Table 1 Detection bacterial strains used in this study *1; Methicillin resistant strains(mrsa)
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1 Key words: PCR, ribosomal RNA, bacterial contamination
2 Table 1 Detection bacterial strains used in this study *1; Methicillin resistant strains(mrsa)
3 PCR法 Fig. 1 Agarose gel ribosomal DNA Amplification primers (rdna) from using ethidium s are lar markers as resistant Typhi, 4; 6; Verotoxin (VTEC), 7; (EIEC), 10: C. in A, producing 3; each 5; mixed, E. coli 8; E. rdna coli A. Mlul, Eco molecular 所 の み で 切 断 さ れ,MluIで 541と347bp,EcoRIで 3; RI 1% A (E). on to E indi- type-1 (C), Lanes 0; individual to agarose parahaemolyticus MRSA HindlIl, 37 Ž. used dysenteriae performed marker Panels PCR- at strains, respectively. s (0./HindIII P. *, ) は は732と156bp,HindIIIで は758と130bpの る.そ PCR 1 to 1ƒÊl 60min TenƒÊl mixture fragments (B), (D) s water for metnod. sobria follows. 5 `10units) PCR- 類 の 制 限 酵 素 で 処 理 す る と,い HindIII. concentrated distilled 10ƒÊl or ten-fold incubated gel- as (about incubation, (A), ず れ の 場 合 も1ヵ 16ƒÊl aeruginosa で は 使 用 し た3種 3ƒÊl buffer, cates digested EcoRI performed product, confirm jciuni. target separately Mlul, endonuclease After endonuclease 2; endonuclease. PCR fragments amplified gel (888bp) Enteroinvasive Agarose restriction each (MRSA), parahaemolyticus, 9; An molecu- ), Paratyphi 2 restriction 11; 497 rdna agarose-gel 1 aureus cholerae, Typhimurium, 1% samples : s (ƒé/hindiii methicillin Fig. observed follows PCR- detection microorganisms. staining bromide. for に よ る細 菌汚 染 確 認 小 断片 とな る こ とがわ か って い こで そ れ らの 制 限 酵 素 の 認識 部 位 の有 無 に よ る 群 別 で は,全 て の 酵 素 に 感 受 性 のEcol4 Shigella spp.のi群,上 記2菌 EcoRIに 感 受 性 の あ るSalmonellaを るEnterobacteriaceae科 のII群,Mlulの 種 以 外 のMluI, は じめ とす お よ びAeromonas み で 切 断 さ れ るVibrio sppspp.とp. damselaのiii群,p.aeruginosa,c.jeiuni,c.fetus で み ら れ る よ う な3種 類 さ れ た.な 類 に 感 受 性 が な いIV群 おC.fetusで 合 に は,切 断 前rDNAサ はMlulで グ ラ ム 陽 性 菌 のMRSAで は み ら れ な か っ た. は グ ラ ム陰 性 菌 と同 じ 増 幅 さ れ た が,MluIに 受 性 が な く,EcoRI,HindIIIに I IV群 切 断 した 場 片 中 に は,HindIIIに2ヵ 認 識 部 位 を 持 つ こ と を 意 味 す る3断 平 成6年4月20日 は感 は 感 受 性 が あ り, と は 異 な っ た パ タ ー ン(V群)を ま た こ のrDNA断 I群 のE.coliやShigella 示 し た. 所 の 片 が 確 認 され spp-の 塩 基 配 列 とは 違 っ た も の で あ っ た(Fig.2). イ ズ よ り小 さ く な っ た 泳 動 像 が み ら れ た がC.jejuniで サ イ ズ の 標 的DNAが に分 考 察 細 菌 の タ ン パ ク 合 成 に 関 与 す る リボ ソ ー ム は, 近年 系統 発 生的 研 究 のた め そ の構造 解 析 が行 わ れ1),そ れ を 構 成 し て い るrRNAは,DNAに 比べ て 細 菌 細 胞 内 に 大 量 に 存 在 し て い る こ と か ら, PCRを 行 う に はDNAを 対 象 とす る よ り も 効 率 良 く実 施 で き る.rRNAに の3種 が 知 ら れ て い る が,そ は5S,16Sと23SRNA の 大 き さ か ら16Sが
4 Table 2 Restriction endonuclease patterns groups bacteria examined Verotoxin-producing E. coli; 2, Enteroinvasive E. coli; 1 Enteropathogenic E. coli; 1 strains examined. dysenteriae 1; flexneri 3a; 1 sonnei; 2 strains examined. Typhi; 2, Paratyphi A; 2, Enteritidis; 1 Typhimurium; 2 strains examined. C. freundii; 2 C. diversus; 5 strains examined. P. mirabilis; 1 P. vulgaris; 1 strains examined. K. pneumoniae; 2 K. oxytoca; 1 strains examined. A. hydrophila; 2 A. sobria; 1 strains examined. cholerae O1, strain 569B included. most clear DNA fragment this enzyme recognized a small fragment. This result not observed in case C. jejuni strains. two DNA fragments Hind III endonuclease different from its group I.
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Bull. NARO. Food. ResNo., 研究ノート 3M TM 病原菌自動検出システム改良サルモネラ検出キット MDA2 SAL の評価 Fia Noviyanti - -- - -- - - - - Evaluation of 3M TM Molecular Detection System MDA2 SAL for the detection of Salmonella from food
〈企業特集：検査機器・試薬・技術の新たな展開〉新規マイコプラズマ抗原検査キット—プロラスト®Myco
New immunochromatographic assay kit for Mycoplasma pneumoniae infection 'PRORAST Myco' Shohei Ohshima, Yasushi Shimada, Atsuko Minagawa, Kazuyuki Sugiyama and Yumiko Hirayama Mycoplasma pneumoniae infection
1 Fig. 1 Extraction of motion,.,,, 4,,, 3., 1, 2. 2.,. CHLAC,. 2.1,. (256 ).,., CHLAC. CHLAC, HLAC. 2.3 (HLAC ) r,.,. HLAC. N. 2 HLAC Fig. 2
CHLAC 1 2 3 3,. (CHLAC), 1).,.,, CHLAC,.,. Suspicious Behavior Detection based on CHLAC Method Hideaki Imanishi, 1 Toyohiro Hayashi, 2 Shuichi Enokida 3 and Toshiaki Ejima 3 We have proposed a method for
Fig.1 Chemical structure of BAY o 9867
Fig.1 Chemical structure of BAY o 9867 CHEMOTHERAPY 43 Table 3 Antibacterial spectrum of gram negative bacteria Medium:Heart infusion agar (Nissui) Method:Agar dilution (Streak) CHEMOTHERAPY DEC 1985
SUMMARY Physical effects of sampling conditions and preservation time on the nature of mitochondrial DNA (mtdna) were studied by analyzing electrophor
SUMMARY Physical effects sampling conditions preservation time on the nature mitochondrial DNA (mtdna) were studied by analyzing electrophoretic patterns measuring volume purity mtdna which was fractionated
日本化学療法学会雑誌第64巻第4号
β β Moraxella catarrhalisescherichia colicitrobacter Klebsiella pneumoniaeenterobacter cloacaeserratia marcescens Proteus Providencia Pseudomonas aeruginosaacinetobacter Bacteroides fragilis β β E. colik.
CHEMOTHERAPY Methicillin-resistant S.aureus(MRSA) coccus epidermidis 105 Streptococcus pyogenes E.faecali senterococcus avium Enterococcus faecium Str
cefaclor(ccl),cefuroxime(cxm),cefixime (CFIX),cefteram(CFTM),cefdinir(CFDN) pneumoniae,streptococcus pyogenes Moraxella catarrhalis,haemophilus influenzae,escherichia coli, Klebsiella pneumoniae,proteus
CHEMOTHERAPY APRIL 1992 Table 2. Concentration of meropenem in human prostatic fluid Table 1. Background of 21 chronic complicated UTI cases * NB + BPH, NB + Kidney tumor, NB + Kidney tuberculosis Table
Key words: Disinfectants, Gram negative rods, Bactericidal effect P. aeruginosa 1, P. fluorescens 20 P. putida 179, P. cepacia 216 P. maltophilia 227,
Key words: Disinfectants, Gram negative rods, Bactericidal effect P. aeruginosa 1, P. fluorescens 20 P. putida 179, P. cepacia 216 P. maltophilia 227, P. putrefaciens 279 A. faecalis 120, A. ordrans 114
A Nutritional Study of Anemia in Pregnancy Hematologic Characteristics in Pregnancy (Part 1) Keizo Shiraki, Fumiko Hisaoka Department of Nutrition, Sc
A Nutritional Study of Anemia in Pregnancy Hematologic Characteristics in Pregnancy (Part 1) Keizo Shiraki, Fumiko Hisaoka Department of Nutrition, School of Medicine, Tokushima University, Tokushima Fetal
VOL. 33 S-5 CHEMO THERAPY Fig. 1 Chemical structure of HAPA-B 1-N-[ (2 S)-3-Amino-2-hydroxypropiony1]-4- O-(6-amino-6 - deoxy-ƒ -D- glucopyranosyl) -6
![VOL. 33 S-5 CHEMO THERAPY Fig. 1 Chemical structure of HAPA-B 1-N-[ (2 S)-3-Amino-2-hydroxypropiony1]-4- O-(6-amino-6 - deoxy-ƒ -D- glucopyranosyl) -6](/thumbs/101/149863612.jpg "VOL. 33 S-5 CHEMO THERAPY Fig. 1 Chemical structure of HAPA-B 1-N-[ (2 S)-3-Amino-2-hydroxypropiony1]-4- O-(6-amino-6 - deoxy-ƒ -D- glucopyranosyl) -6")
VOL. 33 S-5 CHEMO THERAPY Fig. 1 Chemical structure of HAPA-B 1-N-[ (2 S)-3-Amino-2-hydroxypropiony1]-4- O-(6-amino-6 - deoxy-ƒ -D- glucopyranosyl) -6- O-[3-deoxy-4 -C-methyl-3 -(methylamino) -ƒà-l- arabinopyranosyl]-2
Table 1 Sensitivity distribution of clinical isolates 1. Escherichia coli Inoculum size: 106cells/ml 2. Klebsiella pneumoniae 3. Enterobacter cloacae 4. Serratia marcescens Inoculum size: 106cells/nil
44 4 I (1) ( ) (10 15 ) ( 17 ) ( 3 1 ) (2)
(1) I 44 II 45 III 47 IV 52 44 4 I (1) ( ) 1945 8 9 (10 15 ) ( 17 ) ( 3 1 ) (2) 45 II 1 (3) 511 ( 451 1 ) ( ) 365 1 2 512 1 2 365 1 2 363 2 ( ) 3 ( ) ( 451 2 ( 314 1 ) ( 339 1 4 ) 337 2 3 ) 363 (4) 46
i ii i iii iv 1 3 3 10 14 17 17 18 22 23 28 29 31 36 37 39 40 43 48 59 70 75 75 77 90 95 102 107 109 110 118 125 128 130 132 134 48 43 43 51 52 61 61 64 62 124 70 58 3 10 17 29 78 82 85 102 95 109 iii
16_.....E...._.I.v2006
55 1 18 Bull. Nara Univ. Educ., Vol. 55, No.1 (Cult. & Soc.), 2006 165 2002 * 18 Collaboration Between a School Athletic Club and a Community Sports Club A Case Study of SOLESTRELLA NARA 2002 Rie TAKAMURA
TEL:03-5253-1111 2478 TEL:03-3595-2337 FAX:03-3503-7964 Shigella sonnei 200g 300 500cm 0.2 0.3mm 25g 0.45 m Shigella sonnei 3mL 35 37 18 ml 0.1 ml 10 10 ml 40mL 0.1 200 L 25g 1 225mL 35 37 18 24 Shigella
CHEMOTHERAPY JUNE 1986
VOL. 34 S-3 CHEMOTHERAPY Fig. 1 Structural formula of L-105 CHEMOTHERAPY JUNE 1986 VOL. 34 S-3 CHEMOTHERAPY Table 1 Antibacterial spectra of L-105 against gram negative anaerobic rods Inoculum 106 cells/ml
Table 1 Plasmid profiles, drugs resistant and phage types of S. Enteritidis food poisoning and egg solution *: in Kumamoto City.
Key words: Salmonella Enteritidis, tetracycline resistance Table 1 Plasmid profiles, drugs resistant and phage types of S. Enteritidis food poisoning and egg solution *: in Kumamoto City. 1354 Table 本
2種の(1→3)-β-D-グルカン測定試薬の真菌に対する反応性の比較
Comparison of the reactivity of two kinds of (1 3)- -D-glucan measurement reagents to fungi Tadashi Matsubayashi and Tomoyuki Yamazaki Measurements of (1 3)- -D-glucan are widely used for the diagnosis
2 1 ( ) 2 ( ) i
21 Perceptual relation bettween shadow, reflectance and luminance under aambiguous illuminations. 1100302 2010 3 1 2 1 ( ) 2 ( ) i Abstract Perceptual relation bettween shadow, reflectance and luminance
b) Gram-negative bacteria Fig. 2 Sensitivity distribution of clinical isolates : E. coli Fig. 3 Sensitivity distribution of clinical isolates : Pseudomonas Fig. 1 Sensitivity distribution of clinical isolates
Key words: disinfectant, amphoteric surfactant, ethanol, Mycobacterium tuberculosis Table 1 Bactericidal effects of cresol soap and an amphoteric surfactant (ADG) against M. tuberculosis H37Rv : The numbers
E. coli E. coli é H. influenzae Helicobacter pylori
é C.pneumoniae C.trachomatis C.trachomatis 1 é Chlamydia pneumoniae Chlamydia psittaci Chlamydia trachomatis 3 E. coli E. coli é H. influenzae Helicobacter pylori K. pneumoniae L. monocytogenes ü l Staphylococcus
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20 Individual Recognition using positions of facial parts 1115081 2009 3 5 ,,,,,,,,,,,,,,,,,,, 976%, i Abstract Individual Recognition using positions of facial parts YOSHIHIRO Arisawa A facial recognition
400 46 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 Dec. 2012 LVFX 100 mg 3 / 7 150 mg 2 / 7 2 2006 2008 9 LVFX PK PD 2009 7 100 mg 1 3 500 mg 1 1 AUC/MIC
Dec. 2012 THE JAPANESE JOURNAL OF ANTIBIOTICS 65 6 399 45 2012 11 5 LVFX 500 mg 1 1 20 Chlamydia trachomatis C. trachomatismycoplasma genitalium M. genitalium LVFX 1 500 mg 1 1 7 22 22 C. trachomatis 17
Table 1 Results of acid-fast bacilli contamination within endoscopes and endoscope washers * The species of bacteria were not clarified of other bacte
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Jpn.J.Leprosy 67, (1998)
Jpn.J.Leprosy 67,287-291(1998) Fig.1. Macroscopic appearance of the cultured slides stained by the Ziehl-Neelsen method. Up to bottom: kept at 4 C, 18 weeks, cultured for 6 weeks, 8 weeks, 10 weeks, 12
DISTRIBUTION OF NEUROPEPTIDES IN THE INFERIOR NASAL TURBINATE MUCOSA OF PATIENTS WITH ALLERGIC RHINITIS KAZUHIRO YAMAMOTO. M.D. Department of Otolaryngology, School of Medicine, Kitasato University, Sagamihara
VOL.48 NO.7 lase negative staphylococci, Escherichia coli, Klebsiella spp., Citrobacter freundii, Enterobacter spp., indole-positive Proteus, Serratia
ceftazidime, cefpirome, cefepime, cefoperazone/sulbactam (2: 1), imipenem Staphylococcus aureus oxacillin coagulase negative staphylococci Escherichia coli piperacillin Klebsiellac spp. Citrobacter Pseudomonas
A study about the role of improvisation during the working process as to make a contemporary dance piece Keywords: improvisation, creation, contemporary dance Hidemi SOMA and Eriko HOSOKAWA The theme of
Key words: Staphylococcus epidermidis, biotype, slim production, fi-lactamase test, plasmid analysis
Key words: Staphylococcus epidermidis, biotype, slim production, fi-lactamase test, plasmid analysis 臨 床 材 料 由 来 のS.epidermidisに し た.血 液 由 来株 以 外 の 臨 床 材 料 由来 株 は と くに 生 化 学 的 性 状(生 APIス タ フ(ア ス カ 純 薬)とVITEK(マ
Table 1 Characteristics of the study participants in Imari municipal hospital
Key words: tuberculosis, booster phenomenon, two-step tuberculin test Table 1 Characteristics of the study participants in Imari municipal hospital Fig. 1 Frequency distributions of size of the first tuberculin