0708ｨｨ ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ06ｨｬ ｨｨｨｪ00ｨｨｨ ｨｨ080609ｨ ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ 0706090502 000208ｨｬ02ｨｪｨｰｨ ｨｰｨｨ09ｨｪ060006 08ｨ 09ｨｴ02070502ｨｪｨｨ07 020108. 00ｨ ｨｰ0208ｨｨｨ 05 ｨｨ ｨｬ02ｨｰ0601ｨｨｨｺｨ 030605ｨｮ ﾂ02ｨｪｨｨ02 05ｨｬｨ 08ｨｨ06ｨｪ0609. 04ｨ ｨ 06ｨｰｨ 0904070605ｨｪ02ｨｪｨ ｨｪｨ ｨｬ0403ｨ 01 BALB/c ｨｨ04 07ｨｨｨｰ06ｨｬｨｪｨｨｨｺｨ 04080001 0004ｨ 0707060506090603 (05ｨ ｨｪｨｺｨｰ-0302ｨｰ0208ｨ ｨｮ0800). 020507 070605ｨｮ ﾂ02ｨｪｨｨ07 03ｨｨ0906ｨｰｨｪ0401 09 01ｨ ｨｰｨｨ080609ｨ ｨｪｨｪ04ｨｬ 090806ｨｺ06ｨｬ ｨ 020802ｨｬ02ｨｪｨｪ0609ｨｰｨｨ ｨｮ 09ｨ ｨｬ06ｨｺ ｨｨｨｪ01ｨｮ02ｨｨ080609ｨ 05ｨｨ 0609ｨｮ050702ｨｨ06 0706090502010609ｨ ｨｰ0205ｨｹｨｪ04ｨｬｨｨ 0601ｨｪ06ｨｺ08ｨ ｨｰｨｪ04ｨｬｨｨ ｨｨｨｪｨｲ02ｨｺ02ｨｨ07ｨｬｨｨ 090409060806ｨｰ06 ﾂｨｪ060006 (Folligon, Intervet, 01060505ｨ ｨｪ01ｨｨ07) ｨｨ 010608ｨｨ06ｨｪｨｨ- 831

832 06. 02. 090600060506ｨ 0609ｨ 04ｨｨ09. 1. 0906ｨｺｨ 05ｨｨ04ｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ. 06ｨｬｨ 08ｨｨ06ｨｪ04: ｨ ｪ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0402, ｨ ｪ 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 020108ｨ 0406ｨｦ, 09 ｪ 0706090502 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 5,6-01ｨｨ01050608-1b-02-08ｨｨｨ 0600ｨｮ08ｨ ｨｪ0604ｨｨ05ｨ 02ｨｪ04ｨｨｨｬｨｨ01ｨ 04060506ｨｬ (DRB), 00 ｪ 0706090502 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪ06ｨｬ D, 01 ｪ 0706090502 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪ06ｨｬ D 09 070609050201ｨｮ06ｨｴ02ｨｦ 06ｨ 08ｨ ｨ 06ｨｰｨｺ06ｨｦ 020108ｨ 0406ｨｦ. 05ｨｰ080205ｨｺｨ ｨｬｨｨ 0706ｨｺｨ 04ｨ ｨｪ06 ｨｬ02 ﾂ02ｨｪｨｨ02 ｨｪｨ 070208ｨｨ000208ｨｨｨｨ 070806070108040302ｨｺ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｺ06ｨｰ0608060006 09060408ｨ 09ｨｰｨ 02ｨｰ 0706090502 ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ060006 070601ｨ 090502ｨｪｨｨ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｨ 09ｨｪｨｨ03ｨ 02ｨｰ0907 0706090502 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 020108ｨ 0406ｨｦ. ﾂ0209ｨｺ060006 (Chorulon, Intervet, 01060505ｨ ｨｪ01ｨｨ07) 0006ｨｪｨ 0106ｨｰ080607ｨｨｨｪ0609 0706 5 ｪ10 0003 ｨｪｨ 03ｨｨ0906ｨｰｨｪ0602 c ｨｨｨｪｨｰ020809ｨ 0506ｨｬ ｨｬ020301ｨｮ ｨｨｨｪｨｲ02ｨｺ02ｨｨ07ｨｬｨｨ 44 ｪ48 ﾂ. 00060408ｨ 09ｨｰ 04ｨ 080601040302ｨｦ 06ｨｰ09 ﾂｨｨｨｰ0409ｨ 05ｨｨ 06ｨｰ 090802ｨｬ02ｨｪｨｨ ｨｨｨｪｨｲ02ｨｺ02ｨｨｨｨ 010608ｨｨ06ｨｪｨｨ ﾂ0209ｨｺ060006 0006ｨｪｨ 0106ｨｰ080607ｨｨｨｪｨ. 06ｨｬｨ 08ｨｨ06ｨｪ04 ｨｨ04090502ｨｺｨ 05ｨｨ ｨｨ04 07ｨｦ02020906010609 ﾂ02080204 46 ﾂ 0706090502 ｨｨｨｪｨｲ02ｨｺ02ｨｨｨｨ 010608ｨｨ06ｨｪｨｨ ﾂ0209ｨｺ060006 0006ｨｪｨ 0106ｨｰ080607ｨｨｨｪｨ 09 09080201ｨｮ F10, 090601020803ｨ ｨｴｨｮ06 HEPES (25 ｨｬ00, 0801 7.2 ｪ7.4). 020507 01ｨ 05ｨｹｨｪ02ｨｦ0302ｨｦ 08ｨ ｨ 06ｨｰ04 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 04ｨ 0806010403ｨｨ, ｨｪ02 ｨｨｨｬ020903ｨｨ02 09ｨｨ01ｨｨｨｬ0401 ｨｬ06080006050600ｨｨ ﾂ0209ｨｺｨｨ01 01020002ｨｺｨｰ0609. 020507 ｨｨｨｪ00ｨｨｨ ｨｨ080609ｨ ｨｪｨｨ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ04 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ 05ｨｨ 09 05022-ｨｨｨｪｨｺｨｮｨ ｨ ｨｰ060802 09 09ｨｰｨ ｨｪ01ｨ 08ｨｰｨｪ0401 ｨｮ09050609ｨｨ0701 09 ｨｰ02 ﾂ02ｨｪｨｨ02 3 ﾂ 09 0908020102 003 (Whittingham, 1971), 090601020803ｨ ｨｴ02ｨｦ 5,6-01ｨｨ0105060806-1-b-02-08ｨｨｨ 0600ｨｮ08ｨ ｨｪ0604ｨｨ05ｨ 0204ｨｨｨｬｨｨ01ｨ 040605 (DRB; BIOMOL Research Labs) 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 500 ｨｬｨｺM, ｨｨ05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 1 ﾂ 09 0908020102 003, 090601020803ｨ ｨｴ02ｨｦ ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪ D (Sigma, 050108) 09 ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨｨｨ 5 ｨｬｨｺ00/ｨｬ05. 06 ｨｬ ｨｬ ｨｮ ｨｪ 06 00 05 ｨｮ 06 08 02 09 02 02 ｨｪ ｨｰ ｨｪ ｨ 07 ｨｬ ｨｨ ｨｺ 08 06 09 ｨｺ 06 07 ｨｨ 07. 0609070605ｨｹ040609ｨ 05ｨｨ 070605ｨｨｨｺ0506ｨｪｨ 05ｨｹｨｪ0402 ｨｺ080605ｨｨ ﾂｨｹｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ, 090408ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 070806ｨｰｨｨ09 0008ｨ 00ｨｬ02ｨｪｨｰｨ C-ｨｺ06ｨｪ020209060006 0106ｨｬ02ｨｪｨ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ (08 2066, Sigma, 050108) (08ｨ 0409020102ｨｪｨｨ02 1 : 100), 05ｨｨｨ 06 070605ｨｨｨｺ0506ｨｪｨ 05ｨｹｨｪ0402 ｨｺ080605ｨｨ ﾂｨｹｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ, 090408ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 070806ｨｰｨｨ09 0008ｨ 00ｨｬ02ｨｪｨｰｨ N-ｨｺ06ｨｪ020209060006 0106ｨｬ02ｨｪｨ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ (08 2103, Sigma, 050108) (08ｨ 0409020102ｨｪｨｨ02 1 : 200). 05070202ｨｨ00ｨｨ ﾂｨｪ0609ｨｰｨｹ 090907040409ｨ ｨｪｨｨ07 ｨ ｨｪｨｰｨｨｨｰ0205 ｨ 0405ｨ 07080609020802ｨｪｨ 09 0706ｨｬ06ｨｴｨｹ06 ｨｨｨｬｨｬｨｮｨｪ06ｨ 0506ｨｰｨｨｨｪ00ｨ. 00 ｨｺｨ ﾂ0209ｨｰ0902 09ｨｰ06080401 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ ｨｺ0604ｨｹｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ 070806ｨｰｨｨ09 ｨｨｨｬｨｬｨｮｨｪ06000506ｨ ｨｮ05ｨｨｨｪ0609 ｨｺ080605ｨｨｨｺｨ, ｨｺ06ｨｪｨｲ0600ｨｨ080609ｨ ｨｪｨｪ0402 09 FITC (Sigma, 050108). 06ｨｬｨ 08ｨｨ06ｨｪ04 00ｨｨｨｺ09ｨｨ080609ｨ 05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 40 ｪ60 ｨｬｨｨｨｪ 09 4%-ｨｪ06ｨｬ 08ｨ 09ｨｰ09060802 07ｨ 08ｨ - 000608ｨｬｨ 05ｨｹ010200ｨｨ01ｨ ｨｪｨ PBS 0708ｨｨ ｨｺ06ｨｬｨｪｨ ｨｰｨｪ06ｨｦ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802, 0706090502 ﾂ020006 06ｨｰｨｬ0409ｨ 05ｨｨ 09 ｨｰ080201 07060802ｨｨ0701 PBS. 02ｨ 050202 ｨｺ0502ｨｰｨｺｨｨ 070208ｨｬ02ｨ ｨ ｨｨ05ｨｨ04ｨｨ080609ｨ 05ｨｨ 09 0.5%-ｨｪ06ｨｬ 08ｨ 09ｨｰ09060802 0608ｨｨｨｰ06ｨｪｨ X-100 ｨｪｨ PBS 09 ｨｰ02 ﾂ02ｨｪｨｨ02 10 ｨｬｨｨｨｪ 09 070609050201ｨｮ06ｨｴ02ｨｦ ｨｰ080201ｨｺ08ｨ ｨｰｨｪ06ｨｦ 06ｨｰｨｬ0409ｨｺ06ｨｦ 09 PBS ｨｨ 10-ｨｬｨｨｨｪｨｮｨｰｨｪ06ｨｦ ｨｨｨｪｨｺｨｮｨ ｨ 02ｨｨ02ｨｦ 09 10%-ｨｪ06ｨｬ 08ｨ 09ｨｰ09060802 05ｨｬｨ 08ｨｨ06ｨｪｨ 05ｨｹｨｪ06ｨｦ 090409060806ｨｰｨｺｨｨ ｨｪｨ PBS 010507 0708020106ｨｰ0908ｨ ｨｴ02ｨｪｨｨ07 ｨｪ0209070202ｨｨ00ｨｨ ﾂ0209ｨｺ060006 090907040409ｨ ｨｪｨｨ07 ｨ ｨｪｨｰｨｨｨｰ0205. 05ｨ ｨｰ02ｨｬ 05ｨｬｨ 08ｨｨ06ｨｪ04 0706ｨｬ02ｨｴｨ 05ｨｨ 09 08ｨ 09ｨｰ090608 070208090401 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｨ ｨｨｨｪｨｺｨｮｨ ｨｨ080609ｨ 05ｨｨ 0708ｨｨ 4 縺05 ｨｪ02 ｨｬ02ｨｪ0202 12 ﾂ. 0306090502 06ｨｰｨｬ0409ｨｺｨｨ 09 PBS ｨｨ 10-ｨｬｨｨｨｪｨｮｨｰｨｪ06ｨｦ ｨｨｨｪｨｺｨｮｨ ｨ 02ｨｨｨｨ 09 05ｨｬｨ 08ｨｨ06ｨｪｨ 05ｨｹｨｪ06ｨｦ 090409060806ｨｰｨｺ02 04ｨ 0806010403ｨｨ 0706ｨｬ02ｨｴｨ 05ｨｨ 09 08ｨ 09ｨｰ090608 09ｨｰ06080401 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｪｨ 60 ｨｬｨｨｨｪ 0708ｨｨ ｨｺ06ｨｬｨｪｨ ｨｰｨｪ06ｨｦ ｨｰ02ｨｬ070208ｨ ｨｰｨｮ0802. 0306090502 06ｨｰｨｬ0409ｨｺｨｨ 09 PBS 05ｨｬｨ 08ｨｨ06ｨｪ04 04ｨ ｨｺ0506 ﾂｨ 05ｨｨ 09 09080201ｨｮ VectaShield (Vector Laboratories, 050108). 08ｨｪｨ 05ｨｨ04 07080207ｨ 08ｨ ｨｰ0609 070806090601ｨｨ05ｨｨ 09 0706ｨｬ06ｨｴｨｹ06 05ｨ 040208ｨｪ060006 09ｨｺｨ ｨｪｨｨ08ｨｮ06ｨｴ020006 ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ060006 ｨｬｨｨｨｺ080609ｨｺ0607ｨ Leica TSC SL. 00 ｨｺｨ ﾂ0209ｨｰ0902 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 ｨｨ09070605ｨｹ040609ｨ 05ｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ04, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 ｨｰ0605ｨｹｨｺ06 09ｨｰ060804ｨｬｨｨ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ. 02 ｨ 08 ｨ ｨ 06 ｨｰ ｨｺ ｨｮ 02 01 08 ｨ 04 06 ｨｦ (0.5 0003/ｨｬｨｺ05, Sigma, 050108) 070806090601ｨｨ05ｨｨ 09 ｨｰ02 ﾂ02ｨｪｨｨ02 1 ﾂ 0708ｨｨ 37 縺05. 06ｨｪｨｰ020008ｨ 05ｨｹｨｪｨｮ06 ｨｨ ｨｪ ｨｰ 02 ｨｪ 09 ｨｨ 09 ｨｪ 06 09 ｨｰ ｨｹ 00 05 ｨｮ 06 08 02 09 02 02 ｨｪ 02 ｨｨ ｨｨ ｨｪｨ ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ0401 ｨｨ0406ｨ 08ｨ 0302ｨｪｨｨ0701 060708020102050705ｨｨ 09 0706ｨｬ06ｨｴｨｹ06 0708060008ｨ ｨｬｨｬ04 ImageJ. 01ｨ ｨｺｨ 030106ｨｬ 0607ｨｰｨｨ ﾂ0209ｨｺ06ｨｬ 0908020402 070806ｨｨ04090601ｨｨ05ｨｨ 04ｨ ｨｬ020804 ｨｪｨ 5 0008ｨ 00ｨｬ02ｨｪｨｰｨ 01 070108ｨ ｨｨ 5 0008ｨ 00ｨｬ02ｨｪｨｰｨ 01 0006ｨｪｨ 09ｨｪ02 ｨｺ0502ｨｰｨｺｨｨ. 0604 ｨｺｨ 030106ｨｦ 090208ｨｨｨｨ ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ0401 ｨｨ0406ｨ 08ｨ 0302ｨｪｨｨｨｦ 07060106ｨ ｨｪ04ｨｬ 06ｨ 08ｨ 0406ｨｬ ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ 0706 5 0607ｨｰｨｨ ﾂ0209ｨｺｨｨ01 090802040609. 05ｨ ｨｰ02ｨｬ 01ｨ ｨｪｨｪ0402 ｨｮ09080201ｨｪ0705ｨｨ ｨｨ 08ｨ 0909 ﾂｨｨｨｰ0409ｨ 05ｨｨ 06ｨｰｨｪ060302ｨｪｨｨ02 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 070108ｨ ｨｺ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0006ｨｪｨ. 020507 ｨｺｨ 030106ｨｦ 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰｨ 05ｨｹｨｪ06ｨｦ

0508ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 01ｨ 08ｨ ｨｺｨｰ0208ｨ 0005ｨｮ060802090202ｨｪｨｰｨｪ060006 ｨｬ02 ﾂ02ｨｪｨｨ07 07010208 08ｨ ｨｪｨｪｨｨ01 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 833 04ｨｨ09. 2. 0906ｨｺｨ 05ｨｨ04ｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ. 06ｨｬｨ 08ｨｨ06ｨｪ04: ｨ ｪ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0402, ｨ ｪ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｪ0402 3 ﾂ in vitro, 09 ｪ 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ0402 020108ｨ 0406ｨｦ, 00 ｪ 0706090502 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 DRB, 01 ｪ 0706090502 ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨ07 09 ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪ06ｨｬ D. 05ｨｰ080205ｨｺｨ ｨｬｨｨ 0706ｨｺｨ 04ｨ ｨｪ06 ｨｬ02 ﾂ02ｨｪｨｨ02 06ｨｺ06050607010208ｨｪ06ｨｦ 0406ｨｪ04 02ｨｨｨｰ060705ｨ 04ｨｬ04, 090604ｨｪｨｨｨｺｨ 06ｨｴ0202 0708ｨｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 in vitro. 0008ｨｮ070704 ｨ ｨｪｨ 05ｨｨ04ｨｨ080609ｨ 05ｨｨ ｨｪ02 ｨｬ02ｨｪ0202 30 05ｨｬｨ 08ｨｨ06ｨｪ0609. 020507 070601ｨｰ090208030102ｨｪｨｨ07 010609ｨｰ06090208ｨｪ0609ｨｰｨｨ 08ｨ 04ｨｪｨｨ0204 ｨｬ020301ｨｮ 0904ｨ 0608ｨｺｨ ｨｬｨｨ ｨｨ09070605ｨｹ040609ｨ 05ｨｨ t-ｨｺ08ｨｨｨｰ0208ｨｨｨｦ 05ｨｰｨｹ060102ｨｪｨｰｨ. 040204ｨｮ05ｨｹｨｰｨ ｨｰ04 08ｨ ｨｺ 0607ｨｨ09ｨ ｨｪ06 08ｨ ｨｪ0202 (090600060506ｨ 0609ｨ, 090600060506ｨ 0609ｨ, 2009; 090600060506ｨ 0609ｨ, 03ｨ 080002ｨｪ0609, 2012), 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 07010208 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 09040302, ﾂ02ｨｬ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 02ｨｨｨｰ060705ｨ 04ｨｬ04, 0708ｨｨ 05ｨｰ06ｨｬ 06ｨ 08ｨ ｨｴｨ 02ｨｰ ｨｪｨ 0902ｨ 07 09ｨｪｨｨｨｬｨ ｨｪｨｨ02 ｨ 09090602ｨｨｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 070208ｨｨ000208ｨｨ02ｨｦ 070806070108040302ｨｺ (08ｨｨ09. 1, ｨ ). 0308ｨｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨｨｨ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609 09 ｨｰ02 ﾂ02ｨｪｨｨ02 3 ﾂ in vitro 01ｨ 08ｨ ｨｺｨｰ0208 ｨｬ02 ﾂ02ｨｪｨｨ07 ｨｪ02 ｨｨ04ｨｬ02ｨｪ0702ｨｰ0907 (ｨｪ02 0706ｨｺｨ 04ｨ ｨｪ06). 0308ｨｨ 06ｨ 08ｨ ｨ 06ｨｰｨｺ02 05ｨｬｨ 08ｨｨ06ｨｪ0609 020108ｨ 0406ｨｦ 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ02 07010208 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ 09060108ｨ ｨｪ0702ｨｰ0907, 0601ｨｪｨ ｨｺ06 01ｨ 08ｨ ｨｺｨｰ0208 020006 ｨｪ0209ｨｺ0605ｨｹｨｺ06 09ｨｨ0106ｨｨ04ｨｬ02ｨｪ0702ｨｰ0907 (08ｨｨ09. 1, ｨ ). 030802030102 0909020006, 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 09ｨｪｨｨ03ｨ 02ｨｰ0907 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｬ02 ﾂ02ｨｪｨｨ07 ｨｪｨ 070208ｨｨ000208ｨｨｨｨ 070806070108040302ｨｺ. 04ｨ 0907080201020502ｨｪｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 ｨｪｨｮｨｺ0502060705ｨ 04ｨｬ02 0708ｨｨ06ｨ 0802ｨｰｨ 02ｨｰ ｨｬ02ｨｪ0202 01ｨｨ0000ｨｮ04ｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208, 09 08070102 0905ｨｮ ﾂｨ 0209 ｨｬ0603ｨｪ06 ｨｪｨ ｨ 050601ｨ ｨｰｨｹ 070607090502ｨｪｨｨ02 06ｨｰ010205ｨｹｨｪ0401 06ｨ 05ｨ 09ｨｰ02ｨｦ ｨ 06050202 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ060006 ｨｬ02 ﾂ02ｨｪｨｨ07. 05050201ｨｮ02ｨｰ ｨｰｨ ｨｺ0302 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ, ﾂｨｰ06 09 ｨ 0605ｨｹ03ｨｨｨｪ09ｨｰ0902 0905ｨｮ ﾂｨ 0209 070108ｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609, 06ｨ 08ｨ ｨ 06ｨｰｨ ｨｪｨｪ0401 020108ｨ 0406ｨｦ, ｨｰ02080706ｨｰ 06ｨｺ08ｨｮ0005ｨｮ06 000608ｨｬｨｮ (08ｨｨ09. 1, ｨ ). 0308ｨｨ ｨｨｨｪ00ｨｨｨ ｨｨ080609ｨ ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｺｨ ｨｺ 09 0706ｨｬ06ｨｴｨｹ06 DRB (08ｨｨ09. 1, 09), ｨｰｨ ｨｺ ｨｨ 09 0706ｨｬ06ｨｴｨｹ06 ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪｨ D (08ｨｨ09. 1, 00) 06ｨ 08ｨ ｨｴｨ 02ｨｰ ｨｪｨ 0902ｨ 07 09ｨｪｨｨｨｬｨ ｨｪｨｨ02 ｨｮ09ｨｨ0502ｨｪｨｨ02 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ ｨｪｨ 070208ｨｨ000208ｨｨｨｨ 070806070108040302ｨｺ. 0308ｨｨ 05ｨｰ06ｨｬ ｨｬ02ｨｪ0702ｨｰ0907 ｨｪ02 ｨｰ0605ｨｹｨｺ06 0202 09ｨｨ04ｨｮｨ 05ｨｹｨｪｨ 07 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ, ｨｪ06 ｨｨ 09ｨ ｨｬ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｬ02 ﾂ02ｨｪｨｨ07. 00ｨ 05ｨｨ04ｨｨ 070806070108040302ｨｺ ｨｪｨ ﾂｨｨｨｪｨ 06ｨｰ 090407090507ｨｰｨｹ0907 ｨｬｨｪ060006 ﾂｨｨ090502ｨｪｨｪ0402 070506ｨｰｨｪ0402 06ｨｺ08ｨｮ00050402 06ｨ 05ｨ 09ｨｰｨｨ ｨ 06050202 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ06ｨｦ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ (08ｨｨ09. 1, 09, 00). 0306090502 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ 020108ｨ 0406ｨｦ ｨｺ0605ｨｨ ﾂ0209ｨｰ0906 07060106ｨ ｨｪ0401 09ｨｰ08ｨｮｨｺｨｰｨｮ08 ｨｨ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｨ01 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ ｨｮｨｬ02ｨｪｨｹ03ｨ 06ｨｰ0907 (08ｨｨ09. 1, 01). 05010601ｨｪ0402 0706 000608ｨｬ02 ｨｨ 08ｨ 04ｨｬ0208ｨ ｨｬ 09ｨｰ08ｨｮｨｺｨｰｨｮ0804 09 ｨ 0605ｨｹ0306ｨｬ ｨｺ0605ｨｨ ﾂ0209ｨｰ0902 0909ｨｰ0802 ﾂｨ 06ｨｰ0907 ｨｨ 09 0202ｨｪｨｰ08ｨ 05ｨｹｨｪ0401 06ｨ 05ｨ 09ｨｰ0701 ｨｪｨｮｨｺ0502060705ｨ 04ｨｬ04 (08ｨｨ09. 1, 01), ｨｺ06ｨｰ0608ｨ 07 070605ｨｪ0609ｨｰｨｹ06 ｨｰ02080702ｨｰ 01ｨｨ0000ｨｮ04ｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208 ｨｬ02 ﾂ02ｨｪｨｨ07, ｨｪｨ ｨ 050601ｨ 02ｨｬ04ｨｦ 09 ｨｺ06ｨｪｨｰ08060502 (08ｨｨ09. 1, ｨ ). 0306090502 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ, 090408ｨ ｨ 06ｨｰｨ ｨｪｨｪ04ｨｬｨｨ ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ (08ｨｨ09. 2, ｨ ), ｨｪｨ 070806ｨｰｨｨ09, ｨｺｨ ｨｺ ｨｮ0302 090606ｨ ｨｴｨ 050609ｨｹ, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 02ｨｨｨｰ060705ｨ 04ｨｬ04 09040302, ﾂ02ｨｬ 070108ｨ (090600060506ｨ 0609ｨ, 090600060506ｨ 0609ｨ, 2009). 0002 ﾂ02ｨｪｨｨ02 ｨｪｨｮｨｺ0502060705ｨ 04ｨｬ04 ｨｨｨｬ0202ｨｰ ｨｬ0205ｨｺ0601ｨｨ0907020809ｨｪ04ｨｦ 01ｨ 08ｨ ｨｺｨｰ0208, ｨ 09090602ｨｨｨ 02ｨｨ07 09040709050702ｨｬ060006 ｨ ｨｺｨｰｨｨｨｪｨ 09 070208ｨｨ000208ｨｨ02ｨｦ 070806070108040302ｨｺ ｨｪ02 090408ｨ 0302ｨｪｨ. 0308ｨｨ ｨｺｨｮ05ｨｹｨｰｨｨ09ｨｨ080609ｨ ｨｪｨｨｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 09 ｨｰ02 ﾂ02ｨｪｨｨ02 3 ﾂ in vitro 07ｨ ｨｰｨｰ0208ｨｪ ｨｬ02 ﾂ02ｨｪｨｨ07 ｨｪｨｮｨｺ0502060705ｨ 04ｨｬ04 ｨｪ02 ｨｬ02ｨｪ0702ｨｰ0907, 0601ｨｪｨ ｨｺ06 09 06ｨ 05ｨ 09ｨｰｨｨ 02ｨｨｨｰ060705ｨ 04ｨｬ04, 07060008ｨ ｨｪｨｨ ﾂｨｪ06ｨｦ 09 07010806ｨｬ, ｨｬ02 ﾂ02ｨｪｨｨ02 09ｨｰｨ ｨｪ0609ｨｨｨｰ0907 ｨ 06050202 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ04ｨｬ (08ｨｨ09. 2, ｨ ), ﾂ020006 ｨｪｨｨｨｺ060001ｨ ｨｪ02 ｨｪｨ ｨ 050601ｨ 02ｨｰ0907 ｨｮ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609, 08ｨ 0409ｨｨ09ｨ 06ｨｴｨｨ010907 in vivo. 0308ｨｨ 06ｨ 08ｨ ｨ 06ｨｰｨｺ02 05ｨｬｨ 08ｨｨ06ｨｪ0609 020108ｨ 0406ｨｦ ｨｰｨ ｨｺ0302 06ｨｰｨｬ02 ﾂｨ 02ｨｰ0907 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ02 000608ｨｬ04 07010208, 0601ｨｪｨ ｨｺ06 ｨｺｨ ｨｺ060006-05ｨｨｨ 06 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 07ｨ ｨｰｨｰ0208ｨｪｨ 06ｨｺ08ｨ 03ｨｨ09ｨ ｨｪｨｨ07 ｨｪｨ ｨｬｨｨ ｨｪ02 090407090502ｨｪ06 (08ｨｨ09. 2, 09). 0308ｨｨ ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ06ｨｬ 070601ｨ 090502ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ06ｨｪｨｪ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ ｨｪ0204ｨ -

13834 06. 02. 090600060506ｨ 0609ｨ 04ｨｨ09. 3. 0604ｨｬ02ｨｪ02ｨｪｨｨ02 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 07010208 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ ｨｪｨ ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ0401 ｨｨ0406ｨ 08ｨ 0302ｨｪｨｨ0701 0706090502 0906040102ｨｦ09ｨｰ09ｨｨ07 DRB ｨｨ 020108ｨ 0404. ｨ ｪ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ, ｨ ｪ ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ. 000208ｨｰｨｨｨｺｨ 05ｨｹｨｪ0402 06ｨｰ080204ｨｺｨｨ 090606ｨｰ0902ｨｰ09ｨｰ09ｨｮ06ｨｰ 0008ｨ ｨｪｨｨ02ｨ ｨｬ 0106090208ｨｨｨｰ0205ｨｹｨｪ0401 ｨｨｨｪｨｰ020809ｨ 050609 010507 ｨｮ080609ｨｪ07 04ｨｪｨ ﾂｨｨｨｬ0609ｨｰｨｨ 0.05. 050902040106 ﾂｨｺｨ ｨｬｨｨ 06ｨ 0604ｨｪｨ ﾂ02ｨｪ04 04ｨｪｨ ﾂ02ｨｪｨｨ07, 010609ｨｰ06090208ｨｪ06 06ｨｰ05ｨｨ ﾂｨ 06ｨｴｨｨ020907 06ｨｰ ｨｺ06ｨｪｨｰ08060507. 09ｨｨ09ｨｨｨｬ06 06ｨｰ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｪ060006 ｨｨｨｪ00ｨｨｨ ｨｨｨｰ0608ｨ ｨｰｨ ｨｺ0302 ｨｪ02 ｨｪｨ ｨ 050601ｨ 02ｨｰ0907 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 01ｨ 08ｨ ｨｺｨｰ0208ｨ 0005ｨｮ060802090202ｨｪｨｰｨｪ060006 ｨｬ02 ﾂ02ｨｪｨｨ07 ｨｪｨｮｨｺ0502060705ｨ 04ｨｬ04 (08ｨｨ09. 2, 00, 01). 02ｨｰｨｬ02 ﾂｨ 02ｨｰ0907 ｨｰ0605ｨｹｨｺ06 ｨｮ0302 0607ｨｨ09ｨ ｨｪｨｪｨ 07 09040302 0005ｨｮ060802090202ｨｪ02ｨｨ07 06ｨｺ06050607010208ｨｪ06ｨｦ 06ｨ 05ｨ 09ｨｰｨｨ 02ｨｨｨｰ060705ｨ 04ｨｬ04, ﾂｨｰ06, ｨｺｨ ｨｺ 0706ｨｺｨ 040409ｨ 06ｨｰ ｨｺ06ｨｪｨｰ080605ｨｹｨｪ0402 05ｨｺ09070208ｨｨｨｬ02ｨｪｨｰ04, 09090704ｨ ｨｪ06 09 ｨｮ09050609ｨｨ07ｨｬｨｨ in vitro, ｨ ｨｪ02 09 0102ｨｦ09ｨｰ09ｨｨ02ｨｬ ｨｨｨｪ00ｨｨｨ ｨｨｨｰ06080609 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ. 00 0106070605ｨｪ02ｨｪｨｨ02 ｨｺ 09ｨｨ04ｨｮｨ 05ｨｹｨｪ04ｨｬ ｨｪｨ ｨ 05060102ｨｪｨｨ07ｨｬ ｨｪｨ ｨｬｨｨ ｨ 0405 07080609020102ｨｪ 0908ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 ｨｨｨｪｨｰ020008ｨ 05ｨｹｨｪ06ｨｦ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ ｨｪｨ ｨｺ06ｨｪ0006ｨｺｨ 05ｨｹｨｪ0401 ｨｨ0406ｨ 08ｨ 0302ｨｪｨｨ0701 (08ｨｨ09. 3). 0306090502 ｨｨｨｪ00ｨｨｨ ｨｨ080609ｨ ｨｪｨｨ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 09 0706ｨｬ06ｨｴｨｹ06 DRB 09 0905ｨｮ ﾂｨ 02 ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨ07 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨｨｦ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 07010208 05ｨｬｨ 08ｨｨ06ｨｪ0609 0706 0908ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ06ｨｪｨｰ08060502ｨｬ ｨｪ02 090407090502ｨｪ06 (4.69 ﾀ 1 7 0.57 ｨｨ 4.89 ﾀ 0.30 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06). 0308ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ C-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ 090407090502ｨｪ06 09ｨｮｨｴ0209ｨｰ0902ｨｪｨｪ0602 ｨｮ090205ｨｨ ﾂ02ｨｪｨｨ02 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｨ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 0706 0908ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ06ｨｪｨｰ08060502ｨｬ (6.23 ﾀ 0.66 ｨｨ 4.10 ﾀ 0.46 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06). 06ｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 07010208, ｨｬ02 ﾂ02ｨｪｨｪ0401 09 0706ｨｬ06ｨｴｨｹ06 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ, ｨｪ02 ｨｬ02ｨｪ0705ｨ 09ｨｹ 0706090502 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 020108ｨ 0406ｨｦ (4.42 ﾀ 0.76 070806ｨｰｨｨ09 4.89 ﾀ 1 7 0.30 09 ｨｺ06ｨｪｨｰ08060502). 01ｨ 070806ｨｰｨｨ09, ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 0005ｨｮ060802090202ｨｪ02ｨｨｨｨ 07010208, 06ｨｺ08ｨ 0302ｨｪｨｪ0401 09 0706ｨｬ06ｨｴｨｹ06 ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ, ｨｮ090205ｨｨ ﾂｨｨ09ｨ 05ｨ 09ｨｹ 0706090502 06ｨ 08ｨ ｨ 06ｨｰｨｺｨｨ 020108ｨ 0406ｨｦ 0706 0908ｨ 09ｨｪ02ｨｪｨｨ06 09 ｨｺ06ｨｪｨｰ08060502ｨｬ 0706 ﾂｨｰｨｨ 09 2 08ｨ 04ｨ (8.62 ﾀ 0.98 ｨｨ 4.10 ﾀ 0.46 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06). 02ｨ 09ｨｮ030102ｨｪｨｨ02 05050201ｨｮ02ｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ, ﾂｨｰ06 070601ｨ 09050706ｨｴｨ 07 ﾂｨ 09ｨｰｨｹ ｨｨ09090502010609ｨ ｨｪｨｨｨｦ, 09 0202ｨｪｨｰ0802 09ｨｪｨｨｨｬｨ ｨｪｨｨ07 ｨｺ06ｨｰ06080401 ｨｪｨ 010601ｨｨｨｰ0907 07010208ｨｪ04ｨｦ ｨ ｨｺｨｰｨｨｨｪ, 070806090601ｨｨｨｰ0907 ｨｪｨ ｨｺｨｮ05ｨｹｨｰｨｮ08ｨ 01 0906ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 ｨｺ0502ｨｰ06ｨｺ 09 0708ｨｨｨｬ02ｨｪ02ｨｪｨｨ02ｨｬ 09 07020809ｨｮ06 06 ﾂ02080201ｨｹ ｨ ｨｨ0601ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ01 ｨｨ ｨｬ060502ｨｺｨｮ050708ｨｪ06-ｨ ｨｨ06050600ｨｨ ﾂ0209ｨｺｨｨ01 ｨｬ02ｨｰ0601ｨｨｨｺ. 030605ｨｰ06ｨｬｨｮ, ｨｪ0209ｨｬ06ｨｰ0807 ｨｪｨ ｨ ｨｺｨｰｨｨ09ｨｪ0402 ｨｨ09090502010609ｨ ｨｪｨｨ07 080605ｨｨ ｨ ｨｺｨｰｨｨｨｪｨ 09 07080602020909ｨ 01 07010208ｨｪ060006 ｨｬ02ｨｰｨ ｨ 0605ｨｨ04ｨｬｨ, 01ｨ ｨｪｨｪ0402 06 08ｨ 0907080201020502ｨｪｨｨｨｨ 05ｨｰ060006 ｨ 0205ｨｺｨ 09 09ｨｨ09ｨｰ02ｨｬ02 ｨｰ080201ｨｬ0208ｨｪ06ｨｦ 07010208ｨｪ06ｨｦ ｨ 0801ｨｨｨｰ02ｨｺｨｰ06ｨｪｨｨｨｺｨｨ 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｨ05ｨｨ 060602ｨｨｨｰ0609 ｨｬ0502ｨｺ0607ｨｨｨｰｨ 06ｨｴｨｨ01 0609ｨｰｨ 06ｨｰ0907 ｨｺ08ｨ ｨｦｨｪ02 09ｨｺｨｮ01ｨｪ04ｨｬｨｨ ｨｨ 08ｨ 04080604ｨｪ02ｨｪｨｪ04ｨｬｨｨ (Nguyen et al., 1998; Johnson et al., 2003; Bohnsack et al., 2006; 090600060506ｨ 0609ｨ, 090600060506ｨ 0609ｨ, 2009; 090600060506ｨ 0609ｨ, 03ｨ 080002ｨｪ0609, 2012). 020507 0906ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 ｨｺ0502ｨｰ06ｨｺ ｨｬ0502ｨｺ0607ｨｨｨｰｨ 06ｨｴｨｨ01 ｨｮｨ 0201ｨｨｨｰ0205ｨｹｨｪ06 0708060102ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ ｨｪ06 ｨｮ ﾂｨ 09ｨｰｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 07080602020909ｨ 01 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ, 09 ｨｰ06ｨｬ ﾂｨｨ090502 09 ｨｮ ﾂｨ 09ｨｰｨｨ02ｨｬ 040108-070605ｨｨｨｬ0208ｨ 0404 II (Miralles, Visa, 2006; Obrdlik et al., 2007; Pederson, 2008; Hofmann 2009; Visa, Percipalle, 2010). ﾁｨｰ06 ｨｺｨ 09ｨ 02ｨｰ0907 0909020102ｨｪｨｨｨｦ 06 0702080208ｨ 0907080201020502ｨｪｨｨｨｨ ｨ ｨｺｨｰｨｨｨｪｨ 09 ｨｮ09050609ｨｨ0701 09ｨｪｨｨ0302ｨｪｨｨ07 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ06ｨｪｨｪ06ｨｦ ｨ ｨｺｨｰｨｨ09ｨｪ0609ｨｰｨｨ, ｨｰ06 06ｨｪｨｨ ｨｺ08ｨ ｨｦｨｪ02 ｨｪ02ｨｬｨｪ060006- ﾂｨｨ090502ｨｪｨｪ04. 00 ﾂｨ 09ｨｰｨｪ0609ｨｰｨｨ, 0706ｨｺｨ 04ｨ ｨｪ06 070607090502ｨｪｨｨ02 09 07010802 0902ｨｰｨｨ ｨ ｨｺｨｰｨｨｨｪ06090401 00ｨｨ05ｨ ｨｬ02ｨｪｨｰ0609 0708ｨｨ ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ06ｨｬ 070601ｨ 090502ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 09 060602ｨｨｨｰｨ 01 ｨ ｨｬ00ｨｨｨ ｨｨｨｦ (Scheer et al., 1984). 06ｨｬ0206ｨｰ0907 0909020102ｨｪｨｨ07 06 ｨｪｨ ｨｺ06070502ｨｪｨｨｨｨ ｨ ｨｺｨｰｨｨｨｪｨ 09 ｨｺ05ｨ 09ｨｰ0208ｨ 01 ｨｨｨｪｨｰ0208010806ｨｬｨ ｨｰｨｨｨｪ06090401 0008ｨ ｨｪｨｮ05 09 ｨ ｨｪｨ 050600ｨｨ ﾂｨｪ0401 ｨｮ09050609ｨｨ0701 (Wang et al., 2006). 00 ｨｪｨ 0302ｨｦ 08ｨ ｨ 06ｨｰ02 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ02 09ｨｪｨｮｨｰ08ｨｨ07010208ｨｪ060006 07ｨ ｨｰｨｰ0208ｨｪｨ 08ｨ 0907080201020502ｨｪｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 0708ｨｨ ｨｨ09ｨｺｨｮ0909ｨｰ0902ｨｪｨｪ06ｨｬ 070601ｨ 090502ｨｪｨｨｨｨ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ ｨｬ0603ｨｪ06 ｨ 040506 ｨｪｨ ｨ 050601ｨ ｨｰｨｹ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ, ｨｪ06 ｨｪ02 ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ. 0308ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ DRB ｨｨ ｨ ｨｺｨｰｨｨｨｪ06ｨｬｨｨ02ｨｨｨｪｨ D ｪ ｨｨｨｪ00ｨｨｨ ｨｨｨｰ06080609 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 09 08ｨ 04ｨｪ04ｨｬｨｨ ｨｬ0201ｨ ｨｪｨｨ04ｨｬｨ ｨｬｨｨ 0102ｨｦ09ｨｰ09ｨｨ07 (Perry, Kelley, 1970; Sehgal et al., 1976; Zandomeni, Weinmann, 1984) ｪ 08ｨ 0907080201020502ｨｪｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｨ04ｨｬ02ｨｪ07050609ｨｹ 09010601ｨｪ04ｨｬ 06ｨ 08ｨ 0406ｨｬ. 00 06ｨ 06ｨｨ01 0905ｨｮ ﾂｨ 0701 06ｨｰｨｬ02 ﾂｨ 05ｨ 09ｨｹ ｨｺ06ｨｪ0202ｨｪｨｰ08ｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 0406ｨｪｨ 01, ｨ 09090602ｨｨｨｨ080609ｨ ｨｪｨｪ0401 09 070208ｨｨ000208ｨｨ02ｨｦ 070806070108040302ｨｺ. 01ｨ 0906ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 ｨｺ0502ｨｰｨｺｨ 01 ｨｬ0502ｨｺ0607ｨｨｨｰｨ 06ｨｴｨｨ01 ｨ 040506 0706ｨｺｨ 04ｨ ｨｪ06, ﾂｨｰ06 0708ｨｨ 0209ｨｰ0209ｨｰ0902ｨｪｨｪ06ｨｬ 04ｨ ｨｬ02010502ｨｪｨｨｨｨ 070806020209090609 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ (09 090609ｨｰ0607ｨｪｨｨｨｨ 04ｨｨｨｬｨｪ02ｨｦ 090707 ﾂｨｺｨｨ) ｨｨｨｬ0202ｨｰ ｨｬ0209ｨｰ06 ｨｰ0209ｨｪ0602 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨ02 ｨｬ020301ｨｮ ｨｰ0205ｨｹ02ｨ ｨｬｨｨ 08ｨ 01ｨ 05ｨ ｨｨ 0701080403ｨｺｨ ｨｬｨｨ (Malatesta et al., 1994). 0308060701080403ｨｺｨｨ ｪ 0909060206ｨ 08ｨ 04ｨｪ0402 09ｨｰ08ｨｮｨｺｨｰｨｮ0804, 01ｨ 08ｨ ｨｺｨｰ0208ｨｪ0402 010507 08ｨ ｨｪｨｪｨｨ01 09ｨｰｨ 01ｨｨｨｦ 08ｨ 0409ｨｨｨｰｨｨ07 ｨｬ0502ｨｺ0607ｨｨｨｰｨ 06ｨｴｨｨ01, 0907060905020109ｨｰ09ｨｨｨｨ 01ｨ 06ｨｴｨｨ02 ｨｪｨ ﾂｨ 0506 ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨ06ｨｪｨｪ06 ｨ ｨｺｨｰｨｨ09ｨｪ04ｨｬ 0701080403ｨｺｨ ｨｬ (Fl*echon, Kopecny, 1998). 080806ｨｬ02 ｨｰ060006, 0708060102ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ ｨｪ06 ｨｨ01 ｨｮ ﾂｨ 09ｨｰｨｨ02 09 07080602020909ｨ 01 ｨ ｨｨ060002ｨｪ0204ｨ ｨｰ02050202 08ｨ 01ｨ 05ｨ (Ferreira, Carmo-Fonseca, 1995). 0102 ｨｨ09ｨｺ0506 ﾂ02ｨｪ06, ﾂｨｰ06 090702ｨｺｨｰ08 00ｨｮｨｪｨｺ02ｨｨｨｦ 05ｨｰｨｨ01 07010208ｨｪ0401 0106ｨｬ02ｨｪ0609 04ｨｪｨ ﾂｨｨｨｰ0205ｨｹｨｪ06 03ｨｨ0802, ﾂ02ｨｬ 07080201070605ｨ 00ｨ 02ｨｰ0907 0902000601ｨｪ07. 060209ｨｪ0602 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ0602 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨ02 ｨ ｨｺｨｰｨｨｨｪｨ 09 010806ｨｬｨ ｨｰｨｨｨｪ06ｨｬ 0708060102ｨｬ06ｨｪ09ｨｰ08ｨｨ080609ｨ ｨｪ06 09 02020506ｨｬ 08070102 08ｨ ｨ 06ｨｰ, 070802030102 0909020006 ｨｪｨ 0906ｨｬｨ ｨｰｨｨ ﾂ0209ｨｺｨｨ01 ｨｺ0502ｨｰｨｺｨ 01 ｨｬ0502ｨｺ0607ｨｨｨｰｨ 06ｨｴｨｨ01 (Papoulas et al., 1998; Zhao et al., 1998; Shen et al., 2000; Hofmann, 2009; Visa, Percipalle, 2010). 000603ｨｪ06 ｨ 040506 0603ｨｨ01ｨ ｨｰｨｹ, ﾂｨｰ06 0708ｨｨ 06ｨ 08ｨ ｨ 06ｨｰｨｺ02 07080207ｨ 08ｨ ｨｰ0609 020108ｨ 0406ｨｦ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｬ02 ﾂ02ｨｪｨｨ07 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ ｨ ｨｮ0102ｨｰ 09ｨｪｨｨ03ｨ ｨｰｨｹ0907 04ｨ 09 ﾂ02ｨｰ ｨｮ01ｨ 0502ｨｪｨｨ07 07ｨｮ05ｨ ｨｬ060502ｨｺｨｮ05, ｨｪｨ 01060107ｨｴｨｨ010907 09 070807ｨｬ06ｨｦ ｨ 09090602ｨｨｨ 02ｨｨｨｨ 09 020108. 0201ｨｪｨ ｨｺ06 ｨｬ04 ｨｪｨ ｨ 050601ｨ 05ｨｨ 070807ｨｬ06 070806ｨｰｨｨ09060706050603ｨｪ04ｨｦ 080204ｨｮ05ｨｹｨｰｨ ｨｰ: ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｬ02 ﾂ02ｨｪｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504 ｨｮ090205ｨｨ ﾂｨｨ09ｨ 05ｨ 09ｨｹ. 02ｨ ｨｪｨｪ0602 ｨｪｨ ｨ 05060102ｨｪｨｨ02 ｨｬ0603ｨｪ06 06ｨ ｨｲ0709ｨｪｨｨｨｰｨｹ ｨｰ02ｨｬ, ﾂｨｰ06 09 ｨｪｨ ｨｰｨｨ09ｨｪ06ｨｬ 090609ｨｰ0607ｨｪｨｨｨｨ ﾂｨ 09ｨｰｨｹ ｨｬ060502ｨｺｨｮ05 ｨ ｨｺｨｰｨｨｨｪｨ, ｨｪｨ 01060107ｨｴｨｨ010907 09 070807ｨｬ06ｨｬ 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨｨｨ ｨｪ02 09 020108, ｨ 09 ｨ 0205ｨｺｨ ｨｬｨｨ 010806ｨｬｨ ｨｰｨｨｨｪｨ, ｨｬ060302ｨｰ ｨ 04ｨｰｨｹ 04ｨ ｨｺ0804ｨｰｨ 010507 0904ｨ ｨｨｨｬ060102ｨｦ09ｨｰ09ｨｨ07 09 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｬｨｨ. 0308ｨｨ ｨｮ01ｨ 0502ｨｪｨｨｨｨ 020108 09ｨ ｨｦｨｰ04 090907040409ｨ ｨｪｨｨ07 09ｨｰｨ ｨｪ060907ｨｰ0907 ｨ 06050202 010609ｨｰｨｮ07ｨｪ04ｨｬｨｨ 010507 0102ｨｦ09ｨｰ09ｨｨ07 ｨ ｨｪｨｰｨｨｨｰ0205, ｨｨ ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ ｨｬ02 ﾂ02ｨｪｨｨ07 09060408ｨ 09ｨｰｨ 02ｨｰ. 00 04ｨ ｨｺ0506 ﾂ02ｨｪｨｨ02 09050201ｨｮ02ｨｰ 06ｨｰｨｬ02ｨｰｨｨｨｰｨｹ, ﾂｨｰ06 090902 ｨｪｨ ｨ 050601ｨ 02ｨｬ0402 ｨｨ04ｨｬ02ｨｪ02ｨｪｨｨ07 08ｨ 0907080201020502ｨｪｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 05ｨｬｨ 08ｨｨ06ｨｪ0609 06ｨｰｨｬ02 ﾂｨ 05ｨｨ09ｨｹ ｨｰ0605ｨｹｨｺ06 0708ｨｨ ｨｨ09070605ｨｹ040609ｨ ｨｪｨｨｨｨ ｨ ｨｪｨｰｨｨｨｰ0205 ｨｺ 05-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ ｨｬ060502ｨｺｨｮ0504. 04ｨ ｨｪ0202 ｨｬ04 07080201070605ｨ 00ｨ 05ｨｨ, ﾂｨｰ06 ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ 05- ｨｨ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨ ｨｬ ｨｬ060502ｨｺｨｮ0504 ｨ ｨｺｨｰｨｨｨｪｨ 09040709050706ｨｰ 08ｨ 04ｨｪ0402 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ0402 000608ｨｬ04 ｨ ｨｺｨｰｨｨｨｪｨ, ｨｪｨ 01060107ｨｴｨｨ020907 09 00ｨｮｨｪｨｺ02ｨｨ06ｨｪｨ 05ｨｹｨｪ0401 0904ｨ ｨｨｨｬ06090907040701 09 010806ｨｬｨ ｨｰｨｨｨｪ06ｨｬ ｨｨ 09 ｨ 0707ｨ 08ｨ ｨｰ06ｨｬ ｨｰ08ｨ ｨｪ09ｨｺ08ｨｨ0702ｨｨｨｨ 090606ｨｰ0902ｨｰ09ｨｰ0902ｨｪｨｪ06 (090600060506ｨ 0609ｨ, 090600060506ｨ 0609ｨ, 2009). 02ｨ ｨｪｨｪ0402, 070605ｨｮ ﾂ02ｨｪｨｪ0402 09 ｨｪｨ 09ｨｰ0607ｨｴ02ｨｦ

130508ｨ 09ｨｪｨｨｨｰ0205ｨｹｨｪ04ｨｦ ｨ ｨｪｨ 05ｨｨ04 01ｨ 08ｨ ｨｺｨｰ0208ｨ 0005ｨｮ060802090202ｨｪｨｰｨｪ060006 ｨｬ02 ﾂ02ｨｪｨｨ07 07010208 08ｨ ｨｪｨｪｨｨ01 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ 835 08ｨ ｨ 06ｨｰ02, 0909ｨｨ0102ｨｰ0205ｨｹ09ｨｰ09ｨｮ06ｨｰ 070806ｨｰｨｨ09 01ｨ ｨｪｨｪ060006 07080201070605060302ｨｪｨｨ07. 0906050202 0902080607ｨｰｨｪ06, ﾂｨｰ06, ｨｪ0209ｨｬ06ｨｰ0807 ｨｪｨ 09010601ｨｪｨｮ06 ｨｨｨｪｨｰ02ｨｪ09ｨｨ09ｨｪ0609ｨｰｨｹ 090907040409ｨ ｨｪｨｨ07 01ｨ ｨｪｨｪ0401 ｨ ｨｪｨｰｨｨｨｰ0205 09 ｨ ｨｺｨｰｨｨｨｪ06ｨｬ ｨｪｨ ｨｨｨｬｨｬｨｮｨｪ06ｨ 0506ｨｰｨ 01 (090600060506ｨ 0609ｨ, 03ｨ 080002ｨｪ0609, 2012), ｨ ｨｪｨｰｨｨｨｰ0205ｨ ｨｺ N-ｨｺ06ｨｪ02020906ｨｬｨｮ 0106ｨｬ02ｨｪｨｮ 09 09ｨｨ05ｨｮ 090906ｨｨ01 00ｨｨ04ｨｨｨｺ06-01ｨｨｨｬｨｨ ﾂ0209ｨｺｨｨ01 060906ｨ 02ｨｪｨｪ0609ｨｰ02ｨｦ 06ｨ 05ｨ 01ｨ 06ｨｰ ｨｬ02ｨｪｨｹ0302ｨｦ 0907060906ｨ ｨｪ0609ｨｰｨｹ06 090907040409ｨ ｨｰｨｹ0907 09 07010208ｨｪ04ｨｬ ｨ ｨｺｨｰｨｨｨｪ06ｨｬ 0708ｨｨ ｨｬ02 ﾂ02ｨｪｨｨｨｨ ｨｰ06ｨｰｨ 05ｨｹｨｪ0401 07080207ｨ 08ｨ ｨｰ0609 05ｨｬｨ 08ｨｨ06ｨｪ0609 ｨｬ0403ｨｨ. 04ｨ ｨ 06ｨｰｨ 0904070605ｨｪ02ｨｪｨ 0708ｨｨ 00ｨｨｨｪｨ ｨｪ09060906ｨｦ 07060101020803ｨｺ02 04060909ｨｨｨｦ09ｨｺ060006 0006ｨｪ01ｨ 00ｨｮｨｪ01ｨ ｨｬ02ｨｪｨｰｨ 05ｨｹｨｪ0401 ｨｨ09090502010609ｨ ｨｪｨｨｨｦ (07080602ｨｺｨｰ 10-04-00757) ｨｨ 0708060008ｨ ｨｬｨｬ04 040801 0000060502ｨｺｨｮ050708ｨｪｨ 07 ｨｨ ｨｺ0502ｨｰ06 ﾂｨｪｨ 07 ｨ ｨｨ06050600ｨｨ0703. 0507ｨｨ0906ｨｺ 05ｨｨｨｰ0208ｨ ｨｰｨｮ0804 090600060506ｨ 0609ｨ 01. 08., 090600060506ｨ 0609ｨ 06. 02. 2009. 0906ｨｺｨ 05ｨｨ04ｨ 02ｨｨ07 ｨ ｨｺｨｰｨｨｨｪｨ 09 070108ｨ 01 0109ｨｮ01ｨｺ0502ｨｰ06 ﾂｨｪ0401 04ｨ 080601040302ｨｦ ｨｬ0403ｨｨ. 00ｨｨｨｰ06050600ｨｨ07. 51 (8) : 663 ｪ669. 090600060506ｨ 0609ｨ 06. 02., 03ｨ 080002ｨｪ0609 00. 01. 2012. 020906ｨ 02ｨｪｨｪ0609ｨｰｨｨ ｨｨｨｬｨｬｨｮｨｪ060005ｨｮ060802090202ｨｪｨｰｨｪ060006 090407090502ｨｪｨｨ07 07010208ｨｪ060006 ｨ ｨｺｨｰｨｨｨｪｨ 09 08ｨ ｨｪｨｪｨｨ01 05ｨｬｨ 08ｨｨ06ｨｪｨ 01 ｨｬ0403ｨｨ. 00ｨｨｨｰ06050600ｨｨ07. 54 (7) : 541 ｪ548. 03ｨ 080002ｨｪ0609 00. 01., 01ｨ 05ｨ ｨｺｨｰｨｨ06ｨｪ0609 08. 06. 1987. 00ｨｪｨｮｨｰ08ｨｨ07010208ｨｪ0402 ｨ ｨｺｨｰｨｨｨｪ06090402 ｨｬｨｨｨｺ080600ｨｨ05ｨ ｨｬ02ｨｪｨｰ04 09 060602ｨｨｨｰｨ 01 ｨｰ08ｨ 0907ｨｪ06ｨｦ 050700ｨｮ03ｨｺｨｨ. 00ｨｨｨｰ06050600ｨｨ07. 29(2) : 142 ｪ149. 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Inhibitory effect of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole on a protein kinase. J. Biol. Chem. 259 : 14 804 ｪ14 811. Zhao K., Wang W., Rando O. J., Xue Y., Swiderek K., Kuo A., Cradtree G. R. 1998. Rapid and phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T-lymphocyte receptor signalling. Cell. 95 : 625 ｪ636. 030609ｨｰｨｮ07ｨｨ05ｨ 2 VII 2012 A COMPARATIVE STUDY OF FLUORESCENT PATTERNS IN THE NUCLEI OF EARLY MOUSE EMBRYOS USING ANTIBODIES AGAINST DIFFERENT DOMAINS OF THE ACTIN MOLECULE I. O. Bogolyubova Institute of Cytology RAS, St. Petersburg; mail: ibogol@mail.ru In this work, new data on the peculiarities of actin immunofluorescent detection in 2-cell mouse embryos using antibodies against C- and N-terminal domains are presented. We studied the distribution of nuclear actin after artificial suppression of transcription and after enzymatic digestion of DNA. The visual observations were

13836 06. 02. 090600060506ｨ 0609ｨ supplemented with morphometric analyses of confocal images. In both experimental groups (treated by transcription inhibitors or DNase) the reliable increasing of fluorescence intensity is revealed when antibody against C-, but not against N-terminal domain of actin was used. These finding allows to suppose that antibody against C-terminal domain of actin is suitable with a high efficiency for immunofluorescent studies on the nuclei of cleavage mouse embryo. K e y w o r d s: preimplantation mouse embryos, nuclear actin, transcription, laser scanning confocal microscopy.