ポリアミンVol3No2-修正 pdf
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1 Vol.3 No.2 Nov Polyamine
2 Vol. 3 No. 2 Nov Bacteroides thetaiotaomicron
3 37
4 H 2 N H 2 N H N Spermine Spermidine H 2 N N H SMO H N NH 2 NH 2 Putrescine NH 2 SSAT SSAT AcPAO AcPAO N 1 -Acetylspermine 3-Acetamidopropanal 3-Aminopropanal H 2 N H 2 N H N H N O O O N H NH 2 NHCOCH 3 NHCOCH 3 NHCOCH 3 N 1 -Acetylspermidine NHCOCH 3 3-Acetamidopropanal O Acrolein SMO, spermine oxidase AcPAO, acetylpolyamine oxidase SSAT, spermidine/spermine N 1 -acetyltransferase N N 38
5 Cell concentration (cells/ml) A Spermine, none, 30 µm SPM, 30 µm SPM+Cat 8 u r, 30 µm SPM +ALDH 30 u p, 30 µm SPM + ALDH 30 u + Cat 8 u B Hydrogen peroxide (H 2 O 2 ), none, 0.1 mm H 2 O 2, 0.2 mm H 2 O 2 r, 0.2 mm H 2 O 2 +Cat 8 u C Acrolein (CH 2 =CH-CHO), none, 7.5 µm Acrolein, 15 µm Acrolein r, 15 µm Acrolein + ALDH 30 u Time (day) N N 39
6 Molecular weight (kda) Acrolein 1D-PAGE 2D-PAGE ph (40 µm, 9 h) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) A MVKVGVNGFG RIGRLVTRAA ICSGKVEIVA INDPFIDLNY MVYMFQYDST HGKFNGTVKA ENGKLVINGK PITIFQERDP TNIKWGEAGA EYVVESTGVF TTMEKAGAHL KGGAKRVIIS APSADAPMFV MGVNHEKYDN SLKIVSNASC TTNCLAPLAK VIHDNFGIVE GLMTTVHAIT ATQKTVDGPS GKLWRDGRGA AQNIIPASTG AAKAVGKVIP ELNGKLTGMA FRVPTPNVSV VDLTCRLEKP AKYDDIKKVV KQASEGPLKG ILGYTEDQVV SCDFNSNSHS STFDAGAGIA LNDNFVKLIS WYDNEYGYSN RVVDLMAYMA SKE B アクロレイン抱合による GAPDH の核移行とアポトーシス惹起 None 4 mm Acrolein 8 mm Acrolein TUNEL Propidium iodide Merge 20 mm Western blotting of GAPDH Acrolein (8 µm, 6 h) Relative amount IP: antiacetyl-lys TUNEL positive cells (%) Acrolein (mm, 24 h) Cytoplasm - + Nucleus TUNEL
7 N ε N ε κ β 41
8 A Cell viability (%) B Cell number (x 10 4 cells/ml) Acrolein (µm) Cell viability Growth curve IC 50 Neuro2a = 4.2 µm IC 50 Neuro2a-ATD1 = 8.4 µm Neuro2a Neuro2a-ATD1 Neuro2a-ATD1 + 6 µm acrolein Neuro2a + 6 µm acrolein C Glutathione (nmol/mg protein) Glutathione 0 ** N2a N2a -ATD1 ** n.d. 200 Acrolein (µm) Relative amount kda PC-Acro N2a ATD1 N2a ATD Acrolein (µm) : Neuro2a (N2a) : Neuro2a-ATD1 (N2a-ATD1) D Cell viability (%) E Cell number (x 10 4 cells/ml) Time (Day) Cell viability Growth curve IC 50 Neuro2a = 4.2 µm IC 50 Neuro2a-ATD2 = 6.8 µm Acrolein (µm) Time (Day) Neuro2a : Neuro2a (N2a) : Neuro2a-ATD2 (N2a-ATD2) Neuro2a-ATD2 Neuro2a-ATD2 + 6 µm acrolein Neuro2a + 6 µm acrolein GSH (nmol/mg protein) F G Ratio (AcPAO/b-actin) Glutathione N2a ATD2 Western blotting (PC-Acro) kda N2a ATD2 Relative amount Levels of AcPAO and SMO mrnas 0 N2a AcPAO * ATD2 Ratio (SMO/b-actin) N2a SMO * ATD2 42
9 A vs 40 % ROC 84.1% 83.5% AUC: Relative value (AU) Control: 260 SBI: 214 CA: 263 WMH: 245 B Age/ PC-Acro/IL-6/CRP) RRV 0.14: 0.90: 0.80: 0.76: 0.65: *** *** *** *** *** β 43
10 A Control Infarct kda CBB staining Control Infarct Western blotting (PC-Acro) Control Infarct 68 Albumin Infarct area B Saline NAC Density 1 : 26*** 30 Infarct volume (mm 3 ) * 250 mg/kg, 0 Saline NAC N 44
11 N ε β 45
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16 Integrator Pump injector A Column oven Pump Detector B 50
17 N 1 -Ac MDL DE343 Ac343 N N N N N 4 7 N 8 -Ac N 1 -Ac34 Ac MDL N 1 -Ac34 DE343 N N N N N N N N N 51
18 Ac N 1 -Ac N N Integrator injector Pump A Column oven Detector Pump B 52
19 N o o N N N N N 53
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23 Aggravation of brain infarction through an increase in acrolein production and a decrease in glutathione with aging. Takeshi Uemura, Kenta Watanabe, Misaki Ishibashi, Ryotaro Saiki, Kyoshiro Kuni, Kazuhiro Nishimura, Toshihiko Toida, Keiko Kashiwagi, Kazuei Igarashi. Biochemical and Biophysical Research Communications 473 (2016) Bacteroides thetaiotaomicron Carboxyspermidine decarboxylase of the prominent intestinal microbiota species Bacteroides thetaiotaomicron is required for spermidine biosynthesis and contributes to normal growth. Mikiyasu Sakanaka, Yuta Sugiyama, Aya Kitakata, Takane Katayama, and Shin Kurihara. Amino Acids, 2016, 48: PMID: Bacteroides thetaiotaomicron casdcb. thetaiotaomicron casdccasdc casdc 57
24 Structure of the hypusinylated eukaryotic translation factor eif-5a bound to the ribosome. Christian Schmidt, Thomas Becker, André Heuer, Katharina Braunger, Vivekanandan Shanmuganathan, Markus Pech, Otto Berninghausen, Daniel N. Wilson and Roland Beckmann Nucleic Acids Research, 2016, 44: Interferon-Induced Spermidine-Spermine Acetyltransferase and Polyamine Depletion Restrict Zika and Chikungunya Viruses. Bryan C. Mounce, Enzo Z. Poirier, Gabriella Passoni, Etienne Simon-Loriere, Teresa Cesaro, Matthieu Prot, Kenneth A. Stapleford, Gonzalo Moratorio, Anavaj Sakuntabhai, Jean-Pierre Levraud, Marco Vignuzzi Cell Host Microb, 2016, 20: N 58
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ORYZA POLYAMINE ORYZA POLYAMINE -P -PC -LC 1. Ver.1.0 MM ver. 1.0MM 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504
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POLYAMINE -P Ver.1.0 MM ver. 1.0MM POLYAMINE 1. 1) Roseeuw D.I. et al., Epidermal keratinocytes actively maintain their intracellular polyamine levels. Cell Tissue Kinet. 16, 493-504 (1983). 2) Ha.C. et
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熊本大学学術リポジトリ Kumamoto University Repositor Title 炎症性腸疾患に対する HSF1 及び HSP70 の保護的役割 Author(s) 田中, 健一郎 Citation Issue date 2008-03-25 Type URL Thesis or Dissertation http://hdl.handle.net/2298/9448 Right 1
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