UPC 2 Baiba Cabovska, Michael D. Jones, and Andrew Aubin Waters Corporation, Milford, MA, USA UPC 2 ACQUITY UPC 2TM ACQUITY SQD Empower 3 UPC GC
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1 UPC 2 Baiba Cabovska, Michael D. Jones, and Andrew Aubin Waters Corporation, Milford, MA, USA UPC 2 ACQUITY UPC 2TM ACQUITY SQD Empower 3 UPC GC LC GC UltraPerformance Convergence Chromatography UPC 2 SFC HDPE LDPEEVA PVC 4 14 IPA IPA GC-MS IPA ACQUITY UPLC ACQUITY UPC 2 3 UPC 2 GC UPLC UPC 2 SFC UltraPerformance Convergence Chromatography TM 1
2 HDPE LDPEEVA PVC 2 g10 ml g 10 ml 20 ml GC-MS HP-5MS 30 m x 0.32 mm 1.0 µm 2 ml/ 1% B B 20% 30 1% B 65 APBR1800 psi 1.0 µl nm MS m/z kv 25 V MS 0.1% / 0.2 ml/ Empower µl ml/ 350 m/z MassLynx v4.1 UPLC A B ACQUITY UPLC ACQUITY UPLC BEH 2.1 x mm1.7 µm 0.1% / 0.1% / 0.9 ml/ 10 B 50% 90% 50% B UPC 2 ACQUITY UPC 2 PDA SQD ACQUITY UPC 2 BEH 2-EP 3.0 x mm1.7 µm A C2 B 1:1 / 2.0 ml/ 50 2 µl nm MS m/z V 3 kv Empower 2 UPC 2 2
3 LC GC GC LC UPC UPLC UPC 2 14 UPC 2 UPLC UPLC UPC 2 Irganox 1010 Irganox 245 GC-MS GC-MS Uvitex B GC LC UPC 2 UPLC UPC 2 IPA 2 3 LDPE UPLC IPA UPC 2 UPLC UPLC UPC 2 GC-MS GC-MS 4 UPC 2 3
4 H H P Cl-2-H-4- BP Cl H-2- BP S Uvitex B H H H Irganox 245 H H H H H H H H H Irganox 1330 Irganox 1010 H H Lowinox 44B25 H H Tinuvin 328 BHT augard 445 Tinuvin P 1. UPC 2 4
5 Irganox BHT 5-Cl-2-H-4- BP Tinuvin P Tinuvin H-4- BP Uvitex B augard 445 Irganox 1010 Irganox 245 Lowinox 44B25 ACQUITY UPC Date Acquired: 9/15/2012 7:48:39 AM EDT Sampleame: 14 PA mix Injection Id: Tinuvin P 5-Cl-2-H-4- BP BHT Irganox 245 Lowinox 44B25 2-H-4-4-H-2-octyloxy BP BP Uvitex B augard 445 Tinuvin 328 ACQUITY UPLC Irganox 1330 Irganox BHT 5-Cl-2-H-4- BP Tinuvin P Tinuvin H-4- BP augard 445 GC-MS Uvitex B 2. UPC 2 5
6 Column ame: 2-EP Sampleame: PET IPA mw Date Acquired: 9/6/2012 6:14:33 PM EDT Instrument Method Id: 1953 Injection Id: Column ame: 2-EP Sampleame: PET hex mw Date Acquired: 9/6/2012 4:10:57 PM EDT Instrument Method Id: 1953 Injection Id: 2014 PVC PVC Column ame: 2-EP Sampleame: EVA IPA mw Date Acquired: 9/6/2012 6:02:46 PM EDT Instrument Method Id: 1953 Injection Id: 2118 Column ame: 2-EP Sampleame: EVA hex mw Date Acquired: 9/6/2012 3:59:11 PM EDT Instrument Method Id: 1953 Injection Id: EVA EVA Column ame: 2-EP Sampleame: LDPE IPA mw Date Acquired: 9/6/2012 5:50:59 PM EDT Instrument Method Id: 1953 Injection Id: 2107 Column ame: 2-EP Sampleame: LDPE hex mw Date Acquired: 9/6/2012 3:47:25 PM EDT Instrument Method Id: 1953 Injection Id: LDPE 0.02 LDPE 0.00 Column ame: 2-EP Sampleame: HDPE IPA mw Date Acquired: 9/6/2012 5:39:12 PM EDT Instrument Method Id: 1953 Injection Id: 2096 Column ame: 2-EP Sampleame: HDPE hex mw Date Acquired: 9/6/2012 3:35:37 PM EDT Instrument Method Id: 1953 Injection Id: 1981 HDPE HDPE IPA IPA ACQUITY UPC 2 % % % % HDPE IPA microwave _018 Sm (Mn, 2x3) PVC _017 Sm (Mn, 2x3) EVA : TF MS AP+ 2.19e6 1: TF MS AP+ 2.19e : TF MS AP _016 Sm (Mn, 2x3) 2.19e6 LDPE _015 Sm (Mn, 2x3) 1: TF MS AP+ 2.19e6 HDPE % 0% 0% 0% HDPE hexane microwave _007 Sm (Mn, 2x3) PVC EVA LDPE HDPE : TF MS AP+ 2.14e _006 Sm (Mn, 2x3) : TF MS AP+ 2.14e _005 Sm (Mn, 2x3) : TF MS AP+ 2.14e _004 Sm (Mn, 2x3) : TF MS AP+ 2.14e IPA 4. IPA GC-MS ACQUITY UPC 2 5 Irganox LDPE MS 6 7 LDPE IPA Irganox Minutes 5. ACQUITY UPC 2 LDPE UPC 2 6
7 2.0x10 7 Combined - SQ 1: MS Scan 1: ES+, Centroid, CV=Tune x x x x x x x10 6 Intensity 1.6x x x x x x x x m/z 5.0x UPC 2 LDPE Irganox x10 7 Combined - SQ 1: MS Scan 1: ES+, Centroid, CV=Tune x x x x x x x10 6 Intensity 1.4x x x x x x x m/z 2.0x UPC 2 GC-MS UPC 2 7
8 ACQUITY UPC 2 LC GC UPC 2 GC UPC 2 UPLC 1/2GC 1/8 1. Balogh MP. Testing the Critical Interface: Leachables and Extractables. LCGC June. 2. Containers Closure Systems for Packaging Human Drugs and Biologics. Guidance for Industry; U.S. Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER) and Center for Biologics Evaluation and Research (CBER) May. 3. orwood DL, Fenge Q. Strategies for the analysis of pharmaceutical excipients and their trace level impurities. Am Pharm Rev. 2004; 7 (5) : 92,94, MS WatersACQUITYACQUITY UPLCUPC 2 EmpowerMassLynx The Science of Whats Possible Waters Corporation ACQUITY UPC 2 UltraPerformance Convergence Chromatography Waters Corporation 2013 Waters Corporation. Printed in Japan JAPDF
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UPC 2 Andrew Aubin Waters Corporation, Milford, MA, USA Waters ACQUITY UPC 2TM 6 tert- UltraPerformance Convergence Chromatography TM UPC 2 ACQUITY UPC 2 1 A A D3 E D3 K1 K2 UPC 2 A E K1 ACQUITY UPC 2
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Ultra Performance Liquid Chromatography Stephan Koza, Paula Hong, and Kenneth J. Fountain Waters Corporation, 34 Maple Street, Milford, MA, USA & SEC 2010 2 8 5 2030 4 3 8 1982 2009 131 USP EPHMW USP34
Waters Sigma-Aldrich 1.0 mg/ml LC Waters Alliance HPLC ACQUITY UPLC H-Class Bio 30 cm Alliance HPLC UV ACQUITY UPLC TUV mm nm XBridge Protein B
HPLC 3. µm BEH Stephan Koza, Susan Serpa, Hua Yang, Edouard Bouvier, and Kenneth J. Fountain Waters Corporation, Milford, MA, USA HPLC 200 0 HPLC 2 2010 UPLC 200 SEC 1 UPLC SE-UPLC 2 µm BEH BEH 100% SE-UPLC
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1 WSV SIR m/z Analyte RT (Min) SIR m/z Cone voltage (V) Ascorbic Acid (C).91 177 2 Thiamine (B1) 1.1 265 5 Nicotinic Acid (B3) 1.27 124 15 Pyridoxal (
ACQUITY UPLC H-Class ACQUITY QDa Mark E. Benvenuti, Dimple Shah, and Jennifer A. Burgess Waters Corporation, Milford, MA, USA MS ACQUITY QDa UPLC HPLC UV ACQUITY QDa LC EC 1925/26 CFR 21 HPLC 1 LC-MS ACQUITY
Sigma-Aldrich (Poole,Dorset,UK) LGC Standards(Teddington,Surrey,UK) 1 mg/ml Sigma-Aldrich Greyhound Chromatography(Brikenhead,UK) / Waters ACQUITY UPL
ACQUITY UPLC I-Class/Xevo TQD Mark Roberts and Michelle Wood Waters Corporation, MS Technologies Centre, Manchester, UK ACQUITY UPLC I-Class/Xevo TQD 2 2 MRM 178 / 950 MS ACQUITY UPLC/ACQUITY TQD 2 1-3
HILIC UPLC/MS UPLC LC/ MS LC/MS HPLC 2-4 HPLC 4,5 HILIC / HILIC 80 ACQUITY UPLC Xevo QTof MS ACQUITY BEH HILIC HILIC TOF ESI 1.7 µm BEH HILIC UPLC HIL
HILIC UPLC/MS UPLC LC/ MS LC/MS HPLC 2-4 HPLC 4,5 HILIC / HILIC 80 ACQUITY UPLC Xevo QTof MS ACQUITY BEH HILIC HILIC TOF ESI 1.7 µm BEH HILIC UPLC HILIC UPLC HPLC 1 100 µl 900 µl 15 900 µl 500 µl 470 µl
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UPLC-Tof-MS E John Archer, 1 Paul Dargan, 1 David Wood, 1 Nayan Mistry, 2 and Michelle Wood 2 1 Guy s and St. Thomas NHS Foundation Trust, Clinical Toxicology, London, UK 2 Waters Corporation, MS Technologies
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固相抽出 ビギナー ズ ガイ ド 日本ウォーターズ株式会社 日本ウォーターズ株式会社 東京本社 東京都品川区北品川 第 5 小池ビル TEL FAX 大阪支社 大阪市淀
固相抽出 ビギナー ズ ガイ ド 日本ウォーターズ株式会社 日本ウォーターズ株式会社 www.waters.com 東京本社 140-0001 東京都品川区北品川 1-3-12 第 5 小池ビル TEL 03-3471-7191 FAX 03-3471-7118 大阪支社 532-0011 大阪市淀川区西中島 5-14-10 サムティ新大阪フロントビル 11F TEL 06-6304-8888 FAX
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