MultiClamp 700B Protocol Protocol MultiClamp 700B Mode Telegraph Mode Telegraph MultiClamp Commnader Mode(voltage clamp/current clamp) Clampex Protocol Protocol 1.Digitizer-Amplifure Connections: Digidata MultiClamp 700B 2.Configure Telegraphs: Digidata MultiClamp 700B 3.Create Signals: Signal 4.Configure Protocols: Protocol 5.Configure Sequencing Keys: Mode Telegraph COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 1
MultiClamp 700B USB MultiClamp 700B MultiClamp Commander MultiClamp Commnader DEMO MultiClamp 700B MultiClamp Commander Select Device MultiClamp Hardware Scan MultiClamp 700B OK Clampex MultiClamp Commnader COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 2
Digidata MultiClamp 700B MultiClamp 700B Channel 1 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 3
MultiClamp 700B 6 (1 Channel ) COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 4
Clampex Digidata Channel Signal Channel Signal Create Signal Channel Signal Channel Voltage Clamp Current Clamp Channel Channel Voltage Clamp Membrane current Current Clamp Membrane potential Voltage Clamp Membrane potential Current Clamp Membrane current Voltage Clamp Command potential Current Clamp Command Current 6 3 Channel 3 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 5
3 Channle 2 3 Telegraphs COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 6
MultClamp 700 Telegraph MultiClamp Commander Clampex Clampex Telegraph Clampex Signal Telegraph gain gain Clampex gain gain MultiClamp 700B Mode Telegraph MultiClamp Commnader Mode(voltage clamp/current clamp) Clampex Protocol COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 7
Configure/Telegraphed Instrument Channel Primary Output 1 Analog In #0 Input Channels Analog IN #0 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 8
Telegraphed instruments Axon MultiClamp 700B Multiclamp clampex Protocol Start new trial Configure Sequencing Keys COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 9
Amplifier Configuration Serial number Multiclamp Scan Amplifuer output Primary output 1 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 10
Primary output 1 COMMAND telegraph Output Channels Command COMMNAD 1 Digidata Analog OUT Analog OUT #1 Analog OUT #1 Axoclamp900A Channel 1 SCALED OUTPUT telegraph CURRENT OUTPUT telegraph Axoclamp900A Channel 2 SCALED OUTPUT telegraph COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 11
Secondary Output 1 Analog In #1 Input Channels Analog IN #1 Telegraphed Instrument Axon MultiClamp 700B Amplifuer output n Secondary output 1 Serial number COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 12
COMMNAD 1 Digidata Analog OUT Analog OUT #0 Primary Output, Secondary Output, Command Telegraph OK Telegraphed Instrument Lab Bench Signal COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 13
Signal MultiClamp 700B Signal 6 Primary Output 1 Analog IN #0:voltage clamp, current clamp Secondary Output 1 Analog IN #1:voltage clamp, current clamp Command 1 Analog OUT #0:voltage clamp, current clamp Signal Channel Signal scale factor offset Digidata Analog channel Analog channel Signal Channel Digidata BNC Analog IN,Analog OUT,Digital IN,Digital OUT Signal Channel COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 14
Configure/Lab Bench Lab Bench Input Signals Digidata Channels Channle Primary Output 1 Analog IN #0 Analog IN #0 Voltage Clamp Current Clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 15
Signal Signals Add Add Signals Voltage Clamp membrane current Signal Im_prime Im_prime1 OK Signals list Signal Scaling Telegraph COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 16
Telegraph Lab Bench Lab Bench MultiClamp Commnader COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 17
MultiClamp Commander Lab Bench Filter, gain, capacitance compensation Telegraph MultiClamp Commnader Gain MultiClamp Commnader Primary Output 10 0.5V/nA = 5V/nA Lab Bench Scale factor Scale factor gain=1 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 18
Primary Output Voltage Clamp Signal MultiClamp Telegraph Signal Software RC Filter Hardware Signal Conditioning CyberAmp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 19
Current Clamp membrane potential Signal Voltage Clamp Current Clamp Channle Digidata Channels Analog IN #0 Add Vm_prime Vm_prime1 Signal Telegraph MultiClamp Commander IC MultiClamp Commander Lab Bench Telegraph COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 20
Secondary Output 1 voltage clamp current clamp Signal Secondary Output 1 Analog IN #1 Digidata Channels Analog IN #1 Add voltage clamp membrane potential Siganl Vm_sec Vm_sec1 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 21
Analog IN #1 current clamp membrane current Signal Im_sec Im_sec1 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 22
Im_sec MultiClamp Commander IC Secondary Output Menmrane Current Signal MultiClamp Option Gain F10 500M MultiClamp Commander COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 23
Signal Lab Bench output Signals Analog OUT #0 Analog OUT #0 voltage clamp current clamp Channle Add Signal Signal V_clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 24
Telegraph Analog OUT #0 Telegraph Analog IN #0 Analog IN #1 Voltage clamp Command 20mV/V,100mV/V Option 20mV/V Lab Bench COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 25
holding level holding level Protocol current clamp Signal Add Signal I_clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 26
MultiClamp Commander IC Lab Bemch current current clamp 400 pa/v, 2 na/v 400 pa/v COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 27
MultiClamp Commander Telegraph Lab Bench Digidata Channel Signal Scale factor MultiClamp Commnader Scale factor 6 Signal Signal Protocol COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 28
Clampex Protocol Signal protocol protocol Voltage Clamp Current Clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 29
Acquire/New Protocol Protocol Protocol Protocol (untitled) Protocol Acquire/Edit Protocol Clampex Protocol voltage clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 30
Mode/Rate Acquisition Mode Episodic stimulation command Runs/trial 1 Trial delay 0 Sweep/run Sweep ( ) Sweep duration sweep Fast rate Start-to-Start Intervals Sweep Minimum Sweep Sweep Sweep duration COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 31
Inputs Digidata Analog IN Signal Digidata Analog IN Lab Bench Signal voltage clamp membrane current Signal Im_prime voltage clamp membrane potential Signal Vm_prime Channel #0 Im_prime COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 32
Channel #1 Vm_prime Inputs Outputs Digidata Analog OUT Signal voltage clamp command Signal V_clamp Channel #0 V_clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 33
Signal holding level Protocol V_clamp mv pa MultiClamp Commander IC Telegraph Signal MultiClamp Commander OK Protocol editor MultiClamp Commander VC Protocol Editor V_clamp mv holding level -50mV Holding level -50 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 34
Trigger Trigger Start trial with Immediate Immediate Record View only Trigger source Internal Timer trial COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 35
Waveform Channel #0 ANALOG OUT #0 Analog Waveform 10 epoch epoch A-J V_clamp Signal Info V_clamp First level,delta level mv Signal COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 36
Sweep epoch A Type Step First level -50 epoch A sweep -50mV Output holding level-50mv epoch A Delta level 0 0 2 sweep first level epoch A 10 sweep -50mV COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 37
epoch A First duration 50 epoch A 50ms First rate=10khz 10kHz 50ms=500 epoch A epoch B epoch B Type Step COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 38
epoch B First level -100mV epoch B sweep Delta level 20mV sweep 20mV 1sweep 100mV 20mV 10sweep 80mV Final level First duration 100ms Delta duration Delta duration epoch Mode/Rate sweep duration voltage clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 39
Protocol Update Preview Waveform Preview Waveform preview Waveform Preview Maximize Signal COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 40
Protocol (untitled) protocol Acquire/Save Protocol As Save Voltage Clamp 1 protocol Acquire/Open Protocol COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 41
current clamp Protocol voltage clamp Protocol Mode/Rate Inputs current clamp membrane potential Signal Vm_prime Channel #0 Vm_prime current clamp membrane pcurrentl Signal Im_sec Channel #1 Im_sec < > MultiClmap Commander IC COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 42
Outputs Digidata Analog OUT Signal current clamp command Signal I_clamp Channel #0 I_clamp holding level 0 current clamp COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 43
OK Protocol editor Acquire/Save Protocol As Protocol Current Clamp 1 Scope Signal Voltage Clamp 1 Current Clamp 1 protocol Mode Telegraph COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 44
Mode Telegraph MultiClamp Commander Clampex Protocol Mode Telegraph Sequencing key Protocol(V-Clamp 1, I-Clamp 1) COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 45
Configure/Sequencing Keys Sequencing Keys Sequencing Keys Sequencing Keys Startup COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 46
Add Add a key definiton for V-Clamp IN 0 IN0 Analog In #0 Digidata Analog IN #0 MultiClamp Primary Output Telegraph V-Clamp MultiClamp Commander OK COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 47
Sequencing Keys Propaties operation Protocol Action View Run Protocol file Protocol Browse COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 48
Open file Voltage Clamp 1 Open Protocol file Protocol Repetition count 1 Sequencing key Properties Sequencing Sequencing key Sequencing key Sequencing key OK Properties COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 49
current clamp Sequencing key Add I-CLAMP IN 0 MultiClamp Commander IC Sequencing key COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 50
Browse Protocol Current Clamp 1 Properties Sequencing key COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 51
Option Sequencing key Protocol 2 COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 52
Sequencing key Sequencing key MultiClamp 700B PATCH 1U MultiClamp Commander VC,IC Clampex Protocol V-Clamp 1, I-Clamp 1 Scope MultiClamp Online Help COPYLIGHT 2007 INTERMEDICAL CO.,LTD. 53