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1 東京大学大学院理学系研究科附属臨海実験所年報平成14年度 2002年４月 2003年３月 MMBS 1886

2 MMBS (Misaki Marine Biological Station) 2

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4 1. Amano, M., Y. Oka, T. Yamanome, K. Okuzawa and K. Yamamori: Three GnRH systems in the brain and pituitary of a pleuronectiform fish, barfin flounder, Verasper moseri. Cell and Tissue Res., 309: , Amano, M., Y. Oka, T. Yamanome, A. Takahashi and K. Yamamori: Immunocytochemical localization and ontogenic development of melanin-concentrating hormone in the brain of a pleuronectiform fish, the barfin flounder. Cell and Tissue Res., 311: 71-77, Deguchi, R. and M. Morisawa: External Ca 2+ is predomiantly used for cytoplasmic and nuclear Ca 2+ increases in fertilized oocytes of the marine bivalve Mactra chinensis. J. Cell Sci., 116: , Togo, T., J. M. Alderton and R. A. Steinhardt: Long-term potentiation of exocytosis and cell membrane repair in fibroblasts. Mol. Biol. Cell., 14: , Wirsig-Wiechmann, C. R. and Y. Oka: The terminal nerve ganglion cells project to the olfactory mucosa in the dwarf gourami brain. Neurosci. Res., 44: , Yoshida, M., M. Ishikawa, H. Izumi, R. De Santis and M. Morisawa: Store-operated calcium channel regulates chemotactic behavior of ascidian sperm. Proc. Natl. Acad. Sci. USA, 100: , Yoshida, M., M. Murata, K. Inaba and M. Morisawa: A chemoattractant for ascidian spermatozoa is a sulfated steroid. Proc. Natl. Acad. Sci. USA, 99: , Oka, Y.: Physiology and release activity of GnRH neurons. In Progress in Brain Research, Vol. 141 (ed. by I. S. Parhar), Elsevier Science B. V., , Oka, Y.:., 19: , :.,, , :., NHK, 62-81, :., NHK, , : 3 ()., Proceedings 1. Amano, M., A. Takahashi, T. Yamanome, Y. Oka, K. Okuzawa, K. Okubo, K. Aida and K. Yamamori: Three GnRH systems in the brain of barfin flounder. Zool. Sci., 19: 1487,

Yamamori: Immunocytochemical localization and ontogenic development of melanin-concentrating hormone in the brain of a pleuronectiform fish, the barfin flounder. Cell and Tissue Res.

5 2. Ishizaki, M., M. Iigo, N. Yamamoto and Y. Oka: Different modes of GnRH release from multiple GnRH systems of teleost brain-pituitary slices. Zool. Sci., 19: 1470, Itho, A., M. Fujinoki, T. Kawamura, K. Inaba and M. Morisawa: Analysis of 15KDa protein from flagella of salmonid fishes. Zool. Sci., 19: 1433, Kimura, Y., M. Yoshida and M. Morisawa: Cloning of _-adrenergic receptor gene regulating metamorphosis of the ascidian, Ciona savignyi. Zool. Sci., 19: 1456, Kiya, T. and Y. Oka: Identification of a novel type of glutamate receptor in the terminal nerve (TN)- GnRH neurons. Zool. Sci., 19: 1470, Krasznai, Z., M. Morisawa, S. Morisawa, Z. Krasznai, T. Tron and T. Marian: Role of ion channel and membrane potential in the initiation of carp sperm motility. Proceedings of the International Scientific Conference about Current Problems of Animal Genetics and Their Practical Application, , Oka, Y.: Control of behavioral motivation and the peptidergic neuromodulatory system. Neurosci. Res., Suppl., 26: S9, Oka, Y., N. Yamamoto and U. Takashima: Quantitative analysis of behavioral motivation and neuromodulatory GnRH peptidergic system. Zool. Sci., 19: 1470, Steinhardt, R. A., T. Togo and J. M. Alderton: Neurotoxins in studies of cell membrane repair and channel activation N-S Arch. Pharmacol., 365, R44-R44 Suppl. 2, Tanaka, H., K. Kubokawa, T. Mizuta, S. Nakayama and M. Morisawa: CELLFACT: sperm motility initiating substance released from the sperm of amphioxus. Zool. Sci., 19: 1433, Togo, T. and M. Morisawa: GPI-anchored aminopeptidase is involved in the acrosome reaction of sperm in the mussel Mytilus edulis. Zool. Sci., 19: 1443, Togo, T., J. M. Alderton and R. A. Steinhardt: Long-term potentiation of exocytosis and cell membrane resealing in fibroblasts. Mol. Biol. Cell, 13: 506a, Togo, T., J. M. Alderton and R. A. Steinhardt: Potentiation of exocytosis and cell membrane repair in fibroblasts. Cell Struct. Func., 27: 272, Wirsig-Wiechmann, C. R. and Y. Oka: The terminal nerve GnRH/FMRFamide neurons project to the olfactory mucosa in the dwarf gourami. Zool. Sci., 19: 1470, Yamamoto, N., H. Ito and Y. Oka: Glutamate may be a co-transmitter of GnRH-peptidergic neurons of the terminal-nerve. Zool. Sci., 19: 1470, Yoshida, M., H. Izumi, R. De Santis and M. Morisawa: Capacitive calcium entry controls sperm chemotaxis in the ascidian, Ciona intestinalis. Zool. Sci., 19: 1442, Yoshida, M., M. Murata and M. Morisawa: A sulfate steroid regulates ascidian sperm chemotaxis. Proceedings of 9th International Symposium on Spermatology, ,

Morisawa: Cloning of _-adrenergic receptor gene regulating metamorphosis of the ascidian, Ciona savignyi. Zool. Sci., 19: 1456, 2002. 5. Kiya, T. and Y.

6 1. Krasznai, Z., M. Morisawa, S. Morisawa, Z. Krasznai, T. Tron and T. Marian: Role of ion channel and membrane potential in the initiation of carp sperm motility. XX Genetic Days, Brno, Czech Republic, Togo, T., J. M. Alderton and R. A. Steinhardt: Long-term potentiation of exocytosis and cell membrane resealing in fibroblasts. 42nd Annual Meeting of the American Society for Cell Biology, San Francisco, Y. Oka: Terminal-nerve (TN) GnRH system; a peptidergic neuromodulatory system. Department of Biophysics and Cell Biology, University of Debrecen, Debrecen, Y. Oka: Neurobiology of peptidergic neurons using a fish brain as a model system. Department of Cell Biology, University of Oklahoma Health Science Center, Oklahoma, Y. Oka: Neurobiology of peptidergic neurons using a fish brain as a model system. Program in Human Biology, Neuroscience Program/Psychology Department, Stanford University, Stanford, Yoshida, M., M. Murata and M. Morisawa: A novel sulfated steroid released from the egg regulates store operated calcium channel to cause chemotaxis of ascidian spermatozoa. 9th International Symposium on Spermatology, Cape Town, South Africa, : 3 GnRH 73, : GnRH GnRH 73, : 15kDa. 73,, : camp. 75,, : cdna PKA.,, :. 6,, : _. 73,, : _., : GnRH 73, :.,, : : 4

42nd Annual Meeting of the American Society for Cell Biology, San Francisco, 2002. 3. Y. Oka: Terminal-nerve (TN) GnRH system; a peptidergic neuromodulatory system.

7 ., : (SAAF).,, : GnRH GnRH, 73, Oka, Y: Control of behavioral motivation and the peptidergic neuromodulatory system.,, : GnRH 73, : CELLFACT:. 73,, :.,, : GPI. 73,, , J. M. Alderton and R. A. Steinhardt:. 55,, Wirsig-Wiechmann, C. R. and Y. Oka: The terminal nerve GnRH/FMRFamide neurons project to the olfactory mucosa in the dwarf gourami. 73, : GnRH co-transmitter 73,, R. De Santis: store-operated Ca 2+ channel (SOC). 73,,

73,, 2002. 17. :.,, 2002. 18. : GPI. 73,, 2002. 19., J. M. Alderton and R. A. Steinhardt:. 55,, 2002. 20. Wirsig-Wiechmann, C. R. and Y.

8 Arnaud, Zobel 9. Boo, Sung Min Chungnam National University 10. Cho, Ga Youn Chungnam National University

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17 220. Fernald, Russel Stanford University (U.S.A.) Social regulation of the brain Huitorel, Philippe URA CNRS, Marine Station Villefranche sur Mer, France 222. Krasznai, Zoltan University of Medical School of Debrecen (Hungary) 223. Marian, Terez University of Medical School of Debrecen (Hungary) 224. Trowbridge, Cynthia Oregon State University (U.S.A.) D Williamson, Roddy University of Plymouth (U.K.) 226. Wirsing, Celeste University of Oklahoma Health Science Center (U.S.A.) Regulation of sperm motility Reproduction biology; electrophysiology Reproduction biology; nuclear medicine Marine community and population ecology; feeding ecology of ascoglossan (=sacoglossan) opisthobranch molluscs; herbivore-algal interactions Neurobiology A comparative and multidisciplinary approach to studying the function

225. Williamson, Roddy University of Plymouth (U.K.) 226. Wirsing, Celeste University of Oklahoma Health Science Center (U.S.A.

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20 CONTRIBUTIONS FROM THE MISAKI MARINE BIOLOGICAL STATION UNIVERSITY OF TOKYO Nos Akanuma, T., S. Hori, S. Darras and H. Nishida: Notch signaling is involved in nervous system formation in ascidian embryos. Dev. Genes Evol., 212: , Amano, M., Y. Oka, T. Yamanome, K. Okuzawa and K. Yamamori: Three GnRH systems in the brain and pituitary of a pleuronectiform fish, barfin flounder, Verasper moseri. Cell and Tissue Res., 309: , Amano, M., Y. Oka, T. Yamanome, A. Takahashi and K. Yamamori: Immunocytochemical localization and ontogenic development of melanin-concentrating hormone in the brain of a pleuronectiform fish, the barfin flounder. Cell and Tissue Res., 311: 71-77, Deguchi, R. and M. Morisawa: External Ca 2+ is predomiantly used for cytoplasmic and nuclear Ca 2+ increases in fertilized oocytes of the marine bivalve Mactra chinensis. J. Cell Sci., 116: , Kato, T. and F. Pleijel: A revision of Notophyllum Örsted, 1843 (Phyllodocidae, Polychaete). J. Nat. Hist., 36: , Kawamura, M., M. Matsumoto and M. Hoshi: Characterization of the sperm receptor for acrosome reactioninducing substance of the starfish, Asterias amurensis. Zool. Sci., 19: , Kitazato, H., H. Nomaki, P. Heinz and T. Nakatsuka: The role of benthic foraminifera in deep-sea food webs at the sediment-water interface: Results from in situ feeding experiments in Sagami Bay. Frontier Res. on Earth Evol., 1: , Kitazato, H., M. Tazume and M. Tsuchiya: Phylogenetic relationships among the species of genus Bulimina (Foraminifera) inferred from ribosomal DNA sequences. Frontier Res. Earth Evol., 1: , Komai, T. and M. Takeda: A new deep-water shrimp of the genus Pandalopsis (Decapoda, Caridea, Pandalidae) from Sagami Bay, Japan. Bull. Natn. Sci. Mus., Ser. A. (Zool.), 28: , Koyanagi, M., A. Terakita, K. Kubokawa and Y. Shichida: Amphioxus homologs of Go-coupled rhodopsin and peropsin having 11-cis- and all-trans-retinals as their chromophores. FEBS Letters, 531: , Matsumoto, M., O. Kawase and M. Hoshi: Asterosap-induced calcium entry mechanism is much more sensitive to cgmp than to camp. Proceedings of 9th International Symposium on Spermatology, 77-80, Miya, T. and H. Nishida: Expression pattern and transcriptional control of SoxB1 in embryos of the ascidian Halocynthia roretzi. Zool. Sci., 20:59-67, Morisawa, S. and G. N. Cherr: Acrosome reaction in spermatozoa from hagfish (Agnatha) Eptatreus burgeri and Eptatretus stouti: acrosomal exocytosis and identification of filamentous actin. Develop. 18

Yamamori: Three GnRH systems in the brain and pituitary of a pleuronectiform fish, barfin flounder, Verasper moseri. Cell and Tissue Res., 309: 323-329, 2002. 1184. Amano, M., Y. Oka, T. Yamanome, A.

21 Growth Differ., 44: , Morokuma, J., M. Ueno, H. Kawanishi, H. Saiga and H. Nishida: HrNodal, the ascidian nodal-related gene, is expressed in the left side of the epidermis, and lies upstream of HrPitx. Dev. Genes Evol., 212: , Murabe, N. and M. Hoshi: Re-examination of sibling cross-sterility in the ascidian, Ciona intestinalis: genetic background of the self-sterility. Zool. Sci., 19: , Nagata, T., T. Suzuki, Y. Ohta, M. F. Flajnik and M. Kasahara: The leukocyte common antigen (CD45) of the Pacific hagfish, Eptatretus stoutii: implications for the primordial function of CD45. Immunogenetics, 54: , Namikawa, H.: Epizoic hidrozoan species on the shells of the nassariid gastropods from Sagami Bay, Japan. Mem. Natn. Sci. Mus., Tokyo, (37): , Nishida, H.: Spatio-temporal pattern of MAP kinase activation in embryos of the ascidian, Halocynthia roretzi. Develop. Growth Differ., 45: 27-37, Nishida, H.: Specification of developmental fates in ascidian embryos: molecular approach to maternal determinants and signaling molecules. Internat. Rev. Cytol., 217: , fig. 6.7, Nishida, H.: Patterning the marginal zone of early ascidian embryos: localized maternal mrna and inductive interactions. BioEssays, 24: , : GnRH,,, , Oka, Y.: Physiology and release activity of GnRH neurons. In Progress in Brain Research, Vol. 141 (ed. by I. S. Parhar), Elsevier Science B. V., , Oka, Y.:., 19: , Oka, Y. and H. Abe: Physiology of GnRH neurons and modulation of their activities by GnRH. In Neuroplasticity, Development, and Steroid Hormone Action, CRC Press LLC, , Satou, Y., K. Yagi, K. S. Imai, L. Yamada, H. Nishida and N. Satoh: Macho-1-related genes in Ciona embryos. Dev. Genes Evol., 212: 87-92, Togo, T., J. M. Alderton and R. A. Steinhardt: Long-term potentiation of exocytosis and cell membrane repair in fibroblasts. Mol. Biol. Cell., 14: , Wirsig-Wiechmann, C. R., and Oka, Y: The terminal nerve ganglion cells project to the olfactory mucosa in the dwarf gourami brain. Neurosci. Res., 44: Yoshida, M., M. Ishikawa, H. Izumi, R. De Santis and M. Morisawa: Store-operated calcium channel regulates chemotactic behavior of ascidian sperm. Proc. Natl. Acad. Sci. USA, 100: , Yoshida, M., M. Murata, K. Inaba, and M. Morisawa: A chemoattractant for ascidian spermatozoa is a sulfated steroid. Proc. Natl. Acad. Sci. USA, 99: , [] contribution number 19

22 2002 CNIDARIA TENTACULATA MOLLUSCA SIPUNCULA ANNELIDA ARTHROPODA ECHINODERMATA CHORDATA CHLOROPHYTA Cavernularia obesa 3 Bugula neritina 2 Aplysia kurodai 60 Mytilus edulis 60 Sipunculus nudus 5 Chloeia flava 5 Cypridina hilgendorfii 50 Asterina pectinifera 40 Asterina batheri 60 Asterias amurensis 30 Diadema setosum 12 Pseudocentrotus depressus 219 Anthocidaris crassispina 60 Hemicentrotus pulcherrimus 720 Clypeaster japonicus 148 Ciona savigyi 40 Eptatretus burgeri 203 Triakis scyllium 8 Plotosus lineatus 1 Pseudolabrus sieboldi 2 Bracaluteres ulvarum 1 Rudarius ercodes 5 Stephanolepis cirrhifer 22 Lactoria diaphana 1 Ostracion immaculatus 1 Takifugu pardalis 6 Takifugu niphobles 30 Diodon holocanthus 1 Codium fragile 2 20

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平成１４年度本態性多種化学物質過敏状態の調査研究研究報告書

平成１４年度本態性多種化学物質過敏状態の調査研究研究報告書 226 227 µ 228 Ω 229 230 0 ppb 2000ppb DG subthreshold (µa) 91 ± 59 86 ± 68 max stim. (µa) 755 ± 287 n=37 678 ± 300 max PS (mv) 8.7 ± 3.9 7.7 ± 4.3 n=61 CA1 subthreshold (µa) 36 ± 26 32 ± 13 max stim. (µa)