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1 StepOnePlus / StepOne PCR

2 PCR Applied Biosystems StepOnePlus StepOne PCR 1

3 Features At A Glance StepOne StepOnePlus / FAM/SYBR Green dyes VIC/JOE dyes ROX dyes NED/TAMRA dyes 1. VeriFlex Block StepOnePlus StepOne PCR 48 StepOne 96 StepOnePlus 1 PCR 1 PCR PCR log 20 μl 10 RNase P StepOne StepOne 96 StepOne StepOnePlus PCR StepOne StepOnePlus 3 48 StepOne 4 96 StepOnePlus PCR LED FAM SYBR Green VIC JOE ROX SNP / StepOnePlus TAMRA 40 Fast PCR LCD USB E StepOnePlus VeriFlex PCR 6 StepOne StepOnePlus 2

4 StepOnePlus StepOne USB LAN Local Area Network E E StepOnePlus StepOne 5 LAN LAN LAN 3 4. StepOne 5

5 StepOnePlus StepOne PCR 5 4 CT PCR SNP E & PowerPoint Excel jpeg 5. StepOne Multiple Plots 1 4 Multiple Plots SNP 6. StepOne PCR StepOne LAN 4

6 StepOnePlusStepOne FAMVIC Applied Biosystems TaqMan MGB VIC TAMRA Applied Biosystems SYBR Green I StepOnePlus TAMRATaqMan MGB SYBR Green I 1 StepOnePlus StepOne CT CT StepOnePlus StepOne SNP microrna StepOnePlus StepOne Fast 2 Fast 40 PCR Fast 10 Amplification Plot Allele Discrimination Plot (SNP Assay: SNP Assay 1) ΔRn 1 Allele Cycle Allele 1 Homozygous 1/1 Heterozygous 1/2 Homozygous 2/2 Undeterminded 5 7. PCR StepOne 10 18S rrna TaqMan 10 log 8. SNP CYP2C19*2 TaqMan SNP Genotyping Assays StepOnePlus

7 VeriFlex 96 StepOnePlusPCR 6 VeriFlex VeriFlex PCR PCR VeriFlex PCR 6 VeriFlex VeriFlex 96 PCR VeriFlex StepOnePlus StepOne PCR StepOnePlus StepOne5,000 10,000 DNA 99.7 StepOnePlus StepOne 7 10 log 3.4 Melt Curve 10 Multiplex Assays 2.9 Derivative Reporter (-Rn ) ΔRn Tm: 79.2 Temperature ( C) Cycle 9. PCR 18S GAPDH 3 Tm 10. StepOnePlus TaqMan 18S rrna VIC TGF- FAM cdna 48 6

8 2 < height: 51.8 cm > < depth: 48.5 cm > height: 51.8 cm > < depth: 48.5 cm > < < > width: 24.6 cm < > width: 24.6 cm StepOne 48 3 StepOnePlus StepOnePlus 96 4 VeriFlex 7

9 PCR / StepOnePlus StepOne PCR PCR Ambion Applied Biosystems RNA RNA Invitrogen RNA StepOnePlus StepOne PCR 1 StepOnePlus StepOne StepOne StepOnePlus TaqMan MicroRNA Assays microrna TaqMan Applied Biosystems TaqMan MicroRNA Assays MicroRNA Human MouseRatArabidopsis Drosophila C. elegans www. thermofisher.com/taqman TaqMan SNP Genotyping Assays Applied Biosystems TaqMan SNP Genotyping Assays HapMap SNPs 70,000 csnps 160,000 2,500 Drug Metabolism Genotyping Assays Custom TaqMan SNP Genotyping Assays SNP TaqMan SNP Genotyping Assays Primer Express Applied Biosystems Primer Express v3.0 TaqMan PCR SYBR Green I Primer Express StepOnePlus StepOne 8

10 TaqMan Gene Expression Assays 700,000 PCR Applied Biosystems TaqMan Gene Expression Assays TaqMan Assays PCR PCR Custom TaqMan Gene Expression Assays TaqMan Gene Expression Assays TaqMan Gene Expression, SNP Genotyping, and MicroRNA Assays TaqMan Assays Gene Expression* SNP Genotyping MicroRNA TaqMan Yes Yes Yes Inventoried Made-to-Order Custom TaqMan Assays Yes Yes No Human > 2,024,000 > 4,000,000 > 300 Mouse > 179,000 > 10,000 > 240 Rat > 128,000 > 180 Drosophila melanogaster > 38,000 > 50 Arabidopsis thaliana > 95,000 > 40 C. elegans > 90,000 > 60 Canine > 6,000 N/A Rhesus macaque > 70 N/A * mirna Gene copy number Mitochondrial HapMap drug metabolism genotyping SNPTaqMan 11. 9

11 TaqMan Fast Advanced Master Mix TaqMan Universal PCR Master Mix Power SYBR Green PCR Master Mix 96 StepOnePlus/StepOne PCR Material List 11 StepOnePlus/StepOne PCR MicroAmp 48-Well Optical Adhesive Film MicroAmp 48-Well Optical Adhesive Film MicroAmp 96-Well Optical Adhesive Film MicroAmp 96-Well Optical Adhesive Film MicroAmp Fast Optical 48-Well Reaction Plate MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) MicroAmp Fast Optical 96-Well Reaction Plate with Barcode (0.1 ml) MicroAmp Fast 8-Tube Strip, 0.1 ml MicroAmp Optical 8-Cap Strip MicroAmp Fast 48-Well Tray MicroAmp 96-Well Tray for Veriflex Blocks MicroAmp 48-Well Base Adaptor Power SYBR Green PCR Master Mix 5mL TaqMan Gene Expression Master Mix 5mL TaqMan Genotyping Master Mix 10mL TaqMan Fast Advanced Master Mix 5mL TaqMan Fast Virus 1-Step Master Mix SuperScript VILO cdna Synthesis Kit

12 Ordering information StepOne PCR StepOnePlus PCR StepOne-01 StepOnePlus-01 PCR For Research Use only.not for use in diagnostic procedures Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems. Printed in Japan. SDS024-E1602OB TEL FAX TEL FAX facebook.com/thermofisherjapan

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