To establish a local therapy of periodontitis by using minocycline HCl, a series of studies has been conducted. The present study was to re-confirm th

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1 Local Administration of Minocycline for Periodontitis Double Blind Comparative Study of LS-007 Yoji MURAYAMA1), Yoshio NOMURA1), Akira YAMAOKA2), Masatoshi UEDA2), Toshio HORP), Masato MINABE3), Toshio UMEMOTO4), Isao ISHIKAWA5), Ryoji URAGUCHP), Kazue UENO6), Kunitomo WATANABE6) and Nobuya OGAWA7) 1) Department of Periodontology and Endodontology, Okayama University Dental School Chief: Prof. Yoji MURAYAMA) ( 2) Department of Periodontology, Osaka Dental University Chief: Prof. Akira YAMAOKA) ( 3) Department of Periodontology, Kanagawa Dental College Chief: Prof. Toshio HORI) ( 4) Department of Oral Microbiology, Kanagawa Dental College Chief: Prof. Toshio UMEMOTO) ( 5) Department of Periodontology, School of Dentistry, Tokyo Medical and Dental University Chief: Prof. Isao ISHIKAWA) ( 6) Institute of Anaerobic Bacteriology, Gifu University School of Medicine Chief: Prof. Kazue UENO) ( 7) Deptartment of Pharmacology, Ehime University School of Medicine Chief: Prof. Nobuya OGAWA) (

2 To establish a local therapy of periodontitis by using minocycline HCl, a series of studies has been conducted. The present study was to re-confirm the results of the previous investigations, and objectively evaluate the usefulness of minocycline HCl ointment, LS-007. In this double blind study the ointment base of LS-007 (PL) was used as a control. The change of bacteria in periodontal pockets after administration of LS-007 was also examined. Results are stated below. 1. The LS-007 group showed significant superiority to the PL group in clinical effectiveness, overall efficacy, usefulness, improvement rate, and usefulness rate. 2. In the improvement of clinical signs (probing depth and bleeding on probing) and in the antibacterial effect the LS-007 group showed significant superiority to the PL group. 3. The LS-007 was significantly superior to the PL in decreasing isolated bacterial species. Also, the LS-007 group showed a significant reduction in a number of isolated bacteria, number of blackpigmented Bacteroides spp., and the proportion of black-pigmented Bacteroids spp. in the total isolated bacteria. 4. Noted as side effect was subjective irritation at drug injection in one tooth each of the two groups. From the results it was concluded that LS-007 was a useful drug for the local treatment of periodontitis. Key words: Periodontitis, Local antibiotic therapy, Minocycline HCl, LS-00-, LS-007 ointment base

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15 5) Walter, C. B., Ratliff, D., Muller, D., Mandell, R. and Socransky, S. S.: Medium for selective isolation of Fusobacterium nucleatum from hu- periodontal pockets. man J. Clin. Microbiol., 10: , ) Slots, J.: Selective medium for isolation of Actinobacillus actinomycetemcomitans. J. Clin. Microbiol., 15: , 1982.

16 7) Mishimo, P. A., Yamamoto, Y., Nakamura, M. periodontal disease: Virulence factors in colonization, and Slot, J.: Selective recovery of oral Capnocytophaga survival, and tissue destruction. J. spp. with sheep blood agar containing Dent. Res., 63: , bacitracin and polimyxin B. J. Clin. Microbiol., 18) Slots, J.: Importance of black-pigmented Bacteroides 17: , in human periodontal disease, Genco, R. J. and Mergenhagen, S. E. Eds., Host-Parasite Interactions in Periodontal Diseases, Amer- ican Society for Microbiology, Washington, D. C., 1982, ) Zambon, J. J., Christersson, L. A. and Slots, J.: Actinobacillus actinomycetemcomitans in human periodontal disease. Prevalence in patient groups and distribution of biotypes and serotypes within families. J. Periodontol., 54: , ) Newman, M. G. and Socransky, S. S.: Predominant cultivable microbiota in periodontosis. J. Periodont. Res., 12: , ) Loesche, W. J., Syed, S. A., Laughon, B. G. and Stoll, J.: The bacteriology of acute necrotizing ulcerative gingivitis. J. Periodontol., 53: ) Ciancio, S. G., Slots, J., Reynolds, H. S., Zambon, 230, J. J. and McKenna, J. D.: The effect of shortterm 22) Tanner, A. C. R., Haffer, C., Bratthall, G. T., administration of minocycline HCl on gin- Visconti, R. A. and Socransky, S. S.: A study of gival inflammation and subgingival microflora. the bacteria associated with advancing periodontitis J. Periodontol., 53: , in man. J. Clin. Periodontol., 6: ) Ciancio, S. G., Mather, M. L., and McMullen, 307, J. A.: An evaluation of minocycline in patients 23) Mashimo, P. A., Yamamoto, Y., Slots, J., Park, with periodontal disease. J. Periodontol., 51: B. H. and Genco, R. J.: The periodontal microflora , of juvenile diabetics : Culture, immuno- 14) Listgarten, M. A., Lindhe, J. and Hellden, L.: fluorescence and serum antibody studies. J. Effect of tetracycline and/or scaling on human Periodontol., 54: , periodontal disease. J. Clin. Periodontol., 5: 24) Savit, E. D. and Socransky, S. S.: Distribution , of certain subgingival microbial species in 15) Lindhe, J., Heul, L., Goodson, J. M. and selected periodontal conditions. J. Periodont. Socransky, S. S.: Local tetracycline delivery Res., 19: , using hollow fiber devices in periodntal therapy. J. Clin. Periodontol., 6: , ) Slots, J.: Subgingival microflora and periodontal disease. J. Clin. Periodontol., 6: , 26) Wolff, L. F., Bakdash, M. B. and Bandt, C. L.: Microbial interpretation of plaque relative to 17) Slots, J. and Genco, R. J.: Black-pigmented the diagnosis and treatment of periodontal Bacteroides species, Capnocytophaga species, and disease. J. Periodontol., 56: , Actinobacillus actinomycetemcomitans in human 27) Slots, J.: The predominant cultivable organ-

17 isms in juvenile periodontitis. Scand. J. Dent. Res., 84: 1-10, ) Slots, J.: The predominant cultivable microflora of advanced periodontitis. Scand. J. Dent.

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