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2 1 Phaeocystis sp Phaeocystis sp ATP 4-3
3 21 (1,500 ha/year) Phaeocystis sp Aspergillus Streptomyces ATP Phaeocystis sp. Phaeocystis sp.7.5% % 2 ATP Streptomyces Phaeocystis sp. ATP Aspergillus Phaeocystis sp. 0% Streptomyces Aspergillus ( 1
4 ) 2
5 ( 51) 616 ( 2.3%) ( ) 21 NGO 1,500 ha Phaeocystis sp. (1 ) (1.) 3
6 ( ) 8 ( ) Phaeocystis sp. ( ) 2 (Phaeocystis sp.) 1. 4
7 1 Phaeocystis sp. Phaeocystis sp. Phaeocystis sp. Haptose Haptose Haptose Haptose Phaeocystis sp. 1.HaptosePhaeocystis sp. Phaeocystis sp. ( ) Phaeocystis sp. (PES) Phaeocystis sp 1PES 16 ( 3,000Lux) ml 100l (20l/min) 4 2. Phaeocystis sp.100l 4 Phaeocystis sp. 5l Phaeocystis sp. (4,000g, 20min) Phaeocystis sp. 5
8 Phaeocystis sp. Phaeocystis sp. Phaeocystis sp. 3 3 Phaeocystis sp. Phaeocystis sp. Phaeocystis sp. 5 5ml Phaeocystis sp. (100,5min) 200 l 5% 200l 1ml 20 (SHIMADZU) (490nm) Phaeocystis sp. 1ml 9ml 6% 1.5MNaOH 1.5ml (121,30min) 3.5M 0.5ml (220nm) (6.25) Phaeocystis sp. 1:1 ()24 (90cm) 6
9 Phaeocystis sp. (105) (600w) Phaeocystis sp Phaeocystis sp. 1. Phaeocystis sp. 7
10 (2.( - )) 8 ( 3.) 8 () 8
11 ( )Phaeocystis sp.9:1 2.5%(w/w) (2222[mm]) (50,24hr ) ( 4.) ml 100l 1ml ( CMC ) 7 ( CMC ) CMC 10ml/
12 CMC (OLYMPUS,BX-50) (FUJIFILM,HC-300Z) ( ( )) Hutter ( )) ATP ( ) ( ) 2 2 CMC 10
13 1 (5ml) mg/ml 0.1%Tween l l l l l 5. (15ml) 202mg CMC 2ml (121,20min) (Wako,Trichoderma viride,990[unit/g])0.1%tween ,1,2,10,20[mg/ml] 100l 30,120rpm (7,000g,5min) 1.5ml 11
14 (12,000rpm(TOMY:MRX-150),5min) 592nm (No1, 55mm,ADVANTEC) (15ml)202mg CMC 2ml (121,20min) 100l (30,120rpm) (121,20min) (7,000g,5min) 1.5ml (1,2000rpm(TOMY:MRX-150),5min) 592nm (SHIMADZU) ATP ATP CMC 1.9ml (121,20min) 100l (30,120rpm) 4 ATP (7,000g,5min)200l (, TES-Buffer(TES,MgSO4,pH7.5)6 )) (LUMICOUNTER,ADVANTEC) 5 TEA-NRB 1% 100 l (Sonicator,Heatsystem-ultrasonic) ( Lv.6,5s) 1.9ml TES-Buffer 12
15 Dimethyl Sulfoxide (DMSO) 200l DMSO l Na3PO4 (0.01M) 1 (Yamato,Bransonic)2 1% 100l 900l TES-Buffer TES-Buffer 2ml 5 Sonic TES-Buffer 2ml (Lv.6,5s) ATP ATP ATP 1 20ml CMC (50ml )1 20ml 200ml CMC (500ml ) 1 ( ADVANTEC,No.101,55mm) ATP ATP (15ml)201mg 1.9ml 13
16 100l (120rpm,30) ATP (Sonicator,Lv.6,5s) (7,000g,5min) 200 l (, )
17 (3.) (4.) 4. (C )
18 2-5-2 ( omycetaceae ( ( 20 ) (1m ) ( 7.) 7.) Strept 8.) ( 9.) Streptomyces ( Str.) Streptomyces 16
19 (1m ) Aspergillus ( Asp. ) 10. (12.) ( 11.) 1 ( 11.) ( 11.) ( 10.)Aspergillus. niger Aspergillus. awamori 11. Aspergillus
20 Asp. (50,1 ) Str.24 (50) Str (:Str. :Asp.) 14. (:Str. :Asp.) (4.) 4. 18
21 0mg (5.) 5. 20mg 9 Str. 8mgAsp. 10mg ( ) ( ) 19
22 ATP (6.) 6.ATP DMSO Aspergillus TEA-NRB Streptomyces Sonic 20
23 ATP (7.,8.) 7.Str. ATP 8.Asp. ATP ATP Str. Asp. ATP (Str.:Asp.=5:1) Str. Asp. ATP Str.. Asp. ATP [pmol/mg] [g/mg] [ g/mg] ATP Str. 3036%Asp. 4353% Str %Asp % Str %, Asp % 21
24 ATP (9.) ATP (7.,6.) 9. ATP ( ) Str.1mg Asp.0.6mg ( ) ATP ( ) 22
25 3 Phaeocystis sp Phaeocystis 2.5g/ 100ml/ 50 (E Phaeocystis sp. sp. 0:100 20: (10.) 0.25kg (24 ) mm (50mm/min) [mm] 3mm (50mm/min) [mm] 100[N] 100[N] 3mm Ztest,SHIMADZU) 50mm/min 100N (11.)
26 [mm] 10mm
27 N Ph 12% Phaeocysti sp. 12 Phaeocystis sp. 0% 12% (12.) 12.Ph 100N Phaeocystis sp. 7.5% 2.5Kg 25
28 3-4-3 ( ) ( ) 7.5% (1 3.) ( ) Phaeocystis sp. Phaeocystis sp.7.5% 26
29 4 4-1 Phaeocystis sp. 7.5% ATP Phaeocystis sp mg 0:100 12:88 ( 5555[mm]) (TOYOSEKI) 11015Mpa (15ml) (121,20min) 0.9m (1mg/ml,0.1%Tween80)0.1ml 30,120rpm 4 ( )2 27
30 4-2-3 ATP 1ml Buffer (Lv.6,5s) ATP (7,000g)200 l ATP ( ) 24.5ml TES 500l 4-3 Str. 0 7 ATP 14. Phaeocystis sp. Str.ATP Phaeocystis sp. Str.Phaeocystis sp. Asp. 28
31 Asp. 0 7 ATP (15.) 15. Phaeocystis sp. Asp.ATP ATP (16.) ATP 16. Phaeocystis sp Asp.ATP 29
32 Asp. Asp. Asp.Phaeocystis sp. 99 Asp. Phaeocystis sp.12 Phaeocystis sp. 30
33 2 Ph 7.5% Ph ( ) 100[kgf/cm2] Phaeocystis sp. Phaeocystis sp. 31
34 Str. Asp.Str. 2 2 Str. Phaeocystis sp. Asp. 2 ( ) 32
35 3 33
36 (1978) - - (1992) (1992) (1998) (1996) () (1995) () (1978) () (1978) () (1992) Raymond P. Poincelot and Peter R. Day(1972) Simple dye Release Assay for Determining Cellulolytic Activity of Fungi. Applied Microbiology,23(5), J.G.Savory,B.Mather,C.C.Maitland and K.Selby(1967) Methods,Apparatus:New Product Reserch,Process Development and Design. Chemystry and Industry, January(28), R.E.Smith(1977)Rapid Tube Test for Detecting Fungal Cellulase Production. Appled and Environmental Microbiology,33(4), Stanisla Leznicka(1980)Cellulolytic Activites of Fungi Destroying Ancient Books. Acta Microbiologica Polonica,29(4), W. Verstraete, H. Van de Werf, F. Kucnerowicz, M. IlaiwiL. M. J. Verstraeten and K. Vlassak(1983) Specific measurement of soil microbial ATP. Soil Biology and 34
37 Biochemistry, 15(4), K. Inubushi, P. C. Brookes and D. S. Jenkinson(1989)Influence of paraquat on the extraction of adenosine triphosphate from soil by trichloracetic acid, Soil Biology and Biochemistry,21(5), J. M. Oades and D. S. Jenkinson(1979)Adenosine triphosphate content of the soil microbial biomass, Soil Biology and Biochemistry,11(2), F. Eiland(1983)A simple method for quantitative determination of ATP in soil, Soil Biology and Biochemistry,15(6), Q. Y. Bai, L. Z elles, I. Scheunert and F. Korte(1988)A simple effective procedure for the determination of adenosine triphosphate in soils,chemosphere,17(12), D. S. Jenkinson and J. M. Oades (1979) A method for measuring adenosine triphosphate in soil, Soil Biology and Biochemistry,11(2),
HPLC 1M KH 2 PO 4 ph ml Anthranilic acid: AnA 600 ml ml AnA 1.0 ml / min Anthranilic acid= PRESSURE 140 kgf / cm 2
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94.7 05.1 1 mm 2 3 1) 2) 3) 4 3- -2-3- -2-..... mm 5 ,,,, (15,000ppm) (15,000ppm) (1,000ppm) (5,000ppm) American Conference of Governmental Industrial Hygienists 3,000 6,000 12,000 18,000 6 g/ml Aspergillus
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- 1 - HIV cmc cmc - 2 - cmc() ( ) 5-3 - - 4 - (1) (2) (3) ( ) - 5 - 0537-73-2662 0537-35-2971 0548-63-1251 9 00 16 00-6 - - 7 - 2 3 4 5 6 1,328 1,848 2,368 2,888 3,408-8 - - 9 - - 10 - - 11 - 3-12 - (
3 光毒性第三者評価報告書100924改
21 5 13 22 5 17 1 2 in vivo 24 in vivo 9 6 6 GLP 1 b 4 Acridine BMDM 4-t-butyl-4-methoxydibenzoylmethane CHD Chlorhexidine Bithionol CHD Bithionol in vivo in vivo b in vivo 70% b 5 in vitro 3T3-NRU 4 9
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- 110 - - 111 - - 112 - - 113 - - 114 - - 115 - - 116 - - 117 - - 118 - - 119 - - 120 - - 121 - - 122 - - 123 - - 124 - - 125 - - 126 - - 127 - - 128 - - 129 - - 130 - - 131 - - 132 - - 133 - - 134 - -
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300 10 16 1695 4 2 44 7 3 1 2 2 1869 11 192210 506.75ha 3 (1594) (1596) 16 17 7 1630 2 14 1637 16 17 4 1664 5 6 1678 6 1678 6 3 8 16954 8 (1758) 7 (1770) 17 2 (1745) 2 (1765) 4 11 11 10 12 1 12 12 12 2
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1,985ha 41.5 2,799ha 58.5 4,784ha 100.0 3 14,210 0 0.0 27 57,590 37,140 64.5 3 1,800 930 51.7 6 1,150 1,150 31.0 39 77,310 39,220 50.7 34 8-3 34 8-4 52 1-6 52 2-3 53 1-6 56 1-2 56 1 18 12 17 30 50