- PDF Free Download

Size: px

Start display at page:

Download ""

なおみわたぬき
7 years ago
Views:

3 gtf Wnt5a-Ror2 IL-8 in vitro

4 Ozony Tron P. gingivalis SOD ELISA Location of main occluding areas and masticatory ability in patients with reduced occlusal support

5 12-Methacryloyloxydodecylpyridinium bromide MDPB MDPB PB PB EPMA Streptococcus mutans PB WPB WPB WPB EPMA WPB PB FS WPB PB FS

6 Streptococcus mutans nm WPB MDPB MDPB WPB EPMA WPB Streptococcus mutans WPB

7 Poly P Poly P Ca Poly P Poly P MC3T3-E 1 ALP p-nitrophenyl phosphate substrate, ph10.5 Fluoresceinisothiocyanate FITC annexin V propidium iodide PI flow cytometory FCM total RNA reverse transcriptase-polymerase chain reaction RT-PCR Poly P polyethyleneimine thin layer chromatography PEI-TLC plate MC3T3-E1 Poly P Pi mmol/l MC3T3-E1 mmol/l Poly P ALP MC3T3-E1 mmol/l Poly P FCM Poly P

8 PI annexin V Poly P MC3T3-E1 osteocalcin β- Poly P MC3T3-E1 Poly P PEI-TLC Poly P Poly PPi PPi Pi3 Pi4 MC3T3-E1 β- Poly P Poly P Emdogain p Poly P Poly P Poly P Poly P Poly P Poly P Pi PPi Pi3 Poly P in vivo Poly P Poly P Poly P Poly P MC3T3-E1 in vivo in vitro

10 gtf CSI dft CSI dftcsi Real-time PCR S. mutans S.sobrinus gtf gtf gtf B gtf C gtf D

11 gtf I S. mutans gtf B D gtf B D S. sobrinus S. mutans gtf I gtf S. mutans S. sobrinus gtf B gtf C gtf D dft CSI dftcsi Real-time PCR S. mutans S. sobrinus S. mutans gtf B gtf D gtf

13 Wnt5a-Ror2 TNFα IL-1 Wnt5a Wnt5a Ror2 Wnt5a RT-PCR D Wnt5a GST-soluble Ror2 GST-sRor2 GST Ror2 Wnt5a Mφ M-CSF RANKL TRAP HE Ror2 Wnt5a GST-sRor2

14 GST Wnt5a Ror2 Mφ Wnt5a Mφ M-CSF RANKL Mφ Wnt5a Ror2 Wnt5a Wnt5a Wnt5a-Ror2 Wnt 5a Ror2 GST-sRor2 GST-sRor2 Wnt Wnt5a Ror2 Wnt5a Ror2 Wnt5a Ror2 RT-PCR Wnt5a Ror2 Ror Ror2 GST-sRor2Wnt5a Wnt5a Ror2 Wn5a-Ror2 Ror2 Wnt5a-Ror2 Wnt

15 Wnt5a Ror2 Ror2 Wnt5a Wnt5a-Ror2

16 IL-8 AZM in vitro AZM IL -8 IL-8 TNF-α AZM AZM in vitro MMP EM AZM JOM Porphyromonas gingivalis LPS ng/ml IL-6 IL-8 PGE ELISA µg/ml IL-6 IL-8 PGE MMP

17 AMZ LPS IL-8 EM JOM LPS IL-8 LPS IL-6 PGE MMP-2 MMP-9 AZM LPS IL-8 ERK JNK p38mapk PKA PI3K PLCγ NF-κB IL-8 NF-κB PGE AZM LPS IL-8 in vitro PGEIL-6 IL-8 AZM LPS IL-8 AZM IL-8 IL-8 IL-6 IL-8 PGE

18 1.8-cineol 1.8-cineol 1.8-cineol mg/ml Candida albicans JCM 1842 Klebsiella pneumoniae JCM 1662 Prevotella intermedia ATCC Prevotella nigrescens ATCC Actinobacillus actinomycetemcomitans JCM 8577 Porphyromonas gingivalis ATCC Streptococcus mutans Ingbritt Streptococcus sobrinus 6715 Streptococcus salivarius JCM cineol 1.8-cineol Streptococcus salivarius

19 1.8-cineol 1.8-cineol 1.8-cineol

21 µm

22 R SS SEP g SEP P N P -N µ P -N P -N

23 SEP SEP SEP

24 AIC χ Fisher s exact probability test

26 cdna PCR in situ ISH real time PCR myelin basic protein MBP MBP

27 ISH MBP MBP real time PCR myelin basic protein MBP PCR real time PCR in situ

28 in vitro PG EIL-6 IL-8 in vitro Porphyromonas gingivalis LPS ng/ml PGEIL-6 IL-8 ELISA MEK DMSO LPS COX -2 - mg/ml COX COX Inhibitor Screening Assay Kit COX Inhibitor Screening Assay Kit AcPLA ERK ERK LPS COX R http : //

29 LPS PGE LPS PGE LPS IL-6 IL-8 COX-1 COX-2 LPS COX-2 mg/ml LPS cpla ERK cpla MEK LPS PGE MEK LPS IL-6 IL-8 LPS PGE LPS PGE ERK ERK cpla PGE LPS in vitro PGEIL-6 IL-8 ERK cplapge in vitro

31 -L-

32 VF VE QOL VE VE VE VF

33 Wallenberg VF

34 BMP in vitro in vivo

36 R SS SEP A SEP P - N A A

37 P -N A P -N P -N P -N P -N A P -N P -N A A SEP A

38 A A

39 A Ca bfgf bfgf in vitro Gin-1-1 WST-1 Gin-1 / µm DMSO bfgf ELISA Gin-1 mm bfgf Student t p

40 Gin-1 bfgf in vitro in vitro Gin-1

41 Candida albicans C. albicans JCM1542 MCZ NCCLS MCZ MIC80 MCZ RPMI µg/ml MCZ µl GC mmφmm stroke / CFU/ml C. albicans µl SCDLP C. albicans µg/ml MCZ MCZ MIC -µg/ml RPMI µg/ml

42 MIC MCZ C. albicans µg/ml µg/ml - MCZ MCZ

43 Ozony Tron Ozony Tron Ozony Tron

44 Ozony Tron Ozony Tron

45 P. gingivalis SOD ELISA SOD Porphyromonas gingivalis P. g. SOD SOD Fe-SODMn -SOD P. g. SOD Amano, A J Bacteriol 172 : , 1990 P. gingivalis Fe-SOD Mn-SOD P. g. SOD P. g. SOD ELISA ELISA SOD SOD SOD microtiter plate ELISA P. gingivalis ATCC SOD ELISA SOD

46 pmolnmol P. g. SOD ELISA SOD SOD SOD SOD SOD ELISA SOD P. g. SOD ELISA P. g. SOD ELISA ELISA P. g. SOD pmolnmol P. g. SOD ELISA ELAISA P. gingivalis SOD SOD SOD ELISA SOD P. gingivalis P. g. SOD ELISA SOD P. gingivalis SOD P. gingivalis P. g. SOD ELISA SOD

47 Cambialistic SOD Fe Mn SOD P. g. SOD P. gingivalis P. gingivalis SOD

48 Location of main occluding areas and masticatory ability in patients with reduced occlusal support Eichner A1 Eichner B1 B4 C1 C3 mm PM M Eichner

49 Eichner A1 Eichner B C Eichner C1 Eichner Chi square test ; p Eichner A1 B1 C3 Eichner one-factor ANOVA ; p PM M Student t-test ; p Eichner