Permeable Supports Selection Guide Transwell Falcon
Corning w In vivo w w w in vivo Create a More Natural Environment for Your Cells Costar ready-to-use 25 Falcon Corning BioCoat Falcon Corning BioCoat Corning BioCoat Falcon PET Falcon BioCoat Falcon 2
4 1 3 PC 0.4 8.0 µm PET PET w Falcon PET w Falcon HD PET w Corning PET 400 700 nm 99% PTFE PTFE 0.4 3.0 µm 2. 0.4 1.0 µm 3.0 8.0 µm µm HMVEC HUVEC 3.0 0.4, 1.0 0.4 HUVEC HMVEC Jurkat Osteoblasts 3.0 3.0 3.0, 5.0 3.0 3.0, 5.0, 8.0 1.0, 3.0 8.0 3.0, 5.0 8.0 8.0 8.0 5.0, 8.0 8.0 5.0, 8.0 3.0, 5.0, 8.0 0.4, 3.0 Caco-2 MDCK 3.0 0.4 0.4, 1.0 0.4, 1.0 3
3 w 6 12 24 100 mm w HTS 24 96 w w 6 12 mm * cm 2 4.26 96 0.143 6.5 24 0.33 12 12 1.12 24 6 4.67 75 100 mm 44 Falcon Corning FluoroBlok Corning BioCoat mm * cm 2 3.2 96 0.08 6.4 24 0.31/0.33** 10.5 12 0.90 23.1 6 4.2 * ** 24 HTS 6 12 24 100 mm HTS 4 ECM Corning BioCoat 7 BioCoat TEL 03-3586-1268 ScientificSupportJP@corning.com 4
4 w PC 0.4 m 8.0 m w PET w Falcon PET PET 24 6.5 mm w PET Corning FluoroBlok µm 0.4 0.4 3.0 3.0 5.0 8.0 PET PC PET PC PC PC 4 10 6 1 10 8 2 10 6 2 10 6 4 10 5 1 10 5 1 6 12 24 9 9 9 9 9 9 Falcon Corning BioCoat µm 0.4 0.4 1.0 3.0 3.0 8.0 PET PET HD PET PET PET HD PET 2 10 6 1 10 8 1.6 10 6 8 10 5 2 10 6 6 10 4 1 6 12 24 10 10 10 10 10 10 Falcon BioCoat Falcon Falcon 11 5
Corning FluoroBlok Corning 400 700 nm PET Falcon Corning FluoroBlok 6.5 mm Corning FluoroBlok µm 3.0 8.0 PET PET 8 10 5 6 10 4 Corning FluoroBlok Corning FluoroBlok 24 Falcon 9 9 Snapwell 12mm Snapwell µm 0.4 0.4 Snapwell PET PC 4 10 6 1 10 8 Costar Costar 6 9 9 Snapwell Netwell Netwell µm 74 440 PET PET Netwell Costar Costar 6 12 24 9 9 6
ECM Corning BioCoat ECM Corning Caco-2 Corning Corning BioCoat BioCoat µm 0.4 1.0 3.0 3.0 8.0 PET PET PET PET I I IV GFR * 6 24 10 10 10 10 10 BioCoat µm 0.4 3.0 8.0 PET PET PET 1.6 10 6 8 10 5 1 10 5 6 24 BioCoat Falcon BioCoat ECM 11 11 11 µm 0.4 3.0 PTFE PTFE I & III 6 PTFE 1 1 12 24 11 11 7
HTS HTS Caco-2 HTS HTS µm 0.4 0.4 1.0 3.0 5.0 8.0 PET PC PET PC PC PC 4 10 6 1 10 8 1.6 10 6 2 10 6 4 10 5 1 10 5 24 96 11 13 11 13 11 13 11 13 11 13 11 13 HTS Transwell Falcon HTS µm 1.0 3.0 3.0 8.0 8.0 PET PET PET PET PET 1.6 10 6 8 10 5 8 10 5 1 10 5 1 10 5 24 96 12 13 12 12 13 12 13 12 13 HTS Falcon HTS HTS µm 0.4 1.0 3.0 8.0 PAMPA PET PET I I Corning 1.6 10 6 8 10 5 8 10 5 8 10 5 6 10 4 Gentest BioCoat BioCoat BioCoat BioCoat BioCoat Angiogenesis Angiogenesis a b C 24 96 12 11 12 11 12 11 12 11 12 11 12 a / HUVEC VEGF 1 3 µm b in vitro HMVEC-1 NIH-3T3 1 Corning 3 µm c in vitro Corning 8 µm 8
mm cm 2 m 3413 24 6.5 0.33 0.4 24 12/ * 48 20,500 3415 24 6.5 0.33 3.0 24 12/ * 48 20,500 3421 24 6.5 0.33 5.0 24 12/ * 48 20,500 3422 24 6.5 0.33 8.0 24 12/ * 48 20,500 3401 12 12 1.12 0.4 12 12/ 48 27,300 3402 12 12 1.12 3.0 12 12/ 48 27,300 3412 6 24 4.67 0.4 6 6/ 24 13,700 3414 6 24 4.67 3.0 6 6/ 24 13,700 3428 6 24 4.67 8.0 6 6/ 24 13,700 3419 75 44 0.4 1/ 12 46,400 3420 75 44 3.0 1/ 12 46,400 * 6.5 mm 24 12 1 4 3450 6 24 4.67 0.4 6 6/ 24 36,400 3452 6 24 4.67 3.0 6 6/ 24 36,400 3460 12 12 1.12 0.4 12 12/ 48 31,900 3462 12 12 1.12 3.0 12 12/ 48 31,900 3470 24 6.5 0.33 0.4 24 12/ * 48 27,300 3472 24 6.5 0.33 3.0 24 12/ * 48 27,300 3464 24 6.5 0.33 8.0 24 12/ * 48 26,500 * 6.5 mm 24 12 1 4 * mm cm 2 m 3407 6 12 1.12 0.4 PC 6 24 20,000 3801 6 12 1.12 0.4 6 24 26,000 * Harvard Apparatus www.harvardapparatus.com Corning FluoroBlok 24 351151 24 6.4 0.3 3.0 351152 24 6.4 0.3 8.0 353504 24 PET PET 1 48 30,000 1 48 30,000 1 50 30,600 mm m 3477 12 15 74 12/ 48 27,300 3478 12 15 440 12/ 48 27,300 3479 6 24 74 6/ 48 34,200 3480 6 24 440 6/ 48 34,200 3517 200 16,000 3519 200 16,000 3520 3521 15 mm 12 24 mm 6 8 10,920 8 10,920 9
Falcon PET mm cm 2 m 353095 24 6.4 0.3 0.4 PET 1 48 20,500 353104 24 6.4 0.3 1.0 PET 1 48 20,500 353096 24 6.4 0.3 3.0 PET 1 48 20,500 353180 12 10.5 0.9 0.4 PET 1 48 26,700 353103 12 10.5 0.9 1.0 PET 1 48 26,700 353181 12 10.5 0.9 3.0 PET 1 48 26,700 353090 6 23.1 4.2 0.4 PET 1 48 27,300 353102 6 23.1 4.2 1.0 PET 1 48 27,300 353091 6 23.1 4.2 3.0 PET 1 48 27,300 Falcon PET 353495 24 6.4 0.3 0.4 PET 1 48 20,500 353492 24 6.4 0.3 3.0 PET 1 48 20,500 353097 24 6.4 0.3 8.0 PET 1 48 20,500 353494 12 10.5 0.9 0.4 PET 1 48 26,700 353292 12 10.5 0.9 3.0 PET 1 48 26,700 353182 12 10.5 0.9 8.0 PET 1 48 26,700 353493 6 23.1 4.2 0.4 PET 1 48 27,300 353092 6 23.1 4.2 3.0 PET 1 48 27,300 353093 6 23.1 4.2 8.0 PET 1 48 27,300 Corning BioCoat I 354442 6 23.1 4.2 0.4 PET 6 24 35,500 354444 24 6.4 0.3 0.4 PET 12 24 28,700 354580* 6 23.1 4.2 1.0 PET 6 24 40,900 354482 24 6.4 0.3 1.0 PET 12 24 37,400 354540* 6 23.1 4.2 3.0 PET 6 24 38,300 354541* 24 6.4 0.3 3.0 PET 12 24 28,700 Corning BioCoat IV 354591* 24 6.4 0.3 1.0 PET 12 24 32,700 354544* 6 23.1 4.2 3.0 PET 6 24 47,700 354545 24 6.4 0.3 3.0 PET 12 24 32,700 Corning BioCoat 354472* 6 23.1 4.2 1.0 PET 6 24 53,200 354474 24 6.4 0.3 1.0 PET 12 24 32,700 Corning BioCoat 354440* 6 23.1 4.2 0.4 PET 6 24 42,300 354445 24 6.4 0.3 0.4 PET 12 24 32,700 354543 24 6.4 0.3 3.0 PET 12 24 32,700 Corning BioCoat FluoroBlok 354597* 24 2 6.4 0.3 3.0 PET 24 32,700 Corning BioCoat Corning BioCoat 354481 6 4 23.1 4.2 8.0 PET 6 24 54,500 354480 24 2 6.4 0.3 8.0 PET 12 24 49,200 354483 24 2 * 6.4 0.3 8.0 PET 12 24 50,400 10
Corning BioCoat Caco-2 * 355057 24 BioCoat 24 2 100 ml 1 Entero-STIM 400 ml 1 MITO 1 mm cm 2 m 6.4 0.3 1.0 PET 1 24 53,400 Corning BioCoat 353502 6 1 50 23,700 353503 12 1 50 25,000 353504 24 1 50 30,600 355467 6 1 4 5,460 Corning BioCoat 354570* 4 6 23.1 4.2 0.4 PET 6 24 27,300 354572* 2 24 6.4 0.3 0.4 PET 12 24 20,500 354567* 4 6 23.1 4.2 1.0 PET 6 24 27,300 354569* 2 24 6.4 0.3 1.0 PET 12 24 20,500 354573* 4 6 23.1 4.2 3.0 PET 6 24 17,800 354575 2 24 6.4 0.3 3.0 PET 12 24 20,500 354576* 4 6 23.1 4.2 8.0 PET 6 24 27,300 354578 2 24 6.4 0.3 8.0 PET 12 24 20,500 - mm cm 2 m 3491 6 4 24 4.67 0.4 6 1 24 37,500 3492 6 4 24 4.67 3.0 6 1 24 37,500 3493 12 2 12 1.12 0.4 12 1 24 34,100 3494 12 2 12 1.12 3.0 12 1 24 34,100 3495 24 2 6.5 0.33 0.4 24 1 24 32,700 3496 24 2 6.5 0.33 3.0 24 1 24 32,700 HTS HTS -24 mm cm 2 m 3396 24 6.5 0.33 0.4 PC 1 2 25,000 3397 24 6.5 0.33 0.4 PC 12 12 142,500 3398 24 6.5 0.33 3.0 PC 1 2 25,000 3399 24 6.5 0.33 3.0 PC 12 12 142,500 3395 12 48 19,300 3378 24 6.5 0.33 0.4 PET 12 12 136,300 3379 24 6.5 0.33 0.4 PET 1 2 25,000 Corning BioCoat HTS Caco-2 BioCoat 24 250 ml 1 Entero-STIM 250 ml 1 MITO + 0.5 ml 1 354801 BioCoat HTS Caco-2 1 / 6.4 0.3 1.0 PET 1 50,000 354802 BioCoat HTS Caco-2 5 / 4.4 0.3 1.0 PET 5 195,300 Corning BioCoat I 24 HTS 354598* HTS 24 24 6.4 0.3 3.0 PET 1 35,900 * BSE 11
Corning BioCoat I 24 mm cm 2 m 354803 6.4 0.3 1.0 PET 1 1 36,400 Corning BioCoat 354143 24 6.4 0.3 3.0 PET 1 1 53,400 354144* 24 6.4 0.3 3.0 PET 1 5 244,200 354147 96 3.2 0.08 3.0 PET 1 1 75,800 354148* 96 3.2 0.08 3.0 PET 1 5 346,100 Corning BioCoat 354141* 24 6.4 0.3 3.0 354142* 24 6.4 0.3 3.0 PET PET 1 1 55,600 1 5 252,000 Corning BioCoat 354165 24 6.4 0.3 8.0 354166 24 6.4 0.3 8.0 354167 96 3.2 0.08 8.0 354168* 96 3.2 0.08 8.0 PET PET PET PET 1 1 55,600 5 5 252,000 1 1 75,800 5 5 346,100 Corning Gentest PMAPA 353015 96 3.2 0.4 PVDF 5 5 112,500 Falcon 24 351181 6.4 0.3 1.0 PET 5 5 60,200 351183* 24 6.4 0.3 3.0 PET 5 5 60,200 351184* 24 6.4 0.3 8.0 PET 1 1 13,700 351185* 24 6.4 0.3 8.0 PET 5 5 60,200 Corning FluoroBlok 24 351155 24 6.4 0.3 3.0 351156* 24 6.4 0.3 3.0 351157 24 6.4 0.3 8.0 351158 24 6.4 0.3 8.0 PET PET PET PET 1 1 20,500 5 5 89,700 1 1 20,500 5 5 89,700 Falcon 353047 24 6.4 0.3 1 50 30,600 353226 24 6.4 0.3 6 36 22,100 353935 24 6.4 0.3 10/RS Tray** 60 35,100 353847 24 Corning 6.4 0.3 1 50 38,600 351147 24 6.4 0.3 1 50 29,200 351186 6.4 0.3 5 5 12,400 * ** RS Tray 12
HTS -96 3381 3391 7369 3380 3392 3385 3386 3387 3388 3374 3384 3382 3383 3583 3783 2 2 2 2 2 96 96 96 96 96 96 96 96 96 96 Falcon 96 mm cm 2 m 4.21 0.143 0.4 PC 1 1 32,500 4.21 0.143 0.4 PC 1 5 135,300 4.21 0.143 0.4 PET 1 5 128,800 4.21 0.143 1.0 PET 1 1 32,500 4.21 0.143 1.0 PET 1 5 135,300 4.21 0.143 3.0 PC 1 2 17,400 4.21 0.143 3.0 PC 4 8 64,900 4.21 0.143 5.0 PC 4 8 64,900 4.21 0.143 5.0 PC 1 2 17,400 4.21 0.143 8.0 PET 1 2 13,020 4.21 0.143 8.0 PET 4 8 51,900 4.21 0.143 10 10 17,400 4.21 0.143 10 10 10,820 4.21 0.143 10 10 16,300 4.21 0.143 10 10 15,800 351130 3.2 0.08 1.0 PET 1 1 36,400 351131 3.2 0.08 1.0 PET 5 5 170,400 353938 96 3.2 0.08 1.0 PET 5 5 170,400 353925 96 5 5 19,300 353924 96 5 5 17,010 Corning FluoroBlok 96 351161* 351162 351163 351164 96 96 96 96 96 96 96 96 3.2 0.08 3.0 3.2 0.08 3.0 3.2 0.08 8.0 3.2 0.08 8.0 PET PET PET PET 1 1 54,500 5 5 261,200 1 1 54,500 5 5 261,200 353928 96 5 5 19,300 * 13
Corning Life Sciences www.corning.com/lifesciences Protocols w Cell Migration, Chemotaxis and Invasion Assay Protocol CLS-AN-061 w Cell Migration, Chemotaxis and Invasion Assay Using Staining Protocol CLS-AN-211 w Fixation and Staining Procedure for Transwell Inserts Protocol w Preparation of Transwell Inserts for Histology Protocol w Trypsinization Procedure for Transwell Inserts Protocol CLS-AN-033 References and Bibliographies w Testing Cell Monolayer Integrity on Transwell Permeable Supports CLS-AN-047W Selection and Use Guide w Transwell Permeable Supports Selection and Use Guide CLS-CC-007W Technical Reports w Compatible Fluorophores and Dyes for Corning FluoroBlok Inserts and Insert Systems CLS- DL-CC-077 w Considerations when Optimizing your Chemotaxis or Invasion Assay with Corning Transwell Permeable Supports CLS-AN-188 w Corning HTS Transwell-96 Permeable Support Protocols for Drug Transport Application Note CLS-AN-058 w Design and Evaluation of an Automation-Compatible Multiwell Insert for Cell-Based Assay CLS-DL-CC-072 w In Vitro Study of Cytokine-mediated Activation of Endothelial Cell Permeability Using Falcon Cell Culture Permeable Supports CLS-DL-CC-068 w Migration of Human Mesenchymal Stem Cells using Corning FluoroBlok Inserts CLS-DL- CC-054 w New PET Membrane for Corning FluoroBlok 3.0 m and 8.0 m Pore Size Cell Culture Inserts CLS-DL-CC-042 w Preparation of Falcon Cell Culture Permeable Supports for Confocal Indirect Immunofluorescence: Fixation and Staining of Caco-2/bbe C2 Cells with Various Dyes CLS-DL- CC-079 w Screening of Anti-Metastatic Compounds by a Fluorescence-Based Tumor Cell Invasion Assay CLS-DL-CC-076 w Use of Falcon Cell Culture Permeable Supports to Reconstruct a Differentiated Human Epidermis In Vitro CLS-DL-CC-066 354801 HTS Caco-2 0.076 354802 HTS Caco-2 0.076 355057 (Caco-2) 0.076 14
Beginning-to-end Solutions for Cell Culture www.corning.com/lifesciences/solutions At Corning, cells are in our culture. In our continuous efforts to improve efficiencies and develop new tools and technologies for life science researchers, we have scientists working in Corning R&D labs across the globe, doing what you do every day. From seeding starter cultures to expanding cells for assays, our technical experts understand your challenges and your increased need for more reliable cells and cellular material. It is this expertise, plus a 160-year history of Corning innovation and manufactur ing excellence, that puts us in a unique position to offer a beginning-to-end portfolio of high-quality, reliable cell culture consumables. 2016 4 For a listing of trademarks, visit us at www.corning.com/clstrademarks All other trademarks are the property of their respective owners. 107-0052 1-11-447 Tel 03-3586-1996Fax 03-3586-1291 www.corning.com/lifesciences CLSJP@corning.com Tel: 03-3586-1268 ScientificSupportJP@corning.com 2016 Corning Incorporated CLS-085-01 R0-1604-087-T