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さあしゃえいさか
5 years ago
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1 er. 3.0SJ

2 ver.3.0 SJ 3 2 1

3 ver.3.0 SJ () 400 2

4 ver.3.0 SJ OH OMe OMe O O O MeO O MeO OMe O O OMe O OMe OMe O O MeO O O H O O MeO OMe O H O OH OMe O CH 2 HO O O O O CH 3 OH HO OH OH HO OH O 1 3

5 ver.3.0 SJ 35 2 [2000 Osteoporosis Japan 9 (1) :9-14, 2001] 2 4

6 ver.3.0 SJ 2 5

7 ver.3.0 SJ - - SCF SCF 3 6

8 ver.3.0 SJ µg/ml /well) cont (µg/ml) (µg/ml) 4 ICR5 OVX Sham 7 AIN-93G 7

9 ver.3.0 SJ 1 OVX Sham 10 g/ OVX 3 1 Control 1 2) 50% 5 g// 2 1% 3 2% 50% 2 MX-1 3 MX-2 Sham 4 Sham AIN-96 1 Man S. L., Tamaki H., Ohta Y., Katsuyama N., Chinen I. Effect of exercise on osteopenia caused by restricted food intake in rats. J. Jpn. Soc. Nutr. Food Sci., 56, (2003). 2 Man S. L., Ohta Y., Katsuyama N., Tamaki H., Oku H., Chinen I. Effect of estrogen on osteoporosis caused by restricted food intake in rats. J. Jpn. Soc. Nutr. Food Sci., 55, (2002) ELISA (ng/ml) 4 (ng/ml) Control MX MX Sham MX-1 1 MX-2 2 Sham MeanSDn= pqct 2 8

10 ver.3.0 SJ 2 (mg/cm 3 ) (mg/cm 3 ) Control MX MX Sham MX-1 1 MX-2 2 Sham MeanSDn= (10-5 Nm) (mm) Control MX MX Sham MX-1 1 MeanSDn=4 MX-2 2 Sham 9

11 ver.3.0 SJ () (µg/ml) 5 B16 () (µg/ml) 6 B16 10

12 ver.3.0 SJ mJ/cm 2 3 UVB L * 7 800mg/kg L 0mg/kg mg/kg cont. 200mg/kg 400mg/kg 800mg/kg 11

13 ver.3.0 SJ RT-PCR tyrosinasetyrosinase related proteinmelanocortin receptor 1endotherin A receptor COX-2 mrna melanocortin receptor 1 endothelin A receptor COX-2 Control L Normal %L Normal 8 Normal Control 0.11%1% Normal Control 0.1% 1% 1%

14 ver.3.0 SJ 4 Conc. (%) L Normal ±0.52 Control ± ± ± ±1.11 (n=5) Control L Normal mg/kg L Normal 10 mg/kg 5 Dose(mg/kg) L Normal ±0.71 Control ± ± ± ±0.87 (n=5) Control tyrosinasetyrosinase related proteinendotherin A receptor mrna Normal 6 1% mrna COX-2 endotherin A receptor 0.1% 1% mrna 1% 6 mrna Normal Control 0.1% 1% 1% Tyrosinase 0.26± ± ± ± ±0.07 Tyrosinase related 0.28± ± ± ± ±0.10 protein Melanocortin receptor ± ± ± ± ±0.07 COX ± ± ± ± ±0.05 Endothelin A receptor 0.23± ± ± ± ±

15 ver.3.0 SJ Control Melanocortin receptor 1endotherin A receptor mrna Normal 7 mrna 7 mrna Normal Control (mg/kg) (mg/kg) Tyrosinase 0.92± ± ± ± ±0.07 Tyrosinase related 0.89± ± ± ± ±0.01 protein Melanocortin receptor ± ± ± ± ±0.02 COX ± ± ± ± ±0.02 Endothelin A receptor 0.92± ± ± ± ± () (µg/ml) 9 14

16 ver.3.0 SJ DPPH DPPH() (µg/ml) 10 DPPH SOD 15

17 ver.3.0 SJ SOD() BG99% 49.5%BG 80 1% 0.5% (µg/ml) 11 SOD 16

18 ver.3.0 SJ 12 17

19 ver.3.0 SJ () () ph3 ph7 ph9 0 ph3 ph7 ph9 14 ph ph

20 ver.3.0 SJ 100 g LD 50 30g 5 ICR 5000mg/kg LD mg/kg 19

21 ver.3.0 SJ -P -PC 5kg -L -LC 5kg -P -L -PC INCI Citrus Unshiu Peel Extract -LC BG INCI Butylene Glycol Citrus Unshiu Peel Extract 20

22 ver.3.0 SJ ml 96 plate l 375% CO g/ml01000g/ml 4 TRAP 13 ICR5 OVX Sham 7 AIN-93G1 OVX Sham 10 g/ OVX 3 1 Control 50%5 g// 2 1% 3 2% 50% 2 MX-1 3 MX-2 Sham 4 Sham AIN ELISA 4 ELISA Mouse TRAP AssaySuomen Bioanalytikka Oy SBA Sciences -80 pqct mM (ph:6.8)1360µl0.4mg/mll-dopaacros 500µL DMSO 40µL sigma 100µL(300units/mL) 5 490nm 6 B16 B M cells/ml 2ml 100g/ml 3 0.1Triton100 1N NaOH nm mJ/cm 2 3 UVB L 8 4, 5, 6, 7-0.5% 0.1 1% 21

23 ver.3.0 SJ Hos; HRM2, 5 12 UVB160 mj/cm ml UVB 320 mj/cm 2 16 L RNeasy TM Protect Mini Kit RNA RT-PCR tyrosinasetyrosinase related proteinmelanocortin receptor 1endotherin A receptor COX-2 mrna 9 NG1RGB cells/well 1FBS MEM MTT 10 DPPH 70% DPPH (1,1-diphenyl-2-picrylhydrazyl) DPPH SOD SOD 12 (1%)2 0.5%Control 99% 49.5% 13 BG 1 50µL Corneometer CM825 (Courage-Khazaka Electronics GmbH) 22

24 ver.3.0 SJ 23

25 ver.3.0 SJ 24

26 ver.3.0 SJ 25

27 ver.3.0 SJ 26

28 ver.3.0 SJ P

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untitled ORYZA CERAMIDE -PTPCDP8TP20CD -WSPWSP8 -LL0.8 -PCPC8PC20 -WSPCWSPC8 -LCLC0.8 Ver.13.0HS ORYZA CERAMIDE Ver.13.0HS 1. 2. 20 NMR 4 ( 1) 1 Ver.13.0HS HO CH 2 OH O O OH OH OH C CH CH H 2 NH C O R C H C H (CH