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- うまじ みうら
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11 4 J.R.Webster, M.A.Burms, D.T.Burke and C.H.Mastrangelo Center for Integrated Microsystems, Department of Electrical Engineering and Computer Science, Department of Chemical Engineering, and Departmentof Human Genetics, University of Michigan, Ann Arbor, Michigan Analytical Chemistry, Vol. 73, No. 7, April 1, 2001 AZO SYBR Green DNA LED SYBR-Green dsdna 0.9 cm 30, fg 7
12 Zhenhua Liang, Nghia Chiem, Gregor Ocvirk, Thompson Tang, Karl Fluri, and D. Jed Harrison Department of Chemistry, University of Alberta, Edmonton, Alberta, Canada T6G 2G2 Analytical Chemistry, Vol. 68, No. 6, March 15, 1996 U m 10 6M 20 20,000 3nM 120m 50M 0.003auS/N3 Ar 2 FITC SN3 6M 3nM 20,000 PMT 8
13 Jorg Hubner, Research Center COM, Orsteds Plads, Building 345 West, Technical University of Denmark, DK-2800 Lyngby, Denmark Klaus B. Mogensen, Anders M. Jorgensen, Peter Friis, Pieter Telleman, and Jorg P. Kutter Mikroelektronik Centret, Orsteds Plads, Building 345 East, Technical University of Denmark, DK-2800 Lyngby, Denmark REVIEW OF SCIENTIFIC INSTRUMENTS Vol.72,No.1 Jan.2001 Bodipy650665X 250pMBodipy650665X 100nM 10000m 2 50m 4 m24m m 18dB 250pM 9
14 870W 10000m 2 9W/cm 2 Bodipy 100M Michael L. Chabinyc, Daniel T. Chiu, J. Cooper McDonald, Abraham D. Stroock, James F. Christian, Arieh M. Karger, and George M. Whitesides Department of Chemistry and Chemical Biology, Harvard University, Radiation Monitoring Devices, Inc., Analytical Chemistry, Vol. 73, No.18, September 15, 2001 PDMS APDmicroavalanche photodiode PDMS 100m APD PDMS (1cm) 80m APD APD 10
15 APD 1 (30m) (50m ADP 25nM) CE APD PDMS APDs APD Mantz Webster 4 ( ) 11
16 LED 488 nm LED 75 fg 3 nm 250 pm 25 nm (DNA 72bp) 20,000 ( ) ( ) 6M 100 nm FITC (Bodipy650/655X) 12
17 cm 2 Lambert-Beer Abs. log I I 0 = εcλ I0 I ε mol/lcm -1 c mol/l λ cm Lambert-Beer 10100µm 10-3 ε=10 4 mol/l -1 cm -1 10µmλ10-3 cm c=10-4 mol/l 13
18 LIF Laser Induced Fluorescence 1 1 DNA 1 14
19 Thermal Lens Microscope Radiative Transition Non-Radiative NMR 15
20 NMR NMR CCD 16
21 CCD 3 6G 514.5nm 6465nm 100µm 1kHz 17
22 18
23 1 2σ M M M S/N= M M 2σ S/N=2 100µm 40µm GRIN GRIN 19
24 70mm30mm1.4mm 3 200µm 100µm 3 20
25 21
26 2 1 2 SELFOC R IV 23
27 24
28 S M L S M L S M L
29 SELFOC R SML SELFOC R SELFOC R SELFOC R n0 2 g r nr r 2 nr n01 g 2 2r 2 SELFOC R Z0 0Z0 2g s0cotgz0 n0g SELFOC R SELFOC R 26
30 SELFOC R 1mm 2.3mm 40um 658nm 785nm SELFOC R SELFOC R SELFOC R SELFOC R SELFOC R SELFOC R 27
31 nm 785nm 35mW ONOFF ONOFF 28
32 0 2.0kHz nm 4m 29
33 SELFOC R 14 SELFOC R 785nm 658nm SELFOC R SELFOC R 2 SELFOC R 658nm 30
34 SELFOC R SELFOC R SELFOC R 785nm SELFOC R SELFOC R SELFOC R 2 2 SELFOC R 2 SELFOC R 2 ONOFF 2.0kHz S BR 31
35 785nm 780FS mm 1mm 32
36 BNC IV NF LI5640 IV 0.01mA 3.3V IV IV IV 33
37 1 4 4 M5 X,Y X 34
38 Y Z Z Y Z Z Y X X,Y 35
39 KD Scientific Model-200 TASCO TMS-80A H 0.1 1H 2 36
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41 TTL FC FC 38
42 658nm 785nm LD 35mW 35mW FC 26mWCW mW mW 0CW 1.2kHz CW DC9V 100W140D40H 0.5Kg SELFOC R SELFOC R 1mm 2.331mm SELFOC R 658nm 785nm 40m 39
43 SELFOC R SELFOC R db 18dB 3 50dB
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48 S/N S/N M S/N S/N S/N 1kHz S/N 1kHz 46
49 S/N S/N S/N 63µW µ 47
50 2σ S/N 2 658nm 3 mol/l mol/l Abs Abs mol/l mol/l Abs Abs nm 9mW 12mW mol/l Abs mol/l Abs
51 1 3mW SELFOC NA 0.2 NA σ S/N σ S/N= mol/l mol/l
52 G 514.5nm nm ELISA γifnγ γ ELISA 25µm 50
53 IFNγ IFNγ 1µl/min µl/min5 IFN-γ HRP M a M btmbz M / 5mm 658nm IFNγ ng/ml IFNγ ng/ml 0.11ng/ml TMBZ 4-AA TOOS 0.01ng/ml 0.03ng/ml 1 IFNγ C IFN-γ (ng/ml) γ 51
54 C IFN-γ (ng/ml) γ γ 0.01ng/ml 52
55 53
56 IT MALDITOF 55
57 DNA NIST 56
58 57
59 58
60 59
61 µ µ 60
62 TEL03(3454) TEL03(3292)0642
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