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7 10 DDS New York Academy Renal Biochemistry (Elsevier) Glutathione (John Wiley & Sons) Antioxidants in Therapy and Preventive Medicine (Plenum) The Liver: Biology and Pathophysiology (Raven Press) NO SSC

8 Cu Zn Se A C E 2

9 1) 1) 1) 2) 3) 4) 1) 1) 1) 2) 3) 4) 1) ( ) ( ) CR ( ) RE RVA X C A 1) 32 p Cu CuCl2 P 8 Cu CuCl2 1.0 mg% min Cu 30 min 1/2 Cu CuCl2 Cu Cu

10 AIN93G 20 g/g Wistar , 2) 3) 2) 1) 2) 3) F344/N 3 17 AIN-93G AIN-93G 7 Student t A/G GOT 2 GPT ALP -GTP T3 T4 p = 0.069

11 3 ICR 1 kg 2 mg AIN-93G 1 AIN93G 10 HPLC Ca 1) 1) 1) 1) 1) 2) 2) 1) 1) 2) -3 FP FP FPH CHOL CHOL BA in vitro ] <> FP FPH 5 Wistar AIN93 50 FP FPH CHOL0.5% 0.1% 6 4 CHOL BA <In vitro > FP FPH CHOL BA FP FPH CHOL CHOL CHOL CHOL BA In vitro FP FPH BA CHOL FP BA CHOL FPH FP BA FP FPH CHOL CHOL BA BA FP FPH

12 Ca Mg Ca Mg AIN-93G AIN-93G Ca Mg 3 SD Ca Mg Ca 9K(CBD9K) mrna Ca TRPV6 mrna Mg TRPM6 mrna Ca Ca Mg Mg Ca CBD9K mrna TRPV6 mrna TRPV6 mrna CBD9K mrna Mg Mg TRPM6 mrna TRPM6 mrna Ca Mg 1, 5) 2, 5) 3) 4) 1) ( ) 2) 3) 4) ( ) 5) 4, 5) TBT TBT Ca 2+ TBT TBT Ca RCR-1 TBT 1 μm caspase-3 Propidium Iodide Ca 2+ TBT Ca 2+ Dulbecco s PBS (DPBS) BAPTA-AM 2. TBT Ca 2+ Ca 2+ Fura2-AM Ca 2+ calpain 3. JC-1 cytochrome c TBT RCR-1 caspase-3 Ca 2+ Ca 2+ calpain Ca 2+ BAPTA-AM Ca 2+ DPBS calpain cytochrome c TBT RCR-1 Ca 2+ 2

13 40 50 QOL PVEE PVEE HPLC Slc:ddY 9 OVX PVEE 20 mg/kg 8 Deoxyprydinoline DPD Tartarate Resistant Acid Phosphatase TRAP TRAP X CT TRAP mrna RT-PCR PVEE OVX TRAPmRNA DPD TRAP PVEE PVEE TRAP PVEE OVX PVEE 8 OVX PVEE PVEE 1) 2) 2) 2) 1) 2) ) Ca K Cu Mn Se medium chain fatty acids: MCT) Fe Zn Ca Cu BALB/C C mL G-50 G-100 G ICP-OES Fe Ca G-50 G-100 G-150

14 1) 1) 2) 1) 2) mM NADH 2. NADPH NADPH mm mm 2 mm 4. in vitro GSSG NADPH Ki 5 10 mm NAD(P)H

15 - 1) 1) 2) 1, 2) ArgRPseudomonas taetrolens NBRC 3460 D- 1) MALDI-TOF MS MALDI-TOF MS ArgR 2 A DTT B A B ArgR MALDI-TOF MS C47A/C73A-ArgR C47A/C73A-ArgR ArgR C47A/C73A-ArgR ArgR C47A/C73A-ArgR MALDI-TOF MS ArgR Cys47 Cys73 PLP ArgR ArgR 2 Cys Ala C47A/C73A-ArgR ArgR DTT Cys47-Cys73 ArgR ArgR Cys47-Cys73 ArgR 1) D. Matsui, T. Oikawa et al., (2009) Appl. Microbiol. Biotechnol., 83: ) 1) 2) 2) 1) 1) 2) K K1 K2 K3 4 K 4 SXR 4 K K1 K K K1 4 7 K K3 K K3 K K 58 mol/d K1 4 7 K3 (n = 4 5) Sep-Pak Silica HPLC K K1 4 7 K K1 7 K3 4 K K 4 K3

16 - FAD FMN 2-2-CAA CAA 16S rrna 2-CAA 2-CAA 2-CAA Pseudomonas sp. YL 2-CAA ,301 CAA67_YL CAA67_YL FAD 2-CAA H218O [18O] 2-CAA FAD FADH2 CAA67_YL NAD(P)H AXXGXXG NADH NADPH FAD NADH FAD CAA67_YL NADH FAD FADH2 2-1) 1) 1) 2) 2) 1) ( ) 2) 20 ppm 1 11 Wistar 220 g mg /kg Wistar 180 g AUC AUC

17 1) 2) 1) 2) (EPO) Biol Trace Elem Res 107: , 2005 HO-1 Sprague-Dawley 3 AIN-93G CONZn 4.5 mg/kg diet 4.5 ppm ZD Pair-Fed AIN-93G PF HO-1 Ret Fisher PLSD 5 HO-1 CON ZD 3 Ret HO-1 CON ZD PF EPO EPO PF Wistar AIN93G mg/kg mg /ml 3.19 mg/ml 800 ml/kg mg/kg 12.5 mg/kg 12.5 mg/kg

18 1, 4) 2) 3) 3) 4) 1) ( ) 2) 3) ( ) 4) TBT TBT 1. Wistar g TBT 2.5 mg/kg body weight CYH CYH 0.1 ppm 1 ppm 10 ppm 100 ppm TBT CYH 2. TBT TBT GC/MS 3. Hematoxylin-Eosin HE TdT-mediated dutp-biotin Nick End Labeling (TUNEL) TBT CHY ppm 50%GC/MS TBT TBT 24 TBT TUNEL TBT 1) 2) 1) 1) 2) Rb Rb Rb Rb Rb Rb Rb Rb Rb 4 Wistar 62 8 Rb ,000 ppm 46 Rb Williams 5% Rb Rb Rb 1 ppm Rb Rb 8 25 ppm ppm ppm Rb ppm Rb μg/g/ppm , Rb Rb ppm Rb Rb ppm Rb ppm

19 % * 1) 2) 1) 1) 3) 1) 2) 3) μg μg/ g 216 g 44.8% 41.4% 37.9% 27.6% ± 113 μg ± 103 μg ± 120 μg ± 102 μg 90% 400 μg 30%

20 1) 1) 2) 1) 2) μg/ 2 μg/ 5 μg/ ± 4.0 FFQ L. plantarum ATCC N 4.5 N 100 μl 100 μl nm ng/ml 2.9 ng/ml 2.8 ng/ml 2.3 ng/ml 18.8 μg/mgcre 24.3 μg/mgcre 13.2 μg/mgcre 14.6 μg/mgcre p < μg 34.8 μg 35.2 μg 36.0 ± 7.2 μg/2010 1, 2) 1, 2) 2) 2) 1) 2) 75 10% 1) 60% 40% 2) (1) (1) Katayama M, Sugawa-Katayama,Y (2007) Effect of temperature on the diminution of retained arsenic in dried Hijiki, Sargassum fusiforme (harvey) Setchell, by water-soaking., J. Home Econ. Jpn, 58, (2) Katayama M, Sugawa-Katayama Y, Yamaguchi Y, Murakami K, Hirata S (2008) Effect of temperature on the extraction of various arsenic compounds from dried Hijiki, Sargussum fusiforme by water-soaking as a pre-cooking process. Trace Nutrients Research 25:

21 Se- GC-MS Se- - -Se- LC-MS g/g g 0.1 ml 0.45 m LC-MS Applied Biosystems, API 3000 Cadenza CD-C18 Imtact/70/ / ml/min Inertsil ODS-3 GL Sciences /80/ / ml/min - -Se- LC-MS LC Se- - -Se- Se- - -Se- LC-MS 1) 2) 3) 4) 1) 1) 1) 2) 3) 4) NaKCa Mg P S ZnFe Cu Ca Na Zn Fe 40

22 1) 1) 2) 2) 3) 3) 1) 1, 2) 1) 1) 2) 3) 1, 2) Klotho Klotho KO Klotho Klotho 500 g 550 g SD F 40 calorie restriction CR Klotho real-time PCR Klotho SDS-PAGE F ± 13.7 g ± 15.5 g F 60 CR ± 17.2 g ± 9.8 g Real-time PCR Klotho m-rna F CR 1.5 SDS-PAGE Klotho F CR Klotho

Microsoft Word - 14_LCMS_アクリルアミド

Microsoft Word - 14_LCMS_アクリルアミド 3--2-3-Iodo-2-propynyl butylcarbamate (IPBC) Carbamic acid, butyl-, 3-iodo-2-propynyl ester Iodocarb CAS 55406-53-6 C 8 H 12 NO 2 I C (g/cm 3 ) (mmhg) log P ow 281.09 (280.9910) 64 68 1.51 1.57 (20 C)