Sequencher 4.9 Confidence score Clustal Clustal ClustalW Sequencher ClustalW Windows Macintosh motif confidence Sequencher V4.9 Trim Ends Without Prev

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あいりはかまや
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1 2009 Gene Codes Corporation Gene Codes Corporation 775 Technology Drive, Ann Arbor, MI USA (USA) (elsewhere) (fax)

800.497.4939 (USA) +1.734.769.7249 (elsewhere) +1.

2 Sequencher 4.9 Confidence score Clustal Clustal ClustalW Sequencher ClustalW Windows Macintosh motif confidence Sequencher V4.9 Trim Ends Without Preview. low quality 2 of 20

3 low, medium, high Contig eature map contig feature 3 of 20

4 Contig Don t show this message again GenBank Feature Feature GenBank Variance Apple Apple Sequencher 4 of 20

5 Feature Motif Sequencher 60 PHRED 100 View 2 Confidence Histogram (max 60) Confidence Histogram (max 100). ON ON 5 of 20

6 Clustal ClustalW Sequencher Larkin, et al, Bioinformatics, 2007 and Thompson, et al, Nucleic Acids Res., 1994 Nov 11. Complete documentation for the Clustal program can be found at the official Clustal web site at Sequencher 4.9 ClustalW2 version Mac OS-X clustalw macosx.dmg 2. clustalw.dmg 3. clustalw2 Macintosh 1. Windows clustalw win.msi msi 4. clustalw Sequencher [Contig] [Assemble Contigs ] [Using Clustal] 6 of 20

9 ClustalW2 version 2.0.10 1. Mac OS-X clustalw macosx.dmg http://www.clustal.org/download/current 2. clustalw.dmg 3.

7 ClustalW Clustal User Preferences Contig Clustal Clustal Options. ClustalW DNA Clustal Clustal Assemble by Name ClustalW Clustal 7 of 20

8 Sequencher Gene Codes Window Motif Definitions Highlighting ( ) Enable Highlighting 1 30 overrides others Enable Highligting, ON View Dispaly Base Confidences 8 of 20

9 Sequencher v4.9 Sequence Trim Ends Without Preview 9 of 20

10 User Preference File Import Autotrim without Preview SequencherTrimEndsHistory Microsoft Excel 10MB User Preferences File Import 1MB 1,000MB (1GB) SequencherTrimEndsHistory Mac Windows Sequencher Ends Trimming 2 1) 2) 5 10 of 20

Preferences File Import 1MB 1,000MB (1GB)

11 1 [Sequence] [Trim Ends ] Phase 1 Phase Enable phase x trim criteria of 20

12 [Select] [Next Inadequate Coverage] low quality [Select] [Next Contig Disagree] [Select] [Next Edited Base] [Select] [Next Inadequate Coverage] Select v4.9 User Preferences Confidence 12 of 20

13 [View] [Display Base Confidences] overviews restriction maps SNP 1 Discreet feature variation Discontiguous disjoint p52 13 of 20

14 SNPs 14 of 20

15 Mighty Mouse Mighty Mouse VectorNTI.ma4 15 of 20

16 V4.9 1 Sequencher Don t show this message again. User Preference Settings Reset User Preferences to Factory Defaults. FASTA Nexus Interleaved 16 of 20

17 Feature keywords GenBank V4.9 GenBank Feature, Motif User Preference page Define Feature Key Default Styles. Edit Features Sequence Mark Selection as Feature Mark Selection as Feature 17 of 20

18 Variance Macintosh AppleScript Sequencher Mac Assistive devices ON AppleScript AppleScript System Preferences Universal Access Universal Access Enable access for assistive devices 18 of 20

System Preferences Universal Access Universal

19 AppleScript Sequencher Sequencher for Mac Window Script Window ScriptEditor File Open Dictionary Sequencher of 20

20 AppleScript AppleScript Dictionary select all unincorporated fragments AppleScript ON Sequencher Trim Phase Trim Phase inclusion TRUE FALSE Trim Phase set inclusion of Trim Phase 1 to true set inclusion of Trim Phase 2 to true set inclusion of Trim Phase 3 to false Trim Parameters ON/Off 20 of 20

Sequencher Trim Phase Trim Phase inclusion TRUE FALSE Trim Phase 1 2 3 set inclusion

AJACS18_ ppt

AJACS18_ ppt 1, 1, 1, 1, 1, 1,2, 1,2, 1 1 DDBJ 2 AJACS3 2010 6 414:20-15:20 2231 DDBJ DDBJ DDBJ DDBJ NCBI (GenBank) DDBJ EBI (EMBL-Bank) GEO DDBJ Omics ARchive(DOR) ArrayExpress DTA (DDBJ Trace Archive) DRA (DDBJ