48 皮 膚 第29巻 第1号 昭 和62年2月 第1-b図 第1-a図 第1図Micro Trak法 アッ 皮 細 胞 中あ るいは細 pin point sizeの による 胞 外に,アッ 粒子と 直 接 塗 抹 標本から プル グ リ ー ン に光 プル し てelelnentary る 粒子
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1 Chlamydia trachomatis-syphilis-micro Trak Mariko SEISHIMA, M. D., Satoshi OKUMURA, M. D. and Michihiro OHTANI, M. D. Megumi WATANABE, Ph. D., Ryo MATSUMOTO, Ph. D., Masayoshi TSUTSUMI, Ph. D. and Kazumasa HIKICHI, Ph. D. Special Reference Laboratories
2 48 皮 膚 第29巻 第1号 昭 和62年2月 第1-b図 第1-a図 第1図Micro Trak法 アッ 皮 細 胞 中あ るいは細 pin point sizeの による 胞 外に,アッ 粒子と 直 接 塗 抹 標本から プル グ リ ー ン に光 プル し てelelnentary る 粒子と して のC.trachomatisの 観察さ れ る(矢 内 服 を1週 bodyが 陽 性 例 が あった が,1か 月 後 に,Micro 検査 を 行っ たと 例 でC.trachomatisを 観察 さ 男性 梅毒 患 者 なかっ 9例 に おい て10検体 を 採 取 し た が,そのうち1検 て陽性 を 示 した これは 症 例 数 と し ては11.1%の 数として は10%の 陽性 率であっ 間行っ た 内 服 終了 直 後 の 検 査で は1例 ころ,全 二期梅 毒 患 者2例で 陽 性率 であり,検 た この で陽 であっ た 女 性 梅毒 患 者24例に (第1表,第2表) た 男 女 合わ せ て9例 あっ た つ 症 例 数と し て は33.3%の 体 数 と して は23.5%の な お,C.trachomatisが による 検出 し た 陽 性率 い て511検体 を 採 取 した が,そ の う ち8症 例12検 体 に お い て 陽 性であっ た 8症 例の 内 訳は 第 一期梅毒 患 者6例, 第 Trak法 の み 体の み 第1表 梅 毒 患 者 におけ の 検出(Micro 性 例 は 第一 期 梅毒 患者 bodyが グ リー ン に 光る れ た標 本 を 陽性 と した(第1-a,b図) あ り,検体 検出 elementary 印) 陽性 率であっ た 検出 され につ いて は ミ ノマ イシ ン(R)200mg/日 るChlamydia trachomatis Trak法)
3
4 8. Stephens, R. S., Tam, M. R., Kuo, C. C. and Nowinski, R. C.: Monoclonal antibodies of Chla- mydia trachomatis : antibody specificities and antigen characterization, J. Immunol., 128 : , Thompson, S. E. and Washington, A. E. : Epidemiology of sexually transmitted Chlamydia trachomatis infections, Epid. Rev. 5: , Taylor-Robinson, D. and Thomas, B. J. : The role of Chlamydia trachomatis in genital-tract and associated diseases, J. Clin. Pathol., 33 : Oriel, J. D. and Ridgway, G. L.: Comparison of 233, tetracycline and minocycline in the treatment of nongonococcal urethritis, Br. J. Vener. Dis., 59 : , Center for Disease Control : Sexually transmitted diseases treatment guidelines 1982, MMWR, 31, Na2 Suppl., Grayston, J. I. and Wang, S. P.: New Knowledge of Chlamydiae and the diseases they cause, J. Infectious Diseases, 132 : , Schachter, J.: Chlamydial infections, N. Engl. J. Med., 298 : , Schachter, J.: Chlamydial infections, N. Engl. J. Med., 298 : , 1978.
5 Detection of Chlamydia trachomatis from Patients with Primary and Secondary Syphilis Mariko Seishima, Satoshi Okumura and Michihiro Ohtani Department of Dermatology, Gifu Prefectural Gifu Hospital Noishiki, Gifu, Japan Megumi Watanabe, Ryo Matsumoto, Masayoshi Tsutsumi and Kazumasa Hikichi Special Reference Laboratories 51 Komiya-cho, Hachioji, Tokyo, Japan Key words : Chlamydia trachomatis SyphilisMicro Trak It was attempted to detect Chlamydia trachomatis from urethral smear of male and from cervical specimens of female by FITC-labeled monoclonal antibody (Micro TrakTM Direct Specimen Test). C. trachomatis was detected from 8 (33.3%) of 24 female with primary or secondary syphilis and from 1 (11.1%) of 9 male in the Dermatological Clinic of Gifu Prefectural Gifu Hospital from January 1985 to March Chlamydia-positive patients were treated with minocycline 100mg twice daily for seven days. Three weeks after the treatment, reexamination of the test for all the patients revealed free of C. trachomatis.
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