Appilation Note
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- よしお ほうねん
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1 LAAN-C-XX011A Lifescience
2 O HO O MS 1 MS 2 C 14 H 16 N 10 HO HO O MS 3
3 Application Note No.10
4
5 Application Note No.10
6
7 Application Note No.10 Intensity (x100,000,000) :TIC -:TIC +: (20) +: (2.00) +: (2.00) +: (2.00) +: (2.00) +: (2.00) -: (2.00) -: (2.00) -: (2.00) -: (2.00) min Peaks 1: (-)-Gallocatechin, 2: Theophylline, 3: (+)-Catechin, 4: (-)-Epigallocatechin, 5: Caffeine, 6: (-)-Epigallocatechin gallate, 7: (-)-Epicatechin, 8: (-)-Gallocatechin gallate,9: (-)-Epicatechin gallate, 10: (-)-Catechin gallate Intensity (x100,000,000) A B C D E %RSD Area R.T. A B C D E min
8 ( 10,000,000) TIC ID Compound Name RT ID Compound Name RT 1 L-Alanine-2TMS Lyxose methyloxime-tms Oxalic acid-2tms Asaparagine-3TMS Malonic acid-2tms Lyxose methyloxime-tms Urea-2TMS 6.27 Ribitol-5TMS (IS) Serine-2TMS L-Glutamine-4TMS Methyl 5-oxo-2-pyrrolidinecarboxylate Xylonic acid-3tms Ethanolamine-3TMS Theanine L-Leucine-2TMS L-Glutamine-3TMS Phosphoric acid-3tms Shikimic acid-4tms L-Isoluecine-2TMS Citric acid-4tms L-Threonine-2TMS Caffeine L-Proline-2TMS Quinic Acid- TMS Glycine-3TMS Fructose-Methyloxime-5TMS Succinic acid-2tms Fructose-Methyloxime-5TMS Glyceric acid-3tms Glucose-Methyloxime-5TMS Fumaric acid-2tms L-Lysine-4TMS L-Serine-3TMS Mannose-Methyloxime-5TMS ,4-Bis[(trimethylsilyl)oxy]dihydro-2(3H)-furanone-2TMS L-Tyrosine-3TMS L-threonine-3TMS Benzoic acid-4tms L-Aspartic acid-2tms Gluconic acid-5tms Ketoglucose methyloxime-3tms Palmitic acid-tms Methylmaric acid-3tms Gluconic acid-5tms Arabino-Hexos-2-ulose-4TMS Inositol-6TMS Malic acid-3tms Galactose-Methyloxime-5TMS L-Aspartic acid-3tms Phosphoric acid propylester-4tms Pyroglutamic acid-2tms Stearic acid-tms aminobutyric acid-3tms L-Tryptophan-3TMS L-Norvaline-3TMS Xylopyranose-4TMS L-Threonic acid-4tms Glucose-6-phosphate-Methyloxime-5TMS Isopropylmalic acid-3tms Glucose-6-phosphate-Methyloxime-5TMS Glycerol-3TMS Glucoheptulose-Methyloxime-TMS Ornithine-3TMS Di-n-octyl phthalate L-Glutamic acid-3tms Maltose-8TMS Phenylalanine-2TMS Sucrose-8TMS Asparagine-4TMS Arabinopyranose-4TMS ,3,4,5-Tetrahydroxypentanoic acid-1,4-lactone-3tms Raffinose-8TMS 19.92
9 Application Note No.10 a) PCA Score plot 10 Low Rank 30 High Rank R 2 X(PC1) : 0.37 R 2 X(PC2) : 0.16 Q 2 : b) Loading plot of PC1 Peak X (Unknown) 0.15 High Rank p[1] Low Rank Apparatus GC/MS LC/MS Analyte type organic acids amino acids saccharides sugar alcohol catechines xantines
10 Inten.( 1,000,000) MS m/z Inten.( 1,000,000) MS m/z Inten.( 100,000) MS m/z Predicted formula of Peak1C 14 H 16 O 10
11 Application Note No.10 Theogallin C 14 H 16 O 10 Exact Mass : HO O O HO O HO m/z 153 C 7 H 5 O 4 + m/z 309 C 14 H 12 O 8 + m/z 327 C 14 H 14 O m/z calc mda m/z calc mda m/z calc mda m/z Inten.(x1,000,000) m/z calc mda m/z Inten.(x1,000,000) Inten.(x1,000,000) m/z calc mda 0.5 m/z calc mda m/z MS 1 MS 2 MS 3
12 A-IK
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