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1 PCR- (Code No. FIK-101) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4849K
2 [1] 1 [2] 2 [3] 2 [4] 2 [5] 2 [6] 4 [7] 6 [8] 7 [9] 8 0
3 [1]
4 [2] 2x PCR Master Mix 1ml x Primer Mix 1ml 1-20 UNG(Uracil-DNA Glycosylase 200 l 1-20 [3] 100bp 1.5ml 0.2mlPCR 20ml 1.5ml 95 12,000rpm MultiNA MultiNA DNA-1000 SYBR Gold Code:DNA SYBR Molecular Probes Inc. [4] inva VT1, VT2, VT2vha, VT2vhb, VT2vp1 ipah ipah [5] PCR PCR 2
5 PCR PCR dttp dutp DNA UNG UNG dutp PCR dttp DNA UNG PCR UNG UNG UNG / ( /) ipah 10 4 inva VT 10 6 InvA VT ipah InvA VT ipah 3
6 [6] A, B A 1) 1.5ml 50μl 2) 1-2 3) 10μl 50 B 1) 20ml 2.5ml 2) 2-3 3) 2-3 4) 50 5) 2-3 6) 0.5ml 1.5ml 1) 1.5ml 95 5min 2) 12,000rpm X 3 3) 2μl PCR 4 PCR PCR 1) 2) 3) 4) 18μl 5.6 μl 2x PCR Master Mix 10.0 μl 10x Primer Mix 2.0 μl UNG 0.4 μl 2.0 μl 20.0 μl 4
7 PCR UNG : 20, 10 95, 2 : 94, 10 : 57, : 68, 30 : 94, 10 : 55, : 68, 30 : 94, 10 : 53, : 68, MultiNA PCR DNA 100bp DNA Ladder DNA MultiNA bp 5 mv bp 7 mv bp 2mV bp 2 mv 5
8 (1) 2 (2) [7] 6
9 [8] O O157 cfu/g) 0 7x10 2 7x10 3 7x10 4 7x10 5 7x10 6 PCR cfu/g) 0 7 x x x x x10 6 PCR cfu/g) 0 4 x x x x x10 6 PCR 50 O157 cfu/g) x10 2 7x10 3 7x10 4 7x10 2 7x10 3 7x x x x10 4 7
10 [9] 10 UV inva, VT, ipah 10 UV 8
11 9
12 () TEL FAX order_lifescience@toyobo.jp () TEL FAX order_lifescience@toyobo.jp TEL FAX :00 12:00, 13:00 17:00 () tech_osaka@toyobo.jp URL
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K
17-05 THUNDERBIRD SYBR qpcr Mix (Code No. QPS-201, QPS-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A4196K - 18 - [1] 1 [2] 2 [3] 3 [4] 5 [5] : RNA cdna 13 [6] 14 [7] 17 LightCycler TM Idaho
More information(2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene (9) 1 RT-PCR (10) (12) (13) F. Hoffmann-La Roche Ltd. Roche Molecular
05-8 SYBR Green Realtime PCR Master Mix (Code No. QPK-201, QPK-201T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3314K (2) (2) (3) (3) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux
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06-08 SYBR Green Realtime PCR Master Mix -Plus- (Code No. QPK-211, 211T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN (2) (3) (3) (4) (5) ABI PRISM 7700 (5) Roche LightCycler TM (7) BioFlux LineGene
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05-8 Realtime PCR Master Mix (Code No. QPK-101, QPK-101T) TOYOBO CO., LTD. Life Science Department OSAKA JAPAN A3313K (2) (2) (3) (3) (4) ABI PRISM 7700 TaqMan (4) Roche LightCycler TM (5) BioFlux LineGene
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27 194120331 JAPAN ASIA INVESTMENT CO., LTD. P01 P03 P05 P07 P08 P09 P10 P11 P13 P14 2,347 232 01 02 03 04 05 06 07 08 09 10 11 20,051 5,550 4,796 3,049 88,123 10,750 13,521 30,190 55,162 21,444 5,172
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